##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549520_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1242903
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26794448158867	33.0	31.0	34.0	30.0	34.0
2	32.463506001675114	34.0	31.0	34.0	31.0	34.0
3	32.52832119642482	34.0	31.0	34.0	31.0	34.0
4	36.00074905282231	37.0	35.0	37.0	35.0	37.0
5	35.19461373896434	37.0	35.0	37.0	35.0	37.0
6	34.875402988004694	37.0	35.0	37.0	32.0	37.0
7	35.66439698029532	37.0	35.0	37.0	35.0	37.0
8	35.960881098524986	37.0	35.0	37.0	35.0	37.0
9	37.853242771157525	39.0	38.0	39.0	35.0	39.0
10	37.34190761467307	39.0	37.0	39.0	34.0	39.0
11	37.25402786862691	39.0	37.0	39.0	34.0	39.0
12	37.09173282227173	39.0	37.0	39.0	34.0	39.0
13	37.066192615192016	39.0	37.0	39.0	33.0	39.0
14	38.263714062963885	40.0	38.0	41.0	33.0	41.0
15	38.345944132406146	40.0	38.0	41.0	34.0	41.0
16	38.34014400158339	40.0	38.0	41.0	34.0	41.0
17	38.2979001579367	40.0	37.0	41.0	34.0	41.0
18	38.2407887019341	40.0	37.0	41.0	34.0	41.0
19	38.21549388809907	40.0	37.0	41.0	34.0	41.0
20	38.12164746565098	40.0	37.0	41.0	34.0	41.0
21	38.01327698138954	40.0	37.0	41.0	33.0	41.0
22	38.03312969716865	40.0	37.0	41.0	34.0	41.0
23	37.95648011148094	40.0	36.0	41.0	33.0	41.0
24	37.88779977198543	40.0	36.0	41.0	33.0	41.0
25	37.75157755673612	40.0	36.0	41.0	33.0	41.0
26	37.70548626883996	40.0	36.0	41.0	33.0	41.0
27	37.64788563548402	40.0	36.0	41.0	33.0	41.0
28	37.54186529439546	40.0	36.0	41.0	33.0	41.0
29	37.47515051456148	39.0	36.0	41.0	33.0	41.0
30	37.381364434714534	39.0	35.0	41.0	32.0	41.0
31	37.30227700794028	39.0	35.0	41.0	32.0	41.0
32	37.25640697624835	39.0	35.0	41.0	32.0	41.0
33	37.10714754087809	39.0	35.0	41.0	32.0	41.0
34	36.99687827610039	39.0	35.0	41.0	31.0	41.0
35	36.83851515363629	39.0	35.0	41.0	31.0	41.0
36	36.66991470774469	39.0	35.0	41.0	31.0	41.0
37	36.592658477773405	39.0	35.0	41.0	31.0	41.0
38	36.5275922578029	39.0	35.0	41.0	30.0	41.0
39	36.46868661512604	39.0	35.0	41.0	30.0	41.0
40	36.37382482784256	39.0	35.0	41.0	30.0	41.0
41	36.25203093081278	39.0	35.0	41.0	30.0	41.0
42	36.1190929622022	38.0	35.0	40.0	30.0	41.0
43	35.95556290394343	38.0	35.0	40.0	29.0	41.0
44	35.88756403355692	38.0	35.0	40.0	29.0	41.0
45	35.88742967069836	38.0	35.0	40.0	29.0	41.0
46	35.65922441252455	38.0	35.0	40.0	28.0	41.0
47	35.54144691902747	38.0	34.0	40.0	28.0	41.0
48	35.41273293249755	37.0	34.0	40.0	28.0	41.0
49	35.42042621186046	37.0	34.0	40.0	28.0	41.0
50	35.23346874213032	37.0	34.0	40.0	28.0	41.0
51	34.168311605974075	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	16.0
10	19.0
11	22.0
12	28.0
13	22.0
14	26.0
15	45.0
16	77.0
17	160.0
18	249.0
19	538.0
20	901.0
21	1420.0
22	2257.0
23	3326.0
24	4897.0
25	7269.0
26	10364.0
27	12751.0
28	14794.0
29	17149.0
30	21004.0
31	26610.0
32	34180.0
33	47574.0
34	78672.0
35	101599.0
36	111158.0
37	164426.0
38	254787.0
39	326418.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.578026603846	4.115848139396236	54.797759760818025	17.50836549593975
2	20.471991780533155	4.387148474176987	54.8951929474786	20.245666797811253
