##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549512_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	874857
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.210844743769556	33.0	31.0	34.0	30.0	34.0
2	32.405177074653345	34.0	31.0	34.0	30.0	34.0
3	32.470491748937256	34.0	31.0	34.0	30.0	34.0
4	35.94675472677249	37.0	35.0	37.0	35.0	37.0
5	35.1619441805918	37.0	35.0	37.0	35.0	37.0
6	34.814150198260975	37.0	35.0	37.0	32.0	37.0
7	35.64582554634643	37.0	35.0	37.0	35.0	37.0
8	35.92111739404268	37.0	35.0	37.0	35.0	37.0
9	37.78555923996722	39.0	38.0	39.0	35.0	39.0
10	37.258278781560875	39.0	37.0	39.0	34.0	39.0
11	37.17542524092509	39.0	37.0	39.0	34.0	39.0
12	36.95066850925351	39.0	37.0	39.0	33.0	39.0
13	36.871848770713385	39.0	37.0	39.0	33.0	39.0
14	37.97524738328664	40.0	37.0	41.0	33.0	41.0
15	38.07897404947323	40.0	37.0	41.0	33.0	41.0
16	38.07696457821107	40.0	37.0	41.0	33.0	41.0
17	38.03908067261278	40.0	37.0	41.0	33.0	41.0
18	37.994368222463784	40.0	37.0	41.0	33.0	41.0
19	37.99810369008878	40.0	37.0	41.0	34.0	41.0
20	37.92186951696106	40.0	36.0	41.0	33.0	41.0
21	37.8137044111209	40.0	36.0	41.0	33.0	41.0
22	37.83950177000356	40.0	36.0	41.0	33.0	41.0
23	37.742471055269604	40.0	36.0	41.0	33.0	41.0
24	37.685413730472526	39.0	36.0	41.0	33.0	41.0
25	37.598335499401614	39.0	35.0	41.0	33.0	41.0
26	37.541081571045325	39.0	35.0	41.0	33.0	41.0
27	37.4933411974757	39.0	35.0	41.0	33.0	41.0
28	37.41813690694593	39.0	35.0	41.0	33.0	41.0
29	37.37286322221803	39.0	35.0	41.0	33.0	41.0
30	37.283215428350005	39.0	35.0	41.0	32.0	41.0
31	37.20137919682874	39.0	35.0	41.0	32.0	41.0
32	37.176474555270175	39.0	35.0	41.0	32.0	41.0
33	37.059075940410835	39.0	35.0	41.0	32.0	41.0
34	36.95744104465073	39.0	35.0	41.0	31.0	41.0
35	36.84295376272922	39.0	35.0	41.0	31.0	41.0
36	36.71524031927503	39.0	35.0	41.0	31.0	41.0
37	36.69591487523104	39.0	35.0	41.0	31.0	41.0
38	36.597288471144424	39.0	35.0	41.0	31.0	41.0
39	36.57989705746196	39.0	35.0	41.0	31.0	41.0
40	36.49298913993944	39.0	35.0	41.0	30.0	41.0
41	36.38222932433529	39.0	35.0	40.0	30.0	41.0
42	36.30011533313444	38.0	35.0	40.0	30.0	41.0
43	36.163206101111385	38.0	35.0	40.0	30.0	41.0
44	36.09917163605024	38.0	35.0	40.0	30.0	41.0
45	36.11130047539198	38.0	35.0	40.0	30.0	41.0
46	35.91767111653676	38.0	35.0	40.0	30.0	41.0
47	35.84264971303882	38.0	35.0	40.0	30.0	41.0
48	35.774695750276905	38.0	35.0	40.0	29.0	41.0
49	35.84053393868941	38.0	35.0	40.0	30.0	41.0
50	35.6597969725338	38.0	35.0	40.0	29.0	41.0
51	34.59839150855511	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	16.0
11	13.0
12	22.0
13	22.0
14	14.0
15	26.0
16	54.0
17	102.0
18	201.0
19	376.0
20	560.0
21	1006.0
22	1648.0
23	2364.0
24	3389.0
25	4811.0
26	6760.0
27	8493.0
28	9769.0
29	11626.0
30	14784.0
31	18926.0
32	24743.0
33	34951.0
34	60788.0
35	77897.0
36	74188.0
37	108825.0
38	179765.0
39	228619.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.125657107390122	4.616182987619691	54.40854905430259	16.8496108506876
