##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549549_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	322455
Sequences flagged as poor quality	0
Sequence length	51
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.126054798343954	31.0	26.0	31.0	16.0	33.0
2	28.228230295700175	31.0	28.0	33.0	16.0	34.0
3	28.452962428866044	31.0	28.0	33.0	16.0	34.0
4	31.67237599044828	35.0	28.0	37.0	19.0	37.0
5	33.57148749437906	35.0	33.0	37.0	28.0	37.0
6	32.21643640197857	35.0	32.0	37.0	17.0	37.0
7	32.08075235304151	35.0	32.0	35.0	25.0	37.0
8	32.47322262021057	35.0	32.0	35.0	25.0	37.0
9	32.8723945976958	35.0	31.0	37.0	25.0	39.0
10	32.05377184413329	35.0	28.0	39.0	16.0	39.0
11	32.44725000387651	35.0	29.0	39.0	17.0	39.0
12	33.305099936425236	35.0	31.0	39.0	25.0	39.0
13	33.39496363833713	35.0	32.0	39.0	25.0	39.0
14	33.51215828565226	36.0	32.0	39.0	20.0	41.0
15	33.986788854258734	36.0	32.0	40.0	25.0	41.0
16	34.204831681940114	36.0	32.0	40.0	25.0	41.0
17	32.067314819122046	35.0	27.0	39.0	18.0	41.0
18	32.8290521157991	36.0	30.0	39.0	22.0	39.0
19	32.66322432587493	36.0	30.0	37.0	19.0	39.0
20	32.27691926005179	34.0	30.0	37.0	23.0	39.0
21	32.816027042533065	35.0	31.0	37.0	24.0	39.0
22	33.11133336434541	35.0	31.0	38.0	25.0	39.0
23	34.0204679722752	35.0	32.0	38.0	27.0	40.0
24	32.44265091253043	35.0	31.0	37.0	22.0	39.0
25	31.59876571924765	35.0	30.0	37.0	18.0	39.0
26	30.935625125986572	33.0	26.0	37.0	20.0	39.0
27	32.56860957342885	35.0	30.0	38.0	22.0	39.0
28	32.46986401203269	35.0	31.0	38.0	21.0	39.0
29	32.58495293916981	35.0	31.0	38.0	21.0	40.0
30	31.705106138841078	35.0	30.0	38.0	18.0	40.0
31	31.982896838318524	35.0	30.0	37.0	21.0	40.0
32	31.914611341117364	35.0	30.0	38.0	18.0	40.0
33	31.9562636646974	35.0	30.0	38.0	16.0	40.0
34	31.21542540819649	35.0	30.0	38.0	12.0	40.0
35	30.754325409747096	35.0	29.0	38.0	10.0	40.0
36	30.201609526910733	35.0	26.0	38.0	8.0	40.0
37	30.026574250670635	35.0	25.0	38.0	8.0	40.0
38	30.102969406583863	35.0	25.0	38.0	8.0	40.0
39	29.623702532136267	35.0	24.0	38.0	8.0	40.0
40	29.419556217146578	34.0	23.0	38.0	8.0	40.0
41	28.689727248763393	33.0	22.0	37.0	7.0	40.0
42	29.273107875517514	34.0	23.0	38.0	7.0	40.0
43	29.176297467863733	34.0	23.0	38.0	7.0	40.0
44	29.206236529128095	34.0	23.0	38.0	7.0	40.0
45	29.516648834721124	35.0	23.0	38.0	7.0	40.0
46	29.17245507125025	34.0	23.0	38.0	7.0	40.0
47	29.357256051231953	34.0	23.0	38.0	7.0	40.0
48	28.997723713386364	34.0	23.0	38.0	7.0	40.0
49	28.905459676544012	34.0	23.0	37.0	7.0	40.0
50	28.997425997425996	34.0	23.0	38.0	7.0	40.0
51	27.406692406692407	32.0	20.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	4.0
10	14.0
11	18.0
12	21.0
13	45.0
14	81.0
15	179.0
16	326.0
17	650.0
18	1126.0
19	1794.0
20	2628.0
21	3764.0
22	5198.0
23	6949.0
24	9596.0
25	12698.0
26	16060.0
27	17828.0
28	17836.0
29	18517.0
30	20272.0
31	23062.0
32	26206.0
33	29065.0
34	32414.0
35	34540.0
36	25439.0
37	13257.0
38	2811.0
39	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.528042672620987	2.5125986571769703	32.69355413933727	38.26580453086477
2	38.30581011303903	15.215766541067746	33.542974988758125	12.935448357135105
3	12.21968956908716	14.973562202477867	59.93425439208572	12.872493836349259