3	21.063992926238008	4.289393460310258	53.131097116991434	21.5155164964603
4	18.367885506753144	4.792328926714314	52.41229605206521	24.42748951446734
5	17.103828697814713	7.629638032895568	51.221857216532584	24.044676052757133
6	21.68463669328982	7.7676214475305	53.819565967738434	16.728175891441246
7	85.46652474086875	1.813657220233598	8.937543798671337	3.782274240226309
8	88.20495243796177	2.1240595605610415	6.642915818853121	3.0280721826240664
9	83.00945447874855	4.605025492737567	8.143274253903964	4.242245774609925
10	40.15003584350508	28.691297711889018	15.528886807739623	15.629779636866273
11	30.78719739191232	25.03316831643338	23.917795676734226	20.26183861492007
12	25.982397660959865	21.954247435238308	30.490714078250676	21.572640825551147
13	23.702654189425886	22.67465763619526	32.34331239042789	21.27937578395096
14	22.55727116275365	23.639736970624416	31.134368490541902	22.66862337608003
15	20.36812204974966	23.26569330028168	33.68589503766585	22.680289612302808
16	22.645612730840618	22.02512987739188	31.826940638167255	23.50231675360024
17	22.25435130496909	22.22377772038526	29.792188127311626	25.729682847334022
18	22.96196887448176	21.756645530664905	31.1098291660733	24.171556428780043
19	24.079031107013176	23.692597089233832	29.111443129512114	23.11692867424087
20	26.35169438001196	23.623484696714065	28.390630644547482	21.634190278726496
21	26.38588852066493	24.223290152167948	27.804905129362467	21.585916197804657
22	25.09713147365482	22.807974556341083	27.591533691687925	24.50336027831617
23	23.893014981861015	24.33415962468511	27.19528394412114	24.57754144933273
24	23.40898686381801	23.3081744914929	29.222956256441574	24.059882388247512
25	22.988921902996452	25.08989036151655	27.557902748645713	24.36328498684129
26	22.90999378068924	24.75092585664368	27.51373196460223	24.825348398064854
27	22.54053614803408	24.30398832410896	28.53730339374835	24.618172134108615
28	21.429427718816353	24.253139625537955	30.80288646821192	23.514546187433773
29	22.543915333698607	23.178558584217754	29.148211887814252	25.129314194269387
30	23.204867958320158	24.712789332715428	28.516545538951952	23.565797170012463
31	23.884245190493548	26.01160347991758	26.327074598741817	23.777076730847057
32	23.86678606456015	25.950134483543767	26.589605142155097	23.593474309740987
33	23.65446056530558	25.22674738092997	26.27888097462151	24.839911079142944
34	22.845950166666267	24.69701979961429	26.822366668999916	25.634663364719536
35	23.523315978801243	24.274541134746638	27.45676854911445	24.74537433733767
36	24.366020518093528	26.744564941914213	25.792117325326274	23.09729721466599
37	22.433287231586053	28.061642783065132	26.84827375909464	22.65679622625418
38	22.191755913373772	26.767173303145942	27.47084848938332	23.570222294096965
39	21.839596493048933	25.39691351617946	27.64809482316802	25.115395167603587
40	22.842973265009416	24.17517698484918	28.236716783208344	24.74513296693306
41	22.21709980585774	24.574242720469737	27.322325233747126	25.8863322399254
42	22.42709205786775	26.06373948731317	26.934442993540124	24.574725461278955
