##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549511_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2054206
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.169539471698556	33.0	31.0	34.0	30.0	34.0
2	32.36455691396092	34.0	31.0	34.0	30.0	34.0
3	32.41844634861353	34.0	31.0	34.0	30.0	34.0
4	35.93137786570578	37.0	35.0	37.0	35.0	37.0
5	35.15474640810123	37.0	35.0	37.0	35.0	37.0
6	34.89761104777223	37.0	35.0	37.0	32.0	37.0
7	35.71836709658135	37.0	35.0	37.0	35.0	37.0
8	36.00649301968741	37.0	35.0	37.0	35.0	37.0
9	37.88062833036219	39.0	38.0	39.0	35.0	39.0
10	37.25779206175038	39.0	37.0	39.0	34.0	39.0
11	37.032015289605816	39.0	37.0	39.0	33.0	39.0
12	37.0642248148433	39.0	37.0	39.0	33.0	39.0
13	37.10470176798238	39.0	37.0	39.0	33.0	39.0
14	38.25518132066599	40.0	38.0	41.0	33.0	41.0
15	38.329786788666766	40.0	38.0	41.0	34.0	41.0
16	38.301484855949205	40.0	38.0	41.0	34.0	41.0
17	38.2509733687858	40.0	37.0	41.0	34.0	41.0
18	38.202583382581885	40.0	37.0	41.0	34.0	41.0
19	38.20729323154542	40.0	37.0	41.0	34.0	41.0
20	38.108269569848396	40.0	37.0	41.0	34.0	41.0
21	37.997777243372866	40.0	37.0	41.0	33.0	41.0
22	38.04713256606202	40.0	37.0	41.0	34.0	41.0
23	37.96351972489614	40.0	37.0	41.0	33.0	41.0
24	37.90388354429887	40.0	37.0	41.0	33.0	41.0
25	37.81926739577238	40.0	36.0	41.0	33.0	41.0
26	37.80490564237472	40.0	36.0	41.0	33.0	41.0
27	37.79254953008608	40.0	36.0	41.0	33.0	41.0
28	37.708379782748175	40.0	36.0	41.0	33.0	41.0
29	37.677555707655415	40.0	36.0	41.0	33.0	41.0
30	37.60327007125868	40.0	36.0	41.0	33.0	41.0
31	37.53488209069587	40.0	36.0	41.0	33.0	41.0
32	37.49161087057481	40.0	36.0	41.0	33.0	41.0
33	37.36561620402238	40.0	36.0	41.0	32.0	41.0
34	37.27078150876786	40.0	36.0	41.0	32.0	41.0
35	37.14788341578206	40.0	36.0	41.0	31.0	41.0
36	37.04541414054871	39.0	36.0	41.0	31.0	41.0
37	37.02366559147427	39.0	36.0	41.0	31.0	41.0
38	36.9321961867505	39.0	35.0	41.0	31.0	41.0
39	36.88136340756477	39.0	35.0	41.0	31.0	41.0
40	36.84210979814098	39.0	35.0	41.0	31.0	41.0
41	36.73746206563509	39.0	35.0	41.0	31.0	41.0
42	36.63689522861875	39.0	35.0	41.0	30.0	41.0
43	36.49015239951592	39.0	35.0	41.0	30.0	41.0
44	36.459626249752944	39.0	35.0	41.0	30.0	41.0
45	36.496280314632514	39.0	35.0	41.0	30.0	41.0
46	36.30166692142852	39.0	35.0	40.0	30.0	41.0
47	36.236429063102726	39.0	35.0	40.0	30.0	41.0
48	36.15352160396766	39.0	35.0	40.0	29.0	41.0
49	36.216548389012594	39.0	35.0	40.0	30.0	41.0
50	36.04027930986474	39.0	35.0	40.0	29.0	41.0
51	35.017393094947636	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	15.0
11	28.0
12	23.0
13	19.0
14	21.0
15	38.0
16	79.0
17	130.0
18	238.0
19	552.0
20	1049.0
21	1771.0
22	2941.0
23	4663.0
24	7208.0
25	11338.0
26	16166.0
27	19781.0
28	22746.0
29	26618.0
30	32763.0
31	41595.0
32	53519.0
33	73580.0
34	115024.0
35	145855.0
36	171040.0
37	257094.0
38	452007.0
39	596092.0
40	204.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.94015595320041	4.032653005589507	57.5151177632623	16.51207327794778