4	11.522538028562126	2.6509125304306025	70.67280705834924	15.153742382658045
5	21.238312322649673	3.359228419469383	59.26594408522119	16.136515172659752
6	13.398458699663518	13.591663953109737	61.32049433254253	11.68938301468422
7	51.602549192910644	1.1139538850382225	44.33393806887783	2.949558853173311
8	51.857158363182464	10.650168240529686	33.222310089779974	4.270363306507885
9	47.87861872199222	3.0084818036625265	33.4949062659906	15.617993208354655
10	25.509295870741656	22.614008156176833	38.667100835775535	13.20959513730598
11	23.779131971903055	15.988277434060565	47.20596672403901	13.026623869997364
12	20.010854227721698	13.829836721402986	48.222232559581954	17.937076491293357
13	17.151540525034502	15.572715572715573	54.833077483679894	12.442666418570033
14	14.785629002496472	19.354638631747065	46.969964801290104	18.889767564466357
15	11.169620567210929	16.554868121133183	56.151711091470126	16.123800220185764
16	12.43491339876882	15.630087919244545	47.77038656556729	24.164612116419345
17	12.741312741312742	17.552836829945264	52.45352064629173	17.252329782450264
18	12.983206959110575	15.657378548944815	50.89082197515933	20.46859251678529
19	13.740521933293017	18.971329332775117	47.57221937944829	19.71592935448357
20	16.667752089438835	17.1828627250314	53.38667410956568	12.76271107596409
21	17.41545331906778	21.990045122575243	46.05634894791522	14.538152610441768
22	14.5000077530198	15.75754756477648	49.63607325053108	20.10637143167264
23	14.781597432199842	22.08928377603076	46.88468158347676	16.24443720829263
24	15.970290428121753	16.245367570668776	47.169372470577294	20.614969530632184
25	13.494286024406508	23.0453861779163	44.89928827278225	18.561039524894944
26	14.82098277279	16.96949962010203	49.9520863376285	18.25743126947946
27	15.206773038098339	19.121117675334542	47.10331674187096	18.56879254469616
28	14.13561582236281	18.882324665457194	51.34731978105472	15.634739731125274
29	18.927292180304228	14.816951202493373	48.870695135755376	17.38506148144702
30	14.883937293575848	20.05985331286536	50.29507993363415	14.761129459924641
31	18.890697926842506	16.890108697337613	44.0486269401932	20.17056643562668
32	19.398365663425903	20.792978865268022	45.97416693802236	13.834488533283714
33	15.37889007768526	17.21976709928517	44.664526833201535	22.736815989828038
34	21.76458730675598	19.646152176272658	40.901831263277046	17.687429253694315
35	16.74993409933169	19.206400893147883	44.885953078724164	19.157711928796267
36	23.073607169992712	19.11305453474128	39.92060907723559	17.892729218030425
37	16.2090834379991	20.49464266331736	46.90670015971221	16.38957373897133
38	20.450915631638523	20.790187778139586	41.80831433843482	16.950582251787072
39	17.52089438836427	20.77933355041789	41.6265835542944	20.073188506923447
40	20.681335380130562	19.978291544556605	41.93825495030315	17.402118125009693
41	17.5556279170737	21.24482485928269	40.45029539005442	20.749251833589184
42	19.234001643640198	20.790187778139586	41.47803569490316	18.497774883317053
43	19.84059791288707	20.23383107720457	39.87502132080446	20.050549689103907
44	19.53574917430339	20.855003023677725	40.054891380192586	19.554356421826302