43	22.10051790043149	25.16407153253311	28.343402502045613	24.39200806498979
44	22.06857655022154	24.29393122391691	28.07588363693707	25.56160858892448
45	22.48123948530175	24.193118851591798	27.153365950520676	26.17227571258578
46	23.196259080555766	24.59186276000621	27.787606917032143	24.424271242405883
47	21.683751668472922	25.458382512553275	28.45354786334895	24.404317955624855
48	21.415106408142872	25.603526582524943	28.700308873661097	24.281058135671085
49	21.811838896518875	24.82470474365256	29.10202968373236	24.261426676096203
50	21.670395839417882	23.72244656260384	28.5343264920915	26.072831105886785
51	21.18894233902404	23.963897424014586	27.164629902735772	27.682530334225596
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	261.0
1	1043.0
2	1825.0
3	5520.0
4	9215.0
5	6173.5
6	3132.0
7	3023.0
8	2914.0
9	2963.0
10	3012.0
11	3105.0
12	3198.0
13	3149.5
14	3101.0
15	3036.0
16	2971.0
17	2895.0
18	2819.0
19	2867.5
20	2916.0
21	3206.5
22	3497.0
23	4145.0
24	4793.0
25	5924.0
26	8478.5
27	9902.0
28	12145.5
29	14389.0
30	16723.5
31	19058.0
32	21138.0
33	23218.0
34	27208.5
35	31199.0
36	33169.0
37	35139.0
38	38480.5
39	41822.0
40	47441.0
41	53060.0
42	58103.5
43	63147.0
44	71280.5
45	79414.0
46	87409.0
47	95404.0
48	103825.5
49	112247.0
50	109463.5
51	106680.0
52	97481.5
53	88283.0
54	80939.0
55	73595.0
56	70118.5
57	66642.0
58	63497.0
59	60352.0
60	57454.0
61	54556.0
62	49744.5
63	44933.0
64	40364.0
65	35795.0
66	30910.5
67	26026.0
68	23228.0
69	20430.0
70	17461.5
71	14493.0
72	11868.0
73	9243.0
74	7631.5
75	4754.0
76	3488.0
77	2724.5
78	1961.0
79	1388.5
80	816.0
81	658.0
82	500.0
83	370.5
84	241.0
85	163.0
86	85.0
87	64.0
88	43.0
89	25.0
90	7.0
91	5.0
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1242903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.31691823298378
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.75849967228173	26.68432075173189
2	8.33723979424946	5.722167526160746
3	2.777123777911728	2.8590698882841536
4	1.3516811191253761	1.855421217683742
5	0.8397827511084381	1.440937800162922
6	0.6443648357149393	1.326756922766376
7	0.5184028396277919	1.245299150147748
8	0.482613504971906	1.324944655060369
9	0.42709084449137763	1.319079742961994
>10	6.718946894835089	50.00473345840386
>50	0.10191199307369193	2.3033857173209653
>100	0.03861960147184463	2.3297437004411568
>500	0.002559130156397088	0.5488841270290118
>1k	9.305927841443958E-4	0.6092414215794021
>5k	2.3264819603609896E-4	0.4260139202657174
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5336	0.4293174929982468	No Hit
CTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGCT	2630	0.2116013880407401	Illumina Single End Adapter 2 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC	1752	0.14096031629177821	No Hit
TCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC	1680	0.13516742658115719	No Hit
CCTGTCTCTTATACACATCTGACGCGAATCCGATCGTATGCCGTCTTCTGC	1569	0.12623672161061644	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6091360307280615E-4	0.0	0.0	0.2302673659971856	0.0
2	1.6091360307280615E-4	0.0	0.0	0.6895147891669744	0.0
