##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549526_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1123206
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09744428003412	33.0	31.0	34.0	30.0	34.0
2	32.272101466694444	34.0	31.0	34.0	30.0	34.0
3	32.35645910011164	34.0	31.0	34.0	30.0	34.0
4	35.87558916173881	37.0	35.0	37.0	35.0	37.0
5	35.08024084629177	37.0	35.0	37.0	33.0	37.0
6	34.70959467809111	37.0	35.0	37.0	32.0	37.0
7	35.55779260438423	37.0	35.0	37.0	33.0	37.0
8	35.804566571047516	37.0	35.0	37.0	35.0	37.0
9	37.69304116965187	39.0	37.0	39.0	35.0	39.0
10	37.171203679467524	39.0	37.0	39.0	34.0	39.0
11	37.082726587999	39.0	37.0	39.0	33.0	39.0
12	36.94134557685767	39.0	37.0	39.0	33.0	39.0
13	36.86680448644327	39.0	37.0	39.0	33.0	39.0
14	37.98956024095313	40.0	37.0	41.0	33.0	41.0
15	38.06933278490322	40.0	37.0	41.0	33.0	41.0
16	38.082051734054126	40.0	37.0	41.0	33.0	41.0
17	38.00578166427174	40.0	37.0	41.0	33.0	41.0
18	37.97146026641595	40.0	37.0	41.0	33.0	41.0
19	37.97909466295586	40.0	37.0	41.0	33.0	41.0
20	37.878739073687285	40.0	36.0	41.0	33.0	41.0
21	37.78720377205962	40.0	36.0	41.0	33.0	41.0
22	37.83052173866593	40.0	36.0	41.0	33.0	41.0
23	37.757429180399676	39.0	36.0	41.0	33.0	41.0
24	37.685959654773924	39.0	36.0	41.0	33.0	41.0
25	37.563940185504705	39.0	36.0	41.0	33.0	41.0
26	37.526905126931304	39.0	36.0	41.0	33.0	41.0
27	37.48402964371629	39.0	36.0	41.0	33.0	41.0
28	37.39879416598558	39.0	36.0	41.0	32.0	41.0
29	37.312142207217555	39.0	35.0	41.0	32.0	41.0
30	37.269279188323424	39.0	35.0	41.0	32.0	41.0
31	37.20743478934408	39.0	35.0	41.0	32.0	41.0
32	37.154866516026445	39.0	35.0	41.0	32.0	41.0
33	37.077393639278995	39.0	35.0	41.0	32.0	41.0
34	36.95526288143048	39.0	35.0	41.0	31.0	41.0
35	36.807809965402605	39.0	35.0	41.0	31.0	41.0
36	36.67971948155547	39.0	35.0	41.0	31.0	41.0
37	36.66780626171869	39.0	35.0	41.0	31.0	41.0
38	36.54016716434919	39.0	35.0	40.0	30.0	41.0
39	36.50894760177563	39.0	35.0	40.0	30.0	41.0
40	36.44077845025757	39.0	35.0	40.0	30.0	41.0
41	36.35942560848144	39.0	35.0	40.0	30.0	41.0
42	36.2771975933177	38.0	35.0	40.0	30.0	41.0
43	36.12440549640939	38.0	35.0	40.0	30.0	41.0
44	36.02903118395023	38.0	35.0	40.0	30.0	41.0
45	36.03722736523844	38.0	35.0	40.0	30.0	41.0
46	35.84142178727678	38.0	35.0	40.0	29.0	41.0
47	35.7394146754914	38.0	35.0	40.0	29.0	41.0
48	35.675924095847066	38.0	34.0	40.0	29.0	41.0
49	35.7334656331964	38.0	35.0	40.0	29.0	41.0
50	35.53954038707058	38.0	34.0	40.0	28.0	41.0
51	34.43011967528663	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	12.0
10	22.0
11	20.0
12	25.0
13	24.0
14	31.0
15	42.0
16	81.0
17	110.0
18	250.0
19	472.0
20	795.0
21	1386.0
22	2095.0
23	3107.0
24	4362.0
25	6326.0
26	8516.0
27	10523.0
28	12421.0
29	15167.0
30	19450.0
31	25015.0
32	33139.0
33	46197.0
34	75372.0
35	96383.0
36	104904.0
37	151158.0
38	235989.0
39	269720.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.00573714884002	4.634501596323382	53.8311761155122	15.528585139324399
2	19.9608086139141	6.761537954747393	52.083233173611966	21.19442025772654
3	19.92118987968369	6.5366459937001755	51.492513394693404	22.04965073192273
