##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549551_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	743055
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25742643545902	33.0	31.0	34.0	30.0	34.0
2	32.424768018518144	34.0	31.0	34.0	30.0	34.0
3	32.513419598818395	34.0	31.0	34.0	31.0	34.0
4	36.01722348951289	37.0	35.0	37.0	35.0	37.0
5	36.04313274252915	37.0	35.0	37.0	35.0	37.0
6	35.99371244389715	37.0	35.0	37.0	35.0	37.0
7	35.995136295429006	37.0	35.0	37.0	35.0	37.0
8	35.83869700089495	37.0	35.0	37.0	35.0	37.0
9	37.70621959343521	39.0	38.0	39.0	35.0	39.0
10	37.33066327526226	39.0	37.0	39.0	34.0	39.0
11	37.22754843181191	39.0	37.0	39.0	34.0	39.0
12	37.09869390556554	39.0	37.0	39.0	34.0	39.0
13	37.05203383329632	39.0	37.0	39.0	33.0	39.0
14	38.274256952715476	40.0	38.0	41.0	33.0	41.0
15	38.335229559050134	40.0	38.0	41.0	33.0	41.0
16	38.451631440472106	40.0	38.0	41.0	34.0	41.0
17	38.37092005302434	40.0	38.0	41.0	34.0	41.0
18	38.10283760959821	39.0	38.0	41.0	34.0	41.0
19	37.799982504659816	39.0	37.0	41.0	34.0	41.0
20	37.29666713769505	39.0	35.0	41.0	33.0	41.0
21	37.24562111822139	39.0	35.0	41.0	33.0	41.0
22	37.238614907375634	39.0	35.0	41.0	33.0	41.0
23	37.052220898856746	39.0	35.0	41.0	33.0	41.0
24	36.97862607747744	38.0	35.0	41.0	33.0	41.0
25	36.912655187031916	38.0	35.0	40.0	33.0	41.0
26	36.84183943315098	38.0	35.0	40.0	33.0	41.0
27	36.75010194400145	38.0	35.0	40.0	33.0	41.0
28	36.63474843719509	38.0	35.0	40.0	32.0	41.0
29	36.4511092718574	38.0	35.0	40.0	32.0	41.0
30	36.168936350606614	38.0	35.0	40.0	31.0	41.0
31	35.832114715599786	38.0	35.0	40.0	30.0	41.0
32	35.38794167322742	38.0	35.0	40.0	27.0	41.0
33	34.8096049417607	38.0	35.0	40.0	22.0	41.0
34	34.28624798971812	38.0	34.0	40.0	18.0	41.0
35	33.98097449044822	38.0	34.0	40.0	16.0	41.0
36	33.73942978648956	38.0	33.0	40.0	15.0	41.0
37	33.60065674815458	38.0	33.0	40.0	15.0	41.0
38	33.4545935361447	37.0	33.0	40.0	14.0	41.0
39	33.40934520324875	37.0	33.0	40.0	12.0	41.0
40	33.25548849008485	37.0	33.0	40.0	12.0	41.0
41	33.08972014184683	37.0	33.0	40.0	11.0	41.0
42	32.899987214943714	37.0	33.0	40.0	10.0	41.0
43	32.785190867432426	36.0	32.0	40.0	10.0	41.0
44	32.6828942675845	36.0	32.0	40.0	10.0	41.0
45	32.622103343628666	36.0	32.0	40.0	10.0	41.0
46	32.44338575206412	35.0	31.0	40.0	10.0	41.0
47	32.33880937481075	35.0	31.0	40.0	10.0	41.0
48	32.23033691987807	35.0	31.0	40.0	10.0	41.0
49	32.21435559951821	35.0	31.0	40.0	10.0	41.0
50	32.047250876449255	35.0	31.0	40.0	10.0	41.0
51	31.198506167107414	35.0	29.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	8.0
10	12.0
11	14.0
12	18.0
13	20.0
14	30.0
15	38.0
16	84.0
17	163.0
18	324.0
19	644.0
20	1244.0
21	2145.0
22	3365.0
23	5004.0
24	7915.0
25	13450.0
26	20681.0
27	24615.0
28	23537.0
29	20539.0
30	19283.0
31	19919.0
32	22491.0
33	28796.0
34	46752.0
35	53914.0
36	65379.0
37	80305.0
38	131597.0
39	150709.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.322069025846	4.344496706165762	34.22613400084785	34.10730026714039
