##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549544_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	661878
Sequences flagged as poor quality	0
Sequence length	51
%GC	33
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.391644683763474	31.0	28.0	31.0	25.0	34.0
2	28.57373262141966	31.0	27.0	33.0	16.0	34.0
3	28.495706157328087	31.0	27.0	33.0	16.0	34.0
4	33.20161721646588	35.0	32.0	37.0	28.0	37.0
5	34.29084514064525	35.0	33.0	37.0	30.0	37.0
6	33.62110540008884	35.0	33.0	37.0	28.0	37.0
7	33.90721552914585	35.0	33.0	37.0	30.0	37.0
8	33.576213441147765	35.0	33.0	37.0	28.0	37.0
9	34.95948195891086	37.0	34.0	39.0	28.0	39.0
10	34.91457942400261	37.0	34.0	39.0	28.0	39.0
11	35.29230009155766	37.0	34.0	39.0	30.0	39.0
12	35.39164619461592	37.0	34.0	39.0	30.0	39.0
13	35.52555455839293	37.0	34.0	39.0	30.0	39.0
14	36.35103750237959	38.0	35.0	40.0	30.0	41.0
15	36.56622066302249	38.0	34.0	41.0	30.0	41.0
16	36.6422422259087	38.0	34.0	41.0	31.0	41.0
17	35.75431121747513	38.0	33.0	40.0	27.0	41.0
18	35.56014703616074	38.0	34.0	39.0	28.0	40.0
19	34.830885752359194	37.0	34.0	38.0	27.0	40.0
20	33.68534835725013	35.0	32.0	37.0	27.0	39.0
21	34.10426997120315	35.0	33.0	38.0	27.0	40.0
22	34.537574900510364	35.0	33.0	38.0	29.0	40.0
23	34.77314550415635	35.0	33.0	39.0	30.0	40.0
24	34.24493788885565	35.0	33.0	38.0	27.0	40.0
25	33.23922686658266	35.0	33.0	38.0	24.0	40.0
26	33.11337890064332	35.0	31.0	38.0	23.0	40.0
27	33.96185399726234	35.0	33.0	38.0	25.0	40.0
28	33.60463106493946	35.0	33.0	38.0	24.0	40.0
29	33.44787256866069	35.0	33.0	38.0	23.0	40.0
30	32.677868126754475	35.0	32.0	38.0	21.0	40.0
31	32.62149822172666	35.0	32.0	38.0	20.0	40.0
32	31.736664460822084	35.0	31.0	38.0	15.0	40.0
33	30.619021934555914	35.0	25.0	38.0	10.0	40.0
34	29.76040297456631	35.0	22.0	39.0	7.0	40.0
35	29.13109666736166	35.0	19.0	39.0	7.0	40.0
36	28.39761708351086	35.0	16.0	39.0	7.0	40.0
37	28.29513596161226	35.0	15.0	39.0	7.0	40.0
38	28.065054284928642	35.0	15.0	39.0	7.0	40.0
39	27.72381617156032	34.0	15.0	38.0	7.0	40.0
40	27.55229513596161	34.0	15.0	38.0	7.0	40.0
41	27.21518920405271	33.0	15.0	38.0	7.0	40.0
42	27.275346816180626	34.0	13.0	38.0	7.0	40.0
43	27.137909101073006	34.0	12.0	38.0	7.0	40.0
44	27.227564596496634	34.0	12.0	39.0	7.0	40.0
45	27.32497076500503	34.0	12.0	39.0	7.0	40.0
46	27.1035069907143	34.0	10.0	38.0	7.0	40.0
47	27.014662823058025	33.0	10.0	38.0	7.0	40.0
48	26.86948501083281	33.0	10.0	38.0	7.0	40.0
49	26.878105934930606	33.0	10.0	38.0	7.0	40.0
50	26.79741281625919	33.0	10.0	38.0	7.0	40.0
51	25.788816065800646	31.0	10.0	37.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	13.0
9	30.0
10	26.0
11	40.0
12	63.0
13	98.0
14	121.0
15	211.0
16	409.0
17	873.0
18	1853.0
19	3291.0
20	5436.0
21	8038.0
22	11655.0
23	16468.0
24	24118.0
25	35038.0
26	43160.0
27	42050.0
28	35617.0
29	30658.0
30	29267.0
31	31175.0
32	34690.0
33	40915.0
34	46284.0
35	53719.0
36	58512.0
37	59452.0
38	42547.0
39	6050.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.14503277038971	2.2087152012908726	27.20818640293226	43.43806562538715
