##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549548_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1001318
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.638576356362314	31.0	28.0	33.0	25.0	34.0
2	28.962103946997857	31.0	28.0	33.0	16.0	34.0
3	28.90862643036478	31.0	28.0	34.0	16.0	34.0
4	33.402081057166654	35.0	32.0	37.0	28.0	37.0
5	34.43240209404006	35.0	35.0	37.0	32.0	37.0
6	33.9351754387717	35.0	33.0	37.0	28.0	37.0
7	33.997062871135846	35.0	33.0	37.0	30.0	37.0
8	33.817710257880115	35.0	33.0	37.0	28.0	37.0
9	35.09656972110758	37.0	34.0	39.0	29.0	39.0
10	35.034480554629	37.0	34.0	39.0	28.0	39.0
11	35.44828815621012	37.0	34.0	39.0	30.0	39.0
12	35.55155804649472	37.0	34.0	39.0	30.0	39.0
13	35.80526466117657	37.0	35.0	39.0	30.0	39.0
14	36.561305199746734	38.0	35.0	41.0	30.0	41.0
15	36.925539139414255	39.0	36.0	41.0	31.0	41.0
16	36.84000587226036	39.0	35.0	41.0	31.0	41.0
17	36.0586726694217	38.0	34.0	40.0	28.0	41.0
18	35.73192731979251	38.0	34.0	39.0	29.0	40.0
19	34.98363257226975	37.0	34.0	38.0	27.0	40.0
20	34.05688802158755	35.0	33.0	37.0	27.0	40.0
21	34.291070369253326	35.0	33.0	38.0	29.0	40.0
22	34.64009835037421	35.0	33.0	38.0	29.0	40.0
23	34.91688554485189	35.0	33.0	39.0	30.0	40.0
24	34.298825148454334	35.0	33.0	38.0	27.0	40.0
25	33.321896740096555	35.0	33.0	38.0	24.0	40.0
26	33.20814466533109	35.0	32.0	38.0	23.0	40.0
27	34.08388943372635	35.0	33.0	38.0	25.0	40.0
28	33.705960544003005	35.0	33.0	38.0	24.0	40.0
29	33.59102403032803	35.0	33.0	39.0	23.0	40.0
30	32.71167601101748	35.0	32.0	38.0	20.0	40.0
31	32.53493695309582	35.0	32.0	38.0	18.0	40.0
32	31.576512156977103	35.0	30.0	38.0	15.0	40.0
33	30.472010889647446	35.0	24.0	39.0	8.0	40.0
34	29.48276771215538	35.0	21.0	39.0	7.0	40.0
35	28.719072262757685	35.0	18.0	39.0	7.0	40.0
36	28.030779432707693	35.0	15.0	39.0	7.0	40.0
37	27.74322343151726	35.0	14.0	39.0	7.0	40.0
38	27.67030254125063	35.0	14.0	39.0	7.0	40.0
39	27.30394340259538	34.0	12.0	39.0	7.0	40.0
40	27.22886136072656	34.0	12.0	39.0	7.0	40.0
41	26.895831294354043	33.0	10.0	38.0	7.0	40.0
42	26.952834164571094	34.0	10.0	39.0	7.0	40.0
43	26.87480400831704	33.0	10.0	39.0	7.0	40.0
44	26.866995300194343	34.0	10.0	39.0	7.0	40.0
45	26.996087157126908	34.0	10.0	39.0	7.0	40.0
46	26.737440054008815	33.0	10.0	39.0	7.0	40.0
47	26.625216964041392	33.0	10.0	39.0	7.0	40.0
48	26.422729842068154	33.0	10.0	38.0	7.0	40.0
49	26.512854058351092	33.0	10.0	39.0	7.0	40.0
50	26.47717907797523	33.0	10.0	39.0	7.0	40.0
51	25.537843122764198	31.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	29.0
10	44.0
11	64.0
12	100.0
13	138.0
14	194.0
15	335.0
16	663.0
17	1438.0
18	2864.0
19	5094.0
20	8279.0
21	12191.0
22	18271.0
23	26712.0
24	39976.0
25	57598.0
26	70361.0
27	65753.0
28	53035.0
29	43719.0
30	39958.0
31	41868.0
32	46316.0
33	54362.0
34	63690.0
35	75947.0
36	88350.0
37	96027.0
38	76674.0
39	11258.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.402591384555155	2.040810212140399	28.427832117269443	48.12876628603501
2	46.4357976187385	13.675076249503155	28.454896446483534	11.434229685274808
3	9.632404490881019	13.73170161726844	64.16972430336816	12.46616958848238
4	8.547134876233125	2.2482368238661445	76.02949312805723	13.175135171843511
5	17.488849696100537	2.6067642846728014	66.40497823868142	13.499407780545242
6	10.918309667857763	12.018359801781251	68.8176982736753	8.245632256685688
7	44.87755138727157	0.8327024981074944	52.348105197349895	1.941640917271037
8	44.551581016220624	8.745872939465785	44.21532420270084	2.4872218416127545
9	42.03140261135823	2.2648149738644467	43.50316283138823	12.200619583389093
10	23.780157752082758	12.766873261041948	51.85914964077346	11.593819346101837
11	18.886707319752567	15.185984871938786	54.0097151953725	11.917592612936149
12	13.000365518246952	13.019140772461895	58.05728050429534	15.923213204995815
13	13.247539742619226	13.885498912433414	63.15536123389373	9.711600111053631
