##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549519_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1610098
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.205414825681416	33.0	31.0	34.0	30.0	34.0
2	32.39079546710821	34.0	31.0	34.0	30.0	34.0
3	32.464612091934775	34.0	31.0	34.0	30.0	34.0
4	35.94238984210899	37.0	35.0	37.0	35.0	37.0
5	35.21011826609312	37.0	35.0	37.0	35.0	37.0
6	34.887697519033004	37.0	35.0	37.0	32.0	37.0
7	35.67077283494545	37.0	35.0	37.0	35.0	37.0
8	35.942047005834425	37.0	35.0	37.0	35.0	37.0
9	37.80046369848295	39.0	38.0	39.0	35.0	39.0
10	37.28005251854235	39.0	37.0	39.0	34.0	39.0
11	37.22288022219765	39.0	37.0	39.0	34.0	39.0
12	37.09931196734609	39.0	37.0	39.0	34.0	39.0
13	37.061646558159815	39.0	37.0	39.0	33.0	39.0
14	38.22132317411735	40.0	37.0	41.0	33.0	41.0
15	38.30537644292459	40.0	38.0	41.0	34.0	41.0
16	38.28502426560371	40.0	37.0	41.0	34.0	41.0
17	38.22421740788449	40.0	37.0	41.0	34.0	41.0
18	38.17805189497782	40.0	37.0	41.0	34.0	41.0
19	38.16684636587338	40.0	37.0	41.0	34.0	41.0
20	38.08877906810641	40.0	37.0	41.0	34.0	41.0
21	37.976655458239186	40.0	37.0	41.0	33.0	41.0
22	37.99680888989366	40.0	37.0	41.0	33.0	41.0
23	37.91018310686679	40.0	36.0	41.0	33.0	41.0
24	37.85390454494074	40.0	36.0	41.0	33.0	41.0
25	37.735279467460984	40.0	36.0	41.0	33.0	41.0
26	37.68879596148806	40.0	36.0	41.0	33.0	41.0
27	37.6454979758996	40.0	36.0	41.0	33.0	41.0
28	37.57045161226211	39.0	36.0	41.0	33.0	41.0
29	37.48398979441003	39.0	36.0	41.0	33.0	41.0
30	37.399127879172575	39.0	36.0	41.0	32.0	41.0
31	37.337621064059455	39.0	35.0	41.0	32.0	41.0
32	37.291213950952056	39.0	35.0	41.0	32.0	41.0
33	37.16413162428622	39.0	35.0	41.0	32.0	41.0
34	37.02219554337686	39.0	35.0	41.0	31.0	41.0
35	36.86543303575311	39.0	35.0	41.0	31.0	41.0
36	36.72664521041576	39.0	35.0	41.0	31.0	41.0
37	36.702541708641334	39.0	35.0	41.0	31.0	41.0
38	36.63449057138137	39.0	35.0	41.0	31.0	41.0
39	36.61557619474094	39.0	35.0	41.0	31.0	41.0
40	36.4905111365892	39.0	35.0	41.0	30.0	41.0
41	36.37442006635621	39.0	35.0	40.0	30.0	41.0
42	36.24865815621161	39.0	35.0	40.0	30.0	41.0
43	36.08542709822632	38.0	35.0	40.0	30.0	41.0
44	36.006487182767756	38.0	35.0	40.0	30.0	41.0
45	36.03703066521417	38.0	35.0	40.0	30.0	41.0
46	35.867712400114776	38.0	35.0	40.0	29.0	41.0
47	35.79696391151346	38.0	35.0	40.0	29.0	41.0
48	35.70802957335516	38.0	35.0	40.0	29.0	41.0
49	35.747477482737075	38.0	35.0	40.0	29.0	41.0
50	35.552462645130916	38.0	34.0	40.0	28.0	41.0
51	34.55092236621622	37.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	24.0
10	29.0
11	24.0
12	27.0
13	31.0
14	29.0
15	53.0
16	94.0
17	178.0
18	352.0
19	586.0
20	1057.0
21	1719.0
22	2632.0
23	4060.0
24	6114.0
25	8763.0
26	12897.0
27	15599.0
28	18186.0
29	21500.0
30	26304.0
31	34191.0
32	44086.0
33	62094.0
34	101359.0
35	130051.0
36	143596.0
37	211406.0
38	343032.0
39	419890.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.488447287059543	4.393397172097599	52.649093409221045	18.4690621316218
