##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549510_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1348442 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.214692956760466 33.0 31.0 34.0 30.0 34.0 2 32.39614755399194 34.0 31.0 34.0 30.0 34.0 3 32.471385495260456 34.0 31.0 34.0 30.0 34.0 4 35.95140020853697 37.0 35.0 37.0 35.0 37.0 5 35.2170015469705 37.0 35.0 37.0 35.0 37.0 6 34.83506001741269 37.0 35.0 37.0 32.0 37.0 7 35.626410331330526 37.0 35.0 37.0 33.0 37.0 8 35.88203645392238 37.0 35.0 37.0 35.0 37.0 9 37.75939639969683 39.0 38.0 39.0 35.0 39.0 10 37.267309235399075 39.0 37.0 39.0 34.0 39.0 11 37.18881642666129 39.0 37.0 39.0 34.0 39.0 12 37.00475956696692 39.0 37.0 39.0 33.0 39.0 13 36.94408584128943 39.0 37.0 39.0 33.0 39.0 14 38.10387246911621 40.0 37.0 41.0 33.0 41.0 15 38.18696391835911 40.0 37.0 41.0 33.0 41.0 16 38.19509330026801 40.0 37.0 41.0 33.0 41.0 17 38.13672890639716 40.0 37.0 41.0 33.0 41.0 18 38.08481121175401 40.0 37.0 41.0 33.0 41.0 19 38.077214296202584 40.0 37.0 41.0 34.0 41.0 20 37.9680831656089 40.0 36.0 41.0 33.0 41.0 21 37.86759905134963 40.0 36.0 41.0 33.0 41.0 22 37.893201932304095 40.0 36.0 41.0 33.0 41.0 23 37.791885005065105 40.0 36.0 41.0 33.0 41.0 24 37.73499564682797 40.0 36.0 41.0 33.0 41.0 25 37.6315889003754 40.0 36.0 41.0 33.0 41.0 26 37.56442842925391 39.0 36.0 41.0 33.0 41.0 27 37.49794874380952 39.0 35.0 41.0 33.0 41.0 28 37.41794381960811 39.0 35.0 41.0 33.0 41.0 29 37.33306289777388 39.0 35.0 41.0 32.0 41.0 30 37.21580535165769 39.0 35.0 41.0 32.0 41.0 31 37.189169426642 39.0 35.0 41.0 32.0 41.0 32 37.143095513192264 39.0 35.0 41.0 32.0 41.0 33 37.00106567431154 39.0 35.0 41.0 31.0 41.0 34 36.878969951989035 39.0 35.0 41.0 31.0 41.0 35 36.74711407683831 39.0 35.0 41.0 31.0 41.0 36 36.59064090261205 39.0 35.0 41.0 31.0 41.0 37 36.56095998196437 39.0 35.0 41.0 31.0 41.0 38 36.47089826629547 39.0 35.0 41.0 30.0 41.0 39 36.39874165889226 39.0 35.0 41.0 30.0 41.0 40 36.277942247423326 38.0 35.0 40.0 30.0 41.0 41 36.16299477471037 38.0 35.0 40.0 30.0 41.0 42 36.08809351829741 38.0 35.0 40.0 30.0 41.0 43 35.922311081974605 38.0 35.0 40.0 29.0 41.0 44 35.85803838800631 38.0 35.0 40.0 29.0 41.0 45 35.846923338193264 38.0 35.0 40.0 29.0 41.0 46 35.603954786338605 38.0 34.0 40.0 28.0 41.0 47 35.496134798530456 38.0 34.0 40.0 28.0 41.0 48 35.3939220226009 37.0 34.0 40.0 28.0 41.0 49 35.427657993447255 37.0 34.0 40.0 28.0 41.0 50 35.22435670203094 37.0 34.0 40.0 28.0 41.0 51 34.10072735794346 36.0 33.0 39.0 24.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 6.0 9 15.0 10 31.0 11 27.0 12 20.0 13 17.0 14 43.0 15 58.0 16 93.0 17 180.0 18 328.0 19 598.0 20 1141.0 21 1768.0 22 2799.0 23 3904.0 24 5545.0 25 7909.0 26 10765.0 27 13431.0 28 15820.0 29 19047.0 30 23634.0 31 30218.0 32 39283.0 33 54722.0 34 92686.0 35 115352.0 36 121765.0 37 176164.0 38 273042.0 39 337918.0 40 112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 24.62056210055753 4.764832302761261 52.26913727101351 18.345468325667696 2 19.96133315337256 5.7833410706578405 53.03305592676585 21.222269849203748 3 20.873200330455443 5.840888966674132 51.359717362704515 21.92619334016591 