2	44.73634113839711	18.009361241799848	26.591003175811856	10.66329444399119
3	9.802410716174279	18.1616551690795	60.50193540199251	11.533998712753709
4	8.453521645983097	2.2472419388467357	76.56169263822034	12.737543776949831
5	20.73660100501905	2.9043116707308596	62.64492852157044	13.714158802679648
6	10.620990575302397	15.677058309839579	65.55815422177501	8.143796893083016
7	42.61631297610738	1.0168037009841693	54.10966975787078	2.2572135650376657
8	41.41986891844116	12.116130162960545	43.295743324298435	3.168257594299856
9	39.18818875986209	2.1475256769374416	42.26896799712333	16.395317566077132
10	25.309649210277424	13.060110775701867	49.36498871393217	12.265251300088536
11	20.98120801718746	14.514306261879078	52.485654455957146	12.018831264976324
12	14.637289651567206	12.59824318076745	55.68352475833915	17.08094240932619
13	14.715249638150837	14.104563076579069	61.556812584796596	9.623374700473502
14	13.58105270155527	19.95337509329514	52.78117719579741	13.684395009352176
15	9.63908756598648	14.599216169747296	62.94181102861857	12.819885235647657
16	10.727052417515011	16.01851096425625	53.16387612218566	20.09056049604308
17	11.317040300478336	17.1233973632603	58.01930265094172	13.54025968531965
18	11.560891886420157	15.910031758118565	56.054439035592665	16.474637319868616
19	11.832089901764373	18.260464919516888	53.311788577351116	16.595656601367622
20	14.330888774064102	16.29182417303491	58.93110210643049	10.446184946470497
21	13.776405923750298	21.8821293350134	53.57588558616543	10.765579155070874
22	12.196658598714567	13.599334016238643	57.33609517161773	16.86791221342906
23	12.940904517146665	20.832389050550102	54.74301910624012	11.483687326063112
24	14.118160748657607	16.503645686969502	52.751413402469936	16.62678016190295
25	10.532605706791887	24.820133015449976	51.372307283215335	13.274953994542802
26	11.7793611511487	16.071995141098512	56.10233305835819	16.046310649394602
27	15.226673193549264	18.935664880839067	51.06288470080589	14.77477722480578
28	10.153834996781884	19.000631536325425	56.39558951951871	14.449943947373987
29	14.902595342344057	17.450648004617165	52.6282789275365	15.018477725502283
30	14.17768833531255	20.430804468497215	53.36164670830576	12.029860487884473
31	14.302484747944485	16.25420394695095	50.0211519343444	19.42215937076017
32	15.440156645182345	21.02290754489498	50.607362686174795	12.92957312374788
33	14.21636615811373	17.282641211824533	48.04858297148417	20.452409658577565
34	16.23063464868148	22.398236533016657	45.808744209657974	15.562384608643889
35	17.00872366206461	20.698829693689774	41.69680817310743	20.595638471138187
36	17.50171481753435	23.837323494662158	39.01927545559756	19.641686232205934
37	17.972949697678427	21.995594354246553	44.670467971438846	15.360987976636178
38	16.66017000111803	22.30244848748561	40.02127280254065	21.01610870885571
39	17.53782419116514	22.44205125415862	44.579816824248574	15.440307730427664
40	15.732959850606909	22.736516397281676	42.79942829343172	18.731095458679697
