##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549509_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	784538
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.31799479438855	33.0	31.0	34.0	31.0	34.0
2	32.50976880660975	34.0	31.0	34.0	31.0	34.0
3	32.5787750242818	34.0	31.0	34.0	31.0	34.0
4	36.025253078882095	37.0	35.0	37.0	35.0	37.0
5	35.25124723085434	37.0	35.0	37.0	35.0	37.0
6	34.975396985232074	37.0	35.0	37.0	33.0	37.0
7	35.75963178329157	37.0	35.0	37.0	35.0	37.0
8	36.03789873785591	37.0	35.0	37.0	35.0	37.0
9	37.91086728749914	39.0	38.0	39.0	35.0	39.0
10	37.414941022614585	39.0	37.0	39.0	35.0	39.0
11	37.28336422200072	39.0	37.0	39.0	34.0	39.0
12	37.14490311495428	39.0	37.0	39.0	34.0	39.0
13	37.08089729241923	39.0	37.0	39.0	33.0	39.0
14	38.25245813459641	40.0	37.0	41.0	33.0	41.0
15	38.36642329625843	40.0	38.0	41.0	34.0	41.0
16	38.375496151875375	40.0	38.0	41.0	34.0	41.0
17	38.32510598594332	40.0	37.0	41.0	34.0	41.0
18	38.27994692417703	40.0	37.0	41.0	34.0	41.0
19	38.288095669043436	40.0	37.0	41.0	34.0	41.0
20	38.21985168341113	40.0	37.0	41.0	34.0	41.0
21	38.12316675546627	40.0	37.0	41.0	34.0	41.0
22	38.15548004048242	40.0	37.0	41.0	34.0	41.0
23	38.08678228460572	40.0	37.0	41.0	34.0	41.0
24	38.033076791691414	40.0	37.0	41.0	34.0	41.0
25	37.92721576265267	40.0	36.0	41.0	34.0	41.0
26	37.90167716541455	40.0	36.0	41.0	34.0	41.0
27	37.87923848175614	40.0	36.0	41.0	33.0	41.0
28	37.77930450787597	40.0	36.0	41.0	33.0	41.0
29	37.76054824622899	40.0	36.0	41.0	33.0	41.0
30	37.721183677527414	40.0	36.0	41.0	33.0	41.0
31	37.64518735867479	40.0	36.0	41.0	33.0	41.0
32	37.60608663952543	40.0	36.0	41.0	33.0	41.0
33	37.492919399697655	40.0	36.0	41.0	33.0	41.0
34	37.39856323084414	40.0	35.0	41.0	33.0	41.0
35	37.289190580953374	40.0	35.0	41.0	32.0	41.0
36	37.16534826866258	39.0	35.0	41.0	32.0	41.0
37	37.148069309580926	39.0	35.0	41.0	32.0	41.0
38	37.04403865714599	39.0	35.0	41.0	32.0	41.0
39	37.002917640700645	39.0	35.0	41.0	31.0	41.0
40	36.92525282395499	39.0	35.0	41.0	31.0	41.0
41	36.835457300984785	39.0	35.0	41.0	31.0	41.0
42	36.732321442683464	39.0	35.0	41.0	31.0	41.0
43	36.5927310085681	39.0	35.0	41.0	31.0	41.0
44	36.52089255077511	39.0	35.0	40.0	31.0	41.0
45	36.52334749878273	39.0	35.0	40.0	31.0	41.0
46	36.325260216840995	38.0	35.0	40.0	30.0	41.0
47	36.242592455687294	38.0	35.0	40.0	30.0	41.0
48	36.16673889601269	38.0	35.0	40.0	30.0	41.0
49	36.19425062903263	38.0	35.0	40.0	30.0	41.0
50	36.020089530398785	38.0	35.0	40.0	30.0	41.0
51	34.987672999905676	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	13.0
10	8.0
11	16.0
12	8.0
13	20.0
14	25.0
15	21.0
16	33.0
17	56.0
18	111.0
19	225.0
20	337.0
21	623.0
22	1004.0
23	1545.0
24	2202.0
25	3334.0
26	4923.0
27	6213.0
28	7386.0
29	8956.0
30	11091.0
31	14268.0
32	19309.0
33	28055.0
34	49072.0
35	64918.0
36	65004.0
37	97136.0
38	167855.0
39	230681.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.928271160861552	4.289021054429486	55.37348095312146	17.409226831587507
