##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549561_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	100797
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.921594888736767	33.0	31.0	34.0	30.0	34.0
2	32.11044971576535	33.0	31.0	34.0	30.0	34.0
3	32.26658531503914	34.0	31.0	34.0	30.0	34.0
4	35.78719604750141	37.0	35.0	37.0	35.0	37.0
5	35.86492653551197	37.0	35.0	37.0	35.0	37.0
6	35.80879391251724	37.0	35.0	37.0	35.0	37.0
7	35.787275414942904	37.0	35.0	37.0	35.0	37.0
8	35.647856583033224	37.0	35.0	37.0	33.0	37.0
9	37.49298094189311	39.0	37.0	39.0	35.0	39.0
10	37.01166701389922	39.0	37.0	39.0	33.0	39.0
11	36.9498298560473	39.0	37.0	39.0	33.0	39.0
12	37.0259233905771	39.0	37.0	39.0	33.0	39.0
13	37.03957459051361	39.0	37.0	39.0	33.0	39.0
14	38.22070101292697	40.0	38.0	41.0	33.0	41.0
15	38.18784289214957	40.0	37.0	41.0	33.0	41.0
16	38.34005972399972	40.0	38.0	41.0	34.0	41.0
17	38.33024792404536	40.0	38.0	41.0	34.0	41.0
18	38.17480678988462	39.0	38.0	41.0	34.0	41.0
19	37.9763385815054	39.0	37.0	41.0	34.0	41.0
20	37.57449130430469	39.0	35.0	41.0	34.0	41.0
21	37.478278123356844	39.0	35.0	41.0	33.0	41.0
22	37.46771233270832	39.0	35.0	41.0	33.0	41.0
23	37.31822375665942	39.0	35.0	40.0	33.0	41.0
24	37.24237824538429	39.0	35.0	41.0	33.0	41.0
25	37.135004017976726	39.0	35.0	40.0	33.0	41.0
26	37.11242398087245	39.0	35.0	40.0	33.0	41.0
27	37.03889004633074	39.0	35.0	40.0	33.0	41.0
28	37.00946456739784	39.0	35.0	40.0	33.0	41.0
29	36.852624582080814	39.0	35.0	40.0	32.0	41.0
30	36.661309364366005	39.0	35.0	40.0	31.0	41.0
31	36.436143932855146	39.0	35.0	40.0	31.0	41.0
32	36.21935176642162	39.0	35.0	40.0	30.0	41.0
33	35.840947647251404	39.0	35.0	40.0	28.0	41.0
34	35.5693721043285	39.0	35.0	41.0	25.0	41.0
35	35.373036895939364	39.0	35.0	41.0	24.0	41.0
36	35.24142583608639	39.0	35.0	41.0	23.0	41.0
37	35.13374405984305	39.0	35.0	41.0	23.0	41.0
38	35.003591376727485	38.0	35.0	40.0	22.0	41.0
39	34.938004107265094	38.0	35.0	40.0	22.0	41.0
40	34.78387253588897	38.0	35.0	40.0	21.0	41.0
41	34.637399922616744	38.0	34.0	40.0	20.0	41.0
42	34.42683810034029	38.0	34.0	40.0	18.0	41.0
43	34.29294522654444	38.0	34.0	40.0	18.0	41.0
44	34.22734803615187	38.0	34.0	40.0	18.0	41.0
45	34.20671250136413	38.0	34.0	40.0	18.0	41.0
46	33.971874162921516	38.0	33.0	40.0	18.0	41.0
47	33.89825094000813	37.0	33.0	40.0	18.0	41.0
48	33.77880294056371	37.0	33.0	40.0	18.0	41.0
49	33.827752810103476	37.0	33.0	40.0	18.0	41.0
50	33.66925602944532	37.0	33.0	40.0	18.0	41.0
51	32.77521156383622	36.0	31.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	3.0
10	4.0
11	2.0
12	6.0
13	6.0
14	3.0
15	9.0
16	6.0
17	18.0
18	46.0
19	79.0
20	121.0
21	229.0
22	338.0
23	482.0
24	768.0
25	1339.0
26	1985.0
27	2292.0
28	2364.0
29	2182.0
30	2231.0
31	2633.0
32	2881.0
33	3613.0
34	5502.0
35	7316.0
36	9561.0
37	12274.0
38	20732.0
39	21761.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.391628719108706	5.929739972419814	40.31369981249442	28.364931495977064
2	32.29957240790897	6.310703691578122	43.36438584481681	18.025338055696103
3	19.505540839509113	6.055735785787276	54.901435558597974	19.53728781610564
4	16.191950157246744	5.641040903995158	55.179221603817574	22.987787334940524
5	13.932954353800211	7.158943222516544	55.5869718344792	23.321130589204046
6	18.1642310783059	8.750260424417393	59.18529321309166	13.900215284185045
7	68.75700665694416	3.5457404486244632	22.93024593985932	4.767006954572061
8	68.30659642648095	4.281873468456403	21.431193388692122	5.980336716370527
9	62.772701568499066	6.146016250483646	24.07512128337153	7.006160897645763
10	30.421540323620743	23.689197099120012	29.647707769080426	16.241554808178815
11	19.5531613044039	19.95495897695368	38.20649424090002	22.28538547774239
12	17.733662708215522	16.740577596555454	43.620345843626296	21.905413851602727
13	20.585930136809626	17.597745964661645	45.672986299195415	16.143337599333314
