FastQCFastQC Report
Sat 18 Jun 2016
SRR3549558_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549558_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169466
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56403.3281012120425335No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC7320.4319450509246692No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT5670.33458038780640365Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC4780.2820624786092785Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC3820.2254139473404695RNA PCR Primer, Index 40 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC3700.2183328809318683RNA PCR Primer, Index 40 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC3550.20948154792111692No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3410.2012203037777489No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.1315898174265044No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2120.12509883988528672No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1820.10739617386378389No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTGTG351.2037526E-745.01
TTTACGG253.8766942E-545.02
TTTCGCG207.016254E-445.01
CGTCTGG207.016254E-445.02
AGGGTCT207.016254E-445.06
TACGGGG351.2037526E-745.04
GGCCACG207.016254E-445.019
GCCGATT253.8766942E-545.09
CTACGGG207.016254E-445.03
CGGGATC351.2037526E-745.06
TTACGGG351.2037526E-745.03
TGCTAGG253.8766942E-545.02
GTCTTCG207.016254E-445.01
GGTCTAT207.016254E-445.08
TGGTGAC207.016254E-445.014
CCACGTG253.8766942E-545.021
GCTTTAG351.2037526E-745.01
CCTACCA302.154522E-644.9999967
ATATACT302.154522E-644.99999617
CTACCAC302.154522E-644.9999968
Produced by FastQC (version 0.11.5)