FastQCFastQC Report
Sat 18 Jun 2016
SRR3549536_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549536_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences655249
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG242673.703477609275253No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC204413.1195774430788905No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC198723.0327402254715383RNA PCR Primer, Index 29 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188722.8801264862670526No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCT49710.7586428975854981No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC47720.7282727634838054No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTA45100.6882879638122301No Hit
GAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT44900.6852356890281405TruSeq Adapter, Index 12 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC41860.6388411123099769Illumina PCR Primer Index 8 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT40730.6215957597798699No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGT16100.24570812011922186No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT10850.1655859070368669No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATG10470.15978658494709644No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT10430.1591761299902785RNA PCR Primer, Index 29 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT6700.10225120526700536RNA PCR Primer, Index 29 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAT207.0310646E-445.08
TAGGGCG502.1827873E-1145.05
CGCGTCC207.0310646E-445.021
CTGCGAT302.164068E-644.99999631
CACTCGA302.164068E-644.99999644
TGCGATC302.164068E-644.99999632
ACTCGAG302.164068E-644.99999645
TAATGCG302.164068E-644.99999624
CGTTTTT148250.044.3929141
TACGGCT26500.042.452837
TGATACC28850.042.348354
GATACCT28800.042.265625
ACGGCTG26600.042.208658
CACCGGT2300.042.0652216
CCGATGA5950.041.9747918
CGGCTGT27400.041.4689759
CGATGAA6250.041.0419
CGACCAC21950.041.0022812
CACCCGT1650.040.9090916
ATACCTG29850.040.854276
Produced by FastQC (version 0.11.5)