##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549539_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 409231 Sequences flagged as poor quality 0 Sequence length 51 %GC 33 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.646827830736186 31.0 28.0 31.0 16.0 33.0 2 28.87870420373823 31.0 30.0 33.0 16.0 34.0 3 28.988781397303722 31.0 30.0 33.0 16.0 34.0 4 32.16996024250362 35.0 30.0 37.0 19.0 37.0 5 33.7187138804245 35.0 33.0 37.0 28.0 37.0 6 32.85841248585762 35.0 33.0 37.0 18.0 37.0 7 32.446459334703384 35.0 32.0 37.0 26.0 37.0 8 33.016355065965186 35.0 32.0 37.0 27.0 37.0 9 33.326182522829406 35.0 32.0 38.0 25.0 39.0 10 32.2585727865191 35.0 28.0 39.0 15.0 39.0 11 32.99284756042431 37.0 31.0 39.0 17.0 39.0 12 33.77119768541484 37.0 31.0 39.0 25.0 39.0 13 33.89634460732447 37.0 32.0 39.0 25.0 39.0 14 34.17987884593298 37.0 32.0 40.0 22.0 41.0 15 34.628266187067936 37.0 32.0 40.0 25.0 41.0 16 34.79536252141211 37.0 32.0 40.0 25.0 41.0 17 32.852237489339764 36.0 29.0 40.0 18.0 41.0 18 33.37040693398105 36.0 31.0 39.0 23.0 39.0 19 33.03739452778504 36.0 31.0 37.0 23.0 39.0 20 32.35022029122916 34.0 31.0 35.0 24.0 39.0 21 32.7132817406306 35.0 31.0 36.0 24.0 39.0 22 32.64243911140652 34.0 30.0 37.0 24.0 39.0 23 33.68067912743658 35.0 32.0 38.0 27.0 39.0 24 31.95297521448766 34.0 30.0 36.0 21.0 39.0 25 31.221796491468144 34.0 29.0 36.0 18.0 39.0 26 30.04419508785991 33.0 25.0 36.0 18.0 39.0 27 32.01919942526348 34.0 30.0 37.0 22.0 39.0 28 32.07936104547309 35.0 31.0 37.0 21.0 39.0 29 32.14339089658408 35.0 30.0 38.0 21.0 40.0 30 31.257920343278002 34.0 29.0 37.0 18.0 40.0 31 31.58148087510477 34.0 30.0 37.0 20.0 39.0 32 31.322328953573898 35.0 30.0 37.0 17.0 39.0 33 30.819432056711246 35.0 28.0 38.0 14.0 40.0 34 29.652641173322646 35.0 24.0 38.0 8.0 40.0 35 28.930691956376716 34.0 22.0 37.0 7.0 40.0 36 28.089152581304937 34.0 18.0 37.0 7.0 40.0 37 28.125581884070368 34.0 18.0 38.0 7.0 40.0 38 28.05602703607498 34.0 18.0 38.0 7.0 40.0 39 27.64606298154343 33.0 18.0 37.0 7.0 40.0 40 27.43574900239718 33.0 18.0 37.0 7.0 40.0 41 26.93089721941886 33.0 15.0 37.0 7.0 40.0 42 27.297907538773945 33.0 17.0 37.0 7.0 40.0 43 27.259577109261027 33.0 15.0 37.0 7.0 40.0 44 27.21638634414304 33.0 15.0 37.0 7.0 40.0 45 27.415359540210787 33.0 15.0 38.0 7.0 40.0 46 27.127888649686852 33.0 15.0 37.0 7.0 40.0 47 27.11845876778641 33.0 15.0 37.0 7.0 40.0 48 26.922623163934308 33.0 15.0 37.0 7.0 40.0 49 26.67132988458841 33.0 15.0 37.0 7.0 39.0 50 26.83039408060484 33.0 15.0 37.0 7.0 40.0 51 25.44159411188302 30.0 12.0 35.0 7.0 38.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 8.0 10 20.0 11 26.0 12 39.0 13 57.0 14 118.0 15 249.0 16 500.0 17 899.0 18 1673.0 19 2823.0 20 4187.0 21 6000.0 22 8337.0 23 11420.0 24 15814.0 25 21292.0 26 25966.0 27 26502.0 28 24521.0 29 23152.0 30 24290.0 31 27196.0 32 30568.0 33 33425.0 34 36985.0 35 39338.0 36 27050.0 37 13973.0 38 2745.0 39 56.