2	19.768959566012096	4.2353588991227955	54.863499282380204	21.132182252484903
3	20.241594416076722	4.300747701194843	53.390530477809875	22.067127404918562
4	18.21110003594472	4.747507450244603	53.226484886646666	23.81490762716401
5	17.076292034292795	7.549921100061438	51.32702303776235	24.046763827883417
6	21.826731146228738	7.0781784948593955	53.84914433717678	17.245946021735083
7	85.73415181928728	1.5217873449087234	8.63616548847857	4.1078953473254325
8	88.22249527747542	2.2181207283777207	5.988620054095532	3.570763940051342
9	82.58860628803194	5.310513958533558	7.755519809110585	4.345359944323921
10	39.19364007861952	33.49984321983129	13.956239213396929	13.350277488152262
11	28.487976363158957	25.233576958668667	27.088808955079298	19.18963772309308
12	25.32025217389088	23.25916654132751	31.134119698472222	20.286461586309397
13	23.2548327805664	24.965521109238814	32.391037782746025	19.388608327448768
14	19.77278347256602	27.067522541929133	31.69840084227915	21.461293143225696
15	18.45149629463455	24.355353086784834	35.89641292072532	21.2967376978553
16	21.624064098870928	23.019535063948464	33.63763131932424	21.71876951785637
17	21.321593090455785	22.050557143184907	30.784869566547446	25.842980199811866
18	22.572775314898706	22.698709304074498	31.129658474159317	23.598856906867482
19	23.941733861202387	24.298249415579615	30.10829303360704	21.651723689610954
20	26.83923532066006	24.835890677060895	28.28416214383497	20.040711858444077
21	25.99058299279321	25.10267189097278	28.522901376351435	20.383843739882582
22	23.85760791701613	23.67329562111714	28.052433406667365	24.41666305519937
23	22.08089346851268	25.819399442729353	28.440687385442132	23.65901970331584
24	22.208611947413637	24.640616515707332	30.885438308915564	22.265333227963463
25	22.340791650627505	25.156716436934857	28.537942075463523	23.96454983697412
26	20.94022214347807	26.935597765818862	28.835059614703173	23.289120475999887
27	21.06602866910207	25.871532035414475	30.36016101195863	22.702278283524826
28	19.07797965171859	26.70093736695992	31.82216795107439	22.398915030247103
29	20.265302636711034	24.787581990929695	31.149925178895092	23.79719019346418
30	22.584501961664063	24.015917648348456	31.1352668704384	22.264313519549084
31	24.14809735156232	25.496661729578427	27.70012414949945	22.6551167693598
32	24.3183886567636	26.31357563304773	27.42747961220489	21.940556097983784
33	23.60229842276601	25.245431068985823	27.611409517448486	23.54086099079968
34	21.93048647739179	25.092474806828985	29.67071066028669	23.306328055492532
35	21.6930218803933	24.656931850337397	30.22288276667287	23.427163502596432
36	22.282668271007903	26.031498792920164	29.576515095508437	22.10931784056349
37	22.977854482510725	26.841912055247803	28.996683398382235	21.18355006385924
38	22.367813923608544	27.505614769456677	27.50586969656027	22.620701610374514
39	23.603573058283985	24.493905967588567	28.15886547241816	23.743655501709284
