##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549542_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	344088
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.198417265350724	31.0	28.0	31.0	25.0	34.0
2	28.0788112343354	31.0	25.0	33.0	16.0	34.0
3	28.15391120876055	31.0	25.0	33.0	16.0	34.0
4	32.85965799446653	35.0	32.0	37.0	28.0	37.0
5	34.13754039664272	35.0	33.0	37.0	30.0	37.0
6	33.222175722489595	35.0	32.0	37.0	28.0	37.0
7	33.44011706307689	35.0	32.0	37.0	29.0	37.0
8	33.16801515891284	35.0	32.0	37.0	28.0	37.0
9	34.55419253214294	37.0	34.0	39.0	28.0	39.0
10	34.47562832763712	37.0	32.0	39.0	27.0	39.0
11	34.72325393503987	37.0	34.0	39.0	27.0	39.0
12	34.80687498546883	37.0	34.0	39.0	27.0	39.0
13	34.86952465648322	37.0	34.0	39.0	27.0	39.0
14	35.73773278928646	38.0	34.0	40.0	27.0	41.0
15	35.92469368301132	38.0	34.0	40.0	27.0	41.0
16	35.963936551114834	38.0	34.0	40.0	30.0	41.0
17	35.159735300272025	37.0	33.0	40.0	27.0	41.0
18	35.008166515542534	37.0	33.0	39.0	27.0	40.0
19	34.40526260724001	37.0	32.0	38.0	27.0	40.0
20	33.673449815163565	35.0	32.0	38.0	25.0	40.0
21	34.32779986515077	35.0	33.0	38.0	27.0	40.0
22	34.904594173583504	35.0	33.0	39.0	29.0	40.0
23	35.09490014182418	35.0	33.0	39.0	29.0	40.0
24	34.7450681221083	35.0	33.0	39.0	27.0	40.0
25	33.782203971077166	35.0	33.0	38.0	24.0	40.0
26	33.8265065913371	35.0	32.0	38.0	25.0	40.0
27	34.49491409174397	35.0	33.0	39.0	27.0	40.0
28	34.07892167120039	35.0	33.0	39.0	25.0	40.0
29	34.192886703401456	35.0	33.0	39.0	24.0	40.0
30	33.59399339703797	35.0	33.0	39.0	23.0	40.0
31	33.719039896770596	35.0	33.0	39.0	23.0	40.0
32	33.18021262002743	35.0	33.0	39.0	20.0	40.0
33	32.7112105042896	36.0	32.0	39.0	16.0	40.0
34	32.312100392922744	36.0	31.0	39.0	12.0	40.0
35	31.966988096068448	36.0	31.0	40.0	10.0	41.0
36	31.49118539443398	36.0	30.0	40.0	8.0	41.0
37	31.41420218083746	36.0	30.0	40.0	8.0	41.0
38	31.261871962986213	35.0	29.0	40.0	8.0	40.0
39	30.910932668387158	35.0	27.0	39.0	8.0	40.0
40	30.60345028016089	35.0	25.0	39.0	8.0	40.0
41	30.27980051614703	35.0	24.0	39.0	8.0	40.0
42	30.391286531352446	35.0	24.0	39.0	7.0	40.0
43	30.35731266420218	35.0	24.0	39.0	7.0	40.0
44	30.454322731394296	35.0	24.0	39.0	7.0	40.0
45	30.68855350956732	36.0	25.0	39.0	7.0	40.0
46	30.42492036920788	35.0	24.0	39.0	7.0	40.0
47	30.286380228313686	35.0	24.0	39.0	7.0	40.0
48	30.12621480551487	35.0	24.0	39.0	7.0	40.0
49	30.40111250610309	35.0	25.0	39.0	7.0	40.0
50	30.33061600520797	35.0	24.0	39.0	7.0	40.0
51	28.859861430796773	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	9.0
9	18.0
10	15.0
11	21.0
12	30.0
13	41.0
14	44.0
15	81.0
16	153.0
17	277.0
18	712.0
19	1233.0
20	1941.0
21	2690.0
22	4029.0
23	5414.0
24	7692.0
25	10919.0
26	14363.0
27	16479.0
28	15520.0
29	14866.0
30	15788.0
31	18047.0
32	21285.0
33	25402.0
34	28918.0
35	33341.0
36	35966.0
37	36092.0
38	28398.0
39	4304.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.66286531352445	2.4127548766594593	30.47737788007719	34.4470019297389
2	33.00986956824998	21.948745669712398	32.17113064099881	12.870254121038805
3	12.277091906721536	21.79239031875567	52.81614005719467	13.114377717328127
4	10.953593266838716	2.77690590779103	71.21782799748901	15.05167282788124
5	25.705342819278787	3.50956731998791	52.86496477645254	17.920125084280766
6	13.21783962242217	19.452872520983007	57.03947827299993	10.289809583594895
7	51.85737369510125	1.2406709911418008	43.89138824951757	3.0105670642393805
8	50.13281486131454	15.168503406105415	30.914184743437723	3.7844969891423124
9	47.07603868777754	2.8315430936272117	29.55813629071633	20.534281927878915
10	30.822638394829227	18.940503592104346	36.138139080700284	14.09871893236614
11	26.797505289344585	15.610541489386437	43.223245216340004	14.36870800492897
12	20.57090046732231	13.498291134825974	45.1547278603148	20.77608053753691
13	18.7821138778452	14.62561902769059	54.04954546511358	12.54272162935063
14	16.01654227988189	22.32190602404036	43.17471112041106	18.48684057566669