41	18.92811061857321	24.67871722583316	40.109959841541794	16.283212314051834
42	15.351469606181201	22.820066537942036	41.53771540978851	20.29074844608825
43	20.36613998350149	23.186448257836037	37.46113332064217	18.9862784380203
44	18.598895869027224	22.901199314677328	38.384868510511	20.11503630578445
45	15.148411036474998	22.232647104149102	38.0740861608937	24.5448556984822
46	21.079262341398262	24.591692124530503	36.90332659493139	17.42571893913984
47	14.424561626160711	21.728324555280583	45.727762518168	18.119351300390708
48	17.669419439836346	24.408878977696798	36.699361513753296	21.222340068713567
49	15.24631427544049	21.53977016912482	42.20249048918381	21.01142506625088
50	18.322258784851588	21.36889275667117	39.12548838305549	21.183360075421753
51	16.506667391875844	22.192911684630705	36.9060461293471	24.394374794146355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	1090.5
2	2129.0
3	25658.0
4	49187.0
5	37944.0
6	26701.0
7	25671.5
8	24642.0
9	24182.0
10	23722.0
11	22903.0
12	22084.0
13	21134.5
14	20185.0
15	19032.0
16	17879.0
17	16791.5
18	15704.0
19	14749.5
20	13795.0
21	12854.5
22	11914.0
23	11197.0
24	10480.0
25	10153.5
26	9654.5
27	9482.0
28	9848.5
29	10215.0
30	10424.0
31	10633.0
32	11206.5
33	11780.0
34	12539.0
35	13298.0
36	14480.0
37	15662.0
38	16051.5
39	16441.0
40	17394.0
41	18347.0
42	19993.0
43	21639.0
44	23613.5
45	25588.0
46	64484.0
47	103380.0
48	71142.0
49	38904.0
50	34325.0
51	29746.0
52	25127.0
53	20508.0
54	18182.0
55	15856.0
56	14542.0
57	13228.0
58	12135.5
59	11043.0
60	9891.5
61	8740.0
62	7582.5
63	6425.0
64	5512.0
65	4599.0
66	3716.5
67	2834.0
68	2294.5
69	1755.0
70	1551.5
71	1348.0
72	1110.0
73	872.0
74	719.5
75	480.5
76	394.0
77	278.5
78	163.0
79	112.5
80	62.0
81	46.0
82	30.0
83	27.5
84	25.0
85	15.0
86	5.0
87	4.0
88	3.0
89	3.0
90	3.0
91	2.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	661878.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.38259970598155
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.91635229575256	42.42772833619238
2	5.933189431616991	5.859926373626041
3	2.264015268131905	3.3540887914316504
4	1.3113359354236094	2.590287103163719
5	0.860913686618397	2.125707798383857
6	0.6148413521297167	1.8217478624943748
7	0.4888440725009091	1.6898273805667954
8	0.40947713586394996	1.6176836391297011
9	0.31983172755288863	1.4214709957515166
>10	1.7823855146567824	14.253749049591427
>50	0.055621481343778326	1.9386720000211077
>100	0.03635593894821208	3.424056179935643
>500	0.003107345209248875	1.044832774454679
>1k	0.0024858761673991	3.963735555432342
>5k	0.0	0.0
>10k+	0.00124293808369955	12.466486159824795