4 18.663019988994705 5.087945940574381 51.63425642333893 24.614777647091977 5 19.180135296883368 7.480855683818807 48.00925809193128 25.329750927366547 6 22.208074207121996 8.486683149887055 51.93089506259817 17.37434758039278 7 83.61842778554806 2.1450681601433357 9.796416901876388 4.4400871524322145 8 85.44905898807662 3.5315571600409954 6.907527353790523 4.111856498091872 9 79.89042168665765 5.072298252353457 8.776276621463882 6.261003439525021 10 40.25564317931361 26.774455260218833 16.324914234353425 16.644987326114137 11 31.821613387895063 23.875257519418707 23.857162562423895 20.445966530262332 12 27.668820757585422 21.385050302497252 29.98356621938504 20.962562720532286 13 24.7435929761903 22.17307084768941 31.86099216725673 21.222344008863562 14 21.383863747940214 24.297003504785522 31.13645229086605 23.18268045640821 15 19.7241705612848 22.74714077431584 34.99164220633887 22.537046458060487 16 21.68680595828371 21.315859339890036 32.334279116194836 24.66305558563142 17 21.83638599213018 21.059637715229872 30.51877648426851 26.585199808371442 18 22.802686359517132 21.44163412293595 30.513956106380547 25.241723411166365 19 24.457262529645323 23.838400168490747 28.461365042026276 23.24297225983765 20 27.42209156938155 23.323732129375976 28.07573481098927 21.178441490253196 21 27.125675409101763 24.21735603014442 27.05470461465899 21.602263946094826 22 25.110090014995084 22.61357922698937 26.93375020950104 25.34258054851451 23 24.233745314963492 24.779041293581777 26.890960085787896 24.09625330566684 24 24.314356865182187 22.648360107442514 28.41405117906443 24.623231848310866 25 24.186283132682014 24.017124948644437 26.524759685622367 25.271832233051178 26 22.899019757616568 23.79679659933464 27.629367818563942 25.67481582448485 27 22.674390148037514 24.164702671675904 28.258241733793522 24.902665446493064 28 21.34507824585707 23.905959618582038 29.48728977590434 25.26167235965655 29 23.54680438609892 23.142189282149324 28.582541926163675 24.72846440558808 30 23.37008191676023 23.222874992027837 29.8035807250145 23.603462366197434 31 25.02984926307546 23.93821907060148 26.449561790570154 24.582369875752903 32 26.03582504846334 24.967629308490835 25.85509795749465 23.141447685551178 33 24.89658435438825 23.946005834882033 27.307737373947116 23.849672436782598 34 23.721821183261866 23.286281501169498 28.249713372914815 24.742183942653817 35 24.51792513137384 22.968803997502302 27.9862241015928 24.527046769531058 36 24.264150775487565 25.63818095253633 26.990408189599552 23.10726008237655 37 24.170042167182572 25.481110793048572 28.07922031500057 22.269626724768287 38 23.678215303290763 25.517893984316714 26.691915558844947 24.111975153547576 39 24.20148586294405 24.07548860091869 28.0793686343202 23.64365690181706 40 24.246500776451636 23.127283190526548 29.95486643103671 22.671349601985106 41 22.030165183226273 24.10589406144276 28.758003681285516 25.105937074045453 42 23.591003543348545 24.289587538804042 26.915506933186595 25.203901984660813 43 23.383430655526897 23.51209766530559 28.160350982837972 24.94412069632954 44 23.002917441017114 23.103032981767104 28.165023041406307 25.729026535809474 