SRR3549506_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549506_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	950787
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3983	0.4189161189625016	RNA PCR Primer, Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGCT	3966	0.41712812648889813	TruSeq Adapter, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3542	0.372533490676671	Illumina PCR Primer Index 2 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTGC	3468	0.3647504646151031	Illumina PCR Primer Index 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2085	0.21929201808606977	No Hit
TTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCGG	1691	0.1778526631096134	No Hit
TTTTCTGGGGTCTGATGAGCGTCGGCATCGGGCGCCTTAACCCGGCGTTCG	1463	0.15387252875775542	No Hit
TCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1310	0.1377805964953244	TruSeq Adapter, Index 10 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCTGATTACCTCGTATGCCGTCTTCTG	1041	0.10948824500124635	Illumina PCR Primer Index 2 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAGTTA	20	7.0326723E-4	45.0	33
CGTTGAA	25	3.890296E-5	45.0	44
ATTACGA	20	7.0326723E-4	45.0	10
AAGCGTA	20	7.0326723E-4	45.0	41
ATAGACG	25	3.890296E-5	45.0	15
TAACGTT	20	7.0326723E-4	45.0	45
AGTGCGT	20	7.0326723E-4	45.0	8
TCGTAAG	25	3.890296E-5	45.0	21
CGTAAGC	25	3.890296E-5	45.0	22
CAGTCGA	20	7.0326723E-4	45.0	23
CCGCGAC	20	7.0326723E-4	45.0	42
TGAATCG	20	7.0326723E-4	45.0	31
ACGAGTT	20	7.0326723E-4	45.0	32
CAATCCA	25	3.890296E-5	45.0	33
CGATATA	25	3.890296E-5	45.0	35
GCCGTAT	20	7.0326723E-4	45.0	9
GTCACGC	30	2.165105E-6	44.999996	17
CGGTCTA	30	2.165105E-6	44.999996	31
AATGCGA	90	0.0	42.5	25
ATGCGAA	85	0.0	42.35294	26