FastQCFastQC Report
Sat 18 Jun 2016
SRR3549512_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549512_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874857
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC25080.2866754223833152No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC21020.2402678380581055No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20560.23500983589318022No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT19070.21797848105461806No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC18730.214092131628369No Hit
TCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG8760.10013064992335892No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGTC351.2114833E-745.00000412
CTATCGT253.8900384E-545.017
ATCGTAG253.8900384E-545.022
ACGATAG253.8900384E-545.029
GCGATCG207.0323615E-445.09
CGGTCGT207.0323615E-445.023
CGATAGT302.1649048E-644.99999610
TACGGGA1650.043.6363644
CAACGAT750.042.026
TACAACG750.042.024
CGTTTTT13300.040.263161
CGTTAGG850.039.7058832
TTTAGCG800.039.3751
AATGCGA800.039.37525
ACGGGAT1150.039.1304365
ATGGGCG2400.038.4374965
TGGGCGA18650.037.5201076
CAAACGG301.13969916E-437.4999964
CAACCCG601.546141E-1037.49999623
AACGCGT301.13969916E-437.49999636