2	37.83232735127279	7.500790654796752	40.45609006062808	14.21079193330238
3	19.68050817234256	7.638061785466756	56.82015463189132	15.861275410299372
4	15.55012751411403	4.874470934183877	60.23955158097315	19.33584997072895
5	16.662696570240428	5.7850361009615705	57.11596046053119	20.43630686826682
6	18.282495912146477	8.480933443688556	59.363707935482566	13.872862708682398
7	66.55725350075028	2.744480556620977	26.937979019049735	3.7602869235790086
8	67.04927629852435	4.755906359556157	24.315831264172907	3.8789860777466005
9	62.16767264872721	4.959390623843457	25.65637806084341	7.216558666585919
10	33.5595615398591	20.79173143307023	31.493765602815404	14.154941424255272
11	26.30357106809052	19.28740133637483	37.688192664069284	16.72083493146537
12	21.23544017603004	18.326772580764548	41.78667797134802	18.6511092718574
13	21.078385852998768	17.804469386519166	44.27478450451178	16.842360255970284
14	18.505090471095677	20.053293497789532	44.0001076636319	17.441508367482893
15	16.947332297070876	17.96650315252572	45.80764546366016	19.27851908674324
16	19.42332667164611	18.27966974180915	42.65767675340318	19.639326833141556
17	19.003303927703872	19.52722207642772	42.1133025146187	19.356171481249707
18	20.1760300381533	17.729104844190537	42.2442484069147	19.850616710741466
19	20.016418703864453	19.961779410676193	40.182220696987436	19.839581188471918
20	22.495373828316882	21.26437477710264	39.4527995908782	16.787451803702282
21	21.194797154988525	22.927777889927395	39.381741593825495	16.495683361258585
22	20.450168560873692	19.44405192078648	40.390011506550664	19.715768011789166
23	19.589397823848838	21.76353029048994	39.47002577198189	19.17704611367934
24	19.528029553666958	20.91769788239094	40.02731964659413	19.526952917347977
25	19.724111943261267	22.88686570980614	37.95519847117642	19.43382387575617
26	18.965083338380065	20.937884813371824	39.66597358203632	20.43105826621179
27	20.213577729777743	20.20388800290692	39.18942743134761	20.39310683596773
28	17.934204062956308	22.220831566976873	39.55696415473955	20.288000215327266
29	20.877727759048792	20.248433830604736	38.86630195611362	20.007536454232863
30	22.280046564520795	20.582191089488667	39.018645995249344	18.119116350741198
31	22.50533271426745	21.08417277321329	36.76497702054357	19.645517491975696
32	22.92118349247364	21.28604208302212	35.92075956692304	19.872014857581203
33	23.39974833626044	21.022131605332042	34.7285194231921	20.849600635215428
34	22.7335796138913	22.44261864868684	34.95986165223301	19.86394008518885
35	22.75080579499499	24.336556513313283	33.0079200059215	19.904717685770233
36	23.23327344543809	26.535317035751056	31.818102293908257	18.413307224902596
37	22.66131040097974	25.532968622780277	32.326409216006894	19.47931176023309
38	21.33408697875662	25.782748248783736	32.57295893305341	20.310205839406233
39	22.085579129404955	24.78443722200914	31.698326503421686	21.43165714516422
40	23.098559326025665	24.78309142661041	32.65989731581108	19.458451931552844
