##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549531_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	369849
Sequences flagged as poor quality	0
Sequence length	51
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.732317783744175	31.0	28.0	31.0	16.0	33.0
2	29.268563657060042	31.0	30.0	33.0	16.0	34.0
3	29.517822138223977	31.0	30.0	33.0	16.0	34.0
4	32.07801832639807	35.0	30.0	37.0	19.0	37.0
5	33.456545779493794	35.0	33.0	37.0	28.0	37.0
6	33.00167095219941	35.0	33.0	37.0	25.0	37.0
7	31.47395017966792	33.0	30.0	35.0	23.0	37.0
8	33.04217667210132	35.0	32.0	35.0	28.0	37.0
9	32.46883998604836	34.0	31.0	37.0	25.0	39.0
10	30.422588678082136	34.0	27.0	37.0	15.0	39.0
11	31.16367220135785	35.0	27.0	39.0	15.0	39.0
12	32.29419844314842	35.0	30.0	39.0	18.0	39.0
13	32.40190726485674	35.0	30.0	39.0	21.0	39.0
14	32.80689416491595	36.0	30.0	39.0	17.0	41.0
15	33.45085967516473	36.0	31.0	39.0	22.0	41.0
16	33.64219181341574	36.0	31.0	39.0	22.0	41.0
17	31.60906207668535	34.0	27.0	39.0	17.0	41.0
18	32.42963209309745	35.0	29.0	39.0	19.0	39.0
19	32.33491776373601	35.0	30.0	37.0	18.0	39.0
20	32.32154744233458	34.0	30.0	37.0	23.0	39.0
21	32.5885131499612	35.0	31.0	37.0	23.0	39.0
22	33.0050507098843	35.0	31.0	38.0	25.0	39.0
23	34.12414796308764	35.0	33.0	38.0	27.0	40.0
24	32.25530419171067	35.0	30.0	37.0	21.0	39.0
25	31.34338608459128	34.0	29.0	37.0	18.0	39.0
26	30.883160424930175	33.0	26.0	37.0	19.0	39.0
27	32.35715656930261	34.0	30.0	38.0	22.0	39.0
28	32.41692150039611	35.0	31.0	38.0	21.0	39.0
29	32.79568688843285	35.0	31.0	38.0	22.0	40.0
30	31.662670441180047	35.0	30.0	38.0	18.0	40.0
31	31.897742051485878	35.0	30.0	38.0	20.0	40.0
32	31.82126489459212	35.0	30.0	38.0	18.0	40.0
33	32.05220238529778	35.0	30.0	38.0	18.0	40.0
34	31.443864928660076	35.0	30.0	38.0	15.0	40.0
35	31.010496175466205	35.0	29.0	38.0	10.0	40.0
36	30.411792380133512	35.0	27.0	38.0	10.0	40.0
37	30.63846867235007	35.0	29.0	38.0	10.0	40.0
38	30.54143988492601	35.0	27.0	38.0	10.0	40.0
39	30.146440844777192	35.0	26.0	38.0	8.0	40.0
40	29.9283734713356	34.0	25.0	38.0	8.0	40.0
41	29.40477059556738	34.0	24.0	38.0	8.0	40.0
42	29.882230315615292	34.0	25.0	38.0	8.0	40.0
43	29.84756752079903	34.0	25.0	38.0	8.0	40.0
44	29.863995846953756	34.0	25.0	38.0	8.0	40.0
45	30.091180454726118	35.0	26.0	38.0	8.0	40.0
46	29.913388977663857	34.0	25.0	38.0	8.0	40.0
47	29.992450973235023	35.0	25.0	38.0	8.0	40.0
48	29.70150791268869	34.0	25.0	38.0	8.0	40.0
49	29.42467060881603	34.0	24.0	37.0	7.0	39.0
50	29.622213389788804	34.0	25.0	37.0	8.0	40.0
51	27.990009436283458	32.0	23.0	36.0	7.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	21.0
11	35.0
12	65.0
13	66.0
14	149.0
15	261.0
16	434.0
17	712.0
18	1336.0
19	1946.0
20	2840.0
21	4295.0
22	5652.0
23	7702.0
24	10416.0
25	14276.0
26	17186.0
27	19005.0
28	19506.0
29	21122.0
30	23928.0
31	27422.0
32	30983.0
33	34602.0
34	38292.0
35	40214.0
36	29224.0
37	15106.0
38	2985.0