14	18.44796968163735	19.564074327608957	43.43879282121492	18.549163169538776
15	14.797067373036896	19.56010595553439	45.301943510223516	20.340883161205195
16	14.755399466253957	18.757502703453476	42.66396817365597	23.823129656636606
17	15.079813883349702	19.637489210988424	41.342500272825575	23.940196632836294
18	16.15623480857565	18.562060378781116	42.30483050090776	22.976874311735468
19	16.574898062442333	21.384565016815976	42.93977003283828	19.100766887903408
20	17.631477127295454	23.009613381350636	41.12423980872447	18.23466968262944
21	16.665178527138703	25.053324999751975	40.19564074327609	18.085855729833227
22	14.665119001557587	22.13756361796482	39.940672837485245	23.25664454299235
23	14.705794815321886	24.413425002728257	37.245156105836486	23.635624076113377
24	15.729634810559839	22.94314314910166	37.68961377818784	23.637608262150657
25	15.517326904570572	24.747760350010417	35.58240820659345	24.15250453882556
26	14.730597140787921	25.136660813317857	37.36321517505481	22.76952687083941
27	15.983610623332043	23.93722035378037	39.05076539976388	21.028403623123705
28	14.114507376211593	22.505630127880792	39.25116818952945	24.128694306378165
29	16.05008085558102	21.090905483298116	37.4515114537139	25.407502207406967
30	19.271406887109734	22.182207803803685	36.233221226822224	22.313164082264354
31	18.431104100320447	26.763693363889796	31.393791481889345	23.411411053900412
32	16.699901782791155	27.669474289909424	30.394753812117425	25.235870115182003
33	18.300147821859778	24.11083663204262	30.179469627072237	27.40954591902537
34	18.16224689226862	23.44315803049694	29.841165907715506	28.553429169518935
35	17.861642707620266	23.779477563816386	29.42448683988611	28.93439288867724
36	17.834856196116945	24.580096629860016	30.678492415448872	26.906554758574163
37	16.826889689177257	27.70122126650595	31.53268450449914	23.939204539817656
38	18.111650148317906	28.309374286933142	27.623838011051916	25.955137553697032
39	18.44697758861871	28.37485242616348	26.964096153655365	26.214073831562445
40	19.767453396430447	26.10395150649325	28.657598936476287	25.470996160600016
41	20.265484091788448	27.9323789398494	28.064327311328714	23.73780965703344
42	21.639532922606826	27.706181731599155	29.23896544540016	21.41531990039386
43	18.44697758861871	26.791471968411756	32.091232873994265	22.670317568975268
44	18.637459448197863	24.113812911098545	30.867982182009385	26.380745458694204
45	21.077016181037134	22.48380408147068	28.91355893528577	27.52562080220641
46	22.860799428554422	23.83900314493487	30.704286833933548	22.59591059257716
47	18.361657589015547	27.36291754714922	33.27083147315892	21.00459339067631
48	18.04319573003165	27.295455221881603	32.645812871414826	22.015536176671922
49	20.90736827484945	23.113783148307984	34.21331984086828	21.765528735974286
50	20.79526176374297	20.97185432106114	31.809478456700102	26.42340545849579
51	19.072988283381452	20.782364554500628	30.114983580860542	30.029663581257378
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	315.0
1	510.0
2	705.0
3	2837.0
4	4969.0
5	3204.5
6	1440.0
7	1353.5
8	1267.0
9	1228.5
10	1190.0
11	1154.5
12	1119.0
13	1099.0
14	1079.0
15	976.0
16	873.0
17	832.0
18	791.0
19	765.0
20	739.0
21	708.0
22	677.0
23	673.0
24	669.0
25	685.0
26	740.5
27	780.0
28	837.0
29	894.0
30	974.5
31	1055.0
32	1176.5
33	1298.0
34	1475.0
35	1652.0
36	1770.5
37	1889.0
38	2128.0
39	2367.0
40	2690.5
41	3014.0
42	3536.0
43	4058.0
44	4839.0
45	5620.0
46	7060.5
47	8501.0
48	10256.0
49	12011.0
50	11974.0
51	11937.0
52	10223.5
53	8510.0
54	6792.5
55	5075.0
56	4168.5
57	3262.0
58	3029.5
59	2797.0
60	2593.5
61	2390.0
62	2266.5
63	2143.0
64	1891.5
65	1640.0
66	1416.5
67	1193.0
68	999.5
69	806.0
70	672.0
71	538.0
72	469.0
73	400.0
74	302.0
75	150.5
76	97.0
77	85.0
78	73.0
79	55.0
80	37.0
81	24.5
82	12.0
83	7.5
84	3.0
85	3.0
