##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549523_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1455635
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.038142803656136	33.0	31.0	34.0	30.0	34.0
2	32.200348301600336	34.0	31.0	34.0	30.0	34.0
3	32.29016065153696	34.0	31.0	34.0	30.0	34.0
4	35.8444369639367	37.0	35.0	37.0	35.0	37.0
5	35.03668570761214	37.0	35.0	37.0	33.0	37.0
6	34.519709267776605	37.0	35.0	37.0	30.0	37.0
7	35.47907751599817	37.0	35.0	37.0	33.0	37.0
8	35.6430471924624	37.0	35.0	37.0	33.0	37.0
9	37.601254435349524	39.0	37.0	39.0	35.0	39.0
10	37.11623312162733	39.0	37.0	39.0	33.0	39.0
11	37.05887671016429	39.0	37.0	39.0	33.0	39.0
12	36.94058469327819	39.0	37.0	39.0	33.0	39.0
13	36.8771828102512	39.0	37.0	39.0	33.0	39.0
14	38.00112116018095	40.0	37.0	41.0	33.0	41.0
15	38.00217980469005	40.0	37.0	41.0	33.0	41.0
16	38.090771381562	40.0	37.0	41.0	33.0	41.0
17	37.92547513628073	40.0	37.0	41.0	33.0	41.0
18	37.90728513672727	40.0	37.0	41.0	33.0	41.0
19	37.9180996609727	40.0	37.0	41.0	33.0	41.0
20	37.72927347858495	40.0	36.0	41.0	33.0	41.0
21	37.72001497628183	39.0	36.0	41.0	33.0	41.0
22	37.78560628179454	39.0	36.0	41.0	33.0	41.0
23	37.640716250983246	39.0	36.0	41.0	33.0	41.0
24	37.597976140996884	39.0	36.0	41.0	33.0	41.0
25	37.52697139049281	39.0	36.0	41.0	33.0	41.0
26	37.4954621179073	39.0	36.0	41.0	33.0	41.0
27	37.422041239733865	39.0	36.0	41.0	32.0	41.0
28	37.33498438825667	39.0	36.0	41.0	32.0	41.0
29	37.301444386814005	39.0	36.0	41.0	32.0	41.0
30	37.22213741769056	39.0	35.0	41.0	32.0	41.0
31	37.19703359702123	39.0	35.0	41.0	32.0	41.0
32	37.08042194643575	39.0	35.0	41.0	31.0	41.0
33	37.033548932252934	39.0	35.0	41.0	31.0	41.0
34	36.86773195203468	39.0	35.0	41.0	31.0	41.0
35	36.75891208991265	39.0	35.0	41.0	31.0	41.0
36	36.6551642410357	39.0	35.0	41.0	31.0	41.0
37	36.62693257581743	39.0	35.0	41.0	31.0	41.0
38	36.56407409824578	39.0	35.0	41.0	30.0	41.0
39	36.49129898635304	39.0	35.0	41.0	30.0	41.0
40	36.400533100674274	39.0	35.0	40.0	30.0	41.0
41	36.296366190700276	39.0	35.0	40.0	30.0	41.0
42	36.26443167414908	39.0	35.0	40.0	30.0	41.0
43	36.11535790222137	39.0	35.0	40.0	30.0	41.0
44	36.11455000738509	38.0	35.0	40.0	30.0	41.0
45	36.13207637903733	38.0	35.0	40.0	30.0	41.0
46	35.911045694834215	38.0	35.0	40.0	29.0	41.0
47	35.75331590680356	38.0	35.0	40.0	29.0	41.0
48	35.70519189219825	38.0	35.0	40.0	29.0	41.0
49	35.83484527371216	38.0	35.0	40.0	29.0	41.0
50	35.63107372383874	38.0	35.0	40.0	29.0	41.0
51	34.595508489422144	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	9.0
9	22.0
10	29.0
11	29.0
12	35.0
13	23.0
14	31.0
15	63.0
16	90.0
17	141.0
18	255.0
19	667.0
20	1245.0
21	2334.0
22	4033.0
23	5780.0
24	7298.0
25	9313.0
26	11496.0
27	13487.0
28	16183.0
29	19254.0
30	24569.0
31	32622.0
32	43467.0
33	59712.0
34	94836.0
35	121433.0
36	132147.0
37	189415.0
38	301423.0
39	364076.0
40	116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.06156419706863	4.344427002648329	50.52743304468497	14.066575755598073
2	19.342211474717217	12.166442823922207	48.16818776685089	20.32315793450968
3	19.607525238126318	12.377965630120189	47.57504456817815	20.439464563575346
