FastQCFastQC Report
Sat 18 Jun 2016
SRR3549555_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549555_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132713
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32202.4262883063452714No Hit
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC7710.5809528832895045TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT7370.555333689992691TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC6910.5206724284734728TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC6440.48525766126905423TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC5720.43100525193462585No Hit
TCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG3120.2350937737825232TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC2810.21173509754131098No Hit
GCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG2600.19591147815210264TruSeq Adapter, Index 23 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT1850.13939855176207305No Hit
GGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT1830.13789154039167226No Hit
GCTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC1730.1303564835396683No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG1600.12056090963206316TruSeq Adapter, Index 20 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1550.1167933812060612No Hit
TGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG1540.11603987552086081TruSeq Adapter, Index 20 (95% over 21bp)
GTTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC1450.10925832435405725No Hit
GTGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC1380.10398378455765449No Hit
TGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT1350.10172326750205331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTAGC302.150964E-645.00000421
TGTTCTG302.150964E-645.0000041
GTCAAGC302.150964E-645.00000416
TGGGACC1200.045.0000046
TGGGACA650.045.0000046
TCAAGCG302.150964E-645.00000417
CATGGTC406.7375368E-945.012
ATCCTAG453.8016879E-1045.014
ATGGGAT207.010727E-445.05
GAAGCTA207.010727E-445.07
ACACGAC351.2010969E-745.026
GACACGA351.2010969E-745.025
CCCTACA207.010727E-445.027
GGCACCG406.7375368E-945.08
GTGGGTG207.010727E-445.04
TGGGTAG253.8721184E-545.06
TTGTCGG207.010727E-445.02
TCGGGGC253.8721184E-545.05
CGACCAA351.2010969E-745.029
GTCTAGG453.8016879E-1045.07
Produced by FastQC (version 0.11.5)