FastQCFastQC Report
Sat 18 Jun 2016
SRR3549525_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549525_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174491
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC12760.7312698076118539TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGCT11590.6642176387320836Illumina Single End Adapter 1 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC10110.5793995105764768TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTGC7860.4504530319615338TruSeq Adapter, Index 27 (95% over 22bp)
CGGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT6860.3931434859104481No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4310.2470041434801795No Hit
TCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG4260.2441386661776252No Hit
TTCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCT3240.18568292920551777No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCC2670.1530164879563989No Hit
GCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG2410.1381160059831166TruSeq Adapter, Index 27 (95% over 21bp)
TTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG2390.1369698150620949No Hit
CGGTCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTC2110.12092314216779088No Hit
TGCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTCTG1900.10888813749706289No Hit
TTTCCTGTCTCTTATACACATCTGACGCAACGTTGCTCGTATGCCGTCTTC1880.10774194657604116No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC207.0168247E-445.0000046
ACACGAC453.8016879E-1045.00000426
CACGGGA207.0168247E-445.0000044
TTAGCGG207.0168247E-445.0000042
GACACGA453.8016879E-1045.00000425
TAGCGGG207.0168247E-445.0000043
AGTGACG207.0168247E-445.00000411
GCATAGA207.0168247E-445.00000440
TCTTATG351.2040255E-745.01
TATTCAC253.8771686E-545.022
ATGCGAA253.8771686E-545.026
TAATGCG253.8771686E-545.024
TACGGGA302.1548913E-644.9999964
AATGCGA302.1548913E-644.99999625
CCCCGAT302.1548913E-644.99999640
GCGATTC302.1548913E-644.9999969
CCCGATC302.1548913E-644.99999641
TTGTAGG650.041.5384642
ATGGGAC603.6379788E-1241.2499965
CGACCAA501.0695658E-940.529
Produced by FastQC (version 0.11.5)