2	21.659997005224856	4.855764999308458	51.19819581943106	22.286042176035625
3	21.370806886153964	4.85850830478581	51.061487763143006	22.709197045917218
4	19.345218704313965	4.8307323368276185	50.342856032471595	25.481192926386825
5	17.962021221753954	8.218485992567928	48.11071980906594	25.708772976612178
6	22.46961503422845	8.142359265571402	52.055821694288326	17.332204005911823
7	84.2202782854798	2.06959537387253	9.351471154714428	4.358655185933244
8	85.91404080895506	2.9085896323627747	7.030749025269273	4.146620533412889
9	80.04976813353497	5.355961031345694	8.982953785590102	5.611317049529237
10	38.47885997368713	29.024972081151546	16.315695022157904	16.180472923003418
11	32.369061458044	24.266823035078875	24.977453458108013	18.38666204876911
12	27.989717176635725	22.45235507060011	30.41388478345604	19.144042969308128
13	25.475706315432124	23.494011021229756	31.84554732944927	19.184735333888852
14	21.708576373052967	26.084605827009444	31.03912982350258	21.16768797643501
15	20.304918403807708	23.351816353986994	35.80985235301312	20.533412889192178
16	21.679885969935658	22.097782837652325	33.95594937229742	22.2663818201146
17	22.700166998720935	20.700411610126	30.893048806833573	25.706372584319492
18	23.016904476960235	21.988507836137792	31.377356527981142	23.61723115892083
19	25.040663788482004	23.70936050120191	29.522424807711435	21.727550902604655
20	27.729674678261706	24.22613067049815	27.680638092854032	20.363556558386115
21	27.309834635831916	24.248305723106746	27.882956871808762	20.558902769252573
22	24.648942627195073	23.89544805608231	27.51443950268444	23.94116981403818
23	22.837789490168106	25.91189188633114	27.401963978112992	23.848354645387758
24	23.319696819022994	23.726048942855805	29.30627519697505	23.647979041146154
25	23.341986176026484	23.934883072319245	27.615941805346473	25.107188946307797
26	21.1234521756127	26.204625441643604	27.87747026085406	24.794452121889634
27	21.46487940314817	25.612414371720178	30.21099448252686	22.71171174260479
28	19.714764812992293	25.645448341843295	31.393816360845257	23.24597048431915
29	20.952452800857742	24.7527310177549	29.611353626935603	24.683462554451758
30	22.489389694544364	24.95253510002206	29.396689973332784	23.16138523210079
31	22.519108837215683	26.92725782613616	27.243080869216342	23.31055246743182
32	23.4558333533366	26.464782244412515	26.67395928706063	23.405425115190255
33	23.05325327453515	26.411516396393925	26.511532741922395	24.02369758714853
34	21.35903353348033	25.59412566853783	28.31914244270778	24.72769835527406
35	20.391218221949416	24.958136015371654	28.283022253922642	26.36762350875629
36	21.86517339405183	27.024759474977056	27.64977590623382	23.4602912247373
37	20.780310382153882	27.94948202963456	28.488998773513842	22.781208814697717
38	20.756877981201498	28.114308967065476	26.24337463151121	24.88543842022182
39	21.47185197123644	26.6875615100525	27.398191933081634	24.442394585629422
40	22.301587573740623	24.71741095973399	29.444697819186448	23.536303647338936