2	19.566262426262252	4.311911448868329	53.820512788662555	22.301313336206867
3	20.92046571078282	4.505564257579352	52.569346710572894	22.00462332106493
4	19.141940428470814	4.98832990290032	51.266134111091375	24.603595557537492
5	17.890277486215126	7.777973763087713	49.69262740528838	24.63912134540879
6	22.04822315163425	7.370234606837596	52.85268350125272	17.728858740275435
7	83.77216790530763	2.156204156517181	9.560722390810994	4.510905547364198
8	86.01445377858987	2.607543143336617	7.280550624868797	4.097452453204712
9	80.04531401194212	5.301540651562824	9.346573935251145	5.306571401243899
10	36.4517563527189	29.747940808571897	16.999834792664796	16.800468046044404
11	29.830420260133234	25.55055654997398	25.04288558833065	19.57613760156214
12	26.156358184408653	22.34733537958559	30.61335396975836	20.882952466247396
13	24.00220359257635	22.438696278114747	32.31933708382968	21.23976304547922
14	21.392797208617115	24.967175911031504	31.518516264227397	22.121510616123988
15	19.974498446678403	23.88326673283241	33.904395881492924	22.23783893899626
16	22.09263038647337	22.481178164310496	32.911723385781485	22.51446806343465
17	21.66166283046125	22.120703211854185	30.796199982858184	25.42143397482638
18	22.988103829704777	22.191257923430747	30.815950333457963	24.004687913406514
19	24.300570524278648	24.31348899259548	29.536525105925232	21.849415377200643
20	27.488016257395515	24.003818401115957	28.01649340599144	20.491671935497095
21	26.629124438388224	24.33125188653113	27.63987036813908	21.399753306941566
22	24.091639142462135	23.84923153745921	27.73539250405876	24.323736816019895
23	22.953199122041017	25.544159423836316	27.51074779299148	23.991893661131186
24	22.585519639177242	23.53223219953071	29.862033242697027	24.020214918595016
25	22.904444325749115	25.16741216994245	27.77532796140359	24.152815542904843
26	21.604833991471324	26.84929737196121	28.031461438993155	23.514407197574307
27	21.57384208911507	26.016490921670606	28.93358044044524	23.476086548769082
28	20.161692021231005	26.679866691344255	30.000968885123765	23.15747240230098
29	21.07598419475088	26.002827157104726	28.931034011594324	23.990154636550074
30	22.993817767614143	25.12132801854297	29.24430686827758	22.640547345565302
31	22.973570552848336	27.54024910284964	26.96699207129007	22.519188273011952
32	23.489067125106672	27.529566523279946	27.0501547110797	21.931211640533682
33	24.104557610778972	26.221944254324892	26.619746127254366	23.05375200764177
34	22.792028808184348	25.659804558480293	28.378645275008108	23.16952135832726
35	22.664707365638613	26.72557819461921	26.622354664126036	23.98735977561614
36	23.034809061311794	28.394358604258873	26.45838948933543	22.112442845093902
37	23.136914647431396	27.873893390340214	27.838678142572686	21.150513819655696
38	23.433045690386546	26.93786340955644	27.560558425636206	22.068532474420813
39	24.702595742619394	25.28138038802607	26.98413388501818	23.031889984336356