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26044	3.9348641290388864	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGC	19195	2.9000812838619807	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCG	18514	2.7971922318010267	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTC	17489	2.642329855350986	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCT	4797	0.7247559217861902	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGCTTTCGT	4382	0.6620555449795884	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTA	3749	0.566418584693856	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTC	3660	0.5529719978606329	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	3343	0.5050779750951082	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCT	3163	0.47788263093802785	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGT	1614	0.2438515859418201	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTT	1123	0.1696687304911177	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTAT	994	0.15017873384521016	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATG	891	0.13461695357754752	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	744	0.11240742251593194	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA	697	0.10530641598602763	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09367285209660994	0.0
2	0.0	0.0	0.0	0.6859270137396922	0.0
3	0.0	0.0	0.0	0.7850389346677182	0.0
4	0.0	0.0	0.0	1.6524193280332629	0.0
5	0.0	0.0	0.0	4.747400578354319	0.0
6	0.0	0.0	0.0	5.008022626526339	0.0
7	0.0	0.0	0.0	5.620068955305963	0.0
8	0.0	0.0	0.0	6.324730539464977	0.0
9	0.0	0.0	0.0	6.418101221070953	0.0
10	0.0	0.0	0.0	9.704205306718157	0.0
11	0.0	0.0	0.0	9.925998446843678	0.0
12	0.0	0.0	0.0	13.40277211208108	0.0
13	0.0	0.0	0.0	13.652516022590266	0.0
14	0.0	0.0	0.0	13.86373319554359	0.0
15	0.0	0.0	0.0	14.604050897597443	0.0
16	0.0	0.0	0.0	14.971188043718026	0.0
17	0.0	0.0	0.0	15.154907702023635	0.0
18	0.0	0.0	0.0	15.293755042470062	0.0
19	0.0	0.0	0.0	16.238491081437967	0.0
20	0.0	0.0	0.0	16.442758333106706	0.0
21	0.0	0.0	0.0	16.61363574556036	0.0
22	0.0	0.0	0.0	16.901755308380093	0.0
23	0.0	0.0	0.0	17.096202019103217	0.0
24	0.0	0.0	0.0	17.254086100459602	0.0
25	0.0	0.0	0.0	17.386738945848027	0.0
26	0.0	0.0	0.0	17.540241555090212	0.0
27	0.0	0.0	0.0	17.72562315109431	0.0
28	0.0	0.0	0.0	17.860844445653125	0.0
29	0.0	0.0	0.0	18.02235457289712	0.0
30	0.0	0.0	0.0	18.190059195199115	0.0
31	0.0	0.0	0.0	18.363353971577844	0.0
32	0.0	0.0	0.0	18.540123708598866	0.0
33	0.0	0.0	0.0	18.71205871776974	0.0
34	0.0	0.0	0.0	18.9297725562717	0.0
35	0.0	0.0	0.0	19.12346384076824	0.0
36	0.0	0.0	0.0	19.30506830563941	0.0
37	0.0	0.0	0.0	19.52686144576493	0.0
38	0.0	0.0	0.0	19.744877454757525	0.0
39	0.0	0.0	0.0	19.974073771903583	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCCGA	25	3.889005E-5	45.000004	25
CGACGAA	20	7.0311147E-4	45.000004	19
CGGTTAT	25	3.889005E-5	45.000004	31
CGACCGT	20	7.0311147E-4	45.000004	12
GTCGTTG	30	2.1641008E-6	45.000004	1
GCGATTA	30	2.1641008E-6	45.000004	9
AGGGCCG	30	2.1641008E-6	45.000004	6
GCCGTTG	30	2.1641008E-6	45.000004	1
AGGTCGA	20	7.0311147E-4	45.000004	11
CGTTTTT	22710	0.0	44.464993	1
CGGCTGT	2440	0.0	43.063526	9
ACGGCTG	2435	0.0	42.874744	8
GATACCT	2455	0.0	42.70876	5
TACGGCT	2435	0.0	42.68994	7
TGATACC	2485	0.0	42.19316	4
CGCTTTT	605	0.0	42.02479	1
CTTTGCG	70	0.0	41.785713	1
CACCCGT	140	0.0	41.785713	16
CGATGAA	555	0.0	41.756756	19
GAATCTG	2100	0.0	41.464287	1
>>END_MODULE