FastQCFastQC Report
Sat 18 Jun 2016
SRR3549542_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549542_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344088
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG140344.078607798005161No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC115643.3607681755829906No Hit
GAATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC104703.042826253749041No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87542.5441166213294273No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACTAGGGT26840.7800330148101648No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTC24410.7094115458836111No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTA23280.6765711097161191No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT22440.6521587500871869No Hit
GAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT21640.6289088837739183No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC21430.6228057938666853No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGT7990.2322080398037711No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT6190.17989584059891656No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTAT5850.17001464741577738No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATG5440.15809909093022714No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC302.1610522E-645.00000417
ATCACAT302.1610522E-645.00000432
CACCGGT950.045.00000416
CGGACAG207.026389E-445.012
CGGGTTC207.026389E-445.06
CGGGTCT207.026389E-445.06
CCGCTGG253.8850892E-545.02
TCTACGG207.026389E-445.02
TCGCTTG253.8850892E-545.01
GCGATAT253.8850892E-545.09
ATGCGGC207.026389E-445.06
CCGGTGA406.7902874E-945.018
ATCGCAG207.026389E-445.024
GCGAGGG406.7902874E-945.03
CGTTTTT73750.044.1457631
GATACCT16350.043.623855
TGATACC16450.043.358664
GCGACTG2950.042.71186411
ACGGCTG14200.042.147898
GCGACCA11900.041.9747911