14	13.14207874022039	18.34012771167601	55.721858590377884	12.795934957725718
15	10.370631507672886	14.5922673915779	63.189516217625176	11.847584883124043
16	10.851198120876685	15.379130306256354	56.18564731683641	17.58402425603055
17	11.621782490677287	15.973247260111172	59.62681186196593	12.778158387245611
18	11.517020566892835	15.39151398456834	57.94093384918677	15.150531599352055
19	11.83340357408935	17.31038491268508	55.707377676222734	15.148833837002831
20	13.462156877235804	15.85220679144887	60.21953065859198	10.466105672723351
21	13.399539407061493	19.444771790779754	56.213210987917925	10.942477814240831
22	12.199321294533805	13.468748189885732	58.37426272173276	15.957667793847708
23	12.1139338352052	19.246932542908446	57.084063204696214	11.555070417190143
24	13.514987246808708	15.817152992356077	55.363830471438646	15.304029289396576
25	10.901232175992043	22.1630890486339	54.16471091101928	12.770967864354779
26	12.000383494554177	15.291545742711108	57.818095749801756	14.889975012932954
27	14.018523585913767	17.20432470004534	54.737655769695536	14.039495944345354
28	11.205231504876572	16.79486436876197	58.73159176205761	13.268312364303847
29	16.41466547090934	14.599757519589183	54.69031816066424	14.295258848837234
30	11.67171667741916	18.066887841824474	58.216970033495855	12.044425447260512
31	14.316031470521853	16.164295458585585	53.94689798845123	15.572775082441343
32	14.566101877725156	21.08640811410561	50.865559192983646	13.481930815185587
33	13.860332082315507	17.33884739912795	49.669835157262725	19.130985361293813
34	20.61403070752748	19.64560708985557	44.4693893448435	15.270972857773454
35	14.566501351219094	20.936305948759536	44.319586784617876	20.1776059154035
36	20.29275415003026	20.286562310874267	42.23872935471049	17.18195418438498
37	16.107170748952882	24.85424210890047	43.32100291815387	15.717584223992779
38	17.346637132259684	22.5686545133514	43.18128706365011	16.903421290738805
39	21.55509039086484	24.47274492219255	38.302517282222034	15.669647404720576
40	17.265244407870426	22.633169482621902	41.856333352641215	18.24525275686645
41	21.859589061616788	24.2124879408939	37.95028152894485	15.97764146854446
42	16.778386087137154	23.890911778276234	40.35371380520474	18.976988329381875
43	21.57646222279036	22.41026327300618	37.661062719335916	18.352211784867546
44	19.201292696226375	23.253851423823402	38.02628136116598	19.51857451878424
45	16.65544811937866	22.854278061514925	38.62978594212827	21.860487876978144
46	20.92552016442329	24.55963040712341	36.51057905680313	18.004270371650165
47	16.384904695611187	21.710485580005553	43.71108878498139	18.193520939401868
48	19.48032493173997	23.679490431611136	36.07555242190792	20.76463221474097
49	16.652152463053696	22.254768215491982	40.51110636181513	20.581972959639195
50	19.11221010707887	21.206649635780042	38.794668626749946	20.886471630391146
51	18.175045290307377	21.763315949578455	37.090414833249774	22.971223926864393
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	1423.0
2	2781.0
3	35480.0
4	68179.0
5	53464.5
6	38750.0
7	37235.5
8	35721.0
9	35392.5
10	35064.0
11	34443.5
12	33823.0
13	32911.5
14	32000.0
15	30734.5
16	29469.0
17	27937.5
18	26406.0
19	24681.0
20	22956.0
21	21674.5
22	20393.0
23	19331.0
24	18269.0
25	17703.5
26	16437.5
27	15737.0
28	16165.5
29	16594.0
30	17248.0
31	17902.0
32	19231.5
33	20561.0
34	21776.5
35	22992.0
36	24588.0
37	26184.0
38	27223.0
39	28262.0
40	30499.5
41	32737.0
42	34526.5
43	36316.0
44	39472.5
45	42629.0
46	85687.5
47	128746.0
48	91277.0
49	53808.0
50	49606.0
51	45404.0
52	39572.0
53	33740.0
54	30785.0
55	27830.0
56	24397.0
57	20964.0
58	18632.0
59	16300.0
60	14301.5
61	12303.0
62	10314.0
63	8325.0
64	6772.5
65	5220.0
66	4221.0
67	3222.0
68	2501.0
69	1780.0
70	1453.0
71	1126.0
72	911.0
73	696.0
74	575.0
75	351.0
76	248.0
77	186.0
78	124.0
79	83.0
80	42.0
81	31.0
82	20.0
83	24.5
84	29.0
