SRR3549534_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549534_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	702839
Sequences flagged as poor quality	0
Sequence length	51
%GC	35

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20923	2.9769264369222537	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	19914	2.8333658206217924	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	19572	2.7847060279808034	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	14853	2.1132862575924216	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	4576	0.6510737167402492	TruSeq Adapter, Index 7 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT	3909	0.5561728930807767	No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	3684	0.5241598716064418	Illumina PCR Primer Index 3 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC	3591	0.5109278227303835	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	3217	0.45771506703526693	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA	2642	0.3759040121564114	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTT	2405	0.34218362953677867	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGT	1237	0.17600047806112068	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATACGC	25	3.889254E-5	45.0	5
CGAACGA	20	7.0314144E-4	45.0	22
ACGATCA	20	7.0314144E-4	45.0	37
ATAACGA	20	7.0314144E-4	45.0	18
CGCCGAT	20	7.0314144E-4	45.0	16
TAATGCG	20	7.0314144E-4	45.0	13
CGTTTTT	23000	0.0	44.43261	1
TACGGCT	1870	0.0	43.676468	7
CCGATGA	610	0.0	43.15574	18
ACGGCTG	1895	0.0	42.862797	8
CGATGAA	635	0.0	42.519684	19
CGGCTGT	1925	0.0	42.428574	9
GATACCT	2305	0.0	42.266815	5
TGATACC	2320	0.0	42.09052	4
CACCGGT	125	0.0	41.399998	16
CGACCAC	1750	0.0	40.885715	12
ATACCTG	2400	0.0	40.6875	6
GAATCTG	2145	0.0	40.384613	1
GGCGACT	410	0.0	40.060974	10
CCGGGTA	45	1.9264917E-8	40.0	5