##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549555_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132713
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75638407691786	33.0	31.0	34.0	30.0	34.0
2	31.954232064680927	33.0	31.0	34.0	30.0	34.0
3	32.134794632025496	33.0	31.0	34.0	30.0	34.0
4	35.693993806183265	37.0	35.0	37.0	33.0	37.0
5	35.76944986549923	37.0	35.0	37.0	35.0	37.0
6	35.7358736521667	37.0	35.0	37.0	35.0	37.0
7	35.701988501503244	37.0	35.0	37.0	35.0	37.0
8	35.5153300731654	37.0	35.0	37.0	33.0	37.0
9	37.30145501947812	39.0	37.0	39.0	34.0	39.0
10	36.910046491300776	39.0	37.0	39.0	32.0	39.0
11	36.91311325943954	39.0	37.0	39.0	33.0	39.0
12	36.94777452095876	39.0	37.0	39.0	33.0	39.0
13	36.906618040433116	39.0	37.0	39.0	33.0	39.0
14	37.88606240534085	39.0	37.0	41.0	33.0	41.0
15	37.89675465101384	39.0	37.0	41.0	33.0	41.0
16	38.06926977764047	40.0	37.0	41.0	33.0	41.0
17	38.10413448569469	40.0	37.0	41.0	33.0	41.0
18	38.011249839880044	39.0	37.0	41.0	33.0	41.0
19	37.885045172665826	39.0	37.0	41.0	34.0	41.0
20	37.523950178204096	39.0	35.0	40.0	33.0	41.0
21	37.46842434426168	39.0	35.0	40.0	33.0	41.0
22	37.454130341413425	39.0	35.0	40.0	33.0	41.0
23	37.344676105581215	39.0	35.0	40.0	33.0	41.0
24	37.267675359610585	39.0	35.0	40.0	33.0	41.0
25	37.185060996285216	39.0	35.0	40.0	33.0	41.0
26	37.17817395432249	39.0	35.0	40.0	33.0	41.0
27	37.11940051087686	39.0	35.0	40.0	33.0	41.0
28	37.0876327111888	39.0	35.0	40.0	33.0	41.0
29	36.921627873682304	39.0	35.0	40.0	32.0	41.0
30	36.7969452879522	39.0	35.0	40.0	32.0	41.0
31	36.57190328001025	38.0	35.0	40.0	31.0	41.0
32	36.43690520144975	38.0	35.0	40.0	31.0	41.0
33	36.16302849004996	39.0	35.0	40.0	30.0	41.0
34	36.02297438834176	39.0	35.0	40.0	30.0	41.0
35	35.91273650659694	39.0	35.0	40.0	29.0	41.0
36	35.72679390866004	39.0	35.0	40.0	27.0	41.0
37	35.52710736702508	38.0	35.0	40.0	26.0	41.0
38	35.347637382924056	38.0	35.0	40.0	25.0	41.0
39	35.47529631611071	38.0	35.0	40.0	25.0	41.0
40	35.46302170849879	39.0	35.0	40.0	25.0	41.0
41	35.4261903506062	39.0	35.0	40.0	25.0	41.0
42	35.2444221741653	38.0	35.0	40.0	24.0	41.0
43	35.084686503959674	38.0	35.0	40.0	23.0	41.0
44	34.983257103674845	38.0	35.0	40.0	23.0	41.0
45	34.94842253584803	38.0	35.0	40.0	23.0	41.0
46	34.682374748517475	38.0	34.0	40.0	23.0	41.0
47	34.59462901147589	38.0	34.0	40.0	23.0	41.0
48	34.539291553954776	38.0	34.0	40.0	22.0	41.0
49	34.6427930948739	38.0	34.0	40.0	23.0	41.0
50	34.504750853345186	38.0	34.0	40.0	22.0	41.0
51	33.488309359294114	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	1.0
12	2.0
13	5.0
14	6.0
15	9.0
16	10.0
17	20.0
18	55.0
19	80.0
20	131.0
21	172.0
22	288.0
23	461.0
24	763.0
25	1286.0
26	2039.0
27	2450.0
28	2546.0
29	2644.0
30	2884.0
31	3340.0
32	4084.0
33	5342.0
34	8092.0
35	10132.0
36	12852.0
37	17341.0
38	29958.0
39	25712.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.86634316155915	5.5164151213520904	44.06953350463029	24.547708212458463
2	31.023336071070656	5.011566312267826	42.71397677695478	21.251120839706736
3	21.083089071907047	5.493056445110878	51.84721918726877	21.576635295713306
