##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549534_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 702839 Sequences flagged as poor quality 0 Sequence length 51 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.476890155497916 31.0 28.0 33.0 25.0 34.0 2 28.64004558654258 31.0 27.0 33.0 16.0 34.0 3 28.600658187721514 31.0 27.0 34.0 16.0 34.0 4 33.210188962194756 35.0 32.0 37.0 28.0 37.0 5 34.315214437445846 35.0 33.0 37.0 30.0 37.0 6 33.74476089118561 35.0 33.0 37.0 28.0 37.0 7 33.84411223623049 35.0 33.0 37.0 29.0 37.0 8 33.6595806436467 35.0 33.0 37.0 28.0 37.0 9 34.95781679730351 37.0 34.0 39.0 28.0 39.0 10 34.87145135656957 37.0 34.0 39.0 28.0 39.0 11 35.24259325393155 37.0 34.0 39.0 30.0 39.0 12 35.37715607699629 37.0 34.0 39.0 30.0 39.0 13 35.549488574196936 37.0 34.0 39.0 30.0 39.0 14 36.40319048886018 38.0 35.0 40.0 30.0 41.0 15 36.65413842999606 38.0 35.0 41.0 30.0 41.0 16 36.60042342556403 38.0 34.0 41.0 30.0 41.0 17 35.83325911055021 38.0 34.0 40.0 27.0 41.0 18 35.55446553193548 38.0 34.0 39.0 28.0 40.0 19 34.81714731254242 37.0 34.0 38.0 27.0 40.0 20 33.84530312062933 35.0 33.0 37.0 27.0 40.0 21 34.23443206765703 35.0 33.0 38.0 27.0 40.0 22 34.63974395274025 35.0 33.0 38.0 29.0 40.0 23 34.931264485892214 35.0 33.0 39.0 30.0 40.0 24 34.38204766667757 35.0 33.0 38.0 27.0 40.0 25 33.448761380629136 35.0 33.0 38.0 24.0 40.0 26 33.3463808923523 35.0 32.0 38.0 24.0 40.0 27 34.14596230431152 35.0 33.0 38.0 26.0 40.0 28 33.818191648442955 35.0 33.0 38.0 24.0 40.0 29 33.73927741630729 35.0 33.0 39.0 23.0 40.0 30 32.93623148402408 35.0 32.0 38.0 21.0 40.0 31 32.71516378573187 35.0 32.0 38.0 20.0 40.0 32 31.872474350455793 35.0 31.0 38.0 15.0 40.0 33 30.778959334925922 35.0 26.0 39.0 9.0 40.0 34 29.87550207088679 35.0 22.0 39.0 7.0 40.0 35 29.213040255307405 35.0 18.0 39.0 7.0 40.0 36 28.64055210368235 35.0 16.0 39.0 7.0 40.0 37 28.4499266546108 35.0 15.0 39.0 7.0 40.0 38 28.31525143027066 35.0 15.0 39.0 7.0 40.0 39 27.91534903441613 35.0 15.0 39.0 7.0 40.0 40 27.822189719124864 35.0 15.0 39.0 7.0 40.0 41 27.32917353760961 33.0 15.0 39.0 7.0 40.0 42 27.513884403113657 34.0 13.0 39.0 7.0 40.0 43 27.48910917009443 34.0 12.0 39.0 7.0 40.0 44 27.494498740109755 34.0 12.0 39.0 7.0 40.0 45 27.646081108191208 35.0 12.0 39.0 7.0 40.0 46 27.403069550779055 34.0 10.0 39.0 7.0 40.0 47 27.33267078235556 34.0 10.0 39.0 7.0 40.0 48 27.156997548513957 34.0 10.0 38.0 7.0 40.0 49 27.355621984551227 34.0 10.0 39.0 7.0 40.0 50 27.358457626853376 34.0 10.0 39.0 7.0 40.0 51 26.189218298927635 32.0 10.0 37.0 7.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 11.0 9 21.0 10 43.0 11 49.0 12 67.0 13 81.0 14 151.0 15 204.0 16 412.0 17 863.0 18 1676.0 19 3280.0 20 5368.0 21 8119.0 22 12122.0 23 17968.0 24 26508.0 25 38037.0 26 46518.0 27 43146.0 28 34731.0 29 29454.0 30 28270.0 31 30855.0 32 35226.0 33 42162.0 34 49045.0 35 58537.0 36 64796.0 37 66944.0 38 50763.0 39 7409.