40	25.683529822408325	24.227220951768153	28.063136529577704	22.02611269624582
41	23.134492434621993	25.272312616995983	27.875880847004343	23.717314101377678
42	23.760913931947	26.22778240827577	26.568507010132304	23.44279664964493
43	22.593034709688478	25.410254531090654	28.384234996875968	23.6124757623449
44	22.55521092504928	25.956805113725995	27.855012552030995	23.632971409193726
45	23.110270306527923	24.981025999659646	26.949415501416684	24.959288192395743
46	23.42515797175079	25.60844122531672	27.654714185099294	23.3116866178332
47	21.949906154780642	26.102262098331902	29.276354606986654	22.6714771399008
48	22.12834249840693	25.237345801311474	29.046865470300563	23.587446229981033
49	23.20442606599101	24.543661317509866	29.04059256020441	23.21132005629471
50	22.798984906508796	24.0477908798098	28.13176589251089	25.021458321170513
51	22.291748701010746	23.7052651453514	27.639125071890035	26.36386108174782
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	424.0
1	1213.0
2	2002.0
3	5901.0
4	9800.0
5	6830.5
6	3861.0
7	3732.0
8	3603.0
9	3695.5
10	3788.0
11	3979.0
12	4170.0
13	4169.0
14	4168.0
15	4158.5
16	4149.0
17	3994.0
18	3839.0
19	3979.0
20	4119.0
21	4364.0
22	4609.0
23	5524.5
24	6440.0
25	7851.0
26	12200.0
27	15138.0
28	16533.5
29	17929.0
30	20695.5
31	23462.0
32	27317.0
33	31172.0
34	36125.0
35	41078.0
36	44850.0
37	48622.0
38	53253.5
39	57885.0
40	67691.5
41	77498.0
42	83443.5
43	89389.0
44	99226.5
45	109064.0
46	120838.0
47	132612.0
48	144466.5
49	156321.0
50	153532.0
51	150743.0
52	134915.0
53	119087.0
54	105784.0
55	92481.0
56	87052.5
57	81624.0
58	77856.5
59	74089.0
60	69857.5
61	65626.0
62	57926.5
63	50227.0
64	43566.5
65	36906.0
66	30932.5
67	24959.0
68	20588.5
69	16218.0
70	13750.0
71	11282.0
72	10040.0
73	8798.0
74	7368.5
75	4687.5
76	3436.0
77	2805.5
78	2175.0
79	1646.5
80	1118.0
81	846.0
82	574.0
83	435.0
84	296.0
85	175.5
86	55.0
87	46.5
88	38.0
89	23.0
90	8.0
91	8.0
92	8.0
93	6.5
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1610098.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.337011459433924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.3688862091094	24.695415484881757
2	9.965846211199786	6.445313662690473
3	3.475324532062809	3.371448276557006
4	1.657651854772897	2.1441402809417225
5	0.9409588099985665	1.5213897910889476
6	0.6403292062586605	1.2423799728357918
7	0.47022931138166896	1.0644067441497524
8	0.3665768156399777	0.9483198950490206
9	0.29561935976616827	0.8603501961950194
>10	5.343139561264689	43.850064589909245
>50	0.403637310791512	8.019695942141805
>100	0.06837266442882213	3.9849754381398776
>500	0.0019045296250363858	0.4519239113200869
>1k	0.00133317073752547	1.076475612696075
>5k	1.9045296250363858E-4	0.32370020140343103
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5256	0.3264397570831092	No Hit
CTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGCT	4356	0.27054253840449466	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	3641	0.226135303565373	No Hit
TCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	3137	0.19483286110534886	No Hit
GCTGTCTCTTATACACATCTGACGCAATAGGGGTCGTATGCCGTCTTCTGC	2957	0.18365341736962595	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.210802075401622E-5	0.0	0.0	0.28669062380053884	0.0
2	6.210802075401622E-5	0.0	0.0	0.9552213591967694	0.0
3	6.210802075401622E-5	0.0	0.0	1.3931450135333376	0.0
4	6.210802075401622E-5	0.0	0.0	1.8678987241770377	0.0
5	6.210802075401622E-5	0.0	0.0	2.665489926700114	0.0
6	6.210802075401622E-5	0.0	0.0	3.3305426129341194	0.0
7	6.210802075401622E-5	0.0	0.0	3.930630309459424	0.0
8	6.210802075401622E-5	0.0	0.0	4.869144611073363	0.0
9	6.210802075401622E-5	0.0	0.0	5.405944234450326	0.0
10	6.210802075401622E-5	0.0	0.0	6.313901389853289	0.0
11	6.210802075401622E-5	0.0	0.0	7.7700239364311985	0.0
12	1.2421604150803244E-4	0.0	0.0	9.069882702792004	0.0
13	1.2421604150803244E-4	0.0	0.0	9.516874128158658	0.0
14	1.2421604150803244E-4	0.0	0.0	9.672578936188977	0.0
15	1.2421604150803244E-4	0.0	0.0	9.91635291764849	0.0
16	1.2421604150803244E-4	0.0	0.0	10.481535906510038	0.0
17	1.2421604150803244E-4	0.0	0.0	11.269873013940765	0.0
18	1.2421604150803244E-4	0.0	0.0	12.159694627283557	0.0
19	1.8632406226204865E-4	0.0	0.0	12.749658716425957	0.0
20	1.8632406226204865E-4	0.0	0.0	13.364217581786948	0.0
21	1.8632406226204865E-4	0.0	0.0	14.217333354864113	0.0
22	1.8632406226204865E-4	0.0	0.0	15.151437987004519	0.0
23	1.8632406226204865E-4	0.0	0.0	16.04007954795298	0.0
24	1.8632406226204865E-4	0.0	0.0	16.755626055060002	0.0
25	1.8632406226204865E-4	0.0	0.0	17.376085182392625	0.0
26	1.8632406226204865E-4	0.0	0.0	17.902699090365928	0.0
27	1.8632406226204865E-4	0.0	0.0	18.430306726671297	0.0
28	1.8632406226204865E-4	0.0	0.0	18.974745636601003	0.0
29	1.8632406226204865E-4	0.0	0.0	19.587813909463897	0.0
30	1.8632406226204865E-4	0.0	0.0	20.279510936601376	0.0
31	2.4843208301606487E-4	0.0	0.0	20.893262397692563	0.0
32	2.4843208301606487E-4	0.0	0.0	21.489250964848104	0.0
33	2.4843208301606487E-4	0.0	0.0	22.075240140662245	0.0
34	2.4843208301606487E-4	0.0	0.0	22.655329054504758	0.0
35	2.4843208301606487E-4	0.0	0.0	23.279390447041113	0.0
36	2.4843208301606487E-4	0.0	0.0	23.886744781994636	0.0
37	2.4843208301606487E-4	0.0	0.0	24.46472202313151	0.0
38	2.4843208301606487E-4	0.0	0.0	25.03934543114767	0.0
39	2.4843208301606487E-4	0.0	0.0	25.618378508637363	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATCG	40	3.4593722E-7	39.375	9
TACTCGA	35	6.249851E-6	38.571426	45
TCGTAAA	35	6.249851E-6	38.571426	15
TTTGGGC	4680	0.0	37.548077	4
CGTATGG	90	0.0	37.500004	2
TTTGGGA	8930	0.0	37.466404	4
TGGGCGA	3445	0.0	37.35849	6
ACACGAC	865	0.0	37.19653	26
TACGGGA	280	0.0	36.964283	4
CGTTTTT	3510	0.0	36.794872	1
TTGGGAC	3230	0.0	36.780186	5
CACGACC	885	0.0	36.61017	27
CGGGTAC	160	0.0	36.5625	6
CGACCAA	880	0.0	36.5625	29
AACACGT	815	0.0	36.44172	41
GGGCGAT	5285	0.0	36.40019	7
CGAGACA	910	0.0	36.346153	22
TTGGGCG	2815	0.0	36.04796	5
TTGGGAG	5255	0.0	36.00856	5
TCAAGCG	900	0.0	36.0	17
>>END_MODULE