##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549553_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	50573
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.013742510825935	33.0	31.0	34.0	30.0	34.0
2	32.216755976509205	34.0	31.0	34.0	30.0	34.0
3	32.309928222569354	34.0	31.0	34.0	30.0	34.0
4	35.85090858758626	37.0	35.0	37.0	35.0	37.0
5	35.918988393016036	37.0	35.0	37.0	35.0	37.0
6	35.89935340992229	37.0	35.0	37.0	35.0	37.0
7	35.85646491210725	37.0	35.0	37.0	35.0	37.0
8	35.70041326399462	37.0	35.0	37.0	35.0	37.0
9	37.52846380479703	39.0	37.0	39.0	35.0	39.0
10	37.11573369189093	39.0	37.0	39.0	33.0	39.0
11	37.1175132976094	39.0	37.0	39.0	34.0	39.0
12	37.10436398868962	39.0	37.0	39.0	34.0	39.0
13	37.057125343562774	39.0	37.0	39.0	33.0	39.0
14	38.240444505961676	40.0	38.0	41.0	33.0	41.0
15	38.23629209261859	40.0	38.0	41.0	33.0	41.0
16	38.36104245348309	40.0	38.0	41.0	34.0	41.0
17	38.339212623336564	40.0	38.0	41.0	34.0	41.0
18	38.12997053763866	39.0	37.0	41.0	34.0	41.0
19	37.83467462875447	39.0	37.0	41.0	34.0	41.0
20	37.38674786941649	39.0	35.0	41.0	34.0	41.0
21	37.28883000810709	39.0	35.0	40.0	33.0	41.0
22	37.26306527198307	39.0	35.0	40.0	33.0	41.0
23	37.14058885175884	39.0	35.0	40.0	33.0	41.0
24	37.00569473829909	38.0	35.0	40.0	33.0	41.0
25	36.94133233148123	38.0	35.0	40.0	33.0	41.0
26	36.87495303818243	38.0	35.0	40.0	33.0	41.0
27	36.77529511794831	38.0	35.0	40.0	33.0	41.0
28	36.73770589049493	38.0	35.0	40.0	33.0	41.0
29	36.56492594862871	38.0	35.0	40.0	32.0	41.0
30	36.30969094180689	38.0	35.0	40.0	31.0	41.0
31	36.02748502165187	38.0	35.0	40.0	30.0	41.0
32	35.718387281751134	38.0	35.0	40.0	30.0	41.0
33	35.22699859608882	38.0	35.0	40.0	25.0	41.0
34	34.87277796452653	38.0	35.0	40.0	22.0	41.0
35	34.59881755086706	38.0	34.0	40.0	21.0	41.0
36	34.23953492970558	38.0	34.0	40.0	19.0	41.0
37	34.11413204674431	38.0	34.0	40.0	18.0	41.0
38	34.02173096316216	38.0	34.0	40.0	18.0	41.0
39	34.04053546358729	38.0	34.0	40.0	18.0	41.0
40	33.894667114863665	38.0	33.0	40.0	18.0	41.0
41	33.692088663911576	37.0	33.0	40.0	16.0	41.0
42	33.37939216578016	37.0	33.0	40.0	15.0	41.0
43	33.307931109485295	37.0	33.0	40.0	15.0	41.0
44	33.24914480058529	37.0	33.0	40.0	15.0	41.0
45	33.22941095050719	37.0	33.0	40.0	15.0	41.0
46	32.92598817550867	37.0	32.0	40.0	13.0	41.0
47	32.763648587190794	36.0	32.0	40.0	12.0	41.0
48	32.68275562058806	36.0	32.0	40.0	12.0	41.0
49	32.68319063531924	36.0	31.0	40.0	12.0	41.0
50	32.52304589405414	36.0	31.0	40.0	10.0	41.0
51	31.56081308207937	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	2.0
12	2.0
13	1.0
14	6.0
15	7.0
16	5.0
17	12.0
18	18.0
19	27.0
20	75.0
21	104.0
22	181.0
23	260.0
24	458.0
25	732.0
26	1188.0
27	1477.0
28	1434.0
29	1377.0
30	1261.0
31	1462.0
32	1570.0
33	1984.0
34	3108.0
35	4055.0
36	4863.0
37	6077.0
38	9612.0
39	9206.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.03403001601645	5.160856583552489	39.92446562394954	31.880647776481524
2	35.85114586834872	4.777252684238626	41.666501888359406	17.70509955905325
3	18.96268760010282	5.2933383425938745	56.6745101140925	19.069463943210803
