##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549563_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	504859
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33366147775914	33.0	31.0	34.0	31.0	34.0
2	32.540416235028	34.0	31.0	34.0	31.0	34.0
3	32.62453675184557	34.0	31.0	34.0	31.0	34.0
4	36.09344193131152	37.0	35.0	37.0	35.0	37.0
5	36.139928177966524	37.0	35.0	37.0	35.0	37.0
6	36.12754650308304	37.0	36.0	37.0	35.0	37.0
7	36.11574122675836	37.0	37.0	37.0	35.0	37.0
8	36.033343963364025	37.0	37.0	37.0	35.0	37.0
9	37.88164418184087	39.0	38.0	39.0	35.0	39.0
10	37.39430811375057	39.0	37.0	39.0	34.0	39.0
11	37.344088151345225	39.0	37.0	39.0	34.0	39.0
12	37.317629278669884	39.0	37.0	39.0	34.0	39.0
13	37.32626535329666	39.0	37.0	39.0	34.0	39.0
14	38.6745071396172	40.0	38.0	41.0	35.0	41.0
15	38.72206101109418	40.0	38.0	41.0	35.0	41.0
16	38.78381092542671	40.0	38.0	41.0	35.0	41.0
17	38.74164865833827	40.0	38.0	41.0	35.0	41.0
18	38.38192247736497	39.0	38.0	41.0	35.0	41.0
19	38.02550415066385	39.0	37.0	41.0	35.0	41.0
20	37.52726404798172	39.0	35.0	41.0	34.0	41.0
21	37.45057927064784	39.0	35.0	41.0	34.0	41.0
22	37.41157035924882	39.0	35.0	41.0	34.0	41.0
23	37.267029012060796	39.0	35.0	41.0	33.0	41.0
24	37.16840345522215	39.0	35.0	41.0	33.0	41.0
25	37.08256364648347	38.0	35.0	41.0	33.0	41.0
26	37.02954092132655	38.0	35.0	41.0	33.0	41.0
27	36.94019914471169	38.0	35.0	41.0	33.0	41.0
28	36.828801705030514	38.0	35.0	41.0	33.0	41.0
29	36.58071857686998	38.0	35.0	40.0	33.0	41.0
30	36.26744893128576	38.0	35.0	40.0	31.0	41.0
31	35.88657625198323	38.0	35.0	40.0	30.0	41.0
32	35.37437779657291	38.0	35.0	41.0	25.0	41.0
33	34.66535805046558	38.0	35.0	41.0	20.0	41.0
34	34.13325106613926	38.0	34.0	41.0	16.0	41.0
35	33.76712111698514	38.0	34.0	41.0	15.0	41.0
36	33.537603568521114	38.0	33.0	41.0	10.0	41.0
37	33.4055647220313	38.0	33.0	41.0	10.0	41.0
38	33.266830936954676	38.0	33.0	41.0	10.0	41.0
39	33.198568313132974	38.0	33.0	40.0	10.0	41.0
40	33.0623837546721	38.0	33.0	40.0	10.0	41.0
41	32.92978237488091	38.0	33.0	40.0	10.0	41.0
42	32.735908441762945	37.0	32.0	40.0	10.0	41.0
43	32.6251983227	37.0	32.0	40.0	10.0	41.0
44	32.43111641072062	37.0	31.0	40.0	10.0	41.0
45	32.40265697947348	37.0	31.0	40.0	10.0	41.0
46	32.24711850239374	37.0	31.0	40.0	10.0	41.0
47	32.21144121427963	36.0	31.0	40.0	10.0	41.0
48	32.07242814330338	36.0	31.0	40.0	8.0	41.0
49	32.03551288577603	36.0	31.0	40.0	8.0	41.0
50	31.879716911058335	36.0	30.0	40.0	8.0	41.0
51	31.093097280626868	35.0	28.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	7.0
11	13.0
12	13.0
13	15.0
14	12.0
15	36.0
16	54.0
17	93.0
18	207.0
19	408.0
20	648.0
21	983.0
22	1812.0
23	3124.0
24	5816.0
25	11209.0
26	17146.0
27	19094.0
28	16666.0
29	14036.0
30	12184.0
31	12421.0
32	13343.0
33	16576.0
34	25599.0
35	33203.0
36	41255.0
37	50920.0
38	89270.0
39	118638.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.844001988674066	4.634561332966234	35.37502550216991	37.14641117618979
2	40.527751312742765	4.709631798185236	41.93091536448791	12.83170152458409
3	18.886659443527797	4.667243725475826	60.44974933595321	15.99634749504317
4	15.172553128695338	4.829665312493192	61.42863651039201	18.56914504841946
5	13.781867808635678	5.724964792149887	60.859962880725114	19.63320451848932
6	17.190146159620806	6.496269255376254	63.30916156788331	13.004423017119631
7	66.21056572231059	2.3994818355223932	28.058329157249844	3.3316232849171747
8	67.12864383917093	2.751461299095391	26.860965140762072	3.2589297209715977
9	62.333839745354645	4.834617190146159	28.462996599050427	4.368546465448769
10	29.214097401452683	21.060335658074827	35.38037353003512	14.345193410437371
11	21.726264164846025	20.81947632903444	40.63748492153255	16.816774584586984
12	19.125538021506994	18.47208824642128	45.16350109634571	17.238872635726015
13	18.61272157176558	18.336406798729943	46.87487001321161	16.176001616292865
