SRR3549556_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549556_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132976
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3219	2.420737576705571	No Hit
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	788	0.5925881362050295	TruSeq Adapter, Index 20 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	744	0.5594994585489111	TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	685	0.5151305498736614	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	669	0.5030983034532547	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC	570	0.4286487787269883	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	303	0.2278606665864517	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	269	0.20229214294308745	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	259	0.1947719889303333	TruSeq Adapter, Index 23 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	218	0.16393935747804114	No Hit
GGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	199	0.14965106485380822	No Hit
GCTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	177	0.133106726025749	No Hit
GTTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	175	0.13160269522319817	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	174	0.13085067982192275	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	167	0.1255865720129948	TruSeq Adapter, Index 20 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	158	0.11881843340151607	TruSeq Adapter, Index 20 (95% over 21bp)
GGGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	153	0.11505835639513896	No Hit
GGGACTGTCTAGGGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGT	151	0.11355432559258813	No Hit
TTTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	139	0.1045301407772831	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTGC	20	7.0107734E-4	45.000004	20
ACACGTG	20	7.0107734E-4	45.000004	42
TTGTTAG	20	7.0107734E-4	45.000004	1
ACACGCG	45	3.8016879E-10	45.000004	36
ACACGAC	20	7.0107734E-4	45.000004	26
GTGCATG	20	7.0107734E-4	45.000004	1
TTCGTTG	20	7.0107734E-4	45.000004	1
AAACACG	20	7.0107734E-4	45.000004	40
CACGCGG	45	3.8016879E-10	45.000004	37
CACGTGA	20	7.0107734E-4	45.000004	43
GGGTACG	20	7.0107734E-4	45.000004	7
CACCGAT	20	7.0107734E-4	45.000004	10
GGCGATA	20	7.0107734E-4	45.000004	8
CACGACC	20	7.0107734E-4	45.000004	27
GACACGA	20	7.0107734E-4	45.000004	25
CTGCGGG	45	3.8016879E-10	45.000004	3
CGGGATA	20	7.0107734E-4	45.000004	6
TGCCAGT	20	7.0107734E-4	45.000004	24
TGCCACA	20	7.0107734E-4	45.000004	11
GCTTGTC	20	7.0107734E-4	45.000004	38

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content