85	17.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1001318.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.299431147142556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.31515582806747	41.20673494725482
2	5.030475547637439	4.859382147009975
3	2.083546681005654	3.0190235838326993
4	1.3374691296775905	2.5839599256116585
5	1.0004909425551565	2.4161571696641273
6	0.8493654711859647	2.4614321456584083
7	0.7102892537020524	2.4014596832616286
8	0.6039243191281516	2.3335360855852083
9	0.5031566711972737	2.187196289704635
>10	2.472769306325604	17.987486468371262
>50	0.050980060770634446	1.6923929019796735
>100	0.0369223506108481	3.3019948903092766
>500	0.0029370051502261595	1.0479081587203647
>1k	0.0014685025751130798	2.333323252660274
>5k	2.097860821590114E-4	0.5279057732482207
>10k+	8.391443286360456E-4	9.640106577127778
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33045	3.3001504017704666	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCG	21541	2.1512646332134246	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	20498	2.047101919669875	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGC	20056	2.002960098590058	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT	5210	0.5203142258503293	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTATGCGT	4653	0.4646875418198814	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTC	4465	0.4459122876049367	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTA	3846	0.3840937644184964	No Hit
GCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	3709	0.3704117972512229	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT	3448	0.34434615177196454	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTATGCGTCGT	1537	0.15349769004452132	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTT	1370	0.13681967167273534	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.053629316560772904	0.0
2	0.0	0.0	0.0	0.4873576626006923	0.0
3	0.0	0.0	0.0	0.5579646026537024	0.0
4	0.0	0.0	0.0	1.181742463433195	0.0
5	0.0	0.0	0.0	3.5692956683091683	0.0
6	0.0	0.0	0.0	3.7908037206961227	0.0
7	0.0	0.0	0.0	4.237614823662413	0.0
8	0.0	0.0	0.0	4.8043678431826855	0.0
9	0.0	0.0	0.0	4.885660699198456	0.0
10	0.0	0.0	0.0	7.427510541106821	0.0
11	0.0	0.0	0.0	7.584303887476306	0.0
12	0.0	0.0	0.0	10.040666401682582	0.0
13	0.0	0.0	0.0	10.227320391723708	0.0
14	0.0	0.0	0.0	10.371130849540306	0.0
15	0.0	0.0	0.0	10.871671137440853	0.0
16	0.0	0.0	0.0	11.115250100367716	0.0
17	0.0	0.0	0.0	11.238487673246661	0.0
18	0.0	0.0	0.0	11.340553150947052	0.0
19	0.0	0.0	0.0	11.997087838229215	0.0
20	0.0	0.0	0.0	12.141896979780649	0.0
21	0.0	0.0	0.0	12.248456534287808	0.0
22	0.0	0.0	0.0	12.446096045412148	0.0
23	0.0	0.0	0.0	12.566737040580515	0.0
24	0.0	0.0	0.0	12.671299227618	0.0
25	0.0	0.0	0.0	12.756187345079185	0.0
26	0.0	0.0	0.0	12.85405835109326	0.0
27	0.0	0.0	0.0	12.99477288933186	0.0
28	0.0	0.0	0.0	13.086052582696007	0.0
29	0.0	0.0	0.0	13.188417665516848	0.0
30	0.0	0.0	0.0	13.305862872733737	0.0
31	0.0	0.0	0.0	13.41412018959012	0.0
32	0.0	0.0	0.0	13.534761184758489	0.0
33	0.0	0.0	0.0	13.647812183542092	0.0
34	0.0	0.0	0.0	13.806602897381252	0.0
35	0.0	0.0	0.0	13.925246525079945	0.0
36	0.0	0.0	0.0	14.0465865988627	0.0
37	0.0	0.0	0.0	14.186002848246012	0.0
38	0.0	0.0	0.0	14.324020940400553	0.0
39	0.0	0.0	0.0	14.478617182553394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGAC	35	1.2117198E-7	45.000004	9
CTTCGCG	45	3.8380676E-10	45.0	1
TACGAGT	20	7.0328516E-4	45.0	29
TCGAATG	20	7.0328516E-4	45.0	44
CGTTTTT	36140	0.0	44.340065	1
CGGCTGT	2510	0.0	42.310757	9
TACGGCT	2475	0.0	42.18182	7
ACGGCTG	2495	0.0	42.02405	8
ACATACG	75	0.0	42.0	17
GCGACTG	440	0.0	41.93182	11
TGATACC	2545	0.0	41.817287	4
ACGGCCT	140	0.0	41.785717	8
CGATGAA	625	0.0	41.760002	19
GATACCT	2555	0.0	41.741684	5
GACCCGT	65	0.0	41.53846	38
CCGATGA	635	0.0	41.102364	18
CGCTTTT	945	0.0	40.95238	1
GGCGACT	465	0.0	40.645157	10
ATACCTG	2640	0.0	40.482952	6
ATGATAC	8375	0.0	40.325375	3
>>END_MODULE