FastQCFastQC Report
Sat 18 Jun 2016
SRR3549544_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549544_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences661878
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260443.9348641290388864No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGC191952.9000812838619807No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCG185142.7971922318010267No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTC174892.642329855350986No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT47970.7247559217861902No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTTTCGT43820.6620555449795884No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTA37490.566418584693856No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTC36600.5529719978606329No Hit
GCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC33430.5050779750951082No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCT31630.47788263093802785No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGT16140.2438515859418201No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTT11230.1696687304911177No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTAT9940.15017873384521016No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATG8910.13461695357754752No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7440.11240742251593194No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA6970.10530641598602763No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCCGA253.889005E-545.00000425
CGACGAA207.0311147E-445.00000419
CGGTTAT253.889005E-545.00000431
CGACCGT207.0311147E-445.00000412
GTCGTTG302.1641008E-645.0000041
GCGATTA302.1641008E-645.0000049
AGGGCCG302.1641008E-645.0000046
GCCGTTG302.1641008E-645.0000041
AGGTCGA207.0311147E-445.00000411
CGTTTTT227100.044.4649931
CGGCTGT24400.043.0635269
ACGGCTG24350.042.8747448
GATACCT24550.042.708765
TACGGCT24350.042.689947
TGATACC24850.042.193164
CGCTTTT6050.042.024791
CTTTGCG700.041.7857131
CACCCGT1400.041.78571316
CGATGAA5550.041.75675619
GAATCTG21000.041.4642871
Produced by FastQC (version 0.11.5)