40	24.37447261955444	23.995268552957285	28.89292806721918	22.7373307602691
41	21.75790082825816	24.07480580927884	29.62762797977918	24.539665382683822
42	22.476922723947087	25.00197568505286	29.126313830560154	23.394787760439904
43	22.405543134940565	24.39767098598156	29.34478635834083	23.851999520737046
44	21.56683296411391	25.474483071565686	29.06347429952405	23.89520966479635
45	22.771363528598997	24.167854202090915	27.59101534916091	25.469766920149183
46	23.02437867891676	24.520290922810624	28.672288659057944	23.78304173921467
47	21.220004639673284	25.196102674440247	31.273309897034945	22.310582788851526
48	21.858087179970887	24.027771758665608	30.460474827223155	23.653666234140346
49	21.995492888808446	23.317799775154295	30.967142445617675	23.719564890419583
50	22.09096308910467	23.575786003992157	29.23363814117353	25.09961276572964
51	21.122877413203696	23.506063441159	28.230627452080075	27.140431693557225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	98.0
1	449.0
2	800.0
3	2153.5
4	3507.0
5	2454.5
6	1402.0
7	1378.0
8	1354.0
9	1368.5
10	1383.0
11	1479.0
12	1575.0
13	1570.5
14	1566.0
15	1606.5
16	1647.0
17	1627.0
18	1607.0
19	1596.5
20	1586.0
21	1869.0
22	2152.0
23	3141.0
24	4130.0
25	4788.0
26	6850.5
27	8255.0
28	9031.5
29	9808.0
30	11518.0
31	13228.0
32	15884.0
33	18540.0
34	20451.5
35	22363.0
36	24433.5
37	26504.0
38	28790.5
39	31077.0
40	35346.5
41	39616.0
42	44475.5
43	49335.0
44	53884.0
45	58433.0
46	62426.0
47	66419.0
48	72612.5
49	78806.0
50	76433.5
51	74061.0
52	65067.5
53	56074.0
54	49836.5
55	43599.0
56	41145.5
57	38692.0
58	35394.5
59	32097.0
60	30383.0
61	28669.0
62	24371.5
63	20074.0
64	16887.5
65	13701.0
66	11611.0
67	9521.0
68	7713.0
69	5905.0
70	5010.0
71	4115.0
72	3316.5
73	2518.0
74	2281.5
75	1689.0
76	1333.0
77	1049.5
78	766.0
79	640.5
80	515.0
81	326.5
82	138.0
83	84.0
84	30.0
85	24.0
86	18.0
87	12.5
88	7.0
89	5.0
90	3.0
91	10.5
92	18.0
93	10.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	784538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.555780053155388
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.62044768122735	22.05465539134345
2	10.881225331387705	6.432062052066357
3	3.8372347183347317	3.40237396042299
4	1.751858621443152	2.0711019239839126
5	0.9894271401516287	1.4621645466472044
6	0.6576300851977057	1.166206209266474
7	0.4644659272644035	0.9609356951888103
8	0.3523640479308073	0.8331515439425955
9	0.2692394963305259	0.7161825001650626
>10	5.363538615356888	43.066943404407034
>50	0.7458521983966754	13.287850348204453
>100	0.06372246416529108	3.1876936768788147
>500	8.553350894669943E-4	0.16103421009332683
>1k	0.002138337723667486	1.1976445373895381
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	2128	0.2712424382247896	No Hit
TCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	2008	0.255946812009106	No Hit
CTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGCT	1938	0.24702436338329054	Illumina Single End Adapter 1 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1791	0.22828722126907813	No Hit
