##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549518_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1698465
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.21035582128569	33.0	31.0	34.0	30.0	34.0
2	32.43293621004848	34.0	31.0	34.0	31.0	34.0
3	32.45432316827253	34.0	31.0	34.0	30.0	34.0
4	35.97062582979337	37.0	35.0	37.0	35.0	37.0
5	35.181751169438286	37.0	35.0	37.0	35.0	37.0
6	34.87561415748926	37.0	35.0	37.0	32.0	37.0
7	35.640502453686125	37.0	35.0	37.0	33.0	37.0
8	35.91851348129046	37.0	35.0	37.0	35.0	37.0
9	37.792958347684525	39.0	38.0	39.0	35.0	39.0
10	37.37563800254936	39.0	37.0	39.0	35.0	39.0
11	37.308777631567324	39.0	37.0	39.0	34.0	39.0
12	37.14107620704577	39.0	37.0	39.0	34.0	39.0
13	37.098125071755966	39.0	37.0	39.0	33.0	39.0
14	38.21276152290451	40.0	38.0	41.0	33.0	41.0
15	38.31445923230682	40.0	38.0	41.0	33.0	41.0
16	38.312133014221665	40.0	38.0	41.0	33.0	41.0
17	38.222456158943515	40.0	37.0	41.0	33.0	41.0
18	38.1210563656007	40.0	37.0	41.0	33.0	41.0
19	38.03243811323754	40.0	37.0	41.0	34.0	41.0
20	37.86103275604737	40.0	36.0	41.0	33.0	41.0
21	37.71662883839231	40.0	35.0	41.0	33.0	41.0
22	37.77636983982596	40.0	35.0	41.0	33.0	41.0
23	37.68868125042317	39.0	35.0	41.0	33.0	41.0
24	37.62785279649566	39.0	35.0	41.0	33.0	41.0
25	37.52110346695399	39.0	35.0	41.0	33.0	41.0
26	37.47580374043622	39.0	35.0	41.0	33.0	41.0
27	37.434379277759625	39.0	35.0	41.0	33.0	41.0
28	37.31835451422314	39.0	35.0	41.0	33.0	41.0
29	37.221998687049776	39.0	35.0	41.0	33.0	41.0
30	37.08186568460345	39.0	35.0	41.0	32.0	41.0
31	36.910533334510866	39.0	35.0	41.0	31.0	41.0
32	36.71610542460398	39.0	35.0	41.0	31.0	41.0
33	36.444581430880234	39.0	35.0	41.0	30.0	41.0
34	36.13001327669396	39.0	35.0	41.0	30.0	41.0
35	35.85791229139252	39.0	35.0	41.0	29.0	41.0
36	35.6593641906074	38.0	35.0	40.0	27.0	41.0
37	35.615415684161874	38.0	35.0	40.0	27.0	41.0
38	35.47613521620993	38.0	35.0	40.0	27.0	41.0
39	35.42409705233843	38.0	35.0	40.0	27.0	41.0
40	35.28508388456636	38.0	35.0	40.0	26.0	41.0
41	35.13125675242057	38.0	34.0	40.0	25.0	41.0
42	35.0260965047852	38.0	34.0	40.0	25.0	41.0
43	34.87846673319733	38.0	34.0	40.0	24.0	41.0
44	34.7715242881072	37.0	34.0	40.0	24.0	41.0
45	34.74195111468296	37.0	34.0	40.0	24.0	41.0
46	34.53517617378044	37.0	34.0	40.0	23.0	41.0
47	34.44379778211503	37.0	34.0	40.0	23.0	41.0
48	34.36082933707789	37.0	33.0	40.0	23.0	41.0
49	34.38647249133777	36.0	34.0	40.0	24.0	41.0
50	34.2085430079513	36.0	33.0	40.0	23.0	41.0
51	33.235383714118335	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	8.0
9	18.0
10	27.0
11	26.0
12	30.0
13	29.0
14	44.0
15	54.0
16	107.0
17	204.0
18	458.0
19	939.0
20	1599.0
21	2703.0
22	4198.0
23	6283.0
24	10223.0
25	17046.0
26	24813.0
27	28952.0
28	29376.0
29	30079.0
30	33519.0
31	39532.0
32	49680.0
33	68392.0
34	111382.0
35	133958.0
36	158315.0
37	226143.0
38	340801.0
39	379376.0
40	151.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.906860017721883	4.101585843688271	51.94631623259826	21.045237905991588