41	19.636694911282643	24.661516110632938	28.86025944811552	26.8415295299689
42	21.070186327594108	25.534115861220748	27.288459713987546	26.107238097197598
43	21.56158092122484	25.381976711622585	27.42151002963913	25.634932337513444
44	21.478367321745154	25.24046787074916	27.84889416213164	25.432270645374043
45	21.4512771801563	24.811254867938416	27.12797634356243	26.60949160834285
46	21.649938218474563	25.562806264338057	28.010520576505648	24.776734940681735
47	19.287609289289563	26.356307373662208	29.404119759000615	24.95196357804761
48	19.43529056748703	25.20217589846112	29.183740885653314	26.178792648398534
49	20.94147957894833	23.86652904417522	30.24631454054777	24.94567683632868
50	20.22216202190758	23.972374913843062	28.904495248937828	26.90096781531153
51	19.401227857809904	25.02454686880256	27.147179481903898	28.427045791483636
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	138.0
1	564.0
2	990.0
3	2536.5
4	4083.0
5	2860.0
6	1637.0
7	1625.0
8	1613.0
9	1655.0
10	1697.0
11	1738.5
12	1780.0
13	1768.0
14	1756.0
15	1773.5
16	1791.0
17	1869.0
18	1947.0
19	1996.5
20	2046.0
21	2470.5
22	2895.0
23	3202.0
24	3509.0
25	3714.5
26	5336.0
27	6752.0
28	8040.5
29	9329.0
30	10627.5
31	11926.0
32	13427.5
33	14929.0
34	17855.5
35	20782.0
36	21716.0
37	22650.0
38	26899.5
39	31149.0
40	36532.5
41	41916.0
42	49150.0
43	56384.0
44	65573.0
45	74762.0
46	81170.0
47	87578.0
48	86903.5
49	86229.0
50	77576.0
51	68923.0
52	63400.0
53	57877.0
54	54010.5
55	50144.0
56	47014.0
57	43884.0
58	41827.5
59	39771.0
60	37559.5
61	35348.0
62	31156.5
63	26965.0
64	23795.0
65	20625.0
66	16838.5
67	13052.0
68	10990.5
69	8929.0
70	7471.0
71	6013.0
72	4866.5
73	3720.0
74	3232.0
75	1981.5
76	1219.0
77	1008.0
78	797.0
79	592.0
80	387.0
81	295.5
82	204.0
83	119.5
84	35.0
85	25.0
86	15.0
87	12.0
88	9.0
89	6.5
90	4.0
91	3.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	874857.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.158923598564453
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.13137455482301	23.410127596088593
2	10.79221061563063	6.72547332064102
3	3.853082151992579	3.6017367717878717
4	1.7783401254460245	2.216446564041368
5	1.0204217378996299	1.5897621484764461
6	0.6420796000962541	1.2003905522157599
7	0.4573934365980973	0.9976321001811462
8	0.34942119631240576	0.8710070687693793
9	0.3052584450833232	0.8560372111351066
>10	4.918517022267868	40.514237990431
>50	0.6821668955243508	12.95476181210033
>100	0.0653984120050501	3.311728239009628
>500	0.0025292203537864593	0.5746213262137987
>1k	0.0018065859669903282	1.1760372989085701
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	2508	0.2866754223833152	No Hit
GCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	2102	0.2402678380581055	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2056	0.23500983589318022	No Hit
CTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGCT	1907	0.21797848105461806	No Hit
CCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTGC	1873	0.214092131628369	No Hit