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	100797.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.382838774963545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.04125970448284	49.46476581644295
2	12.49060856498873	15.833804577517189
3	4.5282369146005506	8.610375308788951
4	2.0207237665915354	5.1231683482643335
5	1.051840721262209	3.3334325426351974
6	0.5807037315301777	2.2083990594958185
7	0.3271349862258953	1.4514320862724088
8	0.20191585274229903	1.0238399952379535
9	0.14087152516904583	0.8035953450995565
>10	0.5760080140245429	6.23232834310545
>50	0.02191334835962935	0.9653065071381093
>100	0.01721763085399449	1.9524390606863298
>500	0.0	0.0
>1k	0.0015652391685449536	2.9971130093157536
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3021	2.9971130093157536	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	257	0.254967905790847	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	253	0.25099953371628125	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	251	0.24901534767899838	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	237	0.2351260454180184	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	167	0.16567953411311845	Illumina Single End Adapter 2 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	158	0.15675069694534557	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	150	0.14881395279621418	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	144	0.1428613946843656	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	134	0.13294046449795133	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.11508279016240563	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	101	0.10020139488278422	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.27183348710775124	0.0
2	0.0	0.0	0.0	0.9702669722313164	0.0
3	0.0	0.0	0.0	1.3710725517624534	0.0
4	0.0	0.0	0.0	2.122086966874014	0.0
5	0.0	0.0	0.0	3.7114199827375813	0.0
6	0.0	0.0	0.0	5.2322985803148905	0.0
7	0.0	0.0	0.0	6.56666369038761	0.0
8	0.0	0.0	0.0	8.652043215571892	0.0
9	0.0	0.0	0.0	9.611397164598152	0.0
10	0.0	0.0	0.0	11.817812038056688	0.0
11	0.0	0.0	0.0	16.47469666755955	0.0
12	0.0	0.0	0.0	21.22781431987063	0.0
13	0.0	0.0	0.0	22.633610127285536	0.0
14	0.0	0.0	0.0	23.13759338075538	0.0
15	0.0	0.0	0.0	23.813208726450192	0.0
16	0.0	0.0	0.0	25.479924997767792	0.0
17	0.0	0.0	0.0	27.560344057858863	0.0
18	0.0	0.0	0.0	29.499885909302858	0.0
19	0.0	0.0	0.0	30.761828229014753	0.0
20	0.0	0.0	0.0	31.79558915443912	0.0
21	0.0	0.0	0.0	33.03173705566634	0.0
22	0.0	0.0	0.0	34.2401063523716	0.0
23	0.0	0.0	0.0	35.021875651061045	0.0
24	0.0	0.0	0.0	35.53974820679187	0.0
25	0.0	0.0	0.0	35.84035239144022	0.0
26	0.0	0.0	0.0	36.0863914600633	0.0
27	0.0	0.0	0.0	36.28977052888479	0.0
28	0.0	0.0	0.0	36.51398355109775	0.0
29	0.0	0.0	0.0	36.72232308501245	0.0
30	0.0	0.0	0.0	36.97332261872873	0.0
31	0.0	0.0	0.0	37.16380447830789	0.0
32	0.0	0.0	0.0	37.34734168675655	0.0
33	0.0	0.0	0.0	37.523934244074724	0.0
34	0.0	0.0	0.0	37.678700754982785	0.0
35	0.0	0.0	0.0	37.82553052174172	0.0
36	0.0	0.0	0.0	38.02494121848864	0.0
37	0.0	0.0	0.0	38.20550214788138	0.0
38	0.0	0.0	0.0	38.366221216901295	0.0
39	0.0	0.0	0.0	38.52495609988392	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTGCG	35	1.1972406E-7	45.000004	1
CGGGATA	35	1.1972406E-7	45.000004	6
TGGGACC	90	0.0	45.000004	6
TGATCAG	20	7.002651E-4	45.0	14
GGGTGTA	20	7.002651E-4	45.0	7
TCGTTTG	20	7.002651E-4	45.0	1
GGAGATA	20	7.002651E-4	45.0	8
TTTACGG	20	7.002651E-4	45.0	2
GTCCTAG	20	7.002651E-4	45.0	1
ATGTGCT	20	7.002651E-4	45.0	11
ATAGCAT	20	7.002651E-4	45.0	10
TTCGTTG	20	7.002651E-4	45.0	1
TACGGGA	25	3.8654343E-5	45.0	4
GGATAGC	40	6.708433E-9	45.0	8
GGATAAT	20	7.002651E-4	45.0	8
ACGGGAT	20	7.002651E-4	45.0	5
GCACCAC	25	3.8654343E-5	45.0	29
GTTCGGG	25	3.8654343E-5	45.0	3
GTGCTAG	20	7.002651E-4	45.0	1
AATTGAT	20	7.002651E-4	45.0	11
>>END_MODULE