2	24.164996040542487	4.103175514361497	48.365612479503554	23.36621596559246
3	19.638438236878596	4.682345529639999	53.167713199859875	22.511503033621537
4	17.50627772724195	4.649963349259478	52.4848613306721	25.358897592826462
5	16.836614236972796	7.981442066807381	51.850582732055116	23.331360964164702
6	20.332947691003348	7.510134150541813	53.99581386722717	18.161104291227666
7	79.10654620495565	2.0058700061526142	13.952892759050084	4.934691029841652
8	81.94616904086925	2.282119443144251	12.131012414150424	3.6406991018360695
9	76.487652085854	4.694533004801394	14.1157456880183	4.702069221326315
10	38.00784826299041	27.1171911107971	19.251500619677177	15.623460006535314
11	29.50558298228106	22.603586179285415	28.330639724692592	19.560191113740938
12	24.001201084508658	20.689446058647075	34.01259372433344	21.296759132510825
13	22.36354590762836	21.007203563217374	35.85561080151784	20.773639727636425
14	19.887486642350595	21.117302976511144	37.654705866767934	21.34050451437033
15	19.209580415257303	19.5269257829864	38.024098229872266	23.239395571884025
16	23.243752447062494	20.483966404959773	34.045682425013176	22.226598722964557
17	22.44768069992611	20.399007338979608	33.453559537582464	23.699752423511818
18	24.723029323536252	19.278701651196815	32.80079365780278	23.19747536746415
19	23.569399428307324	22.378912724136203	30.96637257759212	23.085315269964347
20	26.20554441804806	23.714589349795258	29.98071788350069	20.099148348655994
21	25.718987438657848	24.51754966984895	29.54008472355921	20.223378167933987
22	25.13239896023763	21.068140939024353	30.36818539092651	23.431274709811508
23	23.270364711666122	23.824924269855426	30.59073928517809	22.31397173330036
24	22.420244161640067	22.91834097258407	32.048290662451095	22.613124203324766
25	23.83746500516643	24.469035275969773	28.61330672106873	23.080192997795066
26	22.66923369042047	23.359091885908747	29.50322791461702	24.468446509053763
27	22.17784882231898	22.069456833081635	31.051331643572283	24.701362701027104
28	20.36244491349542	24.684170707079627	30.077864424642247	24.875519954782703
29	21.37170916091883	24.239887192258895	29.148790231179326	25.239613415642946
30	23.19729873738935	23.477492912718247	30.756889308875955	22.568319041016448
31	23.736962492603617	25.233195856258444	27.9140282549243	23.11581339621364
32	23.01619403402484	24.564415516363304	27.974671247273275	24.444719202338582
33	22.786045046556744	24.54192462017174	26.21726087967665	26.454769453594864
34	21.15928205762262	26.355326721480864	28.72788076292417	23.757510457972344
35	21.133081929860197	27.83389707765541	27.076212933442847	23.956808059041546
36	22.611593409343143	29.989726017315636	24.13932580300448	23.259354770336746
37	22.705854992596255	27.637719941241063	24.6013900786887	25.055034987473984
38	22.35000426856014	27.030819004218515	26.34172620572105	24.277450521500295
39	23.798959648859412	24.64631299438022	25.10790625653163	26.446821100228735
40	25.110496830962077	24.205620957747144	26.692454657587884	23.991427553702902
41	21.74557615258483	24.86692395780896	26.278728145708037	27.10877174389817