45	19.00141104960382	20.233210835620476	38.294335643733234	22.47104247104247
46	20.688158037555628	23.545300894698485	37.72154254081965	18.044998526926236
47	15.435642182630135	20.150408584143523	46.52742243103689	17.886526802189454
48	19.42751701787846	21.64674140577755	36.926082709215244	21.999658867128748
49	17.483369772526398	19.187173404040873	42.26388178195407	21.065575041478656
50	18.443503744708565	18.649423950628773	40.415561861345	22.491510443317672
51	18.47575630708161	18.474825944705465	38.64136081003551	24.40805693817742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	308.5
2	571.0
3	14838.0
4	29105.0
5	19785.0
6	10465.0
7	9885.0
8	9305.0
9	8891.0
10	8477.0
11	8024.5
12	7572.0
13	6952.5
14	6333.0
15	5713.5
16	5094.0
17	4669.0
18	4244.0
19	3893.5
20	3543.0
21	3312.5
22	3082.0
23	2953.0
24	2824.0
25	2785.0
26	2986.5
27	3227.0
28	3421.0
29	3615.0
30	3799.0
31	3983.0
32	4728.0
33	5473.0
34	5907.0
35	6341.0
36	7012.5
37	7684.0
38	7960.5
39	8237.0
40	8889.0
41	9541.0
42	10377.5
43	11214.0
44	12500.5
45	13787.0
46	27278.0
47	40769.0
48	33379.5
49	25990.0
50	23880.0
51	21770.0
52	17890.5
53	14011.0
54	12493.0
55	10975.0
56	10050.5
57	9126.0
58	8682.0
59	8238.0
60	7839.5
61	7441.0
62	6619.0
63	5797.0
64	4799.0
65	3801.0
66	3225.0
67	2649.0
68	2249.0
69	1849.0
70	1590.5
71	1332.0
72	1078.5
73	825.0
74	727.0
75	516.5
76	404.0
77	289.0
78	174.0
79	127.5
80	81.0
81	67.5
82	54.0
83	31.0
84	8.0
85	12.5
86	17.0
87	11.0
88	5.0
89	3.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	322455.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.755580414970495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.49134932903802	34.84209939362136
2	6.481937044797142	5.5427796112720085
3	2.6231182313912598	3.364588274406726
4	1.6811682695557333	2.875173005603478
5	1.314508584210305	2.8101288739186354
6	0.9891403324547225	2.537476141557512
7	0.8493124497383356	2.5418992719556095
8	0.7188205893038168	2.458687320793265
9	0.6265427174136099	2.4109377784012564
>10	3.1230979020097007	20.340208629910553
>50	0.053429943162154665	1.5775072211880747
>100	0.03879160256978353	3.2898895886431716
>500	0.0029276681184742287	0.955078761965087
>1k	0.003659585148092786	3.440098568244496
>5k	0.0014638340592371144	4.831096306427593
>10k+	7.319170296185572E-4	6.1823512520911725
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19756	6.126746367710223	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG	8957	2.7777519343784403	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGC	6481	2.009892853266347	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTC	3894	1.2076103642368703	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTTCGTT	2485	0.7706501682405297	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	2266	0.7027337147819075	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCT	1293	0.40098618411871423	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTC	1055	0.3271774356111706	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT	943	0.2924439069017382	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTA	776	0.24065373462963824	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTGTTCGTTCGTATGCCG	693	0.2149137088896125	No Hit
CTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGCT	640	0.19847730691104185	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCTTCTGC	446	0.13831387325363229	No Hit
GAATGACTCTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCGTCT	367	0.11381433068180055	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGT	354	0.10978276038517001	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCGTGTTCGTTCGTATGCCG	337	0.10451070692034548	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	326	0.10109937820781195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2369322851250562	0.0
2	0.0	0.0	0.0	1.333209285016514	0.0
3	0.0	0.0	0.0	1.5868880929121894	0.0
4	0.0	0.0	0.0	2.352576328479943	0.0
5	0.0	0.0	0.0	4.081499744150347	0.0
6	0.0	0.0	0.0	4.409297421345614	0.0
7	0.0	0.0	0.0	4.949217720302058	0.0
8	0.0	0.0	0.0	5.593338605386798	0.0
9	0.0	0.0	0.0	5.761113953885038	0.0
10	0.0	0.0	0.0	9.513265416879875	0.0
11	0.0	0.0	0.0	10.232125412848305	0.0
12	0.0	0.0	0.0	13.181684266021616	0.0
13	0.0	0.0	0.0	13.44590718084694	0.0
14	3.101207920485029E-4	0.0	0.0	13.612131925384938	0.0
15	3.101207920485029E-4	0.0	0.0	14.044750430292599	0.0
16	3.101207920485029E-4	0.0	0.0	14.362004000558217	0.0
17	3.101207920485029E-4	0.0	0.0	14.692282644089874	0.0
18	3.101207920485029E-4	0.0	0.0	14.983796188615466	0.0
19	3.101207920485029E-4	0.0	0.0	16.220868028096945	0.0
20	3.101207920485029E-4	0.0	0.0	16.585570079545985	0.0
21	3.101207920485029E-4	0.0	0.0	16.899412321099067	0.0
22	3.101207920485029E-4	0.0	0.0	17.421655734908747	0.0
23	3.101207920485029E-4	0.0	0.0	17.755965948737032	0.0
24	3.101207920485029E-4	0.0	0.0	18.021429346730553	0.0
25	3.101207920485029E-4	0.0	0.0	18.252779457598734	0.0
26	3.101207920485029E-4	0.0	0.0	18.46335147539967	0.0
27	3.101207920485029E-4	0.0	0.0	18.705555813989548	0.0
28	3.101207920485029E-4	0.0	0.0	18.913646865454094	0.0
29	3.101207920485029E-4	0.0	0.0	19.12421888325503	0.0
30	3.101207920485029E-4	0.0	0.0	19.348746336698145	0.0
31	3.101207920485029E-4	0.0	0.0	19.562419562419564	0.0
32	3.101207920485029E-4	0.0	0.0	19.79066846536726	0.0
33	3.101207920485029E-4	0.0	0.0	20.019227489107006	0.0
34	3.101207920485029E-4	0.0	0.0	20.253678807895675	0.0
35	3.101207920485029E-4	0.0	0.0	20.498053992029895	0.0
36	3.101207920485029E-4	0.0	0.0	20.717929633592284	0.0
37	3.101207920485029E-4	0.0	0.0	20.95083034842071	0.0
38	3.101207920485029E-4	0.0	0.0	21.179079251368407	0.0
39	3.101207920485029E-4	0.0	0.0	21.409809120652493	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTG	95	0.0	45.000004	17
CCGATGA	125	0.0	45.000004	18
GCGGGTA	30	2.1606265E-6	45.000004	5
CGGTGTA	30	2.1606265E-6	45.000004	19
TTTAGCG	25	3.8845435E-5	45.0	1
AGACTCC	20	7.0257304E-4	45.0	36
TGCTTCG	20	7.0257304E-4	45.0	1
GGATAGG	20	7.0257304E-4	45.0	8
TCGGACT	20	7.0257304E-4	45.0	15
CTACGGC	85	0.0	45.0	6
ACGGATC	25	3.8845435E-5	45.0	30
GCTAGTG	25	3.8845435E-5	45.0	12
TTAGTTG	25	3.8845435E-5	45.0	1
TTCGGAC	20	7.0257304E-4	45.0	14
CCGGTGT	90	0.0	45.0	18
GAACATA	20	7.0257304E-4	45.0	9
CACCGGT	100	0.0	45.0	16
TACGGCT	915	0.0	44.508198	7
CGTTTTT	8665	0.0	44.428734	1
TGATACC	1205	0.0	43.87967	4
>>END_MODULE