41	21.291021525997404	26.817799489943546	30.978998862802886	20.912180121256167
42	21.614416160311148	26.61364232795688	31.705324639495057	20.066616872236914
43	21.28348507176454	24.608676342935585	32.82825632019164	21.279582265108235
44	22.08679034526381	23.710627073366037	32.680084246791964	21.522498334578195
45	22.207642772069363	22.859815222291754	32.22964652683852	22.702895478800357
46	22.583119688313786	24.32605930920322	32.179582938005936	20.911238064477057
47	20.232015126740283	25.787323953139403	34.19141247956073	19.789248440559582
48	20.582729407648156	24.904482171575456	33.82199164261057	20.690796778165815
49	21.236785971428766	23.651412075822112	34.09263109729428	21.019170855454846
50	20.40898722167269	23.755980378303086	33.76492991770461	22.070102482319612
51	20.31666565732012	23.04714994179435	32.73862634663652	23.897558054249014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	677.0
1	1733.5
2	2790.0
3	24746.0
4	46702.0
5	30286.0
6	13870.0
7	13006.5
8	12143.0
9	12053.5
10	11964.0
11	11611.0
12	11258.0
13	10702.5
14	10147.0
15	9545.5
16	8944.0
17	8366.5
18	7789.0
19	7414.5
20	7040.0
21	6620.0
22	6200.0
23	6194.5
24	6189.0
25	6093.5
26	6369.0
27	6740.0
28	7187.0
29	7634.0
30	8574.0
31	9514.0
32	10367.0
33	11220.0
34	12638.5
35	14057.0
36	15181.0
37	16305.0
38	16994.0
39	17683.0
40	19685.5
41	21688.0
42	23755.0
43	25822.0
44	28882.5
45	31943.0
46	42200.5
47	52458.0
48	51077.0
49	49696.0
50	51136.5
51	52577.0
52	50342.5
53	48108.0
54	44431.5
55	40755.0
56	38183.5
57	35612.0
58	34291.5
59	32971.0
60	30762.0
61	28553.0
62	25872.0
63	23191.0
64	21175.0
65	19159.0
66	16604.5
67	14050.0
68	11948.0
69	9846.0
70	8451.5
71	7057.0
72	6240.0
73	5423.0
74	4604.5
75	3258.5
76	2731.0
77	2185.5
78	1640.0
79	1130.5
80	621.0
81	464.5
82	308.0
83	201.5
84	95.0
85	80.0
86	65.0
87	45.0
88	25.0
89	17.0
90	9.0
91	5.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	743055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.618104879596174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.93729159947125	28.1669997662207
2	8.450906035165717	6.527159511872839
3	4.092478568745475	4.741312997559373
4	2.7066673830783117	4.181054594956015
5	2.0474506970399378	3.9534332877045317
6	1.6193566437465508	3.7521890823405095
7	1.2815423693518795	3.4643516339093945
8	1.0762456161449991	3.3250052864394553
9	0.9129618552089386	3.1731171007976537
>10	4.767058318378792	28.611530761414887
>50	0.07355228706325566	1.9547459326536958
>100	0.028857830424578026	2.047569941543401
>500	0.0038711723740281864	0.9896723957465688
>1k	0.0014076990451011588	1.3196537987776962
>5k	0.0	0.0
>10k+	3.519247612752897E-4	3.7922039080632395
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27903	3.755172901063851	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTC	4022	0.5412789093674089	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGC	2298	0.30926378262712717	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCG	2252	0.3030731237929898	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCCGTCTTCT	1138	0.15315151637496552	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTCTGAATCGTATGCC	963	0.1296000968972687	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.6915907974510634E-4	0.0	0.0	0.05840752030468808	0.0