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.687582319032526 2.2151303298137237 30.2271822027168 46.87010514843695 2 47.682115968731594 11.461497296148142 29.292502278664127 11.563884456456133 3 10.50995647934785 11.391121395984175 65.89774479450482 12.20117733016316 4 9.793979439485279 2.5706752421004273 73.75198848572077 13.883356832693517 5 17.019971605279167 2.8011074429845246 65.42906084827396 14.749860103462348 6 12.05602703607498 9.961855284668072 68.00853307789488 9.973584601362067 7 45.20967375394337 1.0761648066739813 51.173542571310584 2.540618868072067 8 45.88606434996371 7.621612243451742 43.316855272450034 3.1754681341345106 9 42.358961075773834 2.3158069647705086 43.19027639646068 12.134955562994982 10 24.619835740694132 14.599578233320546 48.90440851255159 11.876177513433733 11 19.657112975312234 13.959353030440019 54.39788285833673 11.985651135911013 12 16.22824272843455 12.205820184687866 56.67019360703368 14.895743479843905 13 14.592491771151256 13.30886467545225 61.11487155176416 10.98377200163233 14 13.123639215992924 15.86952112620989 56.00235563776938 15.004484020027808 15 10.338903944227098 14.039747721946775 61.980886101004074 13.640462232822049 16 11.801891841038435 13.849879407962739 55.12143508189751 19.226793669101312 17 12.480726044703358 14.756213483338263 58.00953495702916 14.753525514929223 18 12.480237323174443 13.860142560069985 57.213163225659834 16.446456891095735 19 12.914466401616693 16.393186244443847 54.24980023507505 16.442547118864407 20 14.272623530475453 15.613431045057682 58.41004224997618 11.70390317449069 21 14.871307403398081 18.37299715808431 54.50149182246702 12.254203616050592 22 13.42689092468557 13.408563867351203 56.621321454142034 16.54322375382119 23 13.364334568984265 18.488824160437503 54.94989382524784 13.196947445330387 24 13.771439602571652 15.072660673311649 54.61487521717563 16.541024506941067 25 12.38053813127549 19.663466355188145 52.9722821584875 14.983713355048861 26 12.563319983090235 14.992999064098273 56.44073884920742 16.002942103604077 27 13.723544892737843 16.188900645356778 54.17991305644001 15.907641405465373 28 11.780876815295029 15.918882000630452 57.590211885218864 14.710029298855659 29 14.432435470431127 13.668074999205828 55.95421656717111 15.94527296319194 30 12.960406225334836 17.11820463259137 56.89500551033524 13.026383631738552 31 16.0051413504842 15.155498972462986 52.60134251803993 16.23801715901288 32 15.649840798961955 17.657264478986196 51.30965151711381 15.383243204938044 33 14.393093387353353 15.970197761166677 50.96045998470302 18.676248866776955 34 15.538656651133467 17.89893727503537 51.01617423899949 15.546231834831673 35 14.103770242234825 19.210177137118155 47.29578160012316 19.390271020523862 36 19.631699455808576 18.569218851944257 44.89738069696577 16.901700995281395 37 15.581908506442572 20.5649132152745 45.87775608397213 17.97542219431079 38 18.892263782558018 19.961097766298252 44.46021928935002 16.686419161793705 39 17.438072873267178 20.072526274891196 44.44384711813132 18.0455537337103 40 16.765836410242628 20.189086359537768 45.04179790876058 18.00327932145903 41 19.107789976810164 21.334160901788966 43.04634790619479 16.51170121520608 42 16.051081174202345 21.177525651771248 43.580031815771534 