##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549552_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	731685
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.33952178874789	33.0	31.0	34.0	31.0	34.0
2	32.48559831074848	34.0	31.0	34.0	31.0	34.0
3	32.56999665156454	34.0	31.0	34.0	31.0	34.0
4	36.095215837416376	37.0	35.0	37.0	35.0	37.0
5	36.070921229764174	37.0	35.0	37.0	35.0	37.0
6	36.008401156235266	37.0	35.0	37.0	35.0	37.0
7	36.07061918721854	37.0	35.0	37.0	35.0	37.0
8	35.88114694164839	37.0	35.0	37.0	35.0	37.0
9	37.741748156652115	39.0	38.0	39.0	35.0	39.0
10	37.328358514934706	39.0	37.0	39.0	34.0	39.0
11	37.22495062766081	39.0	37.0	39.0	34.0	39.0
12	37.11969905082105	39.0	37.0	39.0	34.0	39.0
13	37.06275241394862	39.0	37.0	39.0	33.0	39.0
14	38.26679923737674	40.0	38.0	41.0	33.0	41.0
15	38.34539590124165	40.0	38.0	41.0	33.0	41.0
16	38.40878520128197	40.0	38.0	41.0	34.0	41.0
17	38.29833876599903	40.0	38.0	41.0	33.0	41.0
18	37.978852921680776	39.0	37.0	41.0	33.0	41.0
19	37.65142650184164	39.0	37.0	41.0	34.0	41.0
20	37.19314869103508	39.0	35.0	41.0	33.0	41.0
21	37.1081954666284	38.0	35.0	41.0	33.0	41.0
22	37.11974005207159	38.0	35.0	41.0	33.0	41.0
23	36.9838195398293	38.0	35.0	40.0	33.0	41.0
24	36.91154936892242	38.0	35.0	40.0	33.0	41.0
25	36.842146552136505	38.0	35.0	40.0	33.0	41.0
26	36.75133834915298	38.0	35.0	40.0	33.0	41.0
27	36.64984248686252	38.0	35.0	40.0	32.0	41.0
28	36.46051237896089	38.0	35.0	40.0	32.0	41.0
29	36.3314185749332	38.0	35.0	40.0	31.0	41.0
30	36.14644553325543	38.0	35.0	40.0	31.0	41.0
31	35.81869930366209	38.0	35.0	40.0	30.0	41.0
32	35.29652514401689	38.0	35.0	40.0	26.0	41.0
33	34.766873722981884	38.0	34.0	40.0	22.0	41.0
34	34.228208860370245	38.0	34.0	40.0	18.0	41.0
35	33.856088344027825	38.0	33.0	40.0	16.0	41.0
36	33.62776331344773	38.0	33.0	40.0	15.0	41.0
37	33.46543799585887	37.0	33.0	40.0	15.0	41.0
38	33.3259831758202	37.0	33.0	40.0	12.0	41.0
39	33.267324053383625	37.0	33.0	40.0	12.0	41.0
40	33.11189241271859	37.0	33.0	40.0	11.0	41.0
41	32.91904713093749	37.0	33.0	40.0	10.0	41.0
42	32.78766682383813	37.0	32.0	40.0	10.0	41.0
43	32.64623437681516	36.0	32.0	40.0	10.0	41.0
44	32.552770659505114	36.0	32.0	40.0	10.0	41.0
45	32.51250606476831	36.0	32.0	40.0	10.0	41.0
46	32.34996890738501	35.0	31.0	40.0	10.0	41.0
47	32.18423911929314	35.0	31.0	40.0	10.0	41.0
48	32.109769914649064	35.0	31.0	40.0	10.0	41.0
49	32.096693249144096	35.0	31.0	40.0	10.0	41.0
50	31.99740872096599	35.0	31.0	40.0	10.0	41.0
51	31.016466102216118	35.0	28.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	5.0
11	12.0
12	13.0
13	13.0
14	22.0
15	43.0
16	80.0
17	141.0
18	309.0
19	628.0
20	1221.0
21	2102.0
22	3356.0
23	4991.0
24	7761.0
25	13475.0
26	20946.0
27	24514.0
28	23377.0
29	20597.0
30	19145.0
31	19950.0
32	22598.0
33	29340.0
34	46579.0
35	53562.0
36	65307.0
37	81618.0
38	131118.0
39	138820.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.20528642790272	4.234882497249499	34.40715608492726	34.15267498992053
2	37.785795800105234	7.383641867743633	40.76139322249329	14.069169109657844
3	19.595180986353416	7.555027094993064	57.09656477855908	15.753227140094438
4	15.452141290309354	4.940240677340659	60.24942427410702	19.358193758242958
5	16.477035882927762	5.709150795765938	57.326445123242934	20.487368198063376
6	18.22696925589564	8.474275132058194	59.558006519198834	13.740749092847333
7	66.68662060859522	2.7062192063524604	26.948481928698826	3.6586782563534856
8	67.16838530241839	4.727307516212577	24.321941819225486	3.782365362143545
9	62.259168904651595	4.919466710401334	25.64163540321313	7.179728981733943
10	33.53642619433226	20.805401231404225	31.52012136370159	14.138051210561922
11	26.261300969679574	19.26689764037803	37.79126263351032	16.68053875643207
12	21.09364002268736	18.366920191065827	41.81772210719094	18.721717679055878
13	21.043891838701082	17.756411570552903	44.34490251952685	16.854794071219175
