FastQCFastQC Report
Sat 18 Jun 2016
SRR3549552_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549552_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731685
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279473.819539829298127No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC37960.518802490142616No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG22890.31283954160601896No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC21920.2995824705986866No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT10050.13735418930277374No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC9920.13557746844612095No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG302.1644155E-645.00000424
AACCGTA207.0316053E-445.040
TCGATAA207.0316053E-445.022
CAATCGA253.889411E-545.041
CGTTTTT131100.043.815791
CCGATGA5700.043.02631818
CGATGAA6000.042.019
ATCGAAT603.6379788E-1241.25000443
CCAATCG501.0804797E-940.540
TCAAGCG3300.040.22727617
CGAATAT900.040.014
CACGACC3400.039.0441227
GCGGGAT2550.038.823535
GTTTTTT154650.038.4675062
ATGAATG6700.038.28358521
AACACGT2600.038.07692741
ACGACCA3500.037.9285728
GCTACGA950.037.89473710
GATGAAT6900.037.82608820
TTGGGAC9950.037.763825