GCTGTCTCTTATACACATCTGACGCTTAGCGGTTCGTATGCCGTCTTCTGC	1610	0.20521631839375531	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2746355179736355E-4	0.0	0.0	0.26448686997952936	0.0
2	1.2746355179736355E-4	0.0	0.0	1.077067012687722	0.0
3	1.2746355179736355E-4	0.0	0.0	1.5503391805113327	0.0
4	1.2746355179736355E-4	0.0	0.0	2.0526730381447424	0.0
5	1.2746355179736355E-4	0.0	0.0	2.85582087801993	0.0
6	1.2746355179736355E-4	0.0	0.0	3.407992984406109	0.0
7	1.2746355179736355E-4	0.0	0.0	3.9149155299042238	0.0
8	1.2746355179736355E-4	0.0	0.0	4.726985818405227	0.0
9	1.2746355179736355E-4	0.0	0.0	5.211602242338803	0.0
10	2.549271035947271E-4	0.0	0.0	6.0092691494867045	0.0
11	2.549271035947271E-4	0.0	0.0	7.32061417037798	0.0
12	2.549271035947271E-4	0.0	0.0	8.557138086364205	0.0
13	2.549271035947271E-4	0.0	0.0	8.949598362348286	0.0
14	2.549271035947271E-4	0.0	0.0	9.074767570213298	0.0
15	2.549271035947271E-4	0.0	0.0	9.280238815710648	0.0
16	2.549271035947271E-4	0.0	0.0	9.796848591145363	0.0
17	2.549271035947271E-4	0.0	0.0	10.577180455248822	0.0
18	2.549271035947271E-4	0.0	0.0	11.478475230007978	0.0
19	2.549271035947271E-4	0.0	0.0	12.032814216774714	0.0
20	2.549271035947271E-4	0.0	0.0	12.642854775676895	0.0
21	2.549271035947271E-4	0.0	0.0	13.414646581809931	0.0
22	2.549271035947271E-4	0.0	0.0	14.262279201262398	0.0
23	2.549271035947271E-4	0.0	0.0	15.06721153086275	0.0
24	2.549271035947271E-4	0.0	0.0	15.686302001942545	0.0
25	2.549271035947271E-4	0.0	0.0	16.200744896996703	0.0
26	2.549271035947271E-4	0.0	0.0	16.665731933953484	0.0
27	2.549271035947271E-4	0.0	0.0	17.135562585878567	0.0
28	2.549271035947271E-4	0.0	0.0	17.614953004188454	0.0
29	2.549271035947271E-4	0.0	0.0	18.187391815310413	0.0
30	2.549271035947271E-4	0.0	0.0	18.828915871506542	0.0
31	2.549271035947271E-4	0.0	0.0	19.3869513012754	0.0
32	2.549271035947271E-4	0.0	0.0	19.91146381692155	0.0
33	2.549271035947271E-4	0.0	0.0	20.403855517514767	0.0
34	2.549271035947271E-4	0.0	0.0	20.948379810793103	0.0
35	2.549271035947271E-4	0.0	0.0	21.491629468553466	0.0
36	2.549271035947271E-4	0.0	0.0	22.02136798982331	0.0
37	2.549271035947271E-4	0.0	0.0	22.53759537460263	0.0
38	2.549271035947271E-4	0.0	0.0	23.044008065893557	0.0
39	2.549271035947271E-4	0.0	0.0	23.584835916169773	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.0319156E-4	45.0	30
CGAACAT	20	7.0319156E-4	45.0	34
TCCGATC	20	7.0319156E-4	45.0	14
CGGTTAG	25	3.889669E-5	45.0	25
GGCGTAA	25	3.889669E-5	45.0	22
TTCGTCA	25	3.889669E-5	45.0	11
CGACAAT	25	3.889669E-5	45.0	20
CCGTCGG	25	3.889669E-5	45.0	2
CGTTACA	25	3.889669E-5	45.0	18
TATTACG	20	7.0319156E-4	45.0	1
TTACGTA	25	3.889669E-5	45.0	42
ACGTAAC	20	7.0319156E-4	45.0	31
TATGTCG	20	7.0319156E-4	45.0	16
TCCGGTA	40	6.8102963E-9	45.0	4
GATACGA	30	2.1646174E-6	44.999996	9
CACAACG	65	0.0	44.999996	12
CGATATC	30	2.1646174E-6	44.999996	10
TACGGGA	190	0.0	41.447372	4
CGGGTAT	55	6.002665E-11	40.909092	6
TCTTACG	50	1.0804797E-9	40.5	1
>>END_MODULE