FastQCFastQC Report
Sat 18 Jun 2016
SRR3549509_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549509_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences784538
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC21280.2712424382247896No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC20080.255946812009106No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT19380.24702436338329054Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.22828722126907813No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC16100.20521631839375531No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTT207.0319156E-445.030
CGAACAT207.0319156E-445.034
TCCGATC207.0319156E-445.014
CGGTTAG253.889669E-545.025
GGCGTAA253.889669E-545.022
TTCGTCA253.889669E-545.011
CGACAAT253.889669E-545.020
CCGTCGG253.889669E-545.02
CGTTACA253.889669E-545.018
TATTACG207.0319156E-445.01
TTACGTA253.889669E-545.042
ACGTAAC207.0319156E-445.031
TATGTCG207.0319156E-445.016
TCCGGTA406.8102963E-945.04
GATACGA302.1646174E-644.9999969
CACAACG650.044.99999612
CGATATC302.1646174E-644.99999610
TACGGGA1900.041.4473724
CGGGTAT556.002665E-1140.9090926
TCTTACG501.0804797E-940.51
Produced by FastQC (version 0.11.5)