4	15.130603286338559	5.619599390979376	57.508947462084514	21.740849860597553
5	13.230379846953907	6.592450517074329	58.15553753979397	22.0216320961778
6	17.829671959345898	7.5672789828564655	61.3766238902181	13.226425167579539
7	65.29966582959287	3.1459474423111145	26.569513376702986	4.984873351393036
8	64.53839005002669	3.8044015581436734	25.857671089316437	5.799537302513199
9	59.822434896090805	5.716489035651435	28.26804816799478	6.193027900262986
10	29.46433867874162	21.72305380341289	34.097245565815754	14.71536195202974
11	21.058667668518773	18.877661993553872	39.426176022778954	20.6374943151484
12	19.656733830304706	15.662507662191288	45.010183299389	19.670575208115
13	20.41010025112214	16.860775512625313	46.22822454669488	16.50089968955767
14	17.675439463745477	19.6448697921816	45.38785517964131	17.29183556443161
15	14.456330453008523	18.43276056393728	47.11011804717932	20.000790935874875
16	14.98032547011251	17.754533051232873	45.8327566092579	21.432384869396714
17	13.975836909022602	18.013564550254088	43.56277064836968	24.447827892353626
18	15.601210131888557	17.760465070294426	45.057639451881435	21.580685345935578
19	16.243845530223638	20.524785952978863	44.699740968500976	18.531627548296523
20	17.70509955905325	22.15015917584482	42.338797382002255	17.80594388309968
21	16.78168192513792	22.822454669487673	42.69076384632116	17.70509955905325
22	15.168172740395072	21.30978980879125	41.23741917624029	22.28461827457339
23	15.3342692741186	21.839716844956794	39.748482391790084	23.07753148913452
24	15.755442627488977	21.438316888458267	40.462302018863824	22.343938465188934
25	16.097522393371957	22.251003499891247	38.1923160579756	23.459158048761196
26	15.462796353785615	23.429497953453424	39.27787554624009	21.82983014652087
27	16.281414984280147	22.148181836157633	41.87016787613944	19.700235303422776
28	15.78905740217112	20.924208569790203	41.061435944080834	22.225298083957842
29	16.874616890435608	19.85644513871038	40.34959365669428	22.91934431415973
30	21.442271567832638	20.485239159235167	39.31346766060941	18.759021612322783
31	21.208945484744824	24.348960907994385	34.90795483756155	19.534138769699247
32	19.95728946275681	24.040495916793546	34.107132264251675	21.89508235619797
33	21.12194253850869	22.87386550135448	32.837680185078995	23.166511775057838
34	22.047337512111206	21.266288335673185	33.14416783659265	23.54220631562296
35	22.64251675795385	21.901014375259525	32.99982203942815	22.456646827358473
36	23.50068218219208	24.950072172898583	33.9212623336563	17.62798331125304
37	23.967334348367704	23.85462598619817	33.81448599054831	18.363553674885807
38	21.293971091293773	24.392462381112452	32.078381745200005	22.23518478239377
39	21.734917841535996	25.51559132343345	30.393688331718504	22.355802503312045
40	24.698950032626104	23.605481185612874	32.77638265477626	18.919186126984755
41	21.85751290214146	23.354359045340402	35.339014889367846	19.449113163150297
42	21.685484349356376	25.337630751586815	34.554011033555454	18.422873865501355
43	20.497103197358275	23.534296956874222	35.4101991181065	20.558400727661006
