SRR3549508_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549508_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1286278
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3316	0.2577980809747193	No Hit
CCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2569	0.1997235434330681	No Hit
CTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGCT	2444	0.19000558199704887	TruSeq Adapter, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2420	0.18813973340133314	No Hit
GCTGTCTCTTATACACATCTGACGCGGAGTTACTCGTATGCCGTCTTCTGC	2368	0.18409706144394913	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	20	7.0336007E-4	45.0	45
TACGATC	20	7.0336007E-4	45.0	12
ACTACGG	20	7.0336007E-4	45.0	32
GTATACG	20	7.0336007E-4	45.0	1
ATAACGC	20	7.0336007E-4	45.0	11
ATACGAT	20	7.0336007E-4	45.0	11
TATACGG	25	3.8910664E-5	45.0	2
TTCGAAT	25	3.8910664E-5	45.0	20
TCGAATC	25	3.8910664E-5	45.0	21
TACGGGT	100	0.0	42.75	4
GCGATCA	60	3.6379788E-12	41.249996	9
GCGATAT	50	1.0822987E-9	40.5	9
TTTGGGC	3640	0.0	39.00412	4
CCGTTTG	185	0.0	38.918922	1
TTTGGGA	6435	0.0	38.251747	4
ATAGCGC	30	1.1399976E-4	37.499996	36
GTTCACG	30	1.1399976E-4	37.499996	37
ACGTACC	30	1.1399976E-4	37.499996	11
TTGGGAC	2370	0.0	37.40506	5
TACGAAT	290	0.0	37.24138	12