FastQCFastQC Report
Sat 18 Jun 2016
SRR3549518_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549518_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698465
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210761.2408851521815285No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT35250.2075403378933331No Hit
TCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC23450.13806584180421735No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC22540.1327080628685313No Hit
CCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC22230.130882885428902No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA21940.12917546137247454No Hit
CGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTG17060.10044363587121312No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT115000.043.5130421
TACGCGG750.039.02
GGAACAT25100.038.6354568
CGATAAC356.2500512E-638.5714316
AACTTGA7800.038.0769235
ACGGGAG4400.037.8409085
CGTTATT2800.037.767861
AACACGT22050.037.65306541
TACGGGA3650.037.602744
ACACGTG22200.037.542
GTTTTTT137300.037.3142782
TCAAGCG24050.037.2349317
ACACGAC23650.037.1987326
AGACACG23600.037.18220524
ACGTGAG22250.037.01123444
ACGACCA23650.036.9133228
CACGTGA22350.036.8456443
CGACCAA23650.036.8181829
TTACGCG552.750312E-936.818181
CACGACC23950.036.73277727
Produced by FastQC (version 0.11.5)