##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549536_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	655249
Sequences flagged as poor quality	0
Sequence length	51
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.139760610088683	31.0	28.0	31.0	25.0	34.0
2	28.064659389026158	31.0	25.0	33.0	16.0	34.0
3	28.046208387956334	31.0	25.0	33.0	16.0	34.0
4	32.83959685554652	35.0	32.0	37.0	28.0	37.0
5	34.120604533543734	35.0	33.0	37.0	30.0	37.0
6	33.22198278822249	35.0	32.0	37.0	28.0	37.0
7	33.472661537827605	35.0	32.0	37.0	29.0	37.0
8	33.195565349966195	35.0	32.0	37.0	28.0	37.0
9	34.53819998199158	37.0	34.0	39.0	28.0	39.0
10	34.476563871139064	37.0	32.0	39.0	27.0	39.0
11	34.68020706632135	37.0	33.0	39.0	27.0	39.0
12	34.748355205425725	37.0	34.0	39.0	27.0	39.0
13	34.81059566668549	37.0	33.0	39.0	27.0	39.0
14	35.62419782403331	38.0	34.0	40.0	27.0	41.0
15	35.84641411127678	38.0	34.0	40.0	27.0	41.0
16	35.86275293819602	38.0	34.0	40.0	29.0	41.0
17	35.12424437122377	37.0	33.0	40.0	27.0	41.0
18	34.96450204426104	37.0	33.0	39.0	27.0	40.0
19	34.32281773798968	37.0	32.0	38.0	27.0	40.0
20	33.53985584106195	35.0	32.0	38.0	25.0	40.0
21	34.14822456806496	35.0	33.0	38.0	27.0	40.0
22	34.71189578312977	35.0	33.0	39.0	28.0	40.0
23	34.95785876819347	35.0	33.0	39.0	29.0	40.0
24	34.582019964929366	35.0	33.0	39.0	27.0	40.0
25	33.5609867393922	35.0	32.0	38.0	24.0	40.0
26	33.6312211083115	35.0	32.0	38.0	24.0	40.0
27	34.31517789420511	35.0	33.0	38.0	27.0	40.0
28	33.957983911459614	35.0	33.0	39.0	24.0	40.0
29	33.980239573047804	35.0	33.0	39.0	24.0	40.0
30	33.35981436064763	35.0	32.0	39.0	22.0	40.0
31	33.51953532168687	35.0	33.0	39.0	23.0	40.0
32	32.929066660155144	35.0	32.0	39.0	19.0	40.0
33	32.34752895464167	35.0	31.0	39.0	15.0	40.0
34	32.02967574158831	35.0	31.0	39.0	12.0	40.0
35	31.573864286706275	35.0	30.0	39.0	10.0	40.0
36	31.21740590218375	35.0	29.0	40.0	8.0	40.0
37	31.08244041578087	35.0	28.0	40.0	8.0	40.0
38	30.799563219478397	35.0	26.0	39.0	8.0	40.0
39	30.468676793096975	35.0	25.0	39.0	7.0	40.0
40	30.16649090651035	35.0	24.0	39.0	8.0	40.0
41	29.70949059059991	35.0	23.0	39.0	7.0	40.0
42	30.02333769299915	35.0	23.0	39.0	7.0	40.0
43	30.006325839490025	35.0	23.0	39.0	7.0	40.0
44	30.040723450169324	35.0	23.0	39.0	7.0	40.0
45	30.20895873171878	35.0	23.0	39.0	7.0	40.0
46	29.94424257038164	35.0	23.0	39.0	7.0	40.0
47	29.86857210007188	35.0	23.0	39.0	7.0	40.0
48	29.738615396589694	35.0	23.0	38.0	7.0	40.0
49	29.988187696585573	35.0	24.0	39.0	7.0	40.0
50	29.93495602435105	35.0	24.0	39.0	7.0	40.0
51	28.805132094821968	34.0	22.0	38.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	19.0
9	35.0
10	40.0
11	57.0
12	70.0
13	86.0
14	127.0
15	175.0
16	308.0
17	603.0
18	1264.0
19	2363.0
20	3815.0
21	5818.0
22	7962.0
23	11236.0
24	15684.0
25	22414.0
26	28957.0
27	32189.0
28	30998.0
29	29079.0
30	30951.0
31	35520.0
32	41373.0
33	48723.0
34	55057.0
35	62946.0
36	66645.0
37	65032.0
38	48640.0
39	7061.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.00615338596472	2.4434985784030196	29.986921002550176	35.56342703308208
2	35.01584893681638	21.36836530845526	30.250790157634732	13.364995597093623
3	12.087465986212875	21.77630183334885	52.93697510412072	13.199257076317553