4	18.562489872739285	4.985105136546635	52.082966081021645	24.369438909692434
5	18.400542732143524	8.402999983974444	48.8891619168701	24.30729536701193
6	20.95519432766563	9.152550823268395	52.629348489947525	17.262906359118453
7	81.48353908365874	2.2386810611766674	11.3974640448858	4.880315810278791
8	83.09820282299061	4.07129235420751	7.845221624528359	4.985283198273514
9	77.53599962963162	5.45501003377831	9.613997788473352	7.39499254811673
10	38.88663344034843	27.985338397408842	17.482634530086198	15.645393632156523
11	29.029314302095965	24.758948937238586	25.260281729264268	20.951455031401185
12	24.817620276244963	21.859391776753327	31.754370970240544	21.568616976761167
13	23.087750599622865	24.36160419370979	33.425124153539066	19.125521053128278
14	20.728076595032434	26.107232333160614	31.38685156596386	21.777839505843097
15	18.508002984314544	23.59905484835373	35.744645238718455	22.148296928613274
16	20.643052120448075	22.98411867457973	32.033215634531864	24.33961357044033
17	20.319157839256558	22.94156192185583	29.939654880760962	26.79962535812665
18	22.104849867254984	22.468986098720983	31.648513273611428	23.777650760412605
19	22.278994236141898	24.58836580288923	29.381075243543926	23.75156471742494
20	24.71069420925458	24.654871857878252	29.63552545125293	20.998908481614237
21	24.504053575212385	25.958906914671037	28.351433307870504	21.18560620224607
22	22.252730131427363	23.73865524222627	28.252430987726207	25.75618363862016
23	21.557755211421593	26.518465891385905	26.833635147960393	25.09014374923211
24	22.349773772576	24.127542053728344	29.018185444166072	24.50449872952958
25	21.660496827830336	26.68326201961172	26.65076575445644	25.005475398101506
26	20.853343019891277	25.915905007629945	27.67818191854388	25.552570053934897
27	21.291463898875186	25.343525586579847	28.37520454840875	24.98980596613622
28	19.582249382570964	25.438343456142505	30.72918057773908	24.25022658354745
29	21.046539993554163	25.437898301825314	28.209518111548547	25.306043593071976
30	21.7712512219486	25.174455976908956	28.759194662421677	24.295098138720768
31	23.686750248841264	26.635986631125547	24.873887781938485	24.803375338094703
32	22.16779468770644	28.218955383073098	25.857411730350442	23.75583819887002
33	22.612503850584844	25.041176774340595	26.11408770964543	26.23223166542914
34	21.460444477682632	25.143384205568704	27.256798841886525	26.13937247486214
35	21.020097827112746	25.66287929373597	27.342624594241837	25.974398284909444
36	23.85510761160464	26.283246350179752	26.307284683308314	23.55436135490729
37	22.11704709554614	26.441187101920754	27.03920741164132	24.402558390891787
38	22.195394255372566	27.119602281326845	27.610340400603274	23.074663062697315
39	22.013682263093322	25.50155536918428	28.441087387353697	24.043674980368696
40	21.651504710622984	23.63546847150033	30.55539233230592	24.157634485570767
41	20.393053455911026	24.126028529049883	28.133485754171538	27.347432260867556
42	22.569769036134065	24.416981390768925	28.119151785157843	24.894097787939167
43	24.011089684349976	23.57065400291665	26.79321513595903	25.62504117677434
44	21.849331289184708	24.1201524920629	27.80416059031024	26.226355628442157
