FastQCFastQC Report
Sat 18 Jun 2016
SRR3549515_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549515_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1479689
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC65740.4442825485625696Illumina PCR Primer Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGCT63500.42914423233530835TruSeq Adapter, Index 23 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC55970.3782551603749166Illumina PCR Primer Index 7 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52020.3515603616705943No Hit
CCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTGC40920.276544598223005Illumina PCR Primer Index 7 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG24640.16652147849987395TruSeq Adapter, Index 23 (95% over 24bp)
GCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCTG17960.12137685689357697Illumina PCR Primer Index 7 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCCGTCTTCT17930.12117411158696186Illumina PCR Primer Index 7 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAAGTCTTCTCGTATGCC15370.10387317875580612No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG253.8913517E-545.016
GTCGTAG253.8913517E-545.01
TAGTCGT207.033945E-445.015
TACGTAG207.033945E-445.01
CGTTTTT39900.041.447371
CGTTGAT603.6379788E-1241.25000425
TCGTTCA1050.040.71428316
TAGCGCG501.0822987E-940.51
GCTACGA1250.039.60000210
GCGATCG403.4591176E-739.3759
CTATGCG356.2495055E-638.5714261
CGGCCTA356.2495055E-638.57142644
CGTACAT356.2495055E-638.57142635
ATCGGAT1050.038.57142613
CGATAAC356.2495055E-638.57142616
CTACGAA1350.038.33333211
TGGGCGC2950.038.1355936
AGCTACG1300.038.076929
GACCGAT6800.038.051479
TCCGATA301.1400803E-437.50000414
Produced by FastQC (version 0.11.5)