44	20.67506376920491	22.46851086548158	33.219306744705676	23.637118620607833
45	24.070156012101318	20.06802048523916	31.449587724675222	24.4122357779843
46	24.137385561465603	21.493682399699445	34.65683269728907	19.712099341545883
47	19.6448697921816	24.99752832539102	36.21497637079074	19.142625511636645
48	18.842069879184546	23.69643881122338	37.01579894410061	20.445692365491468
49	21.51741047594566	21.519387815632847	36.61637632728926	20.346825381132223
50	21.104146481324026	18.85591125699484	35.206533130326456	24.833409131354674
51	18.76297629169715	18.83020584106144	34.05769877207205	28.34911909516936
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	154.0
1	300.5
2	447.0
3	2009.0
4	3571.0
5	2225.5
6	880.0
7	838.5
8	797.0
9	787.0
10	777.0
11	734.5
12	692.0
13	638.0
14	584.0
15	539.5
16	495.0
17	478.0
18	461.0
19	416.0
20	371.0
21	343.0
22	315.0
23	324.0
24	333.0
25	311.5
26	303.0
27	316.0
28	317.0
29	318.0
30	395.0
31	472.0
32	495.0
33	518.0
34	631.0
35	744.0
36	824.0
37	904.0
38	979.0
39	1054.0
40	1234.5
41	1415.0
42	1612.0
43	1809.0
44	2179.0
45	2549.0
46	3163.0
47	3777.0
48	4689.0
49	5601.0
50	5619.0
51	5637.0
52	4814.0
53	3991.0
54	3197.5
55	2404.0
56	2118.5
57	1833.0
58	1705.5
59	1578.0
60	1515.0
61	1452.0
62	1319.5
63	1187.0
64	1039.5
65	892.0
66	751.0
67	610.0
68	523.0
69	436.0
70	375.0
71	314.0
72	262.5
73	211.0
74	194.5
75	137.0
76	96.0
77	78.5
78	61.0
79	49.0
80	37.0
81	21.0
82	5.0
83	4.0
84	3.0
85	3.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	50573.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	68.52866153876575
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.28228063594656	55.70165898799755
2	11.616700810803012	15.921539161212506
3	3.950139942868684	8.120934095268225
4	1.3994286868453703	3.836038993138631
5	0.6290215540871974	2.155300259031499
6	0.29431283723345936	1.2101318885571353
7	0.21640649796577893	1.0381033357720524
8	0.12118763886083621	0.6643861348941135
9	0.08079175924055745	0.49828960117058513
>10	0.37799001644689384	4.334328594309216
>50	0.017312519837262313	0.9372590117256243
>100	0.011541679891508208	1.2912028157317146
>500	0.0	0.0
>1k	0.002885419972877052	4.29082712119115
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2170	4.29082712119115	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCC	186	0.36778518181638425	No Hit
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	180	0.3559211436932751	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	175	0.3460344452573508	TruSeq Adapter, Index 15 (95% over 23bp)
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	112	0.22146204496470448	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	95	0.18784727028256185	TruSeq Adapter, Index 15 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	95	0.18784727028256185	TruSeq Adapter, Index 15 (95% over 23bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	89	0.17598323215945266	No Hit
CGTTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	88	0.1740058924722678	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTG	54	0.10677634310798252	No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	53	0.10479900342079766	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003954679374369723	0.0	0.0	0.3796492199394934	0.0
2	0.003954679374369723	0.0	0.0	1.1666304154390683	0.0
3	0.003954679374369723	0.0	0.0	1.6945801119174262	0.0
4	0.003954679374369723	0.0	0.0	2.4894706661657406	0.0
5	0.003954679374369723	0.0	0.0	4.134617285903546	0.0
6	0.003954679374369723	0.0	0.0	5.566211219425385	0.0
7	0.003954679374369723	0.0	0.0	6.894983489213612	0.0
8	0.003954679374369723	0.0	0.0	8.767524172977676	0.0
9	0.003954679374369723	0.0	0.0	9.63557629565183	0.0
10	0.003954679374369723	0.0	0.0	11.4942756016056	0.0
11	0.003954679374369723	0.0	0.0	15.994700729638344	0.0
12	0.003954679374369723	0.0	0.0	19.8406264212129	0.0
13	0.003954679374369723	0.0	0.0	21.157534652878017	0.0
14	0.003954679374369723	0.0	0.0	21.63802819686394	0.0
15	0.003954679374369723	0.0	0.0	22.205524687085994	0.0
16	0.0059320190615545845	0.0	0.0	23.74191762402863	0.0
17	0.0059320190615545845	0.0	0.0	25.527455361556562	0.0
18	0.0059320190615545845	0.0	0.0	27.225990152848357	0.0
19	0.0059320190615545845	0.0	0.0	28.281889545805075	0.0
20	0.0059320190615545845	0.0	0.0	29.211239198781957	0.0
21	0.0059320190615545845	0.0	0.0	30.259229232989934	0.0
22	0.0059320190615545845	0.0	0.0	31.21626164158741	0.0
23	0.0059320190615545845	0.0	0.0	31.941945306784252	0.0
24	0.0059320190615545845	0.0	0.0	32.373005358590554	0.0
25	0.0059320190615545845	0.0	0.0	32.6794930101042	0.0
26	0.0059320190615545845	0.0	0.0	32.93259249006387	0.0
27	0.0059320190615545845	0.0	0.0	33.130326458782356	0.0
28	0.0059320190615545845	0.0	0.0	33.31224171000336	0.0
29	0.0059320190615545845	0.0	0.0	33.46845154529097	0.0
30	0.0059320190615545845	0.0	0.0	33.6464121171376	0.0
31	0.0059320190615545845	0.0	0.0	33.82832736835861	0.0
32	0.0059320190615545845	0.0	0.0	33.97662784489747	0.0
33	0.0059320190615545845	0.0	0.0	34.1170189626876	0.0
34	0.0059320190615545845	0.0	0.0	34.23763668360588	0.0
35	0.0059320190615545845	0.0	0.0	34.38000514108319	0.0
36	0.0059320190615545845	0.0	0.0	34.516441579498945	0.0
37	0.0059320190615545845	0.0	0.0	34.62717260198129	0.0
38	0.0059320190615545845	0.0	0.0	34.76558638008424	0.0
39	0.0059320190615545845	0.0	0.0	34.90004547881281	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	20	6.969406E-4	45.000004	10
TTCGTTG	20	6.969406E-4	45.000004	1
GGCGCGA	20	6.969406E-4	45.000004	8
GCGCGAC	20	6.969406E-4	45.000004	9
ACCCGCT	20	6.969406E-4	45.000004	34
TCTGCGG	20	6.969406E-4	45.000004	2
CTGCGGG	20	6.969406E-4	45.000004	3
TCAGATC	20	6.969406E-4	45.000004	17
CCCTGGG	20	6.969406E-4	45.000004	3
TTAAGCA	20	6.969406E-4	45.000004	45
CAGACGT	20	6.969406E-4	45.000004	23
GTGGCGA	20	6.969406E-4	45.000004	28
CTATGGG	20	6.969406E-4	45.000004	3
TAGGGGG	20	6.969406E-4	45.000004	5
GACCTCA	20	6.969406E-4	45.000004	13
CGACCTC	20	6.969406E-4	45.000004	12
TTCGGGC	20	6.969406E-4	45.000004	4
TGGCCTG	20	6.969406E-4	45.000004	1
ACGTGGC	20	6.969406E-4	45.000004	26
AGGGAAT	25	3.837957E-5	45.0	6
>>END_MODULE