##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549558_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	169466
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05873744585934	33.0	31.0	34.0	30.0	34.0
2	32.22336633897065	34.0	31.0	34.0	30.0	34.0
3	32.35477912973694	34.0	31.0	34.0	30.0	34.0
4	35.915322247530476	37.0	35.0	37.0	35.0	37.0
5	35.91948237404553	37.0	35.0	37.0	35.0	37.0
6	35.87391571170618	37.0	35.0	37.0	35.0	37.0
7	35.90992293439392	37.0	35.0	37.0	35.0	37.0
8	35.702813543719685	37.0	35.0	37.0	35.0	37.0
9	37.54363117085433	39.0	37.0	39.0	35.0	39.0
10	37.05489596733268	39.0	37.0	39.0	33.0	39.0
11	37.055303128651175	39.0	37.0	39.0	33.0	39.0
12	37.08992364250056	39.0	37.0	39.0	33.0	39.0
13	37.06730553621375	39.0	37.0	39.0	33.0	39.0
14	38.13129477299281	40.0	37.0	41.0	33.0	41.0
15	38.16995739558377	40.0	37.0	41.0	33.0	41.0
16	38.25262884590419	40.0	38.0	41.0	33.0	41.0
17	38.20372818146413	40.0	37.0	41.0	33.0	41.0
18	38.01972076994795	39.0	37.0	41.0	33.0	41.0
19	37.76792985023545	39.0	37.0	41.0	34.0	41.0
20	37.428192085728114	39.0	35.0	40.0	33.0	41.0
21	37.27301641627229	39.0	35.0	40.0	33.0	41.0
22	37.30114005169178	39.0	35.0	40.0	33.0	41.0
23	37.28910223879716	39.0	35.0	40.0	33.0	41.0
24	37.21439108729775	39.0	35.0	40.0	33.0	41.0
25	37.10206767139131	39.0	35.0	40.0	33.0	41.0
26	37.01373136794401	39.0	35.0	40.0	33.0	41.0
27	36.88061912123966	38.0	35.0	40.0	32.0	41.0
28	36.754505328502475	38.0	35.0	40.0	32.0	41.0
29	36.702536201952014	38.0	35.0	40.0	32.0	41.0
30	36.53014764023462	38.0	35.0	40.0	31.0	41.0
31	36.21279784735581	38.0	35.0	40.0	30.0	41.0
32	35.86839838079615	38.0	35.0	40.0	30.0	41.0
33	35.49936270402323	38.0	35.0	40.0	27.0	41.0
34	35.183865790188	38.0	35.0	40.0	24.0	41.0
35	34.943693720274275	38.0	35.0	40.0	23.0	41.0
36	34.64476650183518	38.0	34.0	40.0	21.0	41.0
37	34.44244273187542	38.0	34.0	40.0	21.0	41.0
38	34.42534195649865	38.0	34.0	40.0	21.0	41.0
39	34.348524187742676	38.0	34.0	40.0	20.0	41.0
40	34.14439474584872	38.0	33.0	40.0	18.0	41.0
41	33.99901455159147	38.0	33.0	40.0	18.0	41.0
42	33.90682496784016	38.0	33.0	40.0	18.0	41.0
43	33.742868776037675	37.0	33.0	40.0	18.0	41.0
44	33.607089327652744	37.0	33.0	40.0	17.0	41.0
45	33.525261704412685	37.0	33.0	40.0	18.0	41.0
46	33.184137231067	37.0	32.0	40.0	15.0	41.0
47	32.95686450379427	36.0	32.0	40.0	15.0	41.0
48	32.92760199686073	36.0	32.0	40.0	15.0	41.0
49	32.97248415611391	36.0	32.0	40.0	15.0	41.0
50	32.90427578393306	36.0	32.0	40.0	15.0	41.0
51	31.726535116188497	35.0	30.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	1.0
13	7.0
14	5.0
15	5.0
16	16.0
17	42.0
18	60.0
19	100.0
20	209.0
21	300.0
22	548.0
23	817.0
24	1407.0
25	2408.0
26	3570.0
27	4200.0
28	4134.0
29	4076.0
30	4295.0
31	4694.0
32	5546.0
33	6791.0
34	10335.0
35	13753.0
36	17227.0
37	22608.0
38	33732.0
39	28561.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.777442082777668	5.694357570250079	39.76254823976491	28.765652107207345
2	33.752493125464696	5.340304249820023	42.35185818984339	18.55534443487189
3	20.63658787013324	5.642429749920338	54.51595010208538	19.205032277861044
4	15.722327782564054	5.886726541017078	55.45655175669456	22.93439391972431
5	14.227042592614447	6.59306291527504	55.85899236425006	23.320902127860453
6	18.156444360520695	7.751407361948709	59.891069595081014	14.201078682449578
7	67.94814299033435	3.0950161094260795	24.03727001286394	4.919570887375638
8	66.37378589215535	3.7299517307306482	23.716851757874736	6.179410619239258
9	61.03643208667226	6.054901868221354	26.136216114146794	6.772449930959603
10	28.228671237888424	24.78491260783874	31.70370457790943	15.2827115763634
11	20.94992505871384	19.6411079508574	38.18878123045331	21.22018575997545
12	19.306527563050995	17.15034284163195	43.20040598114076	20.342723614176293
13	21.148194918154676	18.374777241452563	44.71280374824448	15.764224092148277
14	18.63913705404034	20.255390461803547	43.521414324997345	17.58405815915877