2	19.650560849301385	3.9762321792458986	54.83641854809109	21.53678842336163
3	18.440360898566162	4.22971211261188	55.297959406213394	22.031967582608562
4	17.013580916422207	4.231367253332918	54.66238536933492	24.092666460909957
5	16.554863533647552	7.141445405183316	53.31797297836731	22.985718082801824
6	21.168860377196836	6.605471895223751	55.040682385310916	17.184985342268497
7	85.07184771147587	1.2220293388296988	9.52937534015576	4.1767476095386735
8	88.02617653730931	1.4337413092941993	6.8470250792763725	3.693057074120122
9	82.68026673079525	4.612293022218804	8.615688981533498	4.091751265452443
10	37.60645232269792	35.81821881544499	14.238883539430807	12.33644532242628
11	22.590285492302133	26.269273870293436	32.487832281669895	18.652608355734525
12	21.71286618771438	23.58054644957711	34.4705935042542	20.23599385845431
13	21.888262423534933	23.975102789106835	35.08474807297807	19.051886714380153
14	19.748944360984243	26.239432656705315	33.33857461228328	20.673048370027153
15	17.861305049250173	26.02358283443822	35.51790813579554	20.597203980516074
16	20.34090057180244	25.181408291086676	33.898645023916785	20.5790461131941
17	20.35199975075528	24.20492394628387	32.27183641757448	23.171239885386374
18	20.458561604824443	24.093834795536573	33.13869202991326	22.308911569725723
19	21.616624622846977	25.140906024030695	32.088651284243156	21.15381806887917
20	23.526705695533945	26.635741498174966	30.410484634939245	19.42706817135185
21	23.264122488202254	26.692016282690247	30.683388131472693	19.360473097634802
22	22.042677316685864	24.513705051976288	30.530628379042803	22.912989252295045
23	20.072427010728234	26.5155977540714	30.12141917607095	23.290556059129415
24	20.46294285967425	25.352812716932966	31.700082659674834	22.484161763717953
25	20.09793565007599	26.543443062672388	29.548789167201345	23.809832120050277
26	19.106408996955516	27.6865124529867	29.392621772110488	23.814456777947292
27	19.396204664965442	25.976460004498087	31.22057865666832	23.406756673868152
28	17.82907848579938	25.607120220659468	32.70149147651209	23.86230981702906
29	18.53942593878121	24.48693071678303	32.32119855554896	24.6524447888868
30	20.468395087931786	24.416100430044505	32.042599427710755	23.072905054312955
31	20.630939642859577	26.70783748075899	29.16732791161159	23.493894964769844
32	20.13050297779288	27.426509317955457	29.023525391319076	23.419462312932588
33	19.855798298710063	26.08613741757156	29.971969705083133	24.086094578635247
34	19.479497187721194	24.733205919951555	30.86662194541346	24.920674946913795
35	18.5344605166181	25.36994829145665	29.769263647365452	26.326327544559796
36	19.064300269787935	25.072120322888747	30.997670146032092	24.865909261291225
37	18.55553922050661	26.409717428534428	31.703538982945233	23.331204368013726
38	19.25327839564289	27.25559169820359	31.32913641572462	22.161993490428905
39	19.238236087325227	26.121966346121077	31.887551686637078	22.752245879916618
40	20.861198925521588	24.253604555726156	31.294086376926174	23.59111014182609