3	1.6091360307280615E-4	0.0	0.0	0.9847912508055737	0.0
4	1.6091360307280615E-4	0.0	0.0	1.3663978604927336	0.0
5	1.6091360307280615E-4	0.0	0.0	2.027189571511212	0.0
6	1.6091360307280615E-4	0.0	0.0	2.5707557226911515	0.0
7	1.6091360307280615E-4	0.0	0.0	3.0324168499070323	0.0
8	1.6091360307280615E-4	0.0	0.0	3.7248280839293173	0.0
9	1.6091360307280615E-4	0.0	0.0	4.079883949109464	0.0
10	1.6091360307280615E-4	0.0	0.0	4.802546940509436	0.0
11	1.6091360307280615E-4	0.0	0.0	6.0411794001623615	0.0
12	1.6091360307280615E-4	0.0	0.0	7.217457838624575	0.0
13	1.6091360307280615E-4	0.0	0.0	7.594237040219551	0.0
14	1.6091360307280615E-4	0.0	0.0	7.719427823410194	0.0
15	1.6091360307280615E-4	0.0	0.0	7.912202319891415	0.0
16	1.6091360307280615E-4	0.0	0.0	8.398080944369754	0.0
17	2.4137040460920926E-4	0.0	0.0	9.032000083675074	0.0
18	2.4137040460920926E-4	0.0	0.0	9.714273760703772	0.0
19	2.4137040460920926E-4	0.0	0.0	10.196934113120653	8.045680153640308E-5
20	2.4137040460920926E-4	0.0	0.0	10.704616530815358	8.045680153640308E-5
21	2.4137040460920926E-4	0.0	0.0	11.44562367296563	8.045680153640308E-5
22	3.218272061456123E-4	0.0	0.0	12.218572165325854	8.045680153640308E-5
23	3.218272061456123E-4	0.0	0.0	12.99079654647225	8.045680153640308E-5
24	3.218272061456123E-4	0.0	0.0	13.608543868668754	8.045680153640308E-5
25	3.218272061456123E-4	0.0	0.0	14.150098599810283	8.045680153640308E-5
26	3.218272061456123E-4	0.0	0.0	14.607012775735516	8.045680153640308E-5
27	3.218272061456123E-4	0.0	0.0	15.063766038057675	8.045680153640308E-5
28	3.218272061456123E-4	0.0	0.0	15.558655824308092	8.045680153640308E-5
29	3.218272061456123E-4	0.0	0.0	16.058533932253763	8.045680153640308E-5
30	3.218272061456123E-4	0.0	0.0	16.633800063239047	8.045680153640308E-5
31	3.218272061456123E-4	0.0	0.0	17.149367247484317	8.045680153640308E-5
32	3.218272061456123E-4	0.0	0.0	17.635728612771874	8.045680153640308E-5
33	3.218272061456123E-4	0.0	0.0	18.147514327344933	8.045680153640308E-5
34	3.218272061456123E-4	0.0	0.0	18.665092931628614	8.045680153640308E-5
35	3.218272061456123E-4	0.0	0.0	19.21630247895451	8.045680153640308E-5
36	3.218272061456123E-4	0.0	0.0	19.701859276226706	8.045680153640308E-5
37	3.218272061456123E-4	0.0	0.0	20.20688661947071	8.045680153640308E-5
38	3.218272061456123E-4	0.0	0.0	20.71118985150088	8.045680153640308E-5
39	3.218272061456123E-4	0.0	0.0	21.22032049162324	8.045680153640308E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	20	7.033508E-4	45.0	31
CGTAAGG	55	6.184564E-11	40.90909	2
GCGTAAG	45	1.9284926E-8	40.000004	1
TATGGGC	370	0.0	39.527027	4
TACGTTG	35	6.2487015E-6	38.571426	1
TTACGGG	335	0.0	38.283585	3
TTTGGGA	7135	0.0	38.28311	4
TAACGGG	100	0.0	38.25	3
TAGGGAT	370	0.0	37.7027	5
TTTGGGC	3315	0.0	37.669685	4
TTGGGAC	2675	0.0	37.59813	5
TTGGGAT	3545	0.0	37.574047	5
GCGATCG	30	1.1399752E-4	37.500004	9
TACGGGA	235	0.0	37.340427	4
GCGATAG	85	0.0	37.058823	9
TTAACGG	55	2.750312E-9	36.81818	2
CGTTTTT	2630	0.0	36.78707	1
TGGGCGA	2230	0.0	36.72646	6
AATTATC	535	0.0	36.588783	45
ACGGGAT	180	0.0	36.250004	5
>>END_MODULE