39	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	20.54649329861646	2.8736051740034445	39.916560542275356	36.66334098510473
2	36.30130134189899	8.337186257094112	39.833824074149184	15.527688326857717
3	14.75385900732461	8.319070755903086	61.054105864825914	15.872964371946388
4	13.376810536191797	3.38462453595927	64.28001697990261	18.958547947946325
5	17.80131891663895	3.8150704747072455	59.661104937420404	18.722505671233396
6	16.510251481009817	8.095195606855771	61.775210964474695	13.619341947659722
7	61.23228668997348	1.3256761543224396	34.62494153019205	2.817095625512033
8	63.051677846905086	5.16048441390946	28.77823111594191	3.0096066232435397
9	58.79074973840675	2.6440520320455105	29.08295006881187	9.482248160735868
10	32.53381785539504	18.693845326065503	35.31333057545106	13.459006243088394
11	25.396040005515765	18.146324581112832	41.77975335880319	14.677882054568217
12	21.717241360663404	16.391284010501582	45.74812964209718	16.14334498673783
13	18.95368109688008	16.719526076858394	49.19467133884369	15.132121487417837
14	16.84822724949912	17.173224748478432	47.31255187928046	18.665996122741983
15	15.240544113949205	16.512144145313357	51.21252186703222	17.034789873705215
16	17.728316150645263	16.5518900956877	45.498027573415094	20.22176618025194
17	17.756165353968782	16.616511062622855	47.46666882971159	18.16065475369678
18	18.05169136593583	16.38830982373888	46.704465876614506	18.855532933710784
19	18.25636949133295	19.54338121774021	43.49748140457322	18.70276788635362
20	20.38669835527472	19.431443643216557	45.541288471781726	14.640569529726996
21	20.187157461558634	20.225010747629437	43.753802227395504	15.834029563416422
22	18.37074049138973	17.067505928094977	45.65728175552726	18.904471824988036
23	18.45509924320466	20.608410459403704	44.689319154573894	16.247171142817745
24	18.486193013905673	17.72993843433401	44.77773361561069	19.006134936149618
25	18.08386665909601	20.54487101492771	42.25481209899175	19.116450226984526
26	17.386825434163672	18.637606158188884	44.63443188977123	19.341136517876215
27	17.834034971028718	18.859859023547447	43.63997198856831	19.666134016855526
28	15.66855662716406	20.145789227495545	45.34742557097626	18.838228574364134
29	19.466863503754233	19.875949373933686	42.33646704465877	18.320720077653313
30	19.29354952967292	19.196482888962795	44.29726726312631	17.212700318237985
31	19.92732169074406	19.14673285584117	42.91643346338641	18.00951199002836
32	22.144442732033884	19.847018648151003	40.864785358348946	17.143753261466166
33	20.026280995757727	19.75698190342545	39.525049412057356	20.691687688759465
34	19.665863636240736	22.670062647188445	40.717968684517196	16.94610503205362
35	21.208385043625913	23.10159010839559	37.50368393587654	18.18634091210197
36	19.660185643330117	25.822159854427078	36.42135033486639	18.096304167376413
37	22.498641337410675	23.845407179686845	36.13772106995017	17.518230412952313
38	20.943952802359885	22.918001670952197	36.308331237883564	19.82971428880435