14	18.376760491194982	20.021457321114962	44.019762602759386	17.582019584930674
15	16.888551767495574	17.947614068895767	45.964451915783435	19.199382247825227
16	19.41313543396407	18.305964998599126	42.60617615503939	19.67472341239741
17	18.945857848664385	19.454273355337335	42.30372359690303	19.29614519909524
18	20.102366455510225	17.754088166355743	42.354838489240585	19.788706888893444
19	19.90036696119232	19.998359949978475	40.31543628747343	19.785836801355774
20	22.298803446838463	21.34484101765104	39.52629888544934	16.83005665006116
21	21.164435515283216	22.918879025810288	39.46384031379624	16.45284514511026
22	20.455113880973368	19.485024293240944	40.42313290555362	19.636728920232066
23	19.36516397083444	21.81457867798301	39.51837197701197	19.30188537417058
24	19.32375270779092	20.98279997539925	40.19065581500236	19.502791501807472
25	19.686477104218344	22.860110566705618	37.96223784825437	19.491174480821666
26	18.77761605062288	20.931958424731956	39.74346884246636	20.54695668217881
27	20.128607255854636	20.21060975693092	39.35710039156194	20.3036825956525
28	17.84962108010961	22.16281596588696	39.75850263433035	20.229060319673085
29	20.778203735213925	20.224686852949013	39.053281125074314	19.943828286762745
30	22.085870285710378	20.583311124322627	39.10097924653368	18.22983934343331
31	22.364542118534615	21.116190710483338	36.771971545132125	19.747295625849922
32	22.83851657475553	21.210903599226445	36.03449571878609	19.91608410723194
33	23.378093031837473	20.931001728886063	34.75853680203913	20.932368437237336
34	22.726583160786404	22.441351127876068	34.99579737181984	19.836268339517687
35	22.800932095095565	24.18089751737428	33.0722920382405	19.945878349289654
36	23.181560370924647	26.511955281302747	31.792643008945102	18.5138413388275
37	22.585675529770324	25.56742313973909	32.39850482106371	19.44839650942687
38	21.437230502196982	25.684413374607924	32.59804424034933	20.280311882845762
39	21.964369913282354	24.865618401361242	31.694786690994075	21.47522499436233
40	23.139465753705487	24.951447685821083	32.55977640651373	19.349310153959696
41	21.32133363400917	26.79158380997287	31.029063053089782	20.858019502928173
42	21.54643049946357	26.538606094152538	31.659935628036656	20.25502777834724
43	21.256278316488654	24.619064214791884	32.8038705180508	21.320786950668662
44	22.0663263562872	23.711843211217943	32.565789923259324	21.656040509235535
45	22.125641498732378	22.874050991888588	32.21140244777466	22.78890506160438
46	22.56476489199587	24.32672529845494	32.128989934192994	20.979519875356196
47	20.29233891633695	25.72432125846505	34.20009976970964	19.78324005548836
48	20.491604993952315	24.84142766354374	33.90557412001066	20.761393222493286
49	21.173182448731353	23.688745840081456	34.057688759507165	21.080382951680026
50	20.34864730040933	23.7539378284371	33.671866991943254	22.225547879210318
51	20.249287603271902	22.997191414338136	32.73129830459829	24.02222267779167
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	710.0
1	1717.5
2	2725.0
3	24478.0
4	46231.0
5	29672.0
6	13113.0
7	12676.0
8	12239.0
9	11996.0
10	11753.0
11	11458.5
12	11164.0
13	10617.5
14	10071.0
15	9481.0
16	8891.0
17	8324.5
18	7758.0
19	7324.0
20	6890.0
21	6546.0
22	6202.0
23	6136.0
24	6070.0
25	6067.5
26	6315.5
27	6566.0
28	7032.5
29	7499.0
30	8371.5
31	9244.0
32	10101.0
33	10958.0
34	12457.5
35	13957.0
36	15220.0
37	16483.0
38	16948.0
39	17413.0
40	19505.5
41	21598.0
42	23684.0
43	25770.0
44	28676.0
45	31582.0
46	41329.0
47	51076.0
48	50205.5
49	49335.0
50	50468.0
51	51601.0
52	49420.5
53	47240.0
54	43557.0
55	39874.0
56	37448.0
57	35022.0
58	33668.0
59	32314.0
60	30180.5
61	28047.0
62	25360.5
63	22674.0
64	20858.5
65	19043.0
66	16402.5
67	13762.0
68	11716.0
69	9670.0
70	8204.5
71	6739.0
72	6032.0
73	5325.0
74	4534.5
75	3165.5
76	2587.0
77	2054.0
78	1521.0
79	1077.0
80	633.0
