##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549547_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595590
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.830863513490826	31.0	28.0	31.0	16.0	33.0
2	29.012491814839066	31.0	30.0	33.0	16.0	34.0
3	29.130205342601453	31.0	30.0	33.0	16.0	34.0
4	32.46044930237244	35.0	32.0	37.0	19.0	37.0
5	33.92677848855757	35.0	33.0	37.0	30.0	37.0
6	33.01402810658339	35.0	33.0	37.0	25.0	37.0
7	32.7336691348075	35.0	32.0	37.0	27.0	37.0
8	33.20840007387633	35.0	33.0	37.0	28.0	37.0
9	33.52093554290703	35.0	32.0	39.0	26.0	39.0
10	32.64502594066388	37.0	30.0	39.0	17.0	39.0
11	33.403494014338726	37.0	31.0	39.0	19.0	39.0
12	34.02492318541278	37.0	32.0	39.0	26.0	39.0
13	34.305634748736544	37.0	32.0	39.0	27.0	39.0
14	34.382204200876444	37.0	32.0	40.0	24.0	41.0
15	34.98964388253665	37.0	32.0	40.0	27.0	41.0
16	35.14723887237865	38.0	32.0	40.0	27.0	41.0
17	33.2406773115734	36.0	30.0	40.0	19.0	41.0
18	33.72643429204654	36.0	32.0	39.0	25.0	39.0
19	33.425740862002385	37.0	32.0	37.0	24.0	39.0
20	32.71977366980641	35.0	32.0	36.0	25.0	39.0
21	32.95808525999429	35.0	32.0	37.0	25.0	39.0
22	32.860257895532165	34.0	30.0	37.0	25.0	39.0
23	33.86163132356151	35.0	33.0	38.0	27.0	40.0
24	32.10558269950805	35.0	31.0	37.0	21.0	39.0
25	31.292352121425814	34.0	29.0	37.0	18.0	39.0
26	30.093159723971187	33.0	25.0	36.0	18.0	39.0
27	32.217926761698486	34.0	30.0	37.0	22.0	39.0
28	32.153476384761326	35.0	31.0	37.0	21.0	39.0
29	32.09338303195151	35.0	30.0	38.0	21.0	40.0
30	31.160741449654964	34.0	27.0	37.0	18.0	39.0
31	31.508645208952466	35.0	30.0	37.0	18.0	39.0
32	31.066676740710893	35.0	30.0	37.0	15.0	40.0
33	30.351693278933496	35.0	25.0	38.0	10.0	40.0
34	28.893171477022783	34.0	21.0	38.0	7.0	40.0
35	28.06967712688259	34.0	18.0	38.0	7.0	40.0
36	27.383795899864	33.0	15.0	38.0	7.0	40.0
37	27.183110864856697	33.0	15.0	38.0	7.0	40.0
38	27.31985090414547	33.0	15.0	38.0	7.0	40.0
39	26.944238486206956	33.0	15.0	38.0	7.0	40.0
40	26.834438120183346	33.0	15.0	37.0	7.0	40.0
41	26.42250877281351	32.0	13.0	37.0	7.0	40.0
42	26.718131600597726	33.0	13.0	38.0	7.0	40.0
43	26.71232223509461	33.0	12.0	38.0	7.0	40.0
44	26.62555449218422	33.0	11.0	38.0	7.0	40.0
45	26.856297117144344	33.0	11.0	38.0	7.0	40.0
46	26.580139021810307	33.0	10.0	38.0	7.0	40.0
47	26.576683624641113	33.0	10.0	38.0	7.0	40.0
48	26.296855219194413	33.0	10.0	37.0	7.0	40.0
49	26.040680669588138	32.0	10.0	37.0	7.0	40.0
50	26.19991772863883	33.0	10.0	37.0	7.0	40.0
51	24.777403918803202	30.0	10.0	35.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	10.0
10	11.0
11	37.0
12	55.0
13	73.0
14	158.0
15	311.0
16	719.0
17	1270.0
18	2453.0
19	4245.0
20	6466.0
21	9657.0
22	13422.0
23	18688.0
24	25734.0
25	35067.0
26	41402.0
27	38745.0
28	33046.0
29	30589.0
30	31815.0
31	35629.0
32	41122.0
33	46816.0
34	52474.0
35	55069.0
36	42267.0
37	23106.0
38	5033.0
39	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	19.18971104283148	2.0295841098742424	30.960560116858915	47.820144730435366
2	48.13982773384375	10.970130458872715	29.459527527325847	11.43051427995769
3	9.960207525311036	10.862338185664635	66.90626101848586	12.271193270538458
4	9.081750868886314	2.2711932705384577	74.97741735086217	13.669638509713058