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.55179778014595 2.142880517444251 28.117534741242306 44.18778696116749 2 43.236644523141145 16.5322356898237 27.42847223901918 12.802647548015974 3 9.852042928750397 16.65658849323956 60.48497593332186 13.006392644688185 4 8.740550823161493 2.244041665303149 74.63032074201915 14.385086769516207 5 19.46192513505938 2.816576769359697 62.76188430067199 14.959613794908933 6 11.100550766249453 13.875297187549354 65.60705936921542 9.417092676985767 7 45.43359147685317 0.9325037455235126 51.35571588941422 2.2781888882091064 8 44.437915368953625 10.321994083993632 42.17978797420177 3.060302572850966 9 41.745122282628024 2.1569662468929582 41.156082687500266 14.941828782978748 10 25.158393316250237 12.340948638308348 49.613496120733195 12.887161924708218 11 20.888994492337506 14.316080923227084 51.45488511593693 13.340039468498475 12 14.521960221330914 12.690815392998967 55.82060756446355 16.966616821206564 13 14.821744382426132 13.746675981270249 61.21529966322301 10.216279973080605 14 13.410183555551129 19.188035951334516 53.33611253786429 14.065667955250063 15 10.46612382067586 14.279657218794062 61.82127058970831 13.432948370821768 16 11.660849782098035 16.201292187826798 52.31254953125822 19.82530849881694 17 12.193261899240081 16.927489794960156 56.80418986425057 14.075058441549201 18 12.864254829342139 15.489749430523919 55.25874346756512 16.387252272568826 19 12.659229211810954 18.09532481834389 51.921421548889576 17.32402442095558 20 14.651008267896346 16.666974940206792 57.44302749278284 11.23898929911402 21 14.407851584786844 20.844887662750644 53.35347070950816 11.393790042954361 22 12.987753952185352 13.268472580491409 55.814916360645896 17.92885710667735 23 13.321258495900198 19.727277513057757 54.40221729300736 12.549246698034686 24 15.093357084623932 15.793375154196054 52.22248623084377 16.890781530336252 25 12.009151455738797 23.805594168792567 50.37156446924544 13.813689906223189 26 12.583678481131525 14.75444589728231 55.75416275989238 16.90771286169379 27 15.895959103009366 17.14631658174916 51.00158073186035 15.956143583381117 28 12.126959374764349 16.856634307430294 55.81946932370002 15.196936994105336 29 15.397125088391508 15.05764478066812 54.43394575429081 15.111284376649559 30 13.099301547011477 21.83999465026841 51.92099470860325 13.139709094116862 31 17.75783643195668 15.821831173284352 49.17584254715518 17.244489847603788 32 16.285237444137277 18.817965423091206 49.831042386663235 15.065754746108285 33 14.302422034064701 17.758263272243003 43.12964989136915 24.80966480232315 34 16.86787443497017 21.717633768188733 45.1027902549517 16.311701541889395 35 14.165121741963665 23.450747610761496 39.4054683931882 22.97866225408664 36 19.066244189636603 20.437682029597106 38.59148396716744 21.904589813598847 37 15.55960895738569 24.735536872598136 42.2395456142872 17.465308555728978 38 20.519066244189638 22.226569669582936 35.06208391964589 22.192280166581536 39 19.537760425929694 24.50006331464247 38.067893215942775 17.894283043485064 40 18.30291147759302 22.16866167073825 37.120734620588784 22.40769223107995 