14	17.478741589235806	19.665688835892794	46.093661794679306	16.761907780192093
15	16.731404213849807	19.742541977066864	46.59182068656793	16.934233122515398
16	17.4638859562769	18.833377239981854	46.20339540346909	17.499341400272154
17	17.72613739677811	18.64441358874458	45.03613880311136	18.593310211365946
18	17.94718921520662	18.504572563824752	45.50022877674756	18.048009444221062
19	18.815154330218935	20.240106643637134	43.92137210587511	17.023366920268828
20	20.334588469255774	20.803828395651063	42.54118476643974	16.32039836865343
21	19.98637243269903	21.444799439051298	42.472650779722656	16.096177348527014
22	19.224377499460246	19.88872140538249	42.58931701722659	18.29758407793067
23	18.06048817590654	21.433509158002533	41.942403720642794	18.563598945448135
24	17.783381102446423	20.600603336773236	43.28040106247487	18.335614498305468
25	18.028003858503066	21.73874289653151	41.682925331627246	18.55032791333818
26	17.261651272929672	22.239674839905796	42.24308173173104	18.255592155433497
27	17.426449761220457	21.378444278501522	42.76263273508049	18.43247322519753
28	16.882733594924524	22.071310999704867	42.81908414032433	18.22687126504628
29	18.03354996147439	21.583055863122176	41.84455461822014	18.53883955718329
30	18.853778975912086	21.404986342721433	41.90397715005576	17.837257531310723
31	18.61589077346348	23.522211152024624	39.44685545865281	18.415042615859083
32	18.370277641876246	23.743857195771493	38.39269974388888	19.493165418463374
33	19.03541384822297	23.915984462988675	36.6064584369101	20.442143251878246
34	18.915182258808898	24.315700027136288	35.77157186461963	20.99754584943519
35	19.13425332617622	26.072031993091137	34.20618430096324	20.5875303797694
36	19.279244303855137	27.26384990660759	33.37783420717468	20.0790715823626
37	19.536741941809495	27.405473607482485	33.232050929071285	19.825733521636735
38	19.37273575394318	27.175112259066392	32.30189023073769	21.150261756252736
39	19.849700609477104	26.641497923182513	32.002004520073925	21.506796947266466
40	21.00685537942277	25.616855399230282	33.17579759893356	20.200491622413384
41	19.405220071346655	26.348940991445136	33.06467746440095	21.181161472807258
42	19.525451660760726	27.69604978815867	32.4393543543841	20.339144196696505
43	19.15148586040855	26.617332760236028	33.91382544433198	20.317355935023443
44	19.583487666853514	26.114221990694432	33.39843401821103	20.903856324241026
45	20.579013150206297	25.305877482623863	32.71982870464823	21.395280662521614
46	20.17692068478526	25.762242527121433	33.22472215014489	20.836114637948416
47	19.292911486177328	26.62961341681539	33.90293131349545	20.17454378351183
48	19.053042532667536	25.959921483027937	34.79149623954411	20.195539744760417
49	19.77344169362139	24.94042891183479	34.39752485347394	20.88860454106988
50	19.116426566625535	24.692042728761894	34.31987941187539	21.87165129273718
51	18.684424760180566	24.03126417474978	33.80428991064832	23.480021154421333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	644.0
1	1449.0
2	2254.0
3	18312.0
4	34370.0
5	22453.5
6	10537.0
7	10134.0
8	9731.0
9	9649.0
10	9567.0
11	9295.5
12	9024.0
13	8650.5
14	8277.0
15	7777.0
16	7277.0
17	7021.5
18	6766.0
19	6398.5
20	6031.0
21	5789.0
22	5547.0
23	5408.5
24	5270.0
25	5497.5
26	5872.0
27	6019.0
28	6429.5
29	6840.0
30	7281.0
31	7722.0
32	8425.0
33	9128.0
34	10220.5
35	11313.0
36	11975.0
37	12637.0
38	13858.0
39	15079.0
40	16431.0
41	17783.0
42	19483.5
43	21184.0
44	22733.0
45	24282.0
46	27343.0
47	30404.0
48	32445.5
49	34487.0
50	34411.5
51	34336.0
52	32055.5
53	29775.0
54	26892.5
55	24010.0
56	22336.5
57	20663.0
58	19587.5
59	18512.0
60	17180.0
61	15848.0
62	14257.5
63	12667.0
64	11348.5
65	10030.0
66	8380.5
67	6731.0
68	5711.0
69	4691.0
70	4078.5
71	3466.0
72	2947.0
73	2428.0
74	2017.0
75	1337.0
76	1068.0
77	874.0
78	680.0
79	458.0
80	236.0
81	184.0