2	2.6915907974510634E-4	0.0	0.0	0.2583927165553021	0.0
3	2.6915907974510634E-4	0.0	0.0	0.38705075667346295	0.0
4	2.6915907974510634E-4	0.0	0.0	0.7210771746371399	0.0
5	2.6915907974510634E-4	0.0	0.0	1.709833054080788	0.0
6	2.6915907974510634E-4	0.0	0.0	2.5485327465665395	0.0
7	2.6915907974510634E-4	0.0	0.0	3.2936996588408665	0.0
8	2.6915907974510634E-4	0.0	0.0	4.3276742636816925	0.0
9	2.6915907974510634E-4	0.0	0.0	5.021431791724704	0.0
10	2.6915907974510634E-4	0.0	0.0	6.275174785177409	0.0
11	2.6915907974510634E-4	0.0	0.0	7.683818829023424	0.0
12	2.6915907974510634E-4	0.0	0.0	9.127722712316046	0.0
13	2.6915907974510634E-4	0.0	0.0	9.577891273189737	0.0
14	2.6915907974510634E-4	0.0	0.0	9.809233502230656	0.0
15	4.0373861961765954E-4	0.0	0.0	10.104366433171165	0.0
16	4.0373861961765954E-4	0.0	0.0	10.498819064537619	0.0
17	4.0373861961765954E-4	0.0	0.0	10.938355841761377	0.0
18	4.0373861961765954E-4	0.0	0.0	11.371029062451635	0.0
19	4.0373861961765954E-4	0.0	0.0	11.761040569002295	0.0
20	4.0373861961765954E-4	0.0	0.0	12.053212750065608	0.0
21	4.0373861961765954E-4	0.0	0.0	12.368801771066744	0.0
22	4.0373861961765954E-4	0.0	0.0	12.688831916883677	0.0
23	4.0373861961765954E-4	0.0	0.0	12.94103397460484	0.0
24	4.0373861961765954E-4	0.0	0.0	13.114776160580307	0.0
25	4.0373861961765954E-4	0.0	0.0	13.265505245237566	0.0
26	4.0373861961765954E-4	0.0	0.0	13.418925920692278	0.0
27	4.0373861961765954E-4	0.0	0.0	13.581767163938066	0.0
28	4.0373861961765954E-4	0.0	0.0	13.734380362153543	0.0
29	4.0373861961765954E-4	0.0	0.0	13.884840287731055	0.0
30	4.0373861961765954E-4	0.0	0.0	14.071367529994415	0.0
31	5.383181594902127E-4	0.0	0.0	14.226537739467469	0.0
32	5.383181594902127E-4	0.0	0.0	14.48493045602277	0.0
33	5.383181594902127E-4	0.0	0.0	14.655442733041296	0.0
34	5.383181594902127E-4	0.0	0.0	14.826762487299057	0.0
35	5.383181594902127E-4	0.0	0.0	15.008983184286492	0.0
36	5.383181594902127E-4	0.0	0.0	15.175188916029096	0.0
37	5.383181594902127E-4	0.0	0.0	15.34771988614571	0.0
38	5.383181594902127E-4	0.0	0.0	15.57812005840752	0.0
39	5.383181594902127E-4	0.0	0.0	15.935697895848895	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATCCG	20	7.031676E-4	45.0	17
TATCGCG	20	7.031676E-4	45.0	1
ACCCGAG	65	0.0	44.999996	41
CGATGAA	485	0.0	43.60825	19
CGTTTTT	13245	0.0	43.369198	1
CCGATGA	465	0.0	42.580647	18
ATCGAAT	85	0.0	42.35294	43
AACCCGA	70	0.0	41.785713	24
CGAATAT	65	0.0	41.53846	14
TTACACG	645	0.0	40.116276	34
CAATCGA	40	3.4560253E-7	39.375	41
TATGCGG	75	0.0	39.0	2
ACACGCG	660	0.0	38.863636	36
AACGACG	70	0.0	38.571426	11
TACACGC	685	0.0	37.773724	35
GTTTTTT	15870	0.0	37.698486	2
AAACACG	305	0.0	37.62295	40
GATGAAT	600	0.0	37.5	20
CGAACGA	30	1.13953356E-4	37.499996	22
ATTCGAC	30	1.13953356E-4	37.499996	11
>>END_MODULE