FastQCFastQC Report
Sat 18 Jun 2016
SRR3549539_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549539_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409231
Sequences flagged as poor quality0
Sequence length51
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330228.069281163939193No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCG76781.8762019495101787No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC58081.4192473199733158No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC47531.1614467134698985No Hit
GAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCT14940.365074982100574No Hit
GCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC13820.33770657648125385No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGCTTCGT13750.3359960511300464No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTC9980.2438720429292991No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTA7630.18644726328161845No Hit
GAATGATACCTGTCTCTTCTACACATCTGACGCTTGCTTCGTCGTATGCCG6320.1544360031375922No Hit
GAATGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT5830.14246232567913966No Hit
GAATGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTT5520.134887141980935No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA5470.1336653381586439No Hit
GAATCTTTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC5030.1229134645224824No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC5010.122424742993566No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC4440.10849617941944771No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT4380.10703001483269842No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA4200.10263152107245052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA302.1620617E-645.00000416
CACCGGT1650.045.00000416
TGATCCG850.045.04
CGTTGAT253.886388E-545.025
GGGTACA502.1827873E-1145.07
CGATTGC207.0279563E-445.010
AGTTGAC207.0279563E-445.032
CGCAACG207.0279563E-445.016
TATACGG502.1827873E-1145.027
TATCGGG253.886388E-545.03
TCGCGCG207.0279563E-445.01
TAATCGT253.886388E-545.021
CGTTTTT149150.044.5776021
CCGATGA1400.043.39285718
CGATGAA1350.043.33333219
TGATACC10000.042.34
ACGGCTG8250.042.08
ACCCTTG700.041.78571339
GAATGAT32400.041.527781
TACGGCT8350.041.4970057
Produced by FastQC (version 0.11.5)