FastQCFastQC Report
Sat 18 Jun 2016
SRR3549523_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549523_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455635
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC217801.4962542120792643No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG201991.3876418195495437No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC193511.3293854572059616No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC59830.4110233678085509TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCAGCTTT49960.34321790833553745No Hit
GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT44930.3086625424642853No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT44730.30728857165429524No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTC44470.30550240960130803No Hit
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT39890.2740384780525338TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC38340.2633902042751102TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36310.24944440055371023No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTA35940.2469025545552285No Hit
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC29260.20101192950155775TruSeq Adapter, Index 13 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAT253.8913193E-545.00000412
TAGTCCA253.8913193E-545.00000432
ATAGGCG207.033908E-445.035
TAAGCGT207.033908E-445.028
GCGATAC302.1659034E-644.9999969
ATGCGAA800.042.187526
TCGTTCA3950.042.151916
CGATGAA12050.041.0788419
CCGATGA11800.040.80508418
GCGATAT850.039.7058839
CGGGTAT1650.039.5454526
TCCGATA800.039.37514
TATGCGT403.459063E-739.37511
CTCGAAT403.459063E-739.37543
TAATACG1150.039.1304324
TTTGGGC43000.038.6686064
AACACGT3550.038.66197241
GACACGA3850.038.5714325
TACGGCT31000.038.395167
GGCGATA1650.038.1818168
Produced by FastQC (version 0.11.5)