15	14.804739593782823	19.445788535753483	45.186055019886	20.5634168505777
16	15.391287927961953	19.474702890255273	42.82215901714798	22.311850164634794
17	14.746320795911863	19.536662221330534	41.74701710077537	23.969999881982226
18	16.57913681800479	17.78409828520175	43.91500359954209	21.721761297251366
19	16.37614624762489	21.28922615745931	43.59635561115504	18.738271983760754
20	17.746922686556594	23.406465013631053	41.37821155866073	17.46840074115162
21	16.628704282865	24.90529073678496	40.97105024016617	17.49495474018387
22	14.417051207911912	22.164327947788937	40.453542303470904	22.965078540828248
23	14.335028855345616	24.12696352070622	38.48323557527763	23.05477204867053
24	15.303954775589204	22.830538279064825	39.12112164091912	22.74438530442685
25	15.442625659424309	25.24400174666305	35.991290288317416	23.322082305595224
26	14.981766253997852	25.09293899661289	37.86659270886196	22.058702040527304
27	15.840345556040738	24.88109709322224	39.11286039677576	20.16569695396127
28	14.049425843532035	24.08565729998938	38.89098698263959	22.973929873839
29	17.323238879775293	22.400363494742308	37.26706241960039	23.009335205882007
30	20.918060259875137	24.94187624656273	35.804232117356875	18.335831376205256
31	20.01109367070681	29.98182526288459	31.011530336468674	18.99555072993993
32	20.387570368097435	28.71136393140807	29.873839000153424	21.02722670034107
33	20.554565517566946	26.95348919547284	28.84885463750841	23.643090649451807
34	21.134622874204855	26.496170323250684	29.73339784971617	22.635808952828295
35	23.964689082175774	26.26308522063423	27.807937875444043	21.964287821745955
36	22.74851592649853	32.70626556359388	26.2247294442543	18.320489065653287
37	20.336232636635078	34.57330673999504	26.922214485501517	18.168246137868362
38	23.211735687394523	31.168493975192664	26.482008190433483	19.137762146979334
39	23.729243624089786	28.412778964512054	26.532755833028453	21.325221578369703
40	23.74045531257007	29.77588424816777	28.090590442920703	18.39306999634145
41	22.758547437244047	29.57171350005311	26.38051290524353	21.28922615745931
42	24.028418679853186	26.414147970684386	27.681068768956607	21.876364580505825
43	21.218415493373303	24.439120531552053	31.311295481099457	23.031168493975194
44	21.115740030448585	25.38916360803937	30.24205445340068	23.25304190811136
45	23.8106758877887	24.284517248297593	28.20329741659094	23.701509447322767
46	24.35650809011837	25.868315768354716	30.26565800809602	19.509518133430895
47	19.553774798484653	28.123635419494175	33.27452114288412	19.048068639137053
48	19.085834326649593	26.190504289946066	33.821533522948556	20.902127860455785
49	22.44521024866345	22.30594927596096	34.24403715199509	21.0048033233805
50	21.082695053875113	20.652520269552593	32.626013477629726	25.63877119894256
51	19.15605490186822	19.957395583774918	30.557751997450815	30.328797516906047
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	508.0
1	951.5
2	1395.0
3	5400.5
4	9406.0
5	5900.0
6	2394.0
7	2275.5
8	2157.0
9	2158.5
10	2160.0
11	2054.5
12	1949.0
13	1848.5
14	1748.0
15	1631.0
16	1514.0
17	1419.5
18	1325.0
19	1277.0
20	1229.0
21	1157.5
22	1086.0
23	1056.0
24	1026.0
25	1082.5
26	1234.5
27	1330.0
28	1511.0
29	1692.0
30	1768.0
31	1844.0
32	2046.5
33	2249.0
34	2564.5
35	2880.0
36	3095.5
37	3311.0
38	3616.0
39	3921.0
40	4677.5
41	5434.0
42	6418.5
43	7403.0
44	8497.0
45	9591.0
46	11800.5
47	14010.0
48	16922.5
49	19835.0
50	19875.0
51	19915.0
52	17312.0
53	14709.0
54	11741.5
55	8774.0
56	7228.5
57	5683.0
58	5032.5
59	4382.0
60	4050.0
61	3718.0
62	3322.0
63	2926.0
64	2559.0
65	2192.0
66	1837.5
67	1483.0
68	1266.5
69	1050.0
70	866.5
71	683.0
72	599.5
73	516.0
74	454.5
75	322.0
76	251.0
77	198.5
78	146.0
79	101.0
80	56.0
81	44.5
82	33.0
83	21.5
84	10.0
85	9.5
86	9.0
87	5.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	169466.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.266354312959535
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.85396766515193	43.43880188356366
