FastQCFastQC Report
Sat 18 Jun 2016
SRR3549519_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549519_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1610098
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52560.3264397570831092No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT43560.27054253840449466Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC36410.226135303565373No Hit
TCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC31370.19483286110534886No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC29570.18365341736962595No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATCG403.4593722E-739.3759
TACTCGA356.249851E-638.57142645
TCGTAAA356.249851E-638.57142615
TTTGGGC46800.037.5480774
CGTATGG900.037.5000042
TTTGGGA89300.037.4664044
TGGGCGA34450.037.358496
ACACGAC8650.037.1965326
TACGGGA2800.036.9642834
CGTTTTT35100.036.7948721
TTGGGAC32300.036.7801865
CACGACC8850.036.6101727
CGGGTAC1600.036.56256
CGACCAA8800.036.562529
AACACGT8150.036.4417241
GGGCGAT52850.036.400197
CGAGACA9100.036.34615322
TTGGGCG28150.036.047965
TTGGGAG52550.036.008565
TCAAGCG9000.036.017
Produced by FastQC (version 0.11.5)