15	11.845806886610402	15.318174420497083	56.43817860547302	16.397840087419496
16	13.792692567017738	17.4222873218479	43.352572597707564	25.432447513426798
17	14.360861175048242	18.462428216037758	49.932284764362606	17.244425844551394
18	14.008916323731139	17.449896538094904	47.824975006393714	20.716212131780242
19	14.261177373230105	20.23435865243775	44.214561391272	21.289902583060147
20	17.45396526469973	18.821929273906676	50.99247866824766	12.731626793145939
21	17.42170607519007	25.531550068587105	43.289216712003906	13.75752714421892
22	14.734602776034036	15.57857292320569	47.55237032387063	22.134453976889635
23	15.330089976982633	24.46786868475506	45.255574155448606	14.9464671828137
24	18.149136267466464	17.22146660156704	43.02852758596638	21.60086954500012
25	13.515437911232011	28.196856618074445	41.312396828718235	16.97530864197531
26	14.067622236172141	16.208063053637442	48.22719769361326	21.497117016577157
27	17.685882681174583	20.403210806537864	41.427193043639996	20.483713468647554
28	11.810350840482666	20.238136755713654	49.503324730882795	18.44818767292088
29	18.43685336309316	16.55070795842924	46.25735277022157	18.755085908256028
30	14.222524470484293	26.96984492339169	43.80013252423798	15.007498081886029
31	18.551940201343843	16.519320638906326	40.50649833763456	24.422240822115274
32	21.085303759503383	23.031317569923974	41.93898072586082	13.944397944711817
33	17.625723652089	21.872602357536444	36.34390039757272	24.157773592801842
34	23.24143823673014	19.266873590476855	41.41673060379903	16.074957568993977
35	19.710364790402455	25.39844458394364	35.63332635837344	19.257864267280464
36	20.438957475994513	21.556113552347075	36.320069284601615	21.684859687056797
37	17.388865639022573	25.891341749784942	41.49636139592197	15.22343121527051
38	18.878310199716353	26.782974122898796	31.481190858152562	22.85752481923229
39	24.019436888237895	22.101904164051057	37.51627490641929	16.362384041291765
40	21.243112227104692	25.77828927483667	35.420299458278116	17.55829903978052
41	25.349038618027947	24.01391504498849	31.77268605705517	18.86436027992839
42	20.10997186766176	22.224547208853547	39.21671200390598	18.44876891957871
43	22.974936644114297	21.577329055357932	33.011613308223474	22.436120992304296
44	21.165806421613077	22.98946781056009	34.556276301411266	21.288449466415567
45	17.624561158773336	20.927785915230988	35.105554393062235	26.342098532933434
46	24.185382809048846	25.20808630350376	32.7628397386715	17.843691148775896
47	15.261212248029574	22.569226476947758	43.568505731092046	18.601055543930624
48	21.66742228732185	23.237660133454234	31.963625584152894	23.13129199507103
49	18.0343400525447	19.821382902048313	40.705284694613006	21.438992350793985
50	20.650821882774174	19.335460696101	36.96874055474181	23.044976866383017
51	19.235776894282857	20.12334054079189	34.454558136290714	26.186324428634535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	52.0
1	512.5
2	973.0
3	10661.0
4	20349.0
5	15209.5
6	10070.0
7	9610.5
8	9151.0
9	8831.0
10	8511.0
11	7986.5
12	7462.0
13	6918.5
14	6375.0
15	5796.5
16	5218.0
17	4829.5
18	4441.0
19	4010.5
20	3580.0
21	3264.5
22	2949.0
23	2808.0
24	2667.0
25	2673.5
26	2929.5
27	3179.0
28	3709.0
29	4239.0
30	4310.0
31	4381.0
32	4877.0
33	5373.0
34	6189.0
35	7005.0
36	7077.0
37	7149.0
38	7893.0
39	8637.0
40	9847.5
41	11058.0
42	11372.0
43	11686.0
44	13727.5
45	15769.0
46	40485.0
47	65201.0
48	44085.5
49	22970.0
50	20728.5
51	18487.0
52	16089.5
53	13692.0
54	12592.0
55	11492.0
56	11320.5
57	11149.0
58	10608.0
59	10067.0
60	9575.5
61	9084.0
62	7816.5
63	6549.0
64	5533.5
65	4518.0
66	3627.5
67	2737.0
68	2313.0
69	1889.0
70	1601.5
71	1314.0
72	1069.5
73	825.0
74	687.0
75	424.5
76	300.0
77	225.5
78	151.0
79	119.5
80	88.0
81	63.5
82	39.0
83	32.0
84	25.0
85	15.0
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	344088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.79289129802619
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.07376220648617	33.888189514072664