2	12.143600523457707	13.908394604227398
3	5.3314373447916985	9.159359399525568
4	2.554432388430348	5.851321208974071
5	1.4085958350077799	4.03325740856573
6	0.7800344163137449	2.68018363565553
7	0.45647984996960234	1.82986557775601
8	0.32252413778890643	1.4775825239281035
9	0.1957814254948633	1.0090519632256618
>10	0.8944119859449545	8.777571902328491
>50	0.0360649994332643	1.4097223041790092
>100	0.01957814254948633	2.3302609372971568
>500	0.002060857110472245	0.7665254387310729
>1k	0.0	0.0
>5k	0.0010304285552361226	3.3281012120425335
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5640	3.3281012120425335	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCC	732	0.4319450509246692	No Hit
CTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGCT	567	0.33458038780640365	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	478	0.2820624786092785	Illumina PCR Primer Index 8 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	382	0.2254139473404695	RNA PCR Primer, Index 40 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGCCGTCTTCTGC	370	0.2183328809318683	RNA PCR Primer, Index 40 (95% over 24bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGAAGAAGCTCGTATGC	355	0.20948154792111692	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	341	0.2012203037777489	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	223	0.1315898174265044	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	212	0.12509883988528672	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	182	0.10739617386378389	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.37942714172754416	0.0
2	0.0	0.0	0.0	1.2503983099854838	0.0
3	0.0	0.0	0.0	1.7354513589746616	0.0
4	0.0	0.0	0.0	2.5108281307164857	0.0
5	0.0	0.0	0.0	4.269883044386485	0.0
6	0.0	0.0	0.0	5.83892934275902	0.0
7	0.0	0.0	0.0	7.1748905385151005	0.0
8	0.0	0.0	0.0	9.263805129052436	0.0
9	0.0	0.0	0.0	10.184343762170583	0.0
10	0.0	0.0	0.0	12.376523904500017	0.0
11	0.0	0.0	0.0	17.31910825770361	0.0
12	0.0	0.0	0.0	22.002053509258495	0.0
13	0.0	0.0	0.0	23.568739452161495	0.0
14	0.0	0.0	0.0	24.09981943280658	0.0
15	0.0	0.0	0.0	24.899979936978507	0.0
16	0.0	0.0	0.0	26.750498625092938	0.0
17	0.0	0.0	0.0	28.96628232211771	0.0
18	0.0	0.0	0.0	30.95783224953678	0.0
19	0.0	0.0	0.0	32.220032336869934	0.0
20	0.0	0.0	0.0	33.2963544309773	0.0
21	0.0	0.0	0.0	34.60930216090543	0.0
22	0.0	0.0	0.0	35.77885829605939	0.0
23	0.0	0.0	0.0	36.624455643019836	0.0
24	0.0	0.0	0.0	37.1207203804893	0.0
25	0.0	0.0	0.0	37.45412059056094	0.0
26	0.0	0.0	0.0	37.707858803535814	0.0
27	0.0	0.0	0.0	37.91970070692646	0.0
28	5.900888673834279E-4	0.0	0.0	38.12269127730636	0.0
29	5.900888673834279E-4	0.0	0.0	38.35164575785113	0.0
30	5.900888673834279E-4	0.0	0.0	38.59712272668264	0.0
31	5.900888673834279E-4	0.0	0.0	38.79185205291917	0.0
32	5.900888673834279E-4	0.0	0.0	39.04913079909834	0.0
33	5.900888673834279E-4	0.0	0.0	39.22910790365029	0.0
34	5.900888673834279E-4	0.0	0.0	39.445670517980005	0.0
35	5.900888673834279E-4	0.0	0.0	39.626237711399334	0.0
36	5.900888673834279E-4	0.0	0.0	39.802674282746985	0.0
37	5.900888673834279E-4	0.0	0.0	39.96317845467527	0.0
38	5.900888673834279E-4	0.0	0.0	40.13902493715553	0.0
39	5.900888673834279E-4	0.0	0.0	40.443510792725384	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTGTG	35	1.2037526E-7	45.0	1
TTTACGG	25	3.8766942E-5	45.0	2
TTTCGCG	20	7.016254E-4	45.0	1
CGTCTGG	20	7.016254E-4	45.0	2
AGGGTCT	20	7.016254E-4	45.0	6
TACGGGG	35	1.2037526E-7	45.0	4
GGCCACG	20	7.016254E-4	45.0	19
GCCGATT	25	3.8766942E-5	45.0	9
CTACGGG	20	7.016254E-4	45.0	3
CGGGATC	35	1.2037526E-7	45.0	6
TTACGGG	35	1.2037526E-7	45.0	3
TGCTAGG	25	3.8766942E-5	45.0	2
GTCTTCG	20	7.016254E-4	45.0	1
GGTCTAT	20	7.016254E-4	45.0	8
TGGTGAC	20	7.016254E-4	45.0	14
CCACGTG	25	3.8766942E-5	45.0	21
GCTTTAG	35	1.2037526E-7	45.0	1
CCTACCA	30	2.154522E-6	44.999996	7
ATATACT	30	2.154522E-6	44.999996	17
CTACCAC	30	2.154522E-6	44.999996	8
>>END_MODULE