FastQCFastQC Report
Sat 18 Jun 2016
SRR3549520_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549520_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242903
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.4293174929982468No Hit
CTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGCT26300.2116013880407401Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC17520.14096031629177821No Hit
TCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC16800.13516742658115719No Hit
CCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC15690.12623672161061644No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA207.033508E-445.031
CGTAAGG556.184564E-1140.909092
GCGTAAG451.9284926E-840.0000041
TATGGGC3700.039.5270274
TACGTTG356.2487015E-638.5714261
TTACGGG3350.038.2835853
TTTGGGA71350.038.283114
TAACGGG1000.038.253
TAGGGAT3700.037.70275
TTTGGGC33150.037.6696854
TTGGGAC26750.037.598135
TTGGGAT35450.037.5740475
GCGATCG301.1399752E-437.5000049
TACGGGA2350.037.3404274
GCGATAG850.037.0588239
TTAACGG552.750312E-936.818182
CGTTTTT26300.036.787071
TGGGCGA22300.036.726466
AATTATC5350.036.58878345
ACGGGAT1800.036.2500045
Produced by FastQC (version 0.11.5)