42	23.636636610115605	24.72179291301263	26.64435240054991	24.997218076321857
43	23.05411062341585	23.421618932388952	28.179679887427767	25.344590556767432
44	23.867138857733305	24.13143632632995	27.32449594192403	24.67692887401271
45	23.30186374167263	22.869944332088092	26.476553829487216	27.35163809675207
46	22.522277468184505	23.690979796463278	27.50825009641058	26.278492638941632
47	20.96834494676075	25.778747280632807	29.331307975142263	23.92159979746418
48	21.694765567733217	25.647923271895507	28.22984282867177	24.427468331699504
49	22.794699920222083	25.013644673278517	27.930454851880963	24.261200554618434
50	21.612809213024704	24.400856067095877	28.17496975209969	25.81136496777973
51	20.78894766745267	22.728640272245823	27.946057175155214	28.53635488514629
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	212.0
1	827.0
2	1442.0
3	18194.5
4	34947.0
5	22793.5
6	10640.0
7	10297.0
8	9954.0
9	10073.5
10	10193.0
11	9962.5
12	9732.0
13	9467.5
14	9203.0
15	8579.5
16	7956.0
17	7360.0
18	6764.0
19	6617.0
20	6470.0
21	6461.0
22	6452.0
23	7058.0
24	7664.0
25	7892.0
26	10022.5
27	11925.0
28	13502.0
29	15079.0
30	18252.5
31	21426.0
32	23364.0
33	25302.0
34	29271.5
35	33241.0
36	36311.5
37	39382.0
38	43245.0
39	47108.0
40	51608.0
41	56108.0
42	62620.0
43	69132.0
44	76611.0
45	84090.0
46	99641.0
47	115192.0
48	132111.5
49	149031.0
50	149365.5
51	149700.0
52	139192.0
53	128684.0
54	122266.5
55	115849.0
56	109266.0
57	102683.0
58	97505.5
59	92328.0
60	87113.5
61	81899.0
62	74669.0
63	67439.0
64	59758.0
65	52077.0
66	44191.0
67	36305.0
68	30514.0
69	24723.0
70	20880.5
71	17038.0
72	14403.5
73	11769.0
74	10799.5
75	7855.0
76	5880.0
77	4477.5
78	3075.0
79	2204.5
80	1334.0
81	978.5
82	623.0
83	449.0
84	275.0
85	198.0
86	121.0
87	80.5
88	40.0
89	30.5
90	21.0
91	12.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1698465.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.435340457317295
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.15336175700547	24.37624875100402
2	8.281614575826053	5.372339766063988
3	3.1069898651907715	3.023288222246911
4	1.7463062879833795	2.2656815597397957
5	1.1863905193382311	1.9240490205034502
6	0.9450388965857432	1.8391595013699635
7	0.8156938173748509	1.8520114672837464
8	0.6888492594493236	1.7874448203207765
9	0.6465964004323971	1.8875316947850596
>10	7.22789495279712	45.26625528038056
>50	0.1339480995295089	2.9544086329146775
>100	0.059341578683146066	3.4589759114520544
>500	0.005006923830391771	1.1434854506072378
>1k	0.0027816243502176504	1.5814275665333488
>5k	0.0	0.0
>10k+	1.8544162334784334E-4	1.267692354794495
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21076	1.2408851521815285	No Hit
CTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGCT	3525	0.2075403378933331	No Hit
TCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	2345	0.13806584180421735	No Hit
GCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	2254	0.1327080628685313	No Hit
CCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTGC	2223	0.130882885428902	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	2194	0.12917546137247454	No Hit
CGCTGTCTCTTATACACATCTGACGCAAACACCGTCGTATGCCGTCTTCTG	1706	0.10044363587121312	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1775338320189111E-4	0.0	0.0	0.22314266116758366	0.0
2	1.1775338320189111E-4	0.0	0.0	0.667308422605117	0.0
3	1.1775338320189111E-4	0.0	0.0	1.0410576608879194	0.0
4	1.1775338320189111E-4	0.0	0.0	1.447012449476439	0.0
5	1.1775338320189111E-4	0.0	0.0	2.223772641767714	0.0
6	1.1775338320189111E-4	0.0	0.0	3.255174525233078	0.0
7	1.1775338320189111E-4	0.0	0.0	4.100349433164651	0.0
8	1.1775338320189111E-4	0.0	0.0	5.268109734377806	0.0
9	1.1775338320189111E-4	0.0	0.0	6.125118857321169	0.0
10	1.1775338320189111E-4	0.0	0.0	7.219930937640752	0.0
11	1.1775338320189111E-4	0.0	0.0	8.560788712160686	0.0
12	1.1775338320189111E-4	0.0	0.0	9.650831780460592	0.0
13	1.1775338320189111E-4	0.0	0.0	10.098647896777385	0.0
14	1.1775338320189111E-4	0.0	0.0	10.299005278295402	0.0
15	1.1775338320189111E-4	0.0	0.0	10.54858357399181	0.0
16	1.1775338320189111E-4	0.0	0.0	10.920684264909786	0.0
17	1.1775338320189111E-4	0.0	0.0	11.422902444265851	0.0
18	1.1775338320189111E-4	0.0	0.0	11.996714680608667	0.0
19	1.1775338320189111E-4	0.0	0.0	12.377116985042376	0.0
20	1.1775338320189111E-4	0.0	0.0	12.802265575092804	0.0
21	1.1775338320189111E-4	0.0	0.0	13.335747277688972	0.0
22	1.1775338320189111E-4	0.0	0.0	13.897784175711598	0.0
23	1.1775338320189111E-4	0.0	0.0	14.432855548980992	0.0
24	1.1775338320189111E-4	0.0	0.0	14.868248683369984	0.0
25	1.1775338320189111E-4	0.0	0.0	15.304760474899394	0.0
26	1.1775338320189111E-4	0.0	0.0	15.647952710241306	0.0
27	1.1775338320189111E-4	0.0	0.0	16.025941070319377	0.0
28	7.065202992113468E-4	0.0	0.0	16.389622394338417	0.0
29	7.065202992113468E-4	0.0	0.0	16.812415916724806	0.0
30	7.065202992113468E-4	0.0	0.0	17.367681995213324	0.0
31	7.065202992113468E-4	0.0	0.0	17.746141368824205	0.0
32	7.065202992113468E-4	0.0	0.0	18.186539021999277	0.0
33	7.065202992113468E-4	0.0	0.0	18.58884345570854	0.0
34	7.653969908122922E-4	0.0	0.0	19.025649630695952	0.0
35	8.242736824132378E-4	0.0	0.0	19.523275428107144	0.0
36	8.831503740141834E-4	0.0	0.0	19.93211517458411	0.0
37	8.831503740141834E-4	0.0	0.0	20.35920669545737	0.0
38	8.831503740141834E-4	0.0	0.0	20.842231073351527	0.0
39	8.831503740141834E-4	0.0	0.0	21.54103852596315	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	11500	0.0	43.513042	1
TACGCGG	75	0.0	39.0	2
GGAACAT	2510	0.0	38.635456	8
CGATAAC	35	6.2500512E-6	38.57143	16
AACTTGA	780	0.0	38.076923	5
ACGGGAG	440	0.0	37.840908	5
CGTTATT	280	0.0	37.76786	1
AACACGT	2205	0.0	37.653065	41
TACGGGA	365	0.0	37.60274	4
ACACGTG	2220	0.0	37.5	42
GTTTTTT	13730	0.0	37.314278	2
TCAAGCG	2405	0.0	37.23493	17
ACACGAC	2365	0.0	37.19873	26
AGACACG	2360	0.0	37.182205	24
ACGTGAG	2225	0.0	37.011234	44
ACGACCA	2365	0.0	36.91332	28
CACGTGA	2235	0.0	36.84564	43
CGACCAA	2365	0.0	36.81818	29
TTACGCG	55	2.750312E-9	36.81818	1
CACGACC	2395	0.0	36.732777	27
>>END_MODULE