##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549529_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2621385
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.17231730554649	33.0	31.0	34.0	30.0	34.0
2	32.36702277612789	34.0	31.0	34.0	30.0	34.0
3	32.43169889199793	34.0	31.0	34.0	30.0	34.0
4	35.92938084256986	37.0	35.0	37.0	35.0	37.0
5	35.12769623691293	37.0	35.0	37.0	35.0	37.0
6	34.822884849039724	37.0	35.0	37.0	32.0	37.0
7	35.61967471393939	37.0	35.0	37.0	33.0	37.0
8	35.908744804750164	37.0	35.0	37.0	35.0	37.0
9	37.76767624747986	39.0	38.0	39.0	35.0	39.0
10	37.25993625507127	39.0	37.0	39.0	34.0	39.0
11	37.184317831985766	39.0	37.0	39.0	34.0	39.0
12	36.97746267717256	39.0	37.0	39.0	33.0	39.0
13	36.90091650024701	39.0	37.0	39.0	33.0	39.0
14	37.903648643751296	40.0	37.0	41.0	33.0	41.0
15	38.030369060630164	40.0	37.0	41.0	33.0	41.0
16	38.066469061202376	40.0	37.0	41.0	33.0	41.0
17	38.034701884690726	40.0	37.0	41.0	33.0	41.0
18	37.97060714088163	40.0	37.0	41.0	33.0	41.0
19	37.93852982297526	40.0	37.0	41.0	33.0	41.0
20	37.81154809385115	40.0	36.0	41.0	33.0	41.0
21	37.68976399880216	40.0	36.0	41.0	33.0	41.0
22	37.76292379791599	40.0	36.0	41.0	33.0	41.0
23	37.694330287233655	39.0	36.0	41.0	33.0	41.0
24	37.62596490023404	39.0	35.0	41.0	33.0	41.0
25	37.50734249261364	39.0	35.0	41.0	33.0	41.0
26	37.461422110830725	39.0	35.0	41.0	33.0	41.0
27	37.429449317822446	39.0	35.0	41.0	33.0	41.0
28	37.33155793597659	39.0	35.0	41.0	32.0	41.0
29	37.2408310873832	39.0	35.0	41.0	32.0	41.0
30	37.13154267686738	39.0	35.0	41.0	32.0	41.0
31	37.01935579855687	39.0	35.0	41.0	31.0	41.0
32	36.958583725778546	39.0	35.0	41.0	31.0	41.0
33	36.8103960311057	39.0	35.0	41.0	31.0	41.0
34	36.61254451368265	39.0	35.0	41.0	31.0	41.0
35	36.43922315875005	39.0	35.0	41.0	30.0	41.0
36	36.29829231494038	39.0	35.0	41.0	30.0	41.0
37	36.27233351835003	39.0	35.0	41.0	30.0	41.0
38	36.153781302632005	39.0	35.0	40.0	30.0	41.0
39	36.10418767178419	39.0	35.0	40.0	30.0	41.0
40	35.970788342803516	38.0	35.0	40.0	29.0	41.0
41	35.80145686345195	38.0	35.0	40.0	29.0	41.0
42	35.723709413153735	38.0	35.0	40.0	29.0	41.0
43	35.6121306103453	38.0	35.0	40.0	28.0	41.0
44	35.549527062983884	38.0	34.0	40.0	28.0	41.0
45	35.540177425292356	38.0	34.0	40.0	28.0	41.0
46	35.339328637342476	38.0	34.0	40.0	28.0	41.0
47	35.24296087755137	37.0	34.0	40.0	27.0	41.0
48	35.13150491057208	37.0	34.0	40.0	27.0	41.0
49	35.17366888114489	37.0	34.0	40.0	27.0	41.0
50	34.97197740888881	37.0	34.0	40.0	27.0	41.0
51	33.897153603915484	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	14.0
9	22.0
10	49.0
11	44.0
12	56.0
13	46.0
14	70.0
15	108.0
16	176.0
17	376.0
18	756.0
19	1236.0
20	2330.0
21	3721.0
22	5599.0
23	8423.0
24	12641.0
25	19506.0
26	27128.0
27	32284.0
28	35938.0
29	40937.0
30	49533.0
31	61625.0
32	80197.0
33	112490.0
34	180352.0
35	206553.0
36	236153.0
37	344090.0
38	532283.0
39	626421.0
40	227.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.533590067845815	4.236310194801603	54.29927309418494	17.930826643167638