45	22.073065849007218	24.03610735697637	26.681214309752622	27.209612484263797
46	22.946992804525618	24.373356267683754	28.216106395443045	24.463544532347584
47	19.76048917117608	24.96576763300766	30.659024257349053	24.61471893846721
48	21.294847071685872	24.207936923413868	29.063502153656586	25.433713851243674
49	21.462047033224536	23.023915470537016	30.96190725476894	24.55213024146951
50	21.686849963408314	22.593896400126066	29.2139643128687	26.50528932359692
51	20.54449495462097	22.176430681460037	27.885979953810786	29.393094410108205
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	374.0
1	850.5
2	1327.0
3	3294.5
4	5262.0
5	3605.0
6	1948.0
7	1982.5
8	2017.0
9	2082.0
10	2147.0
11	2425.5
12	2704.0
13	2649.0
14	2594.0
15	2517.0
16	2440.0
17	2437.0
18	2434.0
19	2561.5
20	2689.0
21	2865.0
22	3041.0
23	3638.0
24	4235.0
25	5445.5
26	7833.5
27	9011.0
28	10157.5
29	11304.0
30	13023.0
31	14742.0
32	17748.0
33	20754.0
34	22819.0
35	24884.0
36	27086.5
37	29289.0
38	33105.5
39	36922.0
40	43579.0
41	50236.0
42	56532.0
43	62828.0
44	72364.5
45	81901.0
46	100513.0
47	119125.0
48	122920.0
49	126715.0
50	118944.5
51	111174.0
52	96263.5
53	81353.0
54	72415.0
55	63477.0
56	57173.5
57	50870.0
58	47161.5
59	43453.0
60	41445.0
61	39437.0
62	35473.5
63	31510.0
64	27453.5
65	23397.0
66	20426.5
67	17456.0
68	14659.5
69	11863.0
70	9959.5
71	8056.0
72	6778.0
73	5500.0
74	4651.5
75	3032.5
76	2262.0
77	1696.5
78	1131.0
79	800.0
80	469.0
81	332.0
82	195.0
83	149.5
84	104.0
85	81.0
86	58.0
87	49.5
88	41.0
89	26.0
90	11.0
91	7.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1123206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.75776497283082
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.18195774821702	25.71732624834438
2	10.450310478111623	7.055582500264068
3	3.7346206738830268	3.782173409148548
4	1.8291108558759201	2.4698677752765095
5	1.0431579736307544	1.7607340851680724
6	0.6793335675321605	1.3759669746543217
7	0.4872741305724962	1.1514499904044762
8	0.3812995904664415	1.0297457567362218
9	0.30941835257674793	0.9400744822109721
>10	4.381120952591351	37.29454162097363
>50	0.4454234724154007	9.19744487242538
>100	0.06978813174492579	4.111723313071206
>500	0.003848610204751702	0.8882230064835295
>1k	0.003335462177451475	3.225145964838684
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGC	4865	0.43313515063131786	No Hit
GAATCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTC	4449	0.39609831144064406	No Hit
GCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	4267	0.3798946942947242	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCG	4248	0.37820310788938094	No Hit
TCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	3839	0.3417894847427809	No Hit
CTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGCT	3672	0.3269213305484479	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTGC	3462	0.3082248492262328	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2873	0.2557856706605912	No Hit
TCCTGTCTCTTATACACATCTGACGCACGATCTGTCGTATGCCGTCTTCTG	1386	0.12339677672662006	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7806172687823962E-4	0.0	0.0	0.3503364476329364	0.0