41	19.661465305816456	24.53872688522962	30.04523402229377	25.754573786660153
42	20.966689806183023	24.635260533753677	29.807331883949324	24.590717776113983
43	20.03869134838473	24.411232369100276	30.99883848065871	24.55123780185629
44	19.325179655789146	25.1026917456185	30.494263963789415	25.07786463480294
45	20.252399223836363	24.071052270317583	29.122931195800227	26.55361731004583
46	20.630501517374597	24.067157821562198	30.208460105753755	25.09388055530945
47	18.48719164484964	24.946232266870997	31.455559958446234	25.111016129833132
48	18.056222209457083	24.569687752834916	32.34539281844177	25.028697219266228
49	19.58683793154143	23.298247595421294	32.96767704894251	24.147237424094758
50	19.423903931738103	23.05333544931716	31.37762230272913	26.14513831621561
51	18.577883620240616	22.637749086508364	30.231291311582186	28.55307598166883
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	156.0
1	778.5
2	1401.0
3	8443.5
4	15486.0
5	10335.5
6	5185.0
7	5102.5
8	5020.0
9	5001.5
10	4983.0
11	4916.5
12	4850.0
13	4856.5
14	4863.0
15	4864.5
16	4866.0
17	5055.5
18	5245.0
19	5288.0
20	5331.0
21	6478.0
22	7625.0
23	9204.0
24	10783.0
25	12807.5
26	19338.0
27	23844.0
28	28421.0
29	32998.0
30	38063.0
31	43128.0
32	48755.0
33	54382.0
34	62829.0
35	71276.0
36	75573.0
37	79870.0
38	87258.5
39	94647.0
40	102176.5
41	109706.0
42	123006.0
43	136306.0
44	149005.0
45	161704.0
46	182287.0
47	202870.0
48	217594.0
49	232318.0
50	218803.0
51	205288.0
52	175033.5
53	144779.0
54	124481.5
55	104184.0
56	90305.0
57	76426.0
58	67277.5
59	58129.0
60	52785.5
61	47442.0
62	39532.0
63	31622.0
64	26766.0
65	21910.0
66	16835.5
67	11761.0
68	9198.0
69	6635.0
70	5279.0
71	3923.0
72	3558.0
73	3193.0
74	2721.5
75	1877.5
76	1505.0
77	1095.5
78	686.0
79	586.5
80	487.0
81	345.5
82	204.0
83	144.5
84	85.0
85	49.0
86	13.0
87	9.0
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2054206.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.979395964580874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.24566324685568	21.359019492366734
2	11.68521138960478	7.00631158357584
3	4.654862008926503	4.18649853978276
4	2.3130129211109263	2.7737092093270537
5	1.3238599080787858	1.9844260192963783
6	0.8696113611202103	1.5642253998192548
7	0.6184571450815962	1.2978680147667792
8	0.490434166891847	1.1762336067048016
9	0.40234633624760574	1.0855890116338833
>10	5.90121424295243	42.962588173758334
>50	0.40666474830742705	7.494569791806787
>100	0.08267420132636022	4.231603043472158
>500	0.003825873344461231	0.7806402868627192
>1k	0.0016634231932440135	1.0688797692995735
>5k	4.99026957973204E-4	1.0278380575269903
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8969	0.43661638608786074	No Hit
TCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	5944	0.2893575425249464	No Hit
CTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGCT	5698	0.27738211260214407	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	4857	0.23644172006118178	No Hit
GCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTGC	4369	0.21268558265334636	No Hit
TCCTGTCTCTTATACACATCTGACGCCCTATCGTTCGTATGCCGTCTTCTG	2577	0.12544993053277032	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.736121888457146E-5	0.0	0.0	0.3004080408683452	0.0
2	9.736121888457146E-5	0.0	0.0	1.1322136144086814	0.0
3	9.736121888457146E-5	0.0	0.0	1.7048923038877308	0.0
4	9.736121888457146E-5	0.0	0.0	2.404481342182819	0.0
5	9.736121888457146E-5	0.0	0.0	3.444445201698369	0.0
6	9.736121888457146E-5	0.0	0.0	4.316314916809707	0.0
7	9.736121888457146E-5	0.0	0.0	5.078312496409805	0.0
8	9.736121888457146E-5	0.0	0.0	6.26086186098181	0.0
9	9.736121888457146E-5	0.0	0.0	6.918780297594302	0.0
10	9.736121888457146E-5	0.0	0.0	7.924473008062482	0.0
11	9.736121888457146E-5	0.0	0.0	9.887615945041539	0.0
12	1.4604182832685719E-4	0.0	0.0	11.6689854863631	0.0
13	1.4604182832685719E-4	0.0	0.0	12.229737426528791	0.0
14	1.4604182832685719E-4	0.0	0.0	12.417401175928802	0.0
15	1.4604182832685719E-4	0.0	0.0	12.650191850281812	0.0
16	1.4604182832685719E-4	0.0	0.0	13.225255889623533	0.0
17	1.4604182832685719E-4	0.0	0.0	14.144297115284445	0.0
18	1.4604182832685719E-4	0.0	0.0	15.150184548190396	0.0
19	1.4604182832685719E-4	0.0	0.0	15.930778120597447	0.0
20	1.4604182832685719E-4	0.0	0.0	16.700467236489427	0.0
21	1.4604182832685719E-4	0.0	0.0	17.762045286597353	0.0
22	1.4604182832685719E-4	0.0	0.0	18.92419747581304	0.0
23	1.4604182832685719E-4	0.0	0.0	20.029977519294558	0.0
24	1.4604182832685719E-4	0.0	0.0	20.8699614352212	0.0
25	1.4604182832685719E-4	0.0	0.0	21.555481777387467	0.0
26	1.4604182832685719E-4	0.0	0.0	22.18399712589682	0.0
27	1.4604182832685719E-4	0.0	0.0	22.802678991298826	0.0
28	1.4604182832685719E-4	0.0	0.0	23.467948199937105	0.0
29	1.4604182832685719E-4	0.0	0.0	24.19708636816366	0.0
30	1.4604182832685719E-4	0.0	0.0	24.99895336689699	0.0
31	1.4604182832685719E-4	0.0	0.0	25.732375428754466	0.0
32	1.947224377691429E-4	0.0	0.0	26.408938538783353	0.0
33	2.9208365665371437E-4	0.0	0.0	27.103221390649235	0.0
34	2.9208365665371437E-4	0.0	0.0	27.776425538626604	0.0
35	2.9208365665371437E-4	0.0	0.0	28.521628307969113	0.0
36	2.9208365665371437E-4	0.0	0.0	29.212600878392916	0.0
37	2.9208365665371437E-4	0.0	0.0	29.90225907236178	0.0
38	2.9208365665371437E-4	0.0	0.0	30.583836285163223	0.0
39	2.9208365665371437E-4	0.0	0.0	31.285226506007675	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGCA	20	7.0345856E-4	45.0	23
TAGCGCG	20	7.0345856E-4	45.0	1
CCCGATA	20	7.0345856E-4	45.0	16
CTTTACG	90	0.0	42.500004	1
CGTTTTT	5240	0.0	42.337788	1
CTCGCAC	45	1.929584E-8	40.000004	14
TAGGGCG	210	0.0	38.57143	5
CGTTTGG	1380	0.0	38.152176	2
TGGGCGA	3375	0.0	38.066666	6
TTTGGGA	16055	0.0	38.020866	4
AGGGCGA	280	0.0	37.767857	6
TTGGGAT	10165	0.0	37.629116	5
TTGGGAC	6070	0.0	37.47529	5
ACGGGAC	230	0.0	37.173912	5
TATGGGA	1800	0.0	37.0	4
CGACGGT	55	2.752131E-9	36.81818	28
TTTGGGC	4445	0.0	36.799774	4
TACGGGA	435	0.0	36.72414	4
TTTTGCG	1650	0.0	36.545452	1
TTGGGCG	2750	0.0	36.245453	5
>>END_MODULE