2	21.301449424636214	4.243749010542137	52.12618520362328	22.328616361198375
3	20.59369379164068	4.2146804074945114	52.34503897748709	22.846586823377717
4	18.654032124239667	4.770646051610123	51.381044753059925	25.194277071090283
5	17.519746241013813	7.652901042769376	50.514441793174214	24.31291092304259
6	21.689221537469695	7.359659111500218	53.0838087499547	17.86731060107539
7	82.49593249370085	2.137457870553162	10.822637651470501	4.543971984275488
8	85.13968760788667	2.3609656727264405	8.501574549331746	3.9977721700551423
9	79.45284649145394	4.84369903695947	10.619233725683179	5.084220745903406
10	40.076715171560075	25.882195862111057	17.68702422574326	16.354064740585606
11	31.451122212113063	22.708911510518295	25.431022150504408	20.408944126864235
12	26.560158084371427	20.288015686364268	32.188442369205596	20.96338386005871
13	24.827562528968468	20.171436091989538	34.39399401461441	20.60700736442758
14	21.68624601117348	21.73579996833735	35.04613019453457	21.5318238259546
15	20.25169900644125	20.91276176525005	36.38343852581746	22.45210070249124
16	23.57414115057498	20.83146886092657	32.79338975388964	22.801000234608804
17	23.972899822040638	20.91905614779973	31.02489714406697	24.083146886092656
18	25.01341847916273	20.224537792045044	31.804294294809804	22.957749433982418
19	24.987249106865264	22.60949841400634	29.53923212347671	22.864020355651686
20	25.619319558172492	24.303221388693384	28.691321572374907	21.386137480759217
21	25.811660629781585	23.903318284036875	29.091568006988673	21.19345307919287
22	24.342551742685643	21.895257659595977	29.831177030462904	23.931013567255476
23	22.921280162967285	23.492352325202134	29.277080627225683	24.309286884604894
24	23.14486426068662	23.145283886189937	29.99299988364929	23.71685196947415
25	24.576130556938413	23.655815532628743	27.30682444585591	24.46122946457693
26	23.248168430047475	22.938484808603086	28.051087497639603	25.76225926370983
27	22.621362371418165	21.776084016655318	30.42918152045579	25.173372091470732
28	21.41272647856	22.89926889792991	29.770712810212924	25.917291813297172
29	22.900756661078017	22.949509515008288	28.311713082969497	25.838020740944195
30	24.711288116777965	21.438857703084437	29.349866578163837	24.499987601973768
31	24.519366670672184	22.924103098171386	28.27203939901998	24.284490832136445
32	25.314366260583622	22.91952536540798	27.077975955458662	24.688132418549735
33	24.487780314604684	22.16294058293612	27.071185651859608	26.278093450599588
34	22.47262420438051	22.232751007578056	30.05243411402751	25.242190674013926
35	22.71730402058454	23.832668608388314	28.2095533467995	25.240474024227648
36	23.70910797154939	24.47564932278166	27.082820722633265	24.732421983035685
37	21.890794370151657	24.997167527852643	28.293173265277705	24.818864836717992
38	21.954195968924825	24.825655140317046	28.574017170312636	24.646131720445492
39	21.74968575771968	22.967171933920426	28.50786893188143	26.775273376478463
40	23.639030512496255	22.654512786179826	29.775366838522384	23.931089862801535