TCCTGTCTCTTATACACATCTGACGCCTACATCATCGTATGCCGTCTTCTG	876	0.10013064992335892	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.5721757955871644E-4	0.0	0.0	0.2314663996516002	0.0
2	4.5721757955871644E-4	0.0	0.0	1.0473711703741297	0.0
3	4.5721757955871644E-4	0.0	0.0	1.5731713868666537	0.0
4	4.5721757955871644E-4	0.0	0.0	2.162639151312729	0.0
5	4.5721757955871644E-4	0.0	0.0	3.1093081497890513	0.0
6	4.5721757955871644E-4	0.0	0.0	3.7336387546764787	0.0
7	4.5721757955871644E-4	0.0	0.0	4.277041847982013	0.0
8	4.5721757955871644E-4	0.0	0.0	5.199364010346834	0.0
9	4.5721757955871644E-4	0.0	0.0	5.69247316990091	0.0
10	4.5721757955871644E-4	0.0	0.0	6.655944914426015	0.0
11	4.5721757955871644E-4	0.0	0.0	8.205912509130064	0.0
12	4.5721757955871644E-4	0.0	0.0	9.66032162970634	0.0
13	4.5721757955871644E-4	0.0	0.0	10.10942359722789	0.0
14	4.5721757955871644E-4	0.0	0.0	10.26270579077495	0.0
15	4.5721757955871644E-4	0.0	0.0	10.532464162714593	0.0
16	4.5721757955871644E-4	0.0	0.0	11.12787575569493	0.0
17	4.5721757955871644E-4	0.0	0.0	11.992931416220022	0.0
18	4.5721757955871644E-4	0.0	0.0	12.919254232405981	0.0
19	4.5721757955871644E-4	0.0	0.0	13.554443754807929	0.0
20	4.5721757955871644E-4	0.0	0.0	14.215123157270273	0.0
21	4.5721757955871644E-4	0.0	0.0	15.053431589391181	0.0
22	5.715219744483955E-4	0.0	0.0	15.901112981893041	0.0
23	6.858263693380747E-4	0.0	0.0	16.713131403189323	0.0
24	6.858263693380747E-4	0.0	0.0	17.339519487184763	0.0
25	6.858263693380747E-4	0.0	0.0	17.90384028475511	0.0
26	6.858263693380747E-4	0.0	0.0	18.398778314627418	0.0
27	6.858263693380747E-4	0.0	0.0	18.86834076883422	0.0
28	6.858263693380747E-4	0.0	0.0	19.38533954692024	0.0
29	6.858263693380747E-4	0.0	0.0	19.957090130158413	0.0
30	6.858263693380747E-4	0.0	0.0	20.570447513136433	0.0
31	6.858263693380747E-4	0.0	0.0	21.171917239045925	0.0
32	6.858263693380747E-4	0.0	0.0	21.751897738716156	0.0
33	6.858263693380747E-4	0.0	0.0	22.296672484760368	0.0
34	6.858263693380747E-4	0.0	0.0	22.861679108700052	0.0
35	6.858263693380747E-4	0.0	0.0	23.453318656649028	0.0
36	6.858263693380747E-4	0.0	0.0	23.99374983568743	0.0
37	6.858263693380747E-4	0.0	0.0	24.551898195933735	0.0
38	6.858263693380747E-4	0.0	0.0	25.111989730893164	0.0
39	6.858263693380747E-4	0.0	0.0	25.706258279924604	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGCGTC	35	1.2114833E-7	45.000004	12
CTATCGT	25	3.8900384E-5	45.0	17
ATCGTAG	25	3.8900384E-5	45.0	22
ACGATAG	25	3.8900384E-5	45.0	29
GCGATCG	20	7.0323615E-4	45.0	9
CGGTCGT	20	7.0323615E-4	45.0	23
CGATAGT	30	2.1649048E-6	44.999996	10
TACGGGA	165	0.0	43.636364	4
CAACGAT	75	0.0	42.0	26
TACAACG	75	0.0	42.0	24
CGTTTTT	1330	0.0	40.26316	1
CGTTAGG	85	0.0	39.705883	2
TTTAGCG	80	0.0	39.375	1
AATGCGA	80	0.0	39.375	25
ACGGGAT	115	0.0	39.130436	5
ATGGGCG	240	0.0	38.437496	5
TGGGCGA	1865	0.0	37.520107	6
CAAACGG	30	1.13969916E-4	37.499996	4
CAACCCG	60	1.546141E-10	37.499996	23
AACGCGT	30	1.13969916E-4	37.499996	36
>>END_MODULE