5	16.209976661797544	2.6499773334004937	67.80855957957655	13.331486425225405
6	11.290149263755268	9.706677412313839	69.6165147164996	9.386658607431286
7	45.62215618126564	0.8425258986886952	51.38551688241911	2.1498010376265553
8	46.65877533202371	7.169361473496869	43.306972917611105	2.8648902768683158
9	43.28061250188888	2.2161218287748286	43.306133413925686	11.197132255410601
10	22.978559075874344	13.640087979986232	51.366544099128596	12.01480884501083
11	17.98233684245874	15.677227623029266	54.41276717204788	11.92766836246411
12	13.40888866502124	13.427357746100505	57.865477929448105	15.298275659430145
13	13.342903675347134	14.288352725868467	62.453365570274855	9.915378028509545
14	13.106163636058362	16.664316056347488	56.44957101361675	13.7799492939774
15	10.45064557833409	15.063550428986384	62.24903037324334	12.236773619436189
16	10.825232122769018	14.370120384828489	56.82096744404708	17.983680048355414
17	11.595560704511493	15.246226430934032	59.62709246293591	13.531120401618562
18	11.641397605735488	14.593596265887607	58.24510149599556	15.519904632381337
19	12.075588911835323	16.968384291207038	55.95073792373948	15.005288873218154
20	13.431723165264694	15.77461005053812	59.88801020836482	10.905656575832367
21	13.64075958293457	18.610957202102117	55.88945415470374	11.858829060259575
22	12.243993351130811	14.03482261287127	57.97075169159993	15.750432344397991
23	12.03865074967679	19.103074262496012	56.35487499790124	12.503399989925956
24	12.966134421330109	15.595627864806328	55.90288621367048	15.535351500193087
25	11.155660773350796	19.966084051108986	54.37582900988935	14.502426165650867
26	11.871589516277977	15.952752732584496	57.679108111284606	14.496549639852919
27	12.766668345674038	17.17372689266106	55.67504491344717	14.384559848217734
28	11.100085629375913	16.628049497137294	59.035913967662324	13.235950905824476
29	15.221041320371395	14.605853019694756	55.558186000436535	14.614919659497305
30	11.730888698601387	17.43615574472372	58.237713863563854	12.595241693111033
31	13.690626101848588	16.249097533538173	54.77694387078359	15.283332493829649
32	13.769371547541093	19.908997800500345	52.788159640020815	13.533471011937742
33	13.379002333820244	16.826172366896692	51.39643043032959	18.398394868953474
34	19.119864336204436	19.13228899074867	45.95023422152823	15.797612451518662
35	14.1548716398865	20.34570761765644	45.61627965546769	19.883141086989372
36	18.70179150086469	19.39119192733256	43.99637334407898	17.910643227723767
37	15.486996087912827	24.028106583387903	44.36860927819473	16.116288050504544
38	16.622172971339346	21.97434476737353	44.782148793633205	16.621333467653923
39	20.329589146896353	23.483268691549554	40.451820883493674	15.735321278060411
40	17.37638308232173	21.395087224432917	42.96596652059302	18.262563172652328
41	21.070031397437834	22.46310381302574	39.99899259557749	16.467872193958932
42	16.90340670595544	22.641078594335028	41.706039389512924	18.749475310196612
43	20.67747947413489	21.593210094192315	39.8263906378549	17.902919793817894
44	18.55235984485972	22.136704780133982	40.34839402944979	18.96254134555651
45	16.551990463238134	21.766483654863244	40.641548716398866	21.039977165499756