4	10.816803993596327	2.9219426508090818	70.7431831258041	15.518070229790506
5	25.012018331962356	3.382683529467424	54.15864808645263	17.446650052117594
6	13.387735044235091	18.783851635027297	57.334539999297974	10.493873321439635
7	50.12476173179967	1.5754316298079052	45.25562038248056	3.0441862559118746
8	48.18580417520668	14.762326993249895	32.92092013875641	4.130948692787017
9	45.415712194906064	3.1267502888215013	31.542055005043885	19.915482511228554
10	30.31229349453414	18.21139749927127	37.8743042721164	13.602004734078191
11	26.82186466518835	14.970186906046404	43.853863187887356	14.354085240877895
12	19.59865638864004	13.317380110461825	46.73643149398168	20.347532006916456
13	18.56897149022738	14.99628385545037	54.3687972053372	12.065947448985042
14	15.874881152050593	22.147611060833363	44.00296681109013	17.974540976025907
15	11.714020166379498	14.81757316684192	57.19672979279633	16.27167687398226
16	13.634969301746358	17.375989890865913	44.30254758114854	24.686493226239186
17	14.374077640713685	18.141042565498005	50.525372797211446	16.959506996576874
18	14.416046418994915	16.901819003157577	48.64562937142979	20.03650520641771
19	14.117839172589353	19.692513838250804	44.92414334092841	21.265503648231434
20	16.7698081187457	18.80338619364547	51.6194606935684	12.807344994040434
21	16.80964030467807	24.689240273544865	45.10453278066811	13.396586641108952
22	14.390865152026178	14.546073324797138	49.588934893452716	21.474126629723965
23	14.868546155736217	23.560356444649287	47.153982684445154	14.41711471516935
24	17.259698221592096	17.515784075977223	44.41258208711498	20.811935615315704
25	13.590253476159445	27.573029489552823	42.280415536689105	16.55630149759862
26	14.388423332198904	15.764541418605752	47.91644092551076	21.930594323684584
27	17.34790896285229	19.648103240142298	43.29773872222621	19.706249074779205
28	11.31875058183988	19.493963363545767	49.1968701974364	19.99041585717796
29	17.408649231055676	16.671830098176418	43.73925026974479	22.180270401023122
30	14.142257370862069	26.000192293311397	44.870423304728426	14.987127031098101
31	17.58568116853288	16.756225495956496	40.98991375797598	24.668179577534648
32	19.43520707395204	21.912585902458453	42.5795384655299	16.072668558059608
33	15.704106377880775	22.007511648243643	37.5306181314279	24.75776384244768
34	18.231084671628647	22.39515054582304	38.28453000309806	21.089234779450255
35	14.544547187405094	25.775239641723985	36.58456556209929	23.09564760877163
36	16.096781528853917	22.186832791808918	32.81622711366213	28.90015856567503
37	15.64641838446148	25.777223620333643	41.03676617591175	17.539591819293125
38	17.915174231475362	25.6873341279422	31.991502467001094	24.40598917358134
39	18.994153367651077	25.37447596257301	36.870411095629294	18.76095957414662
40	16.830243159470676	25.37142368778892	35.42409068918839	22.374242463552026
41	16.791784497191145	27.39920244059892	31.797377790732988	24.01163527147695
42	16.689685905663342	21.246426930830875	39.3949475695499	22.668939593955887
43	19.12799561693341	20.79621640017764	32.71733341065763	27.358454572231324
44	20.252606261131266	23.01781460177734	34.74923273442615	21.980346402665248
45	16.931426068563248	20.648944141845313	34.78875969288011	27.630870096711323