4	17.425572475944332	5.6965029801149845	51.559380015522216	25.318544528418467
5	14.275165206121478	6.911907650343221	53.01666001069978	25.796267132835517
6	18.542267901411318	8.008258422309797	57.558038775402565	15.891434900876328
7	67.08385764770594	3.150407269822851	22.852320420757575	6.913414661713622
8	64.82861513190117	3.8052037102619938	22.283423628431276	9.082757529405558
9	57.760731804721466	6.498233029168205	26.106711475138077	9.634323690972248
10	29.228485528923315	20.846488286754123	31.94713404112634	17.977892143196218
11	20.274577471687024	18.3260117697588	37.0212413252658	24.378169433288374
12	19.050884238921583	14.709937986482108	43.75682864527213	22.48234912932418
13	21.878791075478667	16.379706584886183	45.10409681041043	16.63740552922472
14	17.867126807471763	19.17671968835005	43.38535034246833	19.570803161709854
15	13.208201155877722	18.524183764966505	45.17115881639327	23.0964562627625
16	12.918854972760768	18.498564571669693	42.592662361637515	25.98991809393202
17	12.451681447936524	19.070475386736792	40.081227912864605	28.39661525246208
18	13.975269943411723	17.88219692117577	44.30085974998681	23.841673385425693
19	14.17796297273063	21.125285390278272	43.92787443581262	20.768877201178483
20	14.868174180374192	24.38947201856638	40.80911440476818	19.933239396291246
21	14.06343010858017	25.72995863253788	41.17079713366437	19.035814125217577
22	11.50979934143603	24.054161988652204	38.196710194178415	26.23932847573335
23	12.06362602005832	24.090330261541823	36.645241988350804	27.20080173004905
24	14.462034616051177	22.963839262167234	36.35438879386345	26.219737327918143
25	13.571390896144312	25.092492822858347	33.66814102612404	27.6679752548733
26	12.658895511366634	25.906278962874772	37.2126317693067	24.222193756451894
27	15.748268820688253	23.806258618221275	38.43105046227574	22.014422098814734
28	12.742534642423879	21.67835856321536	39.18530965316133	26.393797141199432
29	15.065592669896693	20.641534740379615	38.01888285247112	26.273989737252567
30	22.29171219096848	21.02657614551702	34.895601787315485	21.786109876199017
31	18.91148568715951	26.744177284817617	31.978781279904755	22.36555574811812
32	16.675834319169937	25.672692200462656	31.083616525886686	26.567856954480725
33	20.258753852297815	20.698801172454846	29.008461868844797	30.03398310640254
34	16.950110388582882	17.625251482522437	35.624241784904264	29.800396343990414
35	17.41879092477753	18.667349845154583	34.99431103207674	28.91954819799115
36	21.987295894147522	18.862507817621484	34.095378749632665	25.05481753859833
37	19.96488663506966	21.14487653809348	36.62188331210959	22.268353514727266
38	16.6050047847611	25.22737034050922	31.114510258979905	27.053114615749774
39	17.745812392154498	20.56769118322998	30.040011151884137	31.646485272731383
40	18.62590703246856	16.05193161182401	36.49981539110713	28.822345964600306
41	16.89133694513725	16.931272746452873	33.15500365450257	33.022386653907304
42	18.79695282300905	16.777557586671993	32.93422648873886	31.491263101580103
43	19.17822669972045	17.159584969068593	35.57375690399584	28.088431427215117
