FastQCFastQC Report
Sat 18 Jun 2016
SRR3549510_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549510_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1348442
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC37300.2766155311092357No Hit
CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT36460.27038611968479176Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33480.24828654105997885No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC32050.2376817097064612No Hit
GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC27800.20616385428516762No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC27600.20468066108887145No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG27060.20067603945887177No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC24490.181617006886466No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAA207.033721E-445.00000415
CGTACAC253.8911654E-545.00000410
TGCGTAT351.2121382E-745.037
ACGGGTA556.184564E-1140.9090925
GCGTATT403.4588084E-739.37500438
CGTTTTT18550.038.6927261
CGGCCTA659.094947E-1238.07692745
TACGGGA2200.037.8409084
CGATGAA2200.037.84090819
TTTGGGA61950.037.59084
TTGGGAT26650.037.148225
TTTGGGC35200.037.1377834
ACGGGAT1400.036.9642835
TATGGGA7700.036.5259744
GCGATCT5800.036.0775839
TGGGCGA26300.036.017116
CGAACGA250.002107237236.00000422
GTTTACG250.002107237236.0000041
CGATTAT250.002107237236.00000411
AACGGAC250.002107237236.00000420