46	23.022698241431883	23.48191298269818	32.96166800712401	20.533720768745926
47	14.97995418535549	22.091907046023724	43.221431852623965	19.70670691599682
48	19.330361435118558	24.948378402714084	32.248046162603835	23.473213999563526
49	17.278622325253455	19.674505417024672	40.23844370613309	22.808428551588786
50	18.787972205985817	19.468782096577026	36.687427222323116	25.055818475114037
51	18.6989983960296	20.14806584977619	34.20440168546614	26.94853406872807
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	83.0
1	976.5
2	1870.0
3	20997.5
4	40125.0
5	30000.5
6	19876.0
7	18762.5
8	17649.0
9	17010.5
10	16372.0
11	15548.0
12	14724.0
13	13631.5
14	12539.0
15	11492.5
16	10446.0
17	9397.0
18	8348.0
19	7882.0
20	7416.0
21	6818.5
22	6221.0
23	6003.5
24	5786.0
25	5773.5
26	6137.0
27	6513.0
28	6884.5
29	7256.0
30	7868.0
31	8480.0
32	9337.0
33	10194.0
34	11244.5
35	12295.0
36	13264.5
37	14234.0
38	15059.0
39	15884.0
40	17144.0
41	18404.0
42	19845.5
43	21287.0
44	24376.0
45	27465.0
46	73514.0
47	119563.0
48	82652.0
49	45741.0
50	41018.5
51	36296.0
52	31947.0
53	27598.0
54	26063.5
55	24529.0
56	23353.5
57	22178.0
58	20847.5
59	19517.0
60	17647.5
61	15778.0
62	13707.5
63	11637.0
64	9814.5
65	7992.0
66	6459.0
67	4926.0
68	4219.5
69	3513.0
70	3021.0
71	2529.0
72	2077.5
73	1626.0
74	1377.5
75	923.5
76	718.0
77	561.5
78	405.0
79	304.0
80	203.0
81	138.5
82	74.0
83	58.5
84	43.0
85	30.0
86	17.0
87	9.5
88	2.0
89	1.5
90	1.0
91	2.0
92	3.0
93	2.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	655249.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.29870896933052
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.66970163837686	33.2740996320662
2	5.4244813643845875	4.263502288970137
3	1.9301503761775491	2.2755725370133595
4	1.105050208942001	1.737081862308383
5	0.8026694146745367	1.5771935862938746
6	0.6212483161186065	1.4648554063699062
7	0.5483899614440277	1.5085712247543863
8	0.4753080595104062	1.4943194481181348
9	0.43647832563092087	1.5437731221351214
>10	3.8355517545720628	26.395032897306564
>50	0.09514291771745492	2.67245356781139
>100	0.048357763954031165	3.515117714758941
>500	0.00196576276235899	0.47355438423524926
>1k	0.00393152552471798	4.9112147509578685
>5k	0.0	0.0
>10k+	0.001572610209887192	12.893657576900496
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	24267	3.703477609275253	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	20441	3.1195774430788905	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	19872	3.0327402254715383	RNA PCR Primer, Index 29 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18872	2.8801264862670526	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTAACCT	4971	0.7586428975854981	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTC	4772	0.7282727634838054	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTA	4510	0.6882879638122301	No Hit
GAACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCT	4490	0.6852356890281405	TruSeq Adapter, Index 12 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	4186	0.6388411123099769	Illumina PCR Primer Index 8 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCT	4073	0.6215957597798699	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCACTAACCTCGT	1610	0.24570812011922186	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTAT	1085	0.1655859070368669	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATG	1047	0.15978658494709644	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTT	1043	0.1591761299902785	RNA PCR Primer, Index 29 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	670	0.10225120526700536	RNA PCR Primer, Index 29 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.526137392044856E-4	0.0	0.0	0.11156064335847898	0.0
2	1.526137392044856E-4	0.0	0.0	0.880276047731473	0.0
3	1.526137392044856E-4	0.0	0.0	0.9964151032660866	0.0
4	1.526137392044856E-4	0.0	0.0	1.842505673415755	0.0
5	1.526137392044856E-4	0.0	0.0	5.469981640567173	0.0
6	1.526137392044856E-4	0.0	0.0	5.792149244027843	0.0
7	1.526137392044856E-4	0.0	0.0	6.603291267899684	0.0
8	1.526137392044856E-4	0.0	0.0	7.545833721226587	0.0
9	1.526137392044856E-4	0.0	0.0	7.682880859032215	0.0
10	1.526137392044856E-4	0.0	0.0	12.169877405383296	0.0
11	1.526137392044856E-4	0.0	0.0	12.479835909707607	0.0
12	1.526137392044856E-4	0.0	0.0	16.405366509525386	0.0
13	1.526137392044856E-4	0.0	0.0	16.75179969751957	0.0
14	1.526137392044856E-4	0.0	0.0	16.995981680246746	0.0
15	1.526137392044856E-4	0.0	0.0	17.90723831703673	0.0
16	1.526137392044856E-4	0.0	0.0	18.311817339667822	0.0
17	1.526137392044856E-4	0.0	0.0	18.503194968630247	0.0
18	1.526137392044856E-4	0.0	0.0	18.642378698784736	0.0
19	1.526137392044856E-4	0.0	0.0	19.706249074779205	0.0
20	1.526137392044856E-4	0.0	0.0	19.947531396461496	0.0
21	1.526137392044856E-4	0.0	0.0	20.102892182971665	0.0
22	1.526137392044856E-4	0.0	0.0	20.422770580344267	0.0
23	1.526137392044856E-4	0.0	0.0	20.568821928762958	0.0
24	1.526137392044856E-4	0.0	0.0	20.710447478744722	0.0
25	1.526137392044856E-4	0.0	0.0	20.832080628890697	0.0
26	1.526137392044856E-4	0.0	0.0	20.977368908613368	0.0
27	1.526137392044856E-4	0.0	0.0	21.178055975667267	0.0
28	1.526137392044856E-4	0.0	0.0	21.303351855554148	0.0
29	1.526137392044856E-4	0.0	0.0	21.440551607098982	0.0
30	1.526137392044856E-4	0.0	0.0	21.60415353552619	0.0
31	3.052274784089712E-4	0.0	0.0	21.745626471768748	0.0
32	3.052274784089712E-4	0.0	0.0	21.90220816819255	0.0
33	3.052274784089712E-4	0.0	0.0	22.062757821835667	0.0
34	3.052274784089712E-4	0.0	0.0	22.27641705672195	0.0
35	3.052274784089712E-4	0.0	0.0	22.46153752237699	0.0
36	3.052274784089712E-4	0.0	0.0	22.620561038628065	0.0
37	3.052274784089712E-4	0.0	0.0	22.787062628100156	0.0
38	3.052274784089712E-4	0.0	0.0	22.983934351673945	0.0
39	3.052274784089712E-4	0.0	0.0	23.245972141888046	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCGTAT	20	7.0310646E-4	45.0	8
TAGGGCG	50	2.1827873E-11	45.0	5
CGCGTCC	20	7.0310646E-4	45.0	21
CTGCGAT	30	2.164068E-6	44.999996	31
CACTCGA	30	2.164068E-6	44.999996	44
TGCGATC	30	2.164068E-6	44.999996	32
ACTCGAG	30	2.164068E-6	44.999996	45
TAATGCG	30	2.164068E-6	44.999996	24
CGTTTTT	14825	0.0	44.392914	1
TACGGCT	2650	0.0	42.45283	7
TGATACC	2885	0.0	42.34835	4
GATACCT	2880	0.0	42.26562	5
ACGGCTG	2660	0.0	42.20865	8
CACCGGT	230	0.0	42.06522	16
CCGATGA	595	0.0	41.97479	18
CGGCTGT	2740	0.0	41.468975	9
CGATGAA	625	0.0	41.04	19
CGACCAC	2195	0.0	41.00228	12
CACCCGT	165	0.0	40.90909	16
ATACCTG	2985	0.0	40.85427	6
>>END_MODULE