4	17.72126941163135	4.434422090702683	54.14715914360399	23.69714935406197
5	21.795436355954617	7.655490559103072	46.42688586080988	24.122187224132425
6	20.15182377450391	13.60011266560642	50.29296492596015	15.95509863392952
7	78.08873790476322	1.8694933826130866	15.465896327032533	4.575872385591168
8	78.73924438475305	7.957351946057906	8.34446822177263	4.95893544741642
9	73.46443304811989	4.767403916503794	9.579667979953765	12.188495055422548
10	38.41388809694738	24.435727362972173	19.033136741009937	18.11724779907051
11	31.146750387288023	23.47738272300405	24.424186008168256	20.951680881539673
12	24.12974406358737	20.90063786594854	31.27926987191157	23.69034819855252
13	23.984996238754906	21.49975783764474	35.81371703758154	18.701528886018817
14	21.716776527082683	27.294479728778164	29.465147512941087	21.52359623119807
15	17.958828964678645	23.436507091406845	36.834989540647214	21.769674403267302
16	20.12654271160009	22.142982272341623	31.06348775620262	26.666987259855663
17	19.797201908445455	22.944144651646877	31.9161740408825	25.34247939902517
18	20.312784454894256	22.814716601345804	31.404301215620674	25.468197728139263
19	21.750713606089437	25.094271572200448	29.31847612897464	23.836538692735473
20	25.092897601390458	24.17996269669251	30.809646649056944	19.91749305286009
21	24.293727479759692	27.553335829380305	28.125800767362698	20.027135923497305
22	21.74480553160648	22.527968893300862	29.55383732872595	26.17338824636671
23	22.156584583360527	26.928591302077788	27.454822122304012	23.460001992257677
24	22.832646920416174	24.195969456628895	27.647933719648126	25.323449903306805
25	21.140395772291818	28.76490328962961	26.17139598869222	23.923304949386353
26	19.8326503553432	24.325672301091963	30.321337423186446	25.520339920378394
27	22.633558550048605	25.340074950107685	27.844961133800712	24.181405366043
28	18.387851350098067	24.837064236570296	32.32506775393556	24.45001665939607
29	21.87773720747303	23.05488669893208	30.491297612382223	24.576078481212665
30	23.50101502093588	25.840543817646594	28.974433838153107	21.684007323264417
31	23.13320303510152	24.455237748474033	25.25035465621533	27.161204560209118
32	23.66266268673122	28.156165522263482	26.195715272029048	21.985456518976253
33	24.335496192383392	22.941602805648394	25.543147835824225	27.179753166143982
34	21.49577332229577	23.32823819157962	28.732271482892347	26.443717003232265
35	22.008264434422088	22.89626176891872	29.876651770533137	25.218822026126052
36	21.97487694373933	23.953601005746634	30.194039027640855	23.87748302287318
37	22.075795099733106	25.928203155323963	28.59473700481233	23.401264740130596
38	22.014653398688544	25.604564331030787	26.08641589409433	26.29436637618634
39	21.939153702679587	25.874480896653353	27.344835758964305	24.84152964170276
40	22.74842250976378	22.123471886839763	30.834034630934266	24.29407097246219
41	19.076348122984125	23.34788597416248	30.16772748662955	27.408038416223846
42	20.217843071923937	22.785794515795512	32.98072662446286	24.015635787817686
43	21.661748996142578	22.261624651784274	30.518845727122528	25.55778062495062
44	22.243763031254403	22.65739694360193	28.861149944869418	26.237690080274245
