FastQCFastQC Report
Sat 18 Jun 2016
SRR3549511_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549511_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054206
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89690.43661638608786074No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC59440.2893575425249464No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT56980.27738211260214407Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC48570.23644172006118178No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC43690.21268558265334636No Hit
TCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG25770.12544993053277032No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCA207.0345856E-445.023
TAGCGCG207.0345856E-445.01
CCCGATA207.0345856E-445.016
CTTTACG900.042.5000041
CGTTTTT52400.042.3377881
CTCGCAC451.929584E-840.00000414
TAGGGCG2100.038.571435
CGTTTGG13800.038.1521762
TGGGCGA33750.038.0666666
TTTGGGA160550.038.0208664
AGGGCGA2800.037.7678576
TTGGGAT101650.037.6291165
TTGGGAC60700.037.475295
ACGGGAC2300.037.1739125
TATGGGA18000.037.04
CGACGGT552.752131E-936.8181828
TTTGGGC44450.036.7997744
TACGGGA4350.036.724144
TTTTGCG16500.036.5454521
TTGGGCG27500.036.2454535