FastQCFastQC Report
Sat 18 Jun 2016
SRR3549551_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549551_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences743055
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279033.755172901063851No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC40220.5412789093674089No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC22980.30926378262712717No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG22520.3030731237929898No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT11380.15315151637496552No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC9630.1296000968972687No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATCCG207.031676E-445.017
TATCGCG207.031676E-445.01
ACCCGAG650.044.99999641
CGATGAA4850.043.6082519
CGTTTTT132450.043.3691981
CCGATGA4650.042.58064718
ATCGAAT850.042.3529443
AACCCGA700.041.78571324
CGAATAT650.041.5384614
TTACACG6450.040.11627634
CAATCGA403.4560253E-739.37541
TATGCGG750.039.02
ACACGCG6600.038.86363636
AACGACG700.038.57142611
TACACGC6850.037.77372435
GTTTTTT158700.037.6984862
AAACACG3050.037.6229540
GATGAAT6000.037.520
CGAACGA301.13953356E-437.49999622
ATTCGAC301.13953356E-437.49999611