41 18.201181209352356 26.765446994261843 34.19175657583031 20.84161522055549 42 17.550249772707545 22.897562599684992 39.54177272462115 20.01041490298632 43 19.957486707482083 21.271585668979668 35.52719755164412 23.24373007189413 44 20.537989496883355 23.20431848545684 34.9441337205249 21.313558297134904 45 18.407629627837956 21.832169245019127 35.67986409405283 24.080337033090082 46 22.15912890434367 23.544367913562 34.517008874009555 19.779494308084782 47 16.554431384712572 21.492262097009416 41.7923592743146 20.160947243963413 48 19.608046793077786 24.53919034088888 33.30364422008454 22.549118645948788 49 17.28005987146416 21.653180884953738 38.98133142867712 22.08542781490498 50 18.70954799036479 20.71142893322653 36.76802226398934 23.811000812419344 51 18.78894028362114 21.410024201844237 34.7184774891547 25.082558025379925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 47.0 1 928.5 2 1810.0 3 22190.5 4 42571.0 5 32884.5 6 23198.0 7 22574.5 8 21951.0 9 21861.5 10 21772.0 11 21589.5 12 21407.0 13 20750.5 14 20094.0 15 19259.5 16 18425.0 17 17699.5 18 16974.0 19 16001.0 20 15028.0 21 14135.0 22 13242.0 23 12602.5 24 11963.0 25 11507.0 26 10723.5 27 10396.0 28 10279.0 29 10162.0 30 10602.5 31 11043.0 32 11363.0 33 11683.0 34 12638.0 35 13593.0 36 14611.5 37 15630.0 38 16535.0 39 17440.0 40 18632.0 41 19824.0 42 20802.5 43 21781.0 44 24458.5 45 27136.0 46 65941.5 47 104747.0 48 73259.0 49 41771.0 50 38677.0 51 35583.0 52 30876.0 53 26169.0 54 24805.5 55 23442.0 56 21532.0 57 19622.0 58 17624.0 59 15626.0 60 13783.5 61 11941.0 62 10289.5 63 8638.0 64 7211.5 65 5785.0 66 4908.0 67 4031.0 68 3322.5 69 2614.0 70 2152.0 71 1690.0 72 1453.5 73 1217.0 74 990.5 75 642.5 76 521.0 77 376.5 78 232.0 79 172.5 80 113.0 81 82.0 82 51.0 83 44.0 84 37.0 85 23.5 86 10.0 87 9.0 88 8.0 89 5.5 90 3.0 91 3.0 92 3.0 93 1.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 702839.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.83039349767193 #Duplication Level Percentage of deduplicated Percentage of total 1 83.17037359751477 45.60264311700119 2 7.277011376624326 7.980027945346943 3 3.5755341315730984 5.881438301955291 4 2.094267054203261 4.593179466847001 5 1.2941664512266968 3.547982788612272 6 0.8292854878887838 2.728202977371052 7 0.5318045741542895 2.041133784531908 8 0.3188558829549215 1.3986394825172777 9 0.203313718021846 1.0032994046351278 >10 0.6306505955201344 5.583467655619513 >50 0.03985982193820919 1.5326023571420904 >100 0.030419306556717714 3.2133005303706144 >500 0.0013111770067519468 0.4404125436062422 >1k 0.002097883210803115 3.6321453686050553 >5k 0.0 0.0 >10k+ 0.0010489416054015575 10.821524275838396 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20923 2.9769264369222537 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG 19914 2.8333658206217924 No Hit GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC 19572 2.7847060279808034 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC 14853 2.1132862575924216 No Hit GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT 4576 0.6510737167402492 TruSeq Adapter, Index 7 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGTCACCT 3909 0.5561728930807767 No Hit GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC 3684 0.5241598716064418 Illumina PCR Primer Index 3 (95% over 22bp) GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC 3591 0.5109278227303835 No Hit GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT 3217 0.45771506703526693 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA 2642 0.3759040121564114 No Hit GAATGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTT 2405 0.34218362953677867 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGT 1237 0.17600047806112068 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.06103816094439836 0.0 2 0.0 0.0 0.0 0.6554843996989353 0.0 3 0.0 0.0 0.0 0.7594911494666631 0.0 4 0.0 0.0 0.0 1.548718838880597 0.0 5 0.0 0.0 0.0 4.823152955371002 0.0 6 0.0 0.0 0.0 5.319283648175471 0.0 7 0.0 0.0 0.0 5.940193984682125 0.0 8 0.0 0.0 0.0 6.641065734826895 0.0 9 0.0 0.0 0.0 6.797004719430766 0.0 10 0.0 0.0 0.0 10.139448721542202 0.0 11 0.0 0.0 0.0 10.334087892106158 0.0 12 0.0 0.0 0.0 12.941370641071426 0.0 13 0.0 0.0 0.0 13.149668700797765 0.0 14 0.0 0.0 0.0 13.30119700244295 0.0 15 0.0 0.0 0.0 13.79746997534286 0.0 16 0.0 0.0 0.0 14.073635640594787 0.0 17 0.0 0.0 0.0 14.221464659758494 0.0 18 0.0 0.0 0.0 14.337280657447865 0.0 19 0.0 0.0 0.0 15.090653762810543 0.0 20 0.0 0.0 0.0 15.249580629418686 0.0 21 0.0 0.0 0.0 15.35330281899553 0.0 22 0.0 0.0 0.0 15.581804652274561 0.0 23 0.0 0.0 0.0 15.690222085000975 0.0 24 0.0 0.0 0.0 15.795651635723118 0.0 25 0.0 0.0 0.0 15.880592852701685 0.0 26 0.0 0.0 0.0 16.010494579839765 0.0 27 0.0 0.0 0.0 16.173405289120268 0.0 28 0.0 0.0 0.0 16.270867154497687 0.0 29 0.0 0.0 0.0 16.37715038579248 0.0 30 0.0 0.0 0.0 16.49652338586789 0.0 31 1.4228009544148802E-4 0.0 0.0 16.597399973535904 0.0 32 1.4228009544148802E-4 0.0 0.0 16.72260645752441 0.0 33 1.4228009544148802E-4 0.0 0.0 16.843402258554235 0.0 34 1.4228009544148802E-4 0.0 0.0 17.0102968105071 0.0 35 1.4228009544148802E-4 0.0 0.0 17.14802394289446 0.0 36 1.4228009544148802E-4 0.0 0.0 17.27692970936445 0.0 37 1.4228009544148802E-4 0.0 0.0 17.413518600988276 0.0 38 1.4228009544148802E-4 0.0 0.0 17.553806775093584 0.0 39 1.4228009544148802E-4 0.0 0.0 17.746027184035036 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATACGC 25 3.889254E-5 45.0 5 CGAACGA 20 7.0314144E-4 45.0 22 ACGATCA 20 7.0314144E-4 45.0 37 ATAACGA 20 7.0314144E-4 45.0 18 CGCCGAT 20 7.0314144E-4 45.0 16 TAATGCG 20 7.0314144E-4 45.0 13 CGTTTTT 23000 0.0 44.43261 1 TACGGCT 1870 0.0 43.676468 7 CCGATGA 610 0.0 43.15574 18 ACGGCTG 1895 0.0 42.862797 8 CGATGAA 635 0.0 42.519684 19 CGGCTGT 1925 0.0 42.428574 9 GATACCT 2305 0.0 42.266815 5 TGATACC 2320 0.0 42.09052 4 CACCGGT 125 0.0 41.399998 16 CGACCAC 1750 0.0 40.885715 12 ATACCTG 2400 0.0 40.6875 6 GAATCTG 2145 0.0 40.384613 1 GGCGACT 410 0.0 40.060974 10 CCGGGTA 45 1.9264917E-8 40.0 5 >>END_MODULE