45 24.12435981673665 22.586807589796223 27.169429608392498 26.119402985074625 46 24.044119064817025 23.264775199823205 28.724780153688478 23.966325581671292 47 22.04774102260238 23.512320144285034 30.5237451814761 23.91619365163648 48 22.809360728900465 22.87944160742546 29.56500909939026 24.74618856428382 49 22.473936587558086 22.49240234285197 30.266856119877605 24.766804949712334 50 22.324059915072358 22.44612671512753 29.314942726494724 25.914870643305388 51 21.570820250333348 22.413496464809015 28.29947450465055 27.716208780207086 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 220.0 1 1045.5 2 1871.0 3 4250.0 4 6629.0 5 4725.5 6 2822.0 7 2699.5 8 2577.0 9 2639.5 10 2702.0 11 2701.5 12 2701.0 13 2725.0 14 2749.0 15 2761.5 16 2774.0 17 2714.0 18 2654.0 19 2797.5 20 2941.0 21 3124.5 22 3308.0 23 3826.5 24 4345.0 25 5291.5 26 7358.5 27 8479.0 28 11231.0 29 13983.0 30 15544.5 31 17106.0 32 19892.5 33 22679.0 34 27360.5 35 32042.0 36 33748.5 37 35455.0 38 38255.5 39 41056.0 40 46562.5 41 52069.0 42 58113.0 43 64157.0 44 72324.0 45 80491.0 46 96367.5 47 112244.0 48 119041.5 49 125839.0 50 123162.5 51 120486.0 52 109524.0 53 98562.0 54 90609.5 55 82657.0 56 78968.5 57 75280.0 58 73993.0 59 72706.0 60 70176.0 61 67646.0 62 62220.5 63 56795.0 64 49229.5 65 41664.0 66 34664.5 67 27665.0 68 23565.0 69 19465.0 70 16857.0 71 14249.0 72 11588.0 73 8927.0 74 7303.5 75 4579.5 76 3479.0 77 2551.0 78 1623.0 79 1243.5 80 864.0 81 590.5 82 317.0 83 233.5 84 150.0 85 94.0 86 38.0 87 36.0 88 34.0 89 22.5 90 11.0 91 9.5 92 8.0 93 6.5 94 5.0 95 2.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1348442.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.21451671122839 #Duplication Level Percentage of deduplicated Percentage of total 1 76.38404471821626 23.079069956097616 2 9.911051539486943 5.989152647313482 3 3.47687810219912 3.15156574565398 4 1.5827211470640246 1.9128461818872216 5 0.9144053253358342 1.3814157491597894 6 0.596233856460049 1.080895069188738 7 0.4575561729995883 0.9677387044795238 8 0.35074020553549384 0.8477956641161487 9 0.29391889853452885 0.7992555724365626 >10 5.297454212121031 42.88325314774652 >50 0.6672555450225619 12.226098382434643 >100 0.06239874982646626 3.1637167589214137 >500 0.002913560289826429 0.5456505399165752 >1k 0.0024279669081886906 1.9715458806477495 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC 3730 0.2766155311092357 No Hit CTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGCT 3646 0.27038611968479176 Illumina Single End Adapter 1 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3348 0.24828654105997885 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGC 3205 0.2376817097064612 No Hit GAATCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTC 2780 0.20616385428516762 No Hit GCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC 2760 0.20468066108887145 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCG 2706 0.20067603945887177 No Hit TCTGTCTCTTATACACATCTGACGCGTCGTTGATCGTATGCCGTCTTCTGC 2449 0.181617006886466 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 7.41596598148085E-5 0.0 0.0 0.2925598579694195 0.0 2 7.41596598148085E-5 0.0 0.0 