FastQCFastQC Report
Sat 18 Jun 2016
SRR3549549_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549549_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322455
Sequences flagged as poor quality0
Sequence length51
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197566.126746367710223No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG89572.7777519343784403No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC64812.009892853266347No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC38941.2076103642368703No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTTCGTT24850.7706501682405297No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC22660.7027337147819075No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT12930.40098618411871423No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC10550.3271774356111706No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT9430.2924439069017382No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTA7760.24065373462963824No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTGTTCGTTCGTATGCCG6930.2149137088896125No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT6400.19847730691104185Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC4460.13831387325363229No Hit
GAATGACTCTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT3670.11381433068180055No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGT3540.10978276038517001No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG3370.10451070692034548No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA3260.10109937820781195No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTG950.045.00000417
CCGATGA1250.045.00000418
GCGGGTA302.1606265E-645.0000045
CGGTGTA302.1606265E-645.00000419
TTTAGCG253.8845435E-545.01
AGACTCC207.0257304E-445.036
TGCTTCG207.0257304E-445.01
GGATAGG207.0257304E-445.08
TCGGACT207.0257304E-445.015
CTACGGC850.045.06
ACGGATC253.8845435E-545.030
GCTAGTG253.8845435E-545.012
TTAGTTG253.8845435E-545.01
TTCGGAC207.0257304E-445.014
CCGGTGT900.045.018
GAACATA207.0257304E-445.09
CACCGGT1000.045.016
TACGGCT9150.044.5081987
CGTTTTT86650.044.4287341
TGATACC12050.043.879674
Produced by FastQC (version 0.11.5)