46	19.683507110596217	23.468157625211976	38.61549052200339	18.232844742188416
47	16.259675279974477	20.64809684514515	45.015530818180295	18.076697056700077
48	18.971104283147803	22.122769018955992	38.62909048170721	20.27703621618899
49	16.421195789049513	20.9170738259541	41.3462281099414	21.31550227505499
50	18.64000402961769	19.885659598045635	40.47750969626757	20.99682667606911
51	17.88293960610487	20.342685404388924	39.00904984972884	22.765325139777364
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	78.0
1	398.0
2	718.0
3	25672.5
4	50627.0
5	36821.5
6	23016.0
7	22051.0
8	21086.0
9	20769.0
10	20452.0
11	19948.5
12	19445.0
13	18845.5
14	18246.0
15	17406.0
16	16566.0
17	15601.0
18	14636.0
19	13694.5
20	12753.0
21	11906.5
22	11060.0
23	10449.5
24	9839.0
25	9434.0
26	8732.0
27	8435.0
28	8589.0
29	8743.0
30	9265.5
31	9788.0
32	10660.5
33	11533.0
34	12369.5
35	13206.0
36	14231.5
37	15257.0
38	16131.0
39	17005.0
40	18523.5
41	20042.0
42	21606.0
43	23170.0
44	25150.5
45	27131.0
46	44525.0
47	61919.0
48	50367.0
49	38815.0
50	35727.0
51	32639.0
52	27707.0
53	22775.0
54	19959.0
55	17143.0
56	14676.0
57	12209.0
58	10727.0
59	9245.0
60	8087.0
61	6929.0
62	5752.0
63	4575.0
64	3788.5
65	3002.0
66	2400.5
67	1799.0
68	1405.0
69	1011.0
70	825.0
71	639.0
72	536.5
73	434.0
74	366.5
75	234.0
76	169.0
77	116.5
78	64.0
79	44.5
80	25.0
81	22.5
82	20.0
83	16.0
84	12.0
85	7.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	595590.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.53364866276951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.48244945638629	43.021552197928244
2	6.3677560945022975	6.563074106885813
3	2.793322879479188	4.318503596182683
4	1.821338102168827	3.754407914131349
5	1.2614386680389034	3.250326856417439
6	0.9227213211891063	2.853071782788353
7	0.7130482264743552	2.5722183747918224
8	0.5435269409685101	2.2407941131696845
9	0.4422686996240505	2.0512547802869183
>10	1.56703548313551	11.781707132770856
>50	0.044209059030007616	1.6024862537667317
>100	0.03490188817325517	3.5946738260859057
>500	0.002991590414850441	1.017848552518582
>1k	0.0016619946749169114	1.5646295319260735
>5k	6.647978699667646E-4	2.530232408154153
>10k+	6.647978699667646E-4	7.2832185721954
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	32034	5.37853221175641	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCG	10484	1.7602713275911281	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGC	8506	1.4281636696385098	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	6265	1.0518981178327371	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTATGCGT	2280	0.3828136805520576	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT	2081	0.3494014338722947	No Hit
GCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1858	0.3119595695025101	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTC	1547	0.25974244026931276	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTA	1368	0.22968820833123457	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT	927	0.1556439832770866	No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCGTTATGCGTCGTATGCCG	857	0.14389093168119008	No Hit
GAATCTTTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	685	0.11501200490270153	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCC	651	0.1093033798418375	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT	596	0.10006883930220453	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.679007370842358E-4	0.0	0.0	0.08999479507715039	0.0
2	1.679007370842358E-4	0.0	0.0	0.5685118957672224	0.0
3	1.679007370842358E-4	0.0	0.0	0.712738628922581	0.0
4	1.679007370842358E-4	0.0	0.0	1.3064356352524387	0.0
5	1.679007370842358E-4	0.0	0.0	2.737117815947212	0.0
6	1.679007370842358E-4	0.0	0.0	3.033966319112141	0.0
7	1.679007370842358E-4	0.0	0.0	3.392938094998237	0.0
8	1.679007370842358E-4	0.0	0.0	3.923504424184422	0.0
9	1.679007370842358E-4	0.0	0.0	4.082170620729025	0.0
10	1.679007370842358E-4	0.0	0.0	6.377373696670529	0.0
11	1.679007370842358E-4	0.0	0.0	6.949075706442351	0.0
12	1.679007370842358E-4	0.0	0.0	9.081918769623398	0.0
13	1.679007370842358E-4	0.0	0.0	9.333937775986836	0.0
14	1.679007370842358E-4	0.0	0.0	9.474470692926342	0.0
15	1.679007370842358E-4	0.0	0.0	9.90530398428449	0.0
16	1.679007370842358E-4	0.0	0.0	10.178142682046374	0.0
17	1.679007370842358E-4	0.0	0.0	10.449134471700331	0.0
18	1.679007370842358E-4	0.0	0.0	10.719622559143035	0.0
19	1.679007370842358E-4	0.0	0.0	11.42262294531473	0.0
20	1.679007370842358E-4	0.0	0.0	11.683372790006548	0.0
21	3.358014741684716E-4	0.0	0.0	11.986601521180678	0.0
22	3.358014741684716E-4	0.0	0.0	12.38704477912658	0.0
23	3.358014741684716E-4	0.0	0.0	12.714115414966672	0.0
24	3.358014741684716E-4	0.0	0.0	12.955220873419634	0.0
25	3.358014741684716E-4	0.0	0.0	13.164593092563676	0.0
26	3.358014741684716E-4	0.0	0.0	13.353985123994695	0.0
27	3.358014741684716E-4	0.0	0.0	13.550932688594504	0.0
28	3.358014741684716E-4	0.0	0.0	13.75812219815645	0.0
29	3.358014741684716E-4	0.0	0.0	13.965647509192566	0.0
30	3.358014741684716E-4	1.679007370842358E-4	0.0	14.205409761748854	0.0
31	3.358014741684716E-4	1.679007370842358E-4	0.0	14.432915260497994	0.0
32	3.358014741684716E-4	1.679007370842358E-4	0.0	14.64917140986249	0.0
33	3.358014741684716E-4	1.679007370842358E-4	0.0	14.851491798048993	0.0
34	3.358014741684716E-4	1.679007370842358E-4	0.0	15.066908443728067	0.0
35	3.358014741684716E-4	1.679007370842358E-4	0.0	15.287865813730923	0.0
36	3.358014741684716E-4	1.679007370842358E-4	0.0	15.502778757198744	0.0
37	3.358014741684716E-4	1.679007370842358E-4	0.0	15.72104971540825	0.0
38	3.358014741684716E-4	1.679007370842358E-4	0.0	15.942678688359441	0.0
39	3.358014741684716E-4	1.679007370842358E-4	0.0	16.167497775315233	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCCG	95	0.0	45.000004	4
CGTTCGG	40	6.8048394E-9	45.000004	2
TACGCCA	20	7.030545E-4	45.000004	13
CGTTTCG	20	7.030545E-4	45.000004	2
CCGCTTG	35	1.2106102E-7	45.0	1
CTACGCC	25	3.888534E-5	45.0	12
CGTTTTT	22700	0.0	44.454845	1
CCGATGA	150	0.0	43.5	18
CGATGAA	135	0.0	43.333332	19
ACGGCTG	1110	0.0	42.77027	8
TACGGCT	1120	0.0	42.38839	7
TGATACC	1435	0.0	42.1777	4
CCGGGTA	70	0.0	41.785713	5
CGGCTGT	1060	0.0	41.603775	9
GACCCGT	65	0.0	41.538464	38
GATACCT	1455	0.0	41.443302	5
GAATGAT	4055	0.0	41.115906	1
ATGATAC	3805	0.0	40.742443	3
TGCTACG	105	0.0	40.714287	4
CGGCGAC	1220	0.0	40.573772	9
>>END_MODULE