39	22.16634356183199	23.133495020940977	35.7954192116242	18.904742205602826
40	23.27679674678044	22.067113876203532	35.73809852128842	18.917990855727606
41	19.322750636070396	25.253549421520677	36.33401739628875	19.089682546120173
42	20.86635356591474	23.473904214963405	36.67767115768868	18.982071061433178
43	20.612736549240367	23.348177229085383	35.062958126154186	20.976128095520064
44	21.80781886661854	22.49837095679588	34.862335709978936	20.831474466606643
45	20.117399262942445	21.726163920951524	35.47339589940759	22.683040916698435
46	21.30491092310646	23.599090439611842	34.3959291494637	20.700069487818002
47	18.260965961784404	22.95936990501529	38.765550265108196	20.014113868092114
48	19.761578373876905	22.892856273776594	35.958458722343444	21.387106630003057
49	19.691549794645923	22.213930550035286	37.12461031393893	20.96990934137986
50	19.0856268368983	22.08982584784601	36.703357316093864	22.12118999916182
51	19.48092329572339	21.4363159018951	35.79190426363191	23.290856538749598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	85.0
1	460.0
2	835.0
3	15107.5
4	29380.0
5	19460.0
6	9540.0
7	8834.5
8	8129.0
9	7970.0
10	7811.0
11	7405.5
12	7000.0
13	6700.5
14	6401.0
15	5837.0
16	5273.0
17	4897.0
18	4521.0
19	4197.5
20	3874.0
21	3534.5
22	3195.0
23	3134.0
24	3073.0
25	3149.5
26	3389.0
27	3552.0
28	4008.5
29	4465.0
30	4926.5
31	5388.0
32	6086.5
33	6785.0
34	7423.0
35	8061.0
36	8637.0
37	9213.0
38	9559.5
39	9906.0
40	10614.5
41	11323.0
42	11837.0
43	12351.0
44	13271.5
45	14192.0
46	22647.5
47	31103.0
48	26778.5
49	22454.0
50	21739.0
51	21024.0
52	19503.5
53	17983.0
54	17395.0
55	16807.0
56	16369.0
57	15931.0
58	15406.5
59	14882.0
60	13949.0
61	13016.0
62	11983.0
63	10950.0
64	9612.5
65	8275.0
66	7203.0
67	6131.0
68	5213.0
69	4295.0
70	3737.5
71	3180.0
72	2751.5
73	2323.0
74	1997.5
75	1377.5
76	1083.0
77	860.0
78	637.0
79	431.0
80	225.0
81	185.5
82	146.0
83	120.0
84	94.0
85	62.0
86	30.0
87	24.5
88	19.0
89	12.0
90	5.0
91	3.0
92	1.0
93	1.5
94	2.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	369849.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.771938215856785
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.78070586459955	38.98305254327321
2	9.110188116699286	9.250838163917775
3	4.638857937340561	7.065714257603756
4	2.928405398821332	5.947232719197524
5	1.9825936570988523	5.033006133268623
6	1.3001508514430935	3.96067072204574
7	0.9246266611385034	3.2861561398440626
8	0.6883874977690393	2.796061400423833
9	0.45260512365372746	2.0681675436894547
>10	1.1225329973702958	8.349638409963712
>50	0.040384585852803856	1.4247534883843238
>100	0.026195407039656504	2.406176447516424
>500	0.0016372129399785315	0.5849192170321477
>1k	0.002182950586638042	3.34188094581939
>5k	0.0	0.0
>10k+	5.457376466595105E-4	5.50173186802005
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19856	5.3686774872988705	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCG	4767	1.2889043907108035	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTC	3224	0.8717071020876087	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGC	2960	0.8003266197826681	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCT	1110	0.30012248241850054	No Hit
GCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTCTTCTGC	811	0.21927867859585937	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTGCTGAT	674	0.18223653436943185	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTGCTGATCGTATGCCGTC	626	0.16925826485944265	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTGCTGATCGTA	409	0.11058567144969975	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06570248939432038	0.0
2	0.0	0.0	0.0	0.3936741751363394	0.0
3	0.0	0.0	0.0	0.5210234447031086	0.0
4	0.0	0.0	0.0	1.028527858666645	0.0
5	0.0	0.0	0.0	2.2628153651895775	0.0
6	0.0	0.0	0.0	2.6721716159838205	0.0
7	0.0	0.0	0.0	3.048271051158716	0.0
8	0.0	0.0	0.0	3.5287374036431083	0.0
9	0.0	0.0	0.0	3.8085813399522506	0.0
10	0.0	0.0	0.0	5.618238794751371	0.0
11	0.0	0.0	0.0	6.1146576035084585	0.0
12	0.0	0.0	0.0	7.341915214046813	0.0
13	0.0	0.0	0.0	7.520366419809165	0.0
14	0.0	0.0	0.0	7.656097488434469	0.0
15	0.0	0.0	0.0	7.934859902284446	0.0
16	0.0	0.0	0.0	8.11547415296513	0.0
17	0.0	0.0	0.0	8.27310605138854	0.0
18	0.0	0.0	0.0	8.429115666123202	0.0
19	0.0	0.0	0.0	8.793318354247274	0.0
20	0.0	0.0	0.0	8.96879537324692	0.0
21	0.0	0.0	0.0	9.144542772861357	0.0
22	0.0	0.0	0.0	9.397078267076564	0.0
23	0.0	0.0	0.0	9.593644974029942	0.0
24	2.703806147914419E-4	0.0	0.0	9.748573066305438	0.0
25	2.703806147914419E-4	0.0	0.0	9.877003858331372	0.0
26	2.703806147914419E-4	0.0	0.0	10.007327314660849	0.0
27	2.703806147914419E-4	0.0	0.0	10.16171464570676	0.0
28	2.703806147914419E-4	0.0	0.0	10.292038102036237	0.0
29	2.703806147914419E-4	0.0	0.0	10.44994038107444	0.0
30	2.703806147914419E-4	0.0	0.0	10.617846742859925	0.0
31	2.703806147914419E-4	0.0	0.0	10.763852274847302	0.0
32	2.703806147914419E-4	0.0	0.0	10.922836076344671	0.0
33	2.703806147914419E-4	0.0	0.0	11.056674480666434	0.0
34	2.703806147914419E-4	0.0	0.0	11.222147416918796	0.0
35	2.703806147914419E-4	0.0	0.0	11.41222498911718	0.0
36	2.703806147914419E-4	0.0	0.0	11.578238686599125	0.0
37	2.703806147914419E-4	0.0	0.0	11.746685809614194	0.0
38	2.703806147914419E-4	0.0	0.0	11.937844904271744	0.0
39	2.703806147914419E-4	0.0	0.0	12.262842403251057	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCAGA	20	7.0270745E-4	45.0	16
GCGGCAA	20	7.0270745E-4	45.0	5
ACATACG	50	2.1827873E-11	45.0	17
TATCGGG	20	7.0270745E-4	45.0	3
CGTTTTT	8520	0.0	44.154926	1
TGATACC	595	0.0	43.487396	4
GCGGGAT	130	0.0	43.26923	5
GATACCT	610	0.0	42.78688	5
TACGGCT	395	0.0	41.58228	7
GAATGAT	1630	0.0	41.27301	1
GCGATGC	60	3.6379788E-12	41.250004	9
ATGATAC	1545	0.0	40.92233	3
AATGATA	1530	0.0	40.88235	2
TTGGGAC	580	0.0	40.73276	5
CGACCAC	390	0.0	40.384617	12
ATACGGC	950	0.0	40.263157	6
ACTAATC	220	0.0	39.886364	10
GGATCCC	40	3.4497498E-7	39.375	8
CACCGGT	80	0.0	39.375	16
GTTTTTT	9720	0.0	39.25926	2
>>END_MODULE