44	18.845930692547075	17.838493591434148	33.2077490524666	30.107826663552178
45	22.020450144296337	15.572701996036562	32.18147430922366	30.22537355044344
46	22.589346936622636	16.364636471182177	35.45545651141938	25.59056008077581
47	16.075290288065222	20.106545703887335	38.290144899143264	25.528019108904175
48	15.673671757853413	18.591999276634542	38.038474000286335	27.695854965225713
49	21.439497261006835	14.19830762623104	37.29401038330834	27.068184729453783
50	20.49686164882114	12.285156691507238	34.737365593423405	32.48061606624822
51	17.833219051637744	12.406471106824501	31.667583431916995	38.09272640962076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	196.0
1	454.0
2	712.0
3	3089.0
4	5466.0
5	3466.0
6	1466.0
7	1361.0
8	1256.0
9	1170.0
10	1084.0
11	1093.5
12	1103.0
13	979.5
14	856.0
15	829.5
16	803.0
17	738.0
18	673.0
19	635.0
20	597.0
21	582.5
22	568.0
23	537.0
24	506.0
25	508.0
26	518.5
27	527.0
28	570.0
29	613.0
30	694.0
31	775.0
32	846.0
33	917.0
34	1130.0
35	1343.0
36	1489.5
37	1636.0
38	1892.0
39	2148.0
40	2778.5
41	3409.0
42	4466.0
43	5523.0
44	6781.5
45	8040.0
46	11022.5
47	14005.0
48	17784.0
49	21563.0
50	21465.5
51	21368.0
52	17311.5
53	13255.0
54	9985.5
55	6716.0
56	5180.5
57	3645.0
58	3125.5
59	2606.0
60	2502.0
61	2398.0
62	2146.0
63	1894.0
64	1696.0
65	1498.0
66	1245.5
67	993.0
68	835.0
69	677.0
70	564.5
71	452.0
72	396.5
73	341.0
74	297.0
75	197.5
76	142.0
77	120.0
78	98.0
79	70.0
80	42.0
81	35.5
82	29.0
83	18.5
84	8.0
85	4.5
86	1.0
87	0.5
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	132713.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.819603203906176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.78455581046853	44.19687596542916
2	12.0943678968796	13.743943698055203
3	4.561910697945814	7.776178671268075
4	2.0090972986592757	4.566244452314392
5	0.9654276128210909	2.742760694129437
6	0.5596297425968412	1.9078763949273998
7	0.3726444494542947	1.4821456827891766
8	0.2705319134828332	1.2297212782470444
9	0.2241171244048961	1.1460821471898006
>10	1.0423435490073865	10.53551649047192
>50	0.07691593618629569	3.0260788317647855
>100	0.03050114710835864	2.647065472108987
>500	0.006630684153991009	2.5732219149593485
>1k	0.0013261368307982016	2.4262883063452714
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3220	2.4262883063452714	No Hit
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	771	0.5809528832895045	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	737	0.555333689992691	TruSeq Adapter, Index 20 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	691	0.5206724284734728	TruSeq Adapter, Index 23 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	644	0.48525766126905423	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCC	572	0.43100525193462585	No Hit
TCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	312	0.2350937737825232	TruSeq Adapter, Index 20 (95% over 21bp)
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGC	281	0.21173509754131098	No Hit
GCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	260	0.19591147815210264	TruSeq Adapter, Index 23 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	185	0.13939855176207305	No Hit
GGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	183	0.13789154039167226	No Hit
GCTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	173	0.1303564835396683	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	160	0.12056090963206316	TruSeq Adapter, Index 20 (95% over 21bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	155	0.1167933812060612	No Hit
TGCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	154	0.11603987552086081	TruSeq Adapter, Index 20 (95% over 21bp)
GTTCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	145	0.10925832435405725	No Hit
GTGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTC	138	0.10398378455765449	No Hit
TGCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCT	135	0.10172326750205331	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5824598946599052	0.0
2	0.0	0.0	0.0	2.3230580274728174	0.0
3	0.0	0.0	0.0	3.4322183960877983	0.0
4	0.0	0.0	0.0	4.869907243450152	0.0
5	0.0	0.0	0.0	7.981885723327783	0.0
6	0.0	0.0	0.0	10.723892911772019	0.0
7	0.0	0.0	0.0	13.005508126558816	0.0
8	0.0	0.0	0.0	16.284764868550933	0.0
9	0.0	0.0	0.0	17.939463353251	0.0
10	0.0	0.0	0.0	21.58341684688011	0.0
11	0.0	0.0	0.0	29.261639779072134	0.0
12	0.0	0.0	0.0	35.52251851740221	0.0
13	0.0	0.0	0.0	37.76721195361419	0.0
14	0.0	0.0	0.0	38.66614423605826	0.0
15	0.0	0.0	0.0	39.99834228749256	0.0
16	0.0	0.0	0.0	42.96263365307091	0.0
17	0.0	0.0	0.0	46.31347343515707	0.0
18	0.0	0.0	0.0	49.37270651707067	0.0
19	0.0	0.0	0.0	51.095220513438775	0.0
20	0.0	0.0	0.0	52.58264073602435	0.0
21	0.0	0.0	0.0	54.18308681138999	0.0
22	0.0	0.0	0.0	55.59063543134433	0.0
23	0.0	0.0	0.0	56.60184006088326	0.0
24	0.0	0.0	0.0	57.210672654525176	0.0
25	0.0	0.0	0.0	57.61379819610739	0.0
26	0.0	0.0	0.0	57.91143294176155	0.0
27	0.0	0.0	0.0	58.16536435767408	0.0
28	0.0	0.0	0.0	58.38915554617859	0.0
29	0.0	0.0	0.0	58.609932711942314	0.0
30	0.0	0.0	0.0	58.83447740613203	0.0
31	0.0	0.0	0.0	59.019086299006126	0.0
32	0.0	0.0	0.0	59.22705386812144	0.0
33	0.0	0.0	0.0	59.38981109612472	0.0
34	0.0	0.0	0.0	59.5472937843316	0.0
35	0.0	0.0	0.0	59.704776472538484	0.0
36	0.0	0.0	0.0	59.838146978818955	0.0
37	0.0	0.0	0.0	59.97377800215503	0.0
38	7.535056852003948E-4	0.0	0.0	60.1169440823431	0.0
39	7.535056852003948E-4	0.0	0.0	60.3068275150136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCTAGC	30	2.150964E-6	45.000004	21
TGTTCTG	30	2.150964E-6	45.000004	1
GTCAAGC	30	2.150964E-6	45.000004	16
TGGGACC	120	0.0	45.000004	6
TGGGACA	65	0.0	45.000004	6
TCAAGCG	30	2.150964E-6	45.000004	17
CATGGTC	40	6.7375368E-9	45.0	12
ATCCTAG	45	3.8016879E-10	45.0	14
ATGGGAT	20	7.010727E-4	45.0	5
GAAGCTA	20	7.010727E-4	45.0	7
ACACGAC	35	1.2010969E-7	45.0	26
GACACGA	35	1.2010969E-7	45.0	25
CCCTACA	20	7.010727E-4	45.0	27
GGCACCG	40	6.7375368E-9	45.0	8
GTGGGTG	20	7.010727E-4	45.0	4
TGGGTAG	25	3.8721184E-5	45.0	6
TTGTCGG	20	7.010727E-4	45.0	2
TCGGGGC	25	3.8721184E-5	45.0	5
CGACCAA	35	1.2010969E-7	45.0	29
GTCTAGG	45	3.8016879E-10	45.0	7
>>END_MODULE