81	474.0
82	315.0
83	209.5
84	104.0
85	85.0
86	66.0
87	47.5
88	29.0
89	18.0
90	7.0
91	5.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	731685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.61607690341661
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.03930071221355	28.2049125327461
2	8.322235076705665	6.427441394607544
3	4.107426484462389	4.758380909973892
4	2.6614805962347483	4.111037575246086
5	2.040950378943321	3.940674839466628
6	1.6193610420852607	3.752002232133677
7	1.3138126106366665	3.551400216631684
8	1.1181341221407168	3.4542362599136114
9	0.8570067211664762	2.9784813706178612
>10	4.808007304566129	28.680791622352675
>50	0.07934331302074212	2.071296971845405
>100	0.027628470433332785	2.0648679212822554
>500	0.003542111594016785	0.9122021838255736
>1k	0.001416844637606714	1.269614735382962
>5k	0.0	0.0
>10k+	3.542111594016785E-4	3.8226592339741052
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27947	3.819539829298127	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	3796	0.518802490142616	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	2289	0.31283954160601896	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	2192	0.2995824705986866	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	1005	0.13735418930277374	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC	992	0.13557746844612095	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.055898371566999464	0.0
2	0.0	0.0	0.0	0.25338772832571393	0.0
3	0.0	0.0	0.0	0.38377170503700364	0.0
4	0.0	0.0	0.0	0.7031714467291252	0.0
5	0.0	0.0	0.0	1.6867914471391376	0.0
6	0.0	0.0	0.0	2.5221235914362055	0.0
7	0.0	0.0	0.0	3.2688930345708878	0.0
8	0.0	0.0	0.0	4.3174316816662905	0.0
9	0.0	0.0	0.0	5.051354066299022	0.0
10	0.0	0.0	0.0	6.3494536583365795	0.0
11	0.0	0.0	0.0	7.7638601310673305	0.0
12	0.0	0.0	0.0	9.224324675235929	0.0
13	0.0	0.0	0.0	9.691602260535612	0.0
14	0.0	0.0	0.0	9.93583304290781	0.0
15	0.0	0.0	0.0	10.206441296459541	0.0
16	0.0	0.0	0.0	10.60770686839282	0.0
17	0.0	0.0	0.0	11.045873565810425	0.0
18	0.0	0.0	0.0	11.468323117188408	0.0
19	0.0	0.0	0.0	11.883529114304652	0.0
20	1.3667083512713805E-4	0.0	0.0	12.171357893082405	0.0
21	1.3667083512713805E-4	0.0	0.0	12.496224468179612	0.0
22	1.3667083512713805E-4	0.0	0.0	12.819861005760675	0.0
23	1.3667083512713805E-4	0.0	0.0	13.075708809118678	0.0
24	1.3667083512713805E-4	0.0	0.0	13.25994109487006	0.0
25	2.733416702542761E-4	0.0	0.0	13.422716059506481	0.0
26	2.733416702542761E-4	0.0	0.0	13.579067494891927	0.0
27	2.733416702542761E-4	0.0	0.0	13.745669242911909	0.0
28	4.1001250538141414E-4	0.0	0.0	13.895460478211252	0.0
29	4.1001250538141414E-4	0.0	0.0	14.049351838564409	0.0
30	4.1001250538141414E-4	0.0	0.0	14.243151082774691	0.0
31	4.1001250538141414E-4	0.0	0.0	14.397589126468358	0.0
32	4.1001250538141414E-4	0.0	0.0	14.66245720494475	0.0
33	4.1001250538141414E-4	0.0	0.0	14.826872219602699	0.0
34	4.1001250538141414E-4	0.0	0.0	15.004544305267977	0.0
35	4.1001250538141414E-4	0.0	0.0	15.192740045238047	0.0
36	4.1001250538141414E-4	0.0	0.0	15.366175335014384	0.0
37	5.466833405085522E-4	0.0	0.0	15.531820387188477	0.0
38	5.466833405085522E-4	0.0	0.0	15.768670944463807	0.0
39	5.466833405085522E-4	0.0	0.0	16.127978570013052	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	30	2.1644155E-6	45.000004	24
AACCGTA	20	7.0316053E-4	45.0	40
TCGATAA	20	7.0316053E-4	45.0	22
CAATCGA	25	3.889411E-5	45.0	41
CGTTTTT	13110	0.0	43.81579	1
CCGATGA	570	0.0	43.026318	18
CGATGAA	600	0.0	42.0	19
ATCGAAT	60	3.6379788E-12	41.250004	43
CCAATCG	50	1.0804797E-9	40.5	40
TCAAGCG	330	0.0	40.227276	17
CGAATAT	90	0.0	40.0	14
CACGACC	340	0.0	39.04412	27
GCGGGAT	255	0.0	38.82353	5
GTTTTTT	15465	0.0	38.467506	2
ATGAATG	670	0.0	38.283585	21
AACACGT	260	0.0	38.076927	41
ACGACCA	350	0.0	37.92857	28
GCTACGA	95	0.0	37.894737	10
GATGAAT	690	0.0	37.826088	20
TTGGGAC	995	0.0	37.76382	5
>>END_MODULE