82	132.0
83	90.0
84	48.0
85	32.0
86	16.0
87	14.5
88	13.0
89	7.0
90	1.0
91	2.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	504859.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.45594003841966
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.20120416040612	31.81027138734724
2	8.929923507075129	7.761164409422619
3	4.6309789152265814	6.037306261778162
4	2.9199591525633894	5.075582793937174
5	2.123124562494865	4.613118684093641
6	1.668765690055304	4.3510669059129174
7	1.2541097597181252	3.8148962963935413
8	1.0029893192327406	3.486867497260266
9	0.7873105207543467	3.079198690336606
>10	3.4044996383854254	22.32126514866289
>50	0.056014538326775734	1.6209140768957322
>100	0.020201964641134548	1.82243096720923
>500	4.591355600257852E-4	0.14644890846634276
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	4.591355600257852E-4	4.059467972283665
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20346	4.030036109091846	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC	734	0.14538712789115377	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.942253183562143E-4	0.0	0.0	0.07091088799050824	0.0
2	5.942253183562143E-4	0.0	0.0	0.2265979213998364	0.0
3	5.942253183562143E-4	0.0	0.0	0.3591101673932722	0.0
4	5.942253183562143E-4	0.0	0.0	0.5569871984058915	0.0
5	5.942253183562143E-4	0.0	0.0	1.0632671696453861	0.0
6	5.942253183562143E-4	0.0	0.0	1.8048603669539416	0.0
7	5.942253183562143E-4	0.0	0.0	2.3984914599917997	0.0
8	5.942253183562143E-4	0.0	0.0	3.1933668608462957	0.0
9	5.942253183562143E-4	0.0	0.0	3.6804335467922726	0.0
10	5.942253183562143E-4	0.0	0.0	4.434109325574071	0.0
11	5.942253183562143E-4	0.0	0.0	5.828359997543869	0.0
12	5.942253183562143E-4	0.0	0.0	6.992447396203692	0.0
13	5.942253183562143E-4	0.0	0.0	7.397114838004274	0.0
14	5.942253183562143E-4	0.0	0.0	7.57993816095187	0.0
15	5.942253183562143E-4	0.0	0.0	7.790491998756089	0.0
16	5.942253183562143E-4	0.0	0.0	8.22803990817238	0.0
17	5.942253183562143E-4	0.0	0.0	8.752146638962563	0.0
18	5.942253183562143E-4	0.0	0.0	9.288732101438223	0.0
19	5.942253183562143E-4	0.0	0.0	9.60802917250163	0.0
20	5.942253183562143E-4	0.0	0.0	9.926930093352798	0.0
21	5.942253183562143E-4	0.0	0.0	10.27831533160744	0.0
22	7.923004244749524E-4	0.0	0.0	10.625540992633587	0.0
23	7.923004244749524E-4	0.0	0.0	10.903440366518176	0.0
24	7.923004244749524E-4	0.0	0.0	11.087254064996365	0.0
25	7.923004244749524E-4	0.0	0.0	11.221152836732632	0.0
26	7.923004244749524E-4	0.0	0.0	11.354259308044425	0.0
27	7.923004244749524E-4	0.0	0.0	11.489742680629641	0.0
28	7.923004244749524E-4	0.0	0.0	11.625424128320978	0.0
29	7.923004244749524E-4	0.0	0.0	11.755559473040988	0.0
30	7.923004244749524E-4	0.0	0.0	11.895994723279173	0.0
31	7.923004244749524E-4	0.0	0.0	12.028705044378727	0.0
32	7.923004244749524E-4	0.0	0.0	12.186174753743124	0.0
33	9.903755305936904E-4	0.0	0.0	12.327204229299666	0.0
34	9.903755305936904E-4	0.0	0.0	12.46447027783995	0.0
35	9.903755305936904E-4	0.0	0.0	12.596784448727268	0.0
36	9.903755305936904E-4	0.0	0.0	12.722165990900429	0.0
37	9.903755305936904E-4	0.0	0.0	12.859035889228478	0.0
38	9.903755305936904E-4	0.0	0.0	13.001055740315612	0.0
39	9.903755305936904E-4	0.0	0.0	13.169419580516541	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAACGTC	25	3.8876864E-5	45.000004	32
CTATCGG	20	7.029524E-4	45.0	2
AACCCGA	35	1.2101191E-7	45.0	24
CACGCGA	20	7.029524E-4	45.0	21
GGATACG	20	7.029524E-4	45.0	9
GACCCGA	35	1.2101191E-7	45.0	40
CGTTTTT	10380	0.0	43.93786	1
GCTTGCG	105	0.0	40.714287	1
CTACGAA	85	0.0	39.705883	11
TACGAAT	85	0.0	39.705883	12
AGCTACG	85	0.0	39.705883	9
CGAATAT	80	0.0	39.375	14
CGATTCG	75	0.0	39.0	10
GTTTTTT	12265	0.0	38.89115	2
CTCGTAA	35	6.241331E-6	38.571426	33
CGCATGG	35	6.241331E-6	38.571426	2
GCTCGTA	35	6.241331E-6	38.571426	32
TGGGCGA	840	0.0	38.30357	6
GCGATTC	195	0.0	38.076923	9
TACACGC	150	0.0	37.5	35
>>END_MODULE