1.0321541453025047 0.0 3 7.41596598148085E-5 0.0 0.0 1.4536034920300613 0.0 4 7.41596598148085E-5 0.0 0.0 1.9736110266514986 0.0 5 7.41596598148085E-5 0.0 0.0 2.931160554180306 0.0 6 7.41596598148085E-5 0.0 0.0 3.4897311119054435 0.0 7 7.41596598148085E-5 0.0 0.0 4.0339888552863234 0.0 8 7.41596598148085E-5 0.0 0.0 4.857383558210142 0.0 9 7.41596598148085E-5 0.0 0.0 5.274679963988069 0.0 10 7.41596598148085E-5 0.0 0.0 6.254477389461319 0.0 11 7.41596598148085E-5 0.0 0.0 7.5367720673191725 0.0 12 1.48319319629617E-4 0.0 0.0 9.044957068972932 0.0 13 1.48319319629617E-4 0.0 0.0 9.432218812525862 0.0 14 2.224789794444255E-4 0.0 0.0 9.58038981283585 0.001038235237407319 15 2.224789794444255E-4 0.0 0.0 9.840022781847495 0.001038235237407319 16 2.224789794444255E-4 0.0 0.0 10.360846072726895 0.001038235237407319 17 2.224789794444255E-4 0.0 0.0 11.07715422687813 0.001038235237407319 18 2.224789794444255E-4 0.0 0.0 11.85026868044751 0.001038235237407319 19 2.224789794444255E-4 0.0 0.0 12.443249320326718 0.001038235237407319 20 2.224789794444255E-4 0.0 0.0 13.013092146343705 0.001038235237407319 21 2.224789794444255E-4 0.0 0.0 13.786058280593455 0.001038235237407319 22 2.224789794444255E-4 0.0 0.0 14.609452983517274 0.001038235237407319 23 2.224789794444255E-4 0.0 0.0 15.386572058716652 0.001038235237407319 24 2.224789794444255E-4 0.0 0.0 16.01611341088456 0.001038235237407319 25 2.96638639259234E-4 0.0 0.0 16.557182288893404 0.001038235237407319 26 2.96638639259234E-4 0.0 0.0 17.037885203812994 0.001038235237407319 27 2.96638639259234E-4 0.0 0.0 17.4951536662311 0.001038235237407319 28 2.96638639259234E-4 0.0 0.0 17.968069816870138 0.001038235237407319 29 2.96638639259234E-4 0.0 0.0 18.464865377969538 0.001038235237407319 30 2.96638639259234E-4 0.0 0.0 19.033002531810787 0.001038235237407319 31 2.96638639259234E-4 0.0 0.0 19.551156074936852 0.001038235237407319 32 2.96638639259234E-4 0.0 0.0 20.066639870309587 0.001038235237407319 33 2.96638639259234E-4 0.0 0.0 20.569961481472692 0.001038235237407319 34 2.96638639259234E-4 0.0 0.0 21.06898183236654 0.001038235237407319 35 3.707982990740425E-4 0.0 0.0 21.61494524792316 0.001038235237407319 36 3.707982990740425E-4 0.0 0.0 22.13777084961756 0.001038235237407319 37 3.707982990740425E-4 0.0 0.0 22.640795822141406 0.001038235237407319 38 3.707982990740425E-4 0.0 0.0 23.169999154579877 0.001038235237407319 39 3.707982990740425E-4 0.0 0.0 23.700314881915574 0.001038235237407319 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTAA 20 7.033721E-4 45.000004 15 CGTACAC 25 3.8911654E-5 45.000004 10 TGCGTAT 35 1.2121382E-7 45.0 37 ACGGGTA 55 6.184564E-11 40.909092 5 GCGTATT 40 3.4588084E-7 39.375004 38 CGTTTTT 1855 0.0 38.692726 1 CGGCCTA 65 9.094947E-12 38.076927 45 TACGGGA 220 0.0 37.840908 4 CGATGAA 220 0.0 37.840908 19 TTTGGGA 6195 0.0 37.5908 4 TTGGGAT 2665 0.0 37.14822 5 TTTGGGC 3520 0.0 37.137783 4 ACGGGAT 140 0.0 36.964283 5 TATGGGA 770 0.0 36.525974 4 GCGATCT 580 0.0 36.077583 9 TGGGCGA 2630 0.0 36.01711 6 CGAACGA 25 0.0021072372 36.000004 22 GTTTACG 25 0.0021072372 36.000004 1 CGATTAT 25 0.0021072372 36.000004 11 AACGGAC 25 0.0021072372 36.000004 20 >>END_MODULE