FastQCFastQC Report
Sat 18 Jun 2016
SRR3549529_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549529_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621385
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161010.6142172935299469No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT43700.16670576813402074No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC38400.14648744842897932No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC37250.14210045453071565No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC35480.13534829870469237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT81300.041.7066461
TTGGGAC51350.036.8500485
ACACGCG18150.036.69421436
TTACGGG6950.036.5827333
TGGGCGA46500.036.3870966
TTTGGGC66700.036.364324
TTACACG18850.036.16710734
TTTGGGA126100.036.078514
ACGGGTA1000.035.9999965
TACCGGT1700.035.73529440
TATGGGC7850.035.54144
CGTTCAT4000.035.43749617
TTGGGAT58100.035.3184175
TCGTTCA3950.035.31645616
GGGCGAT70650.035.1592377
TTGGGAG71350.035.066575
CGTTTGG17950.034.9721452
CGTTGAT2450.034.8979625
CGAGATA21900.034.72602519
AGGCACG3050.034.6721310
Produced by FastQC (version 0.11.5)