41	21.025984355598283	23.784030197777128	29.40369308590688	25.78629236071771
42	21.45190424146014	23.900228314421575	29.731496899539746	24.916370544578534
43	21.70650247865155	21.96861582712955	30.83251792468485	25.492363769534045
44	22.880156863642693	22.014736484720864	28.99959372621725	26.105512925419195
45	22.674349628154584	21.871758631410497	27.667473492066218	27.786418248368705
46	22.472776795472623	22.190178092878384	29.24778313753989	26.089261974109107
47	20.844744285940447	23.741266544212316	30.491171651626907	24.92281751822033
48	20.852297545000066	23.968779862553575	29.684269956530613	25.49465263591575
49	22.53129547929816	22.029537820655875	30.125143769419598	25.31402293062637
50	21.012823373903487	22.180603001848258	29.729856545299526	27.076717078948725
51	20.367897123085697	22.002185867394527	28.971822147452585	28.65809486206719
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	437.0
1	1727.5
2	3018.0
3	14753.5
4	26489.0
5	17398.0
6	8307.0
7	8235.5
8	8164.0
9	8038.5
10	7913.0
11	7885.0
12	7857.0
13	7840.0
14	7823.0
15	7526.0
16	7229.0
17	6885.5
18	6542.0
19	6915.0
20	7288.0
21	7003.5
22	6719.0
23	7806.0
24	8893.0
25	10732.5
26	15679.0
27	18786.0
28	20979.5
29	23173.0
30	27211.0
31	31249.0
32	36882.0
33	42515.0
34	48247.5
35	53980.0
36	60259.5
37	66539.0
38	72454.0
39	78369.0
40	85421.0
41	92473.0
42	101767.0
43	111061.0
44	126921.0
45	142781.0
46	166957.0
47	191133.0
48	218941.0
49	246749.0
50	246399.0
51	246049.0
52	226769.5
53	207490.0
54	195599.0
55	183708.0
56	174633.5
57	165559.0
58	155192.0
59	144825.0
60	132688.5
61	120552.0
62	109680.5
63	98809.0
64	87491.0
65	76173.0
66	64382.0
67	52591.0
68	45626.0
69	38661.0
70	32649.5
71	26638.0
72	21794.5
73	16951.0
74	14474.5
75	9443.0
76	6888.0
77	5014.5
78	3141.0
79	2484.5
80	1828.0
81	1323.5
82	819.0
83	579.5
84	340.0
85	251.0
86	162.0
87	126.0
88	90.0
89	61.5
90	33.0
91	20.5
92	8.0
93	9.0
94	10.0
95	6.0
96	2.0
97	1.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2621385.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.157126902163615
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71569995017751	20.75622318178964
2	10.133716683572468	5.706726932998452
3	3.6615059763567297	3.092924652879208
4	1.8787468304311838	2.11600451685954
5	1.150942129655547	1.6203611800878845
6	0.785481352727285	1.3270138876815185
7	0.5850254882942301	1.153084584043061
8	0.48415116624203725	1.0905844662166044
9	0.39034749684885406	0.9891957604243585
>10	6.484925631601207	43.91176947743956
>50	0.6441708306432982	11.289241819403232
>100	0.077648420646046	3.9941397233943383
>500	0.004721699187703603	0.8973308061702231
>1k	0.002777470110413884	1.4258050019777255
>5k	0.0	0.0
>10k+	1.388735055206942E-4	0.6295940086345984