45	21.854104909541196	21.865440168723616	27.841869699478234	28.43858522225695
46	25.425604633029568	24.045519652934974	27.748233588777403	22.780642125258048
47	19.574206445984053	23.14323302201445	33.612753197058325	23.66980733494317
48	20.62543151270751	23.89664991567254	29.374808932184237	26.10310963943571
49	21.108382252419048	21.373696015828145	33.19094415839135	24.326977573361454
50	22.432684017628045	20.584212388407806	30.022017882230088	26.961085711734057
51	20.54189408746011	20.807963534814704	28.43631817042047	30.213824207304718
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	295.0
1	949.5
2	1604.0
3	4041.0
4	6478.0
5	4436.0
6	2394.0
7	2468.0
8	2542.0
9	2568.5
10	2595.0
11	2798.0
12	3001.0
13	3002.0
14	3003.0
15	3068.0
16	3133.0
17	3252.5
18	3372.0
19	3614.5
20	3857.0
21	3851.0
22	3845.0
23	4254.0
24	4663.0
25	6128.0
26	8742.5
27	9892.0
28	12353.0
29	14814.0
30	16508.5
31	18203.0
32	21973.5
33	25744.0
34	29889.0
35	34034.0
36	36560.0
37	39086.0
38	43846.5
39	48607.0
40	54374.0
41	60141.0
42	67287.0
43	74433.0
44	84352.5
45	94272.0
46	147707.0
47	201142.0
48	180779.5
49	160417.0
50	152578.0
51	144739.0
52	125703.5
53	106668.0
54	94135.0
55	81602.0
56	75038.5
57	68475.0
58	61396.0
59	54317.0
60	51547.5
61	48778.0
62	43848.0
63	38918.0
64	33549.5
65	28181.0
66	24138.0
67	20095.0
68	16216.0
69	12337.0
70	10174.0
71	8011.0
72	6949.0
73	5887.0
74	4843.5
75	3034.0
76	2268.0
77	1752.5
78	1237.0
79	944.5
80	652.0
81	459.5
82	267.0
83	211.0
84	155.0
85	110.5
86	66.0
87	38.5
88	11.0
89	8.0
90	5.0
91	4.5
92	4.0
93	3.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1455635.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.88986650256691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.31421393119568	23.264460140803997
2	10.517140517338943	6.497461331386749
3	3.8972773937909877	3.6115913525302656
4	1.9081321950385355	2.3576779509596135
5	1.11954745992594	1.7291335790200084
6	0.7705637795311195	1.4281567368857808
7	0.5371528415878905	1.1614805697687087
8	0.41816454785655893	1.0333637647516287
9	0.32547782615985515	0.9048569939661246
>10	4.60492350404678	35.44785596441088
>50	0.501670087592446	9.538561615591343
>100	0.07665278296626855	4.299052013306346
>500	0.004209256741745916	0.9098214850065395
>1k	0.003987716913232972	3.210071923180651
>5k	2.2153982851294297E-4	0.4094367765922622
>10k+	6.646194855388288E-4	4.1970178018391175
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGC	21780	1.4962542120792643	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCG	20199	1.3876418195495437	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTC	19351	1.3293854572059616	No Hit
GCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	5983	0.4110233678085509	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCAGCTTT	4996	0.34321790833553745	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCT	4493	0.3086625424642853	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCT	4473	0.30728857165429524	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTC	4447	0.30550240960130803	No Hit
CTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGCT	3989	0.2740384780525338	TruSeq Adapter, Index 19 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	3834	0.2633902042751102	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3631	0.24944440055371023	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTA	3594	0.2469025545552285	No Hit
CCTGTCTCTTATACACATCTGACGCTGCAGCTTTCGTATGCCGTCTTCTGC	2926	0.20101192950155775	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.4349270249753544E-4	0.0	0.0	0.29114441463691104	0.0
2	3.4349270249753544E-4	0.0	0.0	1.2499012458480319	0.0
3	3.4349270249753544E-4	0.0	0.0	1.778536514991739	0.0
4	3.4349270249753544E-4	0.0	0.0	2.679723969264273	0.0
5	3.4349270249753544E-4	0.0	0.0	4.962232977360396	0.0
6	3.4349270249753544E-4	0.0	0.0	5.71846651117897	0.0
7	3.4349270249753544E-4	0.0	0.0	6.648026462677801	0.0
8	3.4349270249753544E-4	0.0	0.0	7.911049129761238	0.0
9	3.4349270249753544E-4	0.0	0.0	8.38864138331381	0.0
10	3.4349270249753544E-4	0.0	0.0	10.85024748649215	0.0
11	3.4349270249753544E-4	0.0	0.0	12.545383973317486	0.0
12	3.4349270249753544E-4	0.0	0.0	15.741446172976055	0.0
13	3.4349270249753544E-4	0.0	0.0	16.327376024896353	0.0
14	3.4349270249753544E-4	0.0	0.0	16.568164409347123	0.0
15	3.4349270249753544E-4	0.0	0.0	17.10538699605327	0.0
16	3.4349270249753544E-4	0.0	0.0	17.83688905529202	0.0
17	3.4349270249753544E-4	0.0	0.0	18.772357081273807	0.0
18	3.4349270249753544E-4	0.0	0.0	19.77123386013664	0.0
19	3.4349270249753544E-4	0.0	0.0	20.864502433645796	0.0
20	3.4349270249753544E-4	0.0	0.0	21.553274000693854	0.0
21	3.4349270249753544E-4	0.0	0.0	22.469918626578778	0.0
22	3.4349270249753544E-4	0.0	0.0	23.493870372723933	0.0
23	3.4349270249753544E-4	0.0	0.0	24.488693937697292	0.0
24	3.4349270249753544E-4	0.0	0.0	25.26134642269525	0.0
25	3.4349270249753544E-4	0.0	0.0	25.89852538582818	0.0
26	3.4349270249753544E-4	0.0	0.0	26.476074015807534	0.0
27	3.4349270249753544E-4	0.0	0.0	27.058225448000357	0.0
28	3.4349270249753544E-4	0.0	0.0	27.647796322567128	0.0
29	3.4349270249753544E-4	0.0	0.0	28.293081713478998	0.0
30	3.4349270249753544E-4	0.0	0.0	28.9758765075036	0.0
31	3.4349270249753544E-4	0.0	0.0	29.589010981461698	0.0
32	4.1219124299704253E-4	0.0	0.0	30.212381537954226	0.0
33	4.1219124299704253E-4	0.0	0.0	30.835065109041757	0.0
34	4.1219124299704253E-4	0.0	0.0	31.454519848725813	0.0
35	4.1219124299704253E-4	0.0	0.0	32.10083571774518	0.0
36	4.1219124299704253E-4	0.0	0.0	32.6973451449024	0.0
37	4.1219124299704253E-4	0.0	0.0	33.302098396919554	0.0
38	4.1219124299704253E-4	0.0	0.0	33.91221013509568	0.0
39	4.1219124299704253E-4	0.0	0.0	34.52012351997582	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAT	25	3.8913193E-5	45.000004	12
TAGTCCA	25	3.8913193E-5	45.000004	32
ATAGGCG	20	7.033908E-4	45.0	35
TAAGCGT	20	7.033908E-4	45.0	28
GCGATAC	30	2.1659034E-6	44.999996	9
ATGCGAA	80	0.0	42.1875	26
TCGTTCA	395	0.0	42.1519	16
CGATGAA	1205	0.0	41.07884	19
CCGATGA	1180	0.0	40.805084	18
GCGATAT	85	0.0	39.705883	9
CGGGTAT	165	0.0	39.545452	6
TCCGATA	80	0.0	39.375	14
TATGCGT	40	3.459063E-7	39.375	11
CTCGAAT	40	3.459063E-7	39.375	43
TAATACG	115	0.0	39.130432	4
TTTGGGC	4300	0.0	38.668606	4
AACACGT	355	0.0	38.661972	41
GACACGA	385	0.0	38.57143	25
TACGGCT	3100	0.0	38.39516	7
GGCGATA	165	0.0	38.181816	8
>>END_MODULE