19.191361358254873 43 19.319895120359895 19.94863536731088 42.02907404375524 18.702395468573986 44 18.412583601926542 20.771398061241694 42.07770183588242 18.73831650094934 45 16.60920116022491 20.750627396262747 41.1310482343713 21.50912320914105 46 18.765440545804204 22.259555116792225 39.72719564255885 19.24780869484472 47 15.233694417089612 20.007281950780857 46.337887403446956 18.421136228682577 48 17.35645637793813 21.6415667434774 40.460033575169035 20.54194330341543 49 15.322153013823488 20.110157832617766 43.03046445650501 21.53722469705374 50 17.420478898226186 19.331624437053886 42.068416126833014 21.179480537886917 51 16.69374998472745 19.780026439834714 40.89133032443779 22.634893251000047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 80.0 1 406.0 2 732.0 3 23338.0 4 45944.0 5 31529.5 6 17115.0 7 16288.5 8 15462.0 9 15070.0 10 14678.0 11 13776.5 12 12875.0 13 11975.5 14 11076.0 15 10269.5 16 9463.0 17 8742.0 18 8021.0 19 7316.5 20 6612.0 21 6171.0 22 5730.0 23 5414.5 24 5099.0 25 4910.5 26 4714.0 27 4706.0 28 4983.0 29 5260.0 30 5447.5 31 5635.0 32 6120.0 33 6605.0 34 7250.0 35 7895.0 36 8282.5 37 8670.0 38 9093.5 39 9517.0 40 9968.5 41 10420.0 42 11324.5 43 12229.0 44 13418.0 45 14607.0 46 27388.5 47 40170.0 48 32537.0 49 24904.0 50 22799.5 51 20695.0 52 17732.0 53 14769.0 54 13614.0 55 12459.0 56 11709.0 57 10959.0 58 10492.0 59 10025.0 60 9162.0 61 8299.0 62 7437.5 63 6576.0 64 5852.0 65 5128.0 66 4581.0 67 4034.0 68 3415.0 69 2796.0 70 2403.0 71 2010.0 72 1682.0 73 1354.0 74 1135.0 75 708.0 76 500.0 77 364.5 78 229.0 79 184.0 80 139.0 81 98.5 82 58.0 83 44.5 84 31.0 85 20.0 86 9.0 87 9.5 88 10.0 89 6.5 90 3.0 91 2.5 92 2.0 93 2.0 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 409231.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.4783552505026 #Duplication Level Percentage of deduplicated Percentage of total 1 82.68270206758471 41.736868080390074 2 7.0417197926461075 7.109088665353716 3 3.0680248373500416 4.646065429713626 4 1.8973344995427446 3.8309729958781293 5 1.3763059965099884 3.47368315126141 6 1.010963041183903 3.0619050922805724 7 0.7079732129727627 2.5016126346595198 8 0.522339729188071 2.1093480329125422 9 0.3899275291610675 1.7714610305048772 >10 1.2058741278215008 9.137532453942477 >50 0.04989975392832102 1.717312156860836 >100 0.03952455756698521 3.7932497684944 >500 0.00395245575669852 1.2666611159883667 >1k 0.00197622787834926 2.2455240575623656 >5k 9.8811393917463E-4 3.363298249698585 >10k+ 4.94056969587315E-4 8.235417084498492 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 33022 8.069281163939193 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCG 7678 1.8762019495101787 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGC 5808 1.4192473199733158 No Hit GAATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC 4753 1.1614467134698985 No Hit GAACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCT 1494 0.365074982100574 No Hit GCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTCTGC 1382 0.33770657648125385 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTTGCTTCGT 1375 0.3359960511300464 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTC 998 0.2438720429292991 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTA 763 0.18644726328161845 No Hit GAATGATACCTGTCTCTTCTACACATCTGACGCTTGCTTCGTCGTATGCCG 632 0.1544360031375922 No Hit GAATGACTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCT 583 0.14246232567913966 No Hit GAATGCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTT 552 0.134887141980935 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA 547 0.1336653381586439 No Hit GAATCTTTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCCGTCTTC 503 0.1229134645224824 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 501 0.122424742993566 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTGCTTCGTCGTATGCC 444 0.10849617941944771 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT 438 0.10703001483269842 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA 420 0.10263152107245052 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0896804005561651 0.0 2 0.0 0.0 0.0 0.5630072013117285 0.0 3 0.0 0.0 0.0 0.7455446923620156 0.0 4 0.0 0.0 0.0 1.3586458503876784 0.0 5 0.0 0.0 0.0 2.936483306494376 0.0 6 0.0 0.0 0.0 3.366069530411919 0.0 7 0.0 0.0 0.0 3.743362550735404 0.0 8 0.0 0.0 0.0 4.26433970056032 0.0 9 0.0 0.0 0.0 4.48328694551488 0.0 10 0.0 0.0 0.0 6.9984922940832925 0.0 11 0.0 0.0 0.0 7.515315310912419 0.0 12 0.0 0.0 0.0 9.500257800606503 0.0 13 0.0 0.0 0.0 9.709919336511652 0.0 14 0.0 0.0 0.0 9.851648579897418 0.0 15 0.0 0.0 0.0 10.199129586957 0.0 16 0.0 0.0 0.0 10.425896376374224 0.0 17 0.0 0.0 0.0 10.627982728581168 0.0 18 0.0 0.0 0.0 10.831046523845945 0.0 19 0.0 0.0 0.0 11.41506875090108 0.0 20 0.0 0.0 0.0 11.620331793045981 0.0 21 0.0 0.0 0.0 11.832681297360171 0.0 22 0.0 0.0 0.0 12.138620974461857 0.0 23 0.0 0.0 0.0 12.342417852020008 0.0 24 0.0 0.0 0.0 12.535462855941999 0.0 25 0.0 0.0 0.0 12.694297352839838 0.0 26 0.0 0.0 0.0 12.851665685150929 0.0 27 0.0 0.0 0.0 13.012699428928894 0.0 28 0.0 0.0 0.0 13.171778286591191 0.0 29 0.0 0.0 0.0 13.335744359542655 0.0 30 0.0 0.0 0.0 13.512661553010403 0.0 31 0.0 0.0 0.0 13.685424613482361 0.0 32 0.0 0.0 0.0 13.83057490757054 0.0 33 0.0 0.0 0.0 13.990142486761755 0.0 34 0.0 0.0 0.0 14.162172464940339 0.0 35 0.0 0.0 0.0 14.343488152168336 0.0 36 0.0 0.0 0.0 14.514051965760169 0.0 37 0.0 0.0 0.0 14.683393975529713 0.0 38 0.0 0.0 0.0 14.872529207220373 0.0 39 0.0 0.0 0.0 15.122754630025584 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTCA 30 2.1620617E-6 45.000004 16 CACCGGT 165 0.0 45.000004 16 TGATCCG 85 0.0 45.0 4 CGTTGAT 25 3.886388E-5 45.0 25 GGGTACA 50 2.1827873E-11 45.0 7 CGATTGC 20 7.0279563E-4 45.0 10 AGTTGAC 20 7.0279563E-4 45.0 32 CGCAACG 20 7.0279563E-4 45.0 16 TATACGG 50 2.1827873E-11 45.0 27 TATCGGG 25 3.886388E-5 45.0 3 TCGCGCG 20 7.0279563E-4 45.0 1 TAATCGT 25 3.886388E-5 45.0 21 CGTTTTT 14915 0.0 44.577602 1 CCGATGA 140 0.0 43.392857 18 CGATGAA 135 0.0 43.333332 19 TGATACC 1000 0.0 42.3 4 ACGGCTG 825 0.0 42.0 8 ACCCTTG 70 0.0 41.785713 39 GAATGAT 3240 0.0 41.52778 1 TACGGCT 835 0.0 41.497005 7 >>END_MODULE