2	5.360230615927638	4.373186096957762
3	2.1353546478421532	2.6132187009647985
4	1.4146272119705654	2.308269363405805
5	1.0955046949484202	2.234440196875412
6	0.9965095372098852	2.4390303137309535
7	0.8131973044729686	2.3220868471649605
8	0.7392986941104167	2.412650501249516
9	0.6198305298490434	2.2756211484596998
>10	3.63372995146804	22.919832116309042
>50	0.07649177461498226	2.1259007025310876
>100	0.031454561523917936	2.190056827984701
>500	0.002859505593083449	0.7427529615016023
>1k	0.004289258389625174	4.083829003874534
>5k	7.148763982708623E-4	2.5528305555496766
>10k+	0.002144629194812587	10.518105149367802
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCG	14034	4.078607798005161	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGC	11564	3.3607681755829906	No Hit
GAATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTC	10470	3.042826253749041	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8754	2.5441166213294273	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTACTAGGGT	2684	0.7800330148101648	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTC	2441	0.7094115458836111	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTA	2328	0.6765711097161191	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCT	2244	0.6521587500871869	No Hit
GAACTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCT	2164	0.6289088837739183	No Hit
GCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTTCTGC	2143	0.6228057938666853	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTACTAGGGTCGT	799	0.2322080398037711	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATGCCGTCTT	619	0.17989584059891656	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTAT	585	0.17001464741577738	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTACTAGGGTCGTATG	544	0.15809909093022714	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09183697193741136	0.0
2	0.0	0.0	0.0	0.831182720699356	0.0
3	0.0	0.0	0.0	0.9331915091488224	0.0
4	0.0	0.0	0.0	1.7024714607891005	0.0
5	0.0	0.0	0.0	5.3059101160168325	0.0
6	0.0	0.0	0.0	5.58258352514473	0.0
7	0.0	0.0	0.0	6.381507056334426	0.0
8	0.0	0.0	0.0	7.2955174257748014	0.0
9	0.0	0.0	0.0	7.424554183813443	0.0
10	0.0	0.0	0.0	12.269535700169724	0.0
11	0.0	0.0	0.0	12.48110948362047	0.0
12	0.0	0.0	0.0	16.535014298667782	0.0
13	0.0	0.0	0.0	16.83435632745112	0.0
14	0.0	0.0	0.0	17.088651740252494	0.0
15	0.0	0.0	0.0	17.972146660156703	0.0
16	0.0	0.0	0.0	18.35228197437865	0.0
17	0.0	0.0	0.0	18.52113412847876	0.0
18	0.0	0.0	0.0	18.660342703029457	0.0
19	0.0	0.0	0.0	19.75018018646393	0.0
20	0.0	0.0	0.0	19.96262583990142	0.0
21	0.0	0.0	0.0	20.098928181162957	0.0
22	0.0	0.0	0.0	20.40117644323545	0.0
23	0.0	0.0	0.0	20.53166631791867	0.0
24	0.0	0.0	0.0	20.65808746599707	0.0
25	0.0	0.0	0.0	20.758933761130873	0.0
26	0.0	0.0	0.0	20.90221106228639	0.0
27	0.0	0.0	0.0	21.094313082699774	0.0
28	0.0	0.0	0.0	21.196031247820326	0.0
29	0.0	0.0	0.0	21.317511799307155	0.0
30	0.0	0.0	0.0	21.447420427332542	0.0
31	0.0	0.0	0.0	21.576457185371183	0.0
32	0.0	0.0	0.0	21.7214782265002	0.0
33	0.0	0.0	0.0	21.848480621236426	0.0
34	0.0	0.0	0.0	22.062670014647416	0.0
35	0.0	0.0	0.0	22.212922275696915	0.0
36	0.0	0.0	0.0	22.347771500313872	0.0
37	0.0	0.0	0.0	22.490176931482644	0.0
38	0.0	0.0	0.0	22.6378135825719	0.0
39	0.0	0.0	0.0	22.80346888005394	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTC	30	2.1610522E-6	45.000004	17
ATCACAT	30	2.1610522E-6	45.000004	32
CACCGGT	95	0.0	45.000004	16
CGGACAG	20	7.026389E-4	45.0	12
CGGGTTC	20	7.026389E-4	45.0	6
CGGGTCT	20	7.026389E-4	45.0	6
CCGCTGG	25	3.8850892E-5	45.0	2
TCTACGG	20	7.026389E-4	45.0	2
TCGCTTG	25	3.8850892E-5	45.0	1
GCGATAT	25	3.8850892E-5	45.0	9
ATGCGGC	20	7.026389E-4	45.0	6
CCGGTGA	40	6.7902874E-9	45.0	18
ATCGCAG	20	7.026389E-4	45.0	24
GCGAGGG	40	6.7902874E-9	45.0	3
CGTTTTT	7375	0.0	44.145763	1
GATACCT	1635	0.0	43.62385	5
TGATACC	1645	0.0	43.35866	4
GCGACTG	295	0.0	42.711864	11
ACGGCTG	1420	0.0	42.14789	8
GCGACCA	1190	0.0	41.97479	11
>>END_MODULE