2	1.7806172687823962E-4	0.0	0.0	1.4929585490106	0.0
3	1.7806172687823962E-4	0.0	0.0	2.1515198458697693	0.0
4	1.7806172687823962E-4	0.0	0.0	2.970336696919354	0.0
5	1.7806172687823962E-4	0.0	0.0	4.517960196081574	0.0
6	1.7806172687823962E-4	0.0	0.0	5.320751491712117	0.0
7	1.7806172687823962E-4	0.0	0.0	6.165565354885925	0.0
8	1.7806172687823962E-4	0.0	0.0	7.418941850381853	0.0
9	1.7806172687823962E-4	0.0	0.0	8.101185356915828	0.0
10	1.7806172687823962E-4	0.0	0.0	9.619962856323774	0.0
11	1.7806172687823962E-4	0.0	0.0	11.550597130001087	0.0
12	1.7806172687823962E-4	0.0	0.0	13.685646266134619	0.0
13	2.670925903173594E-4	0.0	0.0	14.254820576100911	0.0
14	2.670925903173594E-4	0.0	0.0	14.458968345966813	0.0
15	2.670925903173594E-4	0.0	0.0	14.805565497335307	0.0
16	8.012777709520782E-4	0.0	0.0	15.521106546795512	0.0
17	8.012777709520782E-4	0.0	0.0	16.587251136478972	0.0
18	8.012777709520782E-4	0.0	0.0	17.753199324077684	8.903086343911981E-5
19	8.012777709520782E-4	0.0	0.0	18.598369310705248	8.903086343911981E-5
20	8.012777709520782E-4	0.0	0.0	19.357535483250622	8.903086343911981E-5
21	8.012777709520782E-4	0.0	0.0	20.370884770914685	8.903086343911981E-5
22	8.012777709520782E-4	0.0	0.0	21.41931221877376	8.903086343911981E-5
23	8.012777709520782E-4	0.0	0.0	22.43702401874634	8.903086343911981E-5
24	8.012777709520782E-4	0.0	0.0	23.24818421554016	8.903086343911981E-5
25	8.012777709520782E-4	0.0	0.0	23.93292058625043	8.903086343911981E-5
26	8.012777709520782E-4	0.0	0.0	24.544206494623428	8.903086343911981E-5
27	8.012777709520782E-4	0.0	0.0	25.144096452476216	8.903086343911981E-5
28	8.012777709520782E-4	0.0	0.0	25.765531879281273	8.903086343911981E-5
29	8.012777709520782E-4	0.0	0.0	26.40886889849235	8.903086343911981E-5
30	8.012777709520782E-4	0.0	0.0	27.153879163750904	8.903086343911981E-5
31	8.012777709520782E-4	0.0	0.0	27.794456226195372	8.903086343911981E-5
32	8.012777709520782E-4	0.0	0.0	28.403071208665196	8.903086343911981E-5
33	8.012777709520782E-4	0.0	0.0	29.05922867221151	8.903086343911981E-5
34	8.012777709520782E-4	0.0	0.0	29.67523321634678	8.903086343911981E-5
35	8.012777709520782E-4	0.0	0.0	30.360058617920487	8.903086343911981E-5
36	8.012777709520782E-4	0.0	0.0	30.966625890531212	8.903086343911981E-5
37	8.012777709520782E-4	0.0	0.0	31.558948224991674	8.903086343911981E-5
38	8.012777709520782E-4	0.0	0.0	32.14824351009521	8.903086343911981E-5
39	8.012777709520782E-4	0.0	0.0	32.75009214694366	8.903086343911981E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTAAAC	30	2.1654578E-6	45.000004	45
CATTTCG	30	2.1654578E-6	45.000004	12
TAACGCC	50	2.1827873E-11	45.0	12
CTAAACG	20	7.03322E-4	45.0	45
TCGAAAC	25	3.8907492E-5	45.0	12
TACGTAA	25	3.8907492E-5	45.0	35
TACGGGA	160	0.0	42.1875	4
GCCGATT	255	0.0	40.588234	9
TCAAGCG	295	0.0	39.66102	17
TCCGATA	40	3.4581353E-7	39.375	14
TACGAAT	115	0.0	39.130436	12
TAGCGGG	175	0.0	38.571426	3
AACTACC	35	6.248163E-6	38.571426	17
TTTGGGA	5835	0.0	38.213367	4
TCTACGG	190	0.0	37.894737	2
ACGGGAT	125	0.0	37.800003	5
CGTTTTT	1685	0.0	37.789314	1
TTTTGCG	870	0.0	37.75862	1
GGCCGAT	485	0.0	37.57732	8
TTTAGCG	60	1.5643309E-10	37.500004	1
>>END_MODULE