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16101	0.6142172935299469	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	4370	0.16670576813402074	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	3840	0.14648744842897932	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	3725	0.14210045453071565	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	3548	0.13534829870469237	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1444331908514011E-4	0.0	0.0	0.17860787331887532	0.0
2	1.1444331908514011E-4	0.0	0.0	0.6520217366010715	0.0
3	1.1444331908514011E-4	0.0	0.0	1.01141190630144	0.0
4	1.1444331908514011E-4	0.0	0.0	1.4776539882543007	0.0
5	1.1444331908514011E-4	0.0	0.0	2.290773770354221	0.0
6	1.1444331908514011E-4	0.0	0.0	3.1892301207186278	0.0
7	1.1444331908514011E-4	0.0	0.0	3.96439286865531	0.0
8	1.1444331908514011E-4	0.0	0.0	5.1154637720136495	0.0
9	1.1444331908514011E-4	0.0	0.0	5.916147380106318	0.0
10	1.1444331908514011E-4	0.0	0.0	6.990960885180925	0.0
11	1.1444331908514011E-4	0.0	0.0	8.494555359094525	0.0
12	1.1444331908514011E-4	0.0	0.0	9.733366140418138	0.0
13	1.1444331908514011E-4	0.0	0.0	10.178283617248134	0.0
14	1.1444331908514011E-4	0.0	0.0	10.38489195596984	0.0
15	1.1444331908514011E-4	0.0	0.0	10.600960942402585	0.0
16	1.1444331908514011E-4	0.0	0.0	11.0480528422952	0.0
17	1.1444331908514011E-4	0.0	0.0	11.644455125820892	0.0
18	1.1444331908514011E-4	0.0	0.0	12.300749413001142	0.0
19	1.1444331908514011E-4	0.0	0.0	12.738952881778143	0.0
20	1.1444331908514011E-4	0.0	0.0	13.248340095026103	0.0
21	1.1444331908514011E-4	0.0	0.0	13.958460889949396	0.0
22	1.1444331908514011E-4	0.0	0.0	14.699061755522367	0.0
23	1.1444331908514011E-4	0.0	0.0	15.40922069821869	0.0
24	1.1444331908514011E-4	0.0	0.0	15.980788781502907	0.0
25	1.5259109211352014E-4	0.0	0.0	16.487391207319796	0.0
26	1.5259109211352014E-4	0.0	0.0	16.932384979695847	0.0
27	1.5259109211352014E-4	0.0	0.0	17.41388617086006	0.0
28	1.5259109211352014E-4	0.0	0.0	17.888978536155506	0.0
29	1.5259109211352014E-4	0.0	0.0	18.412098947693682	0.0
30	1.5259109211352014E-4	0.0	0.0	19.077090927124402	0.0
31	1.5259109211352014E-4	0.0	0.0	19.592696227375985	0.0
32	1.5259109211352014E-4	0.0	0.0	20.11280296484492	0.0
33	1.5259109211352014E-4	0.0	0.0	20.642522941117004	0.0
34	1.5259109211352014E-4	0.0	0.0	21.193796409150124	0.0
35	1.5259109211352014E-4	0.0	0.0	21.826286485960665	0.0
36	1.5259109211352014E-4	0.0	0.0	22.389309468086527	0.0
37	1.9073886514190018E-4	0.0	0.0	22.926239373460977	0.0
38	1.9073886514190018E-4	0.0	0.0	23.544385887612847	0.0
39	1.9073886514190018E-4	0.0	0.0	24.46271722772504	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	8130	0.0	41.706646	1
TTGGGAC	5135	0.0	36.850048	5
ACACGCG	1815	0.0	36.694214	36
TTACGGG	695	0.0	36.582733	3
TGGGCGA	4650	0.0	36.387096	6
TTTGGGC	6670	0.0	36.36432	4
TTACACG	1885	0.0	36.167107	34
TTTGGGA	12610	0.0	36.07851	4
ACGGGTA	100	0.0	35.999996	5
TACCGGT	170	0.0	35.735294	40
TATGGGC	785	0.0	35.5414	4
CGTTCAT	400	0.0	35.437496	17
TTGGGAT	5810	0.0	35.318417	5
TCGTTCA	395	0.0	35.316456	16
GGGCGAT	7065	0.0	35.159237	7
TTGGGAG	7135	0.0	35.06657	5
CGTTTGG	1795	0.0	34.972145	2
CGTTGAT	245	0.0	34.89796	25
CGAGATA	2190	0.0	